Kutkoside
Kutkoside is an orally active iridoid glycoside mixture composed of picroside II, picroside IV, and 6-ferulloylcatalpol. Kutkoside exerts antioxidant effects by targeting hepatic and lysosomal enzymes, inhibiting Xanthine Oxidase (IC50 = 354.6 μM) and lipid peroxidation, and downregulates MMP-1/2/9/13 to suppress tumor invasion and inflammatory mediators. Kutkoside can be used in research on D-galactosamine-induced liver injury and breast cancer.
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- CAS No.: 35988-27-3
- Formule: C23H28O13
- Masse moléculaire:512.46
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
- Protocole
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
Description
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Xanthine oxidase 354.6 μM (IC50) |
MMP-1 |
MMP-2 |
MMP-9 |
MMP13 |
In Vitro
Kutkoside inhibits Xanthine oxidase, Microorganism (HY-P2755) activity in the xanthine-xanthine oxidase assay, with an IC50 of 354.6 μM[2].
Kutkoside inhibits superoxide anion reduction of Nitro blue tetrazolium chloride (NBT) (HY-15925) in the xanthine-xanthine oxidase system, with an IC50 of 291.5 μM[2].
Kutkoside (90 s) inhibits non-enzymatic superoxide anion generation produced by Phenazine methylsulfate (HY-W004520)/NADH (HY-113355), with an IC50 of 372.3 μM[2].
Kutkoside (37°C; 90 min) inhibits MDA production from enzymatic lipid peroxidation in rat liver microsomes with an IC50 of 366.9 μM[2].
Kutkoside (37°C; 90 min) inhibits MDA formation caused by non-enzymatic ascorbic acid/Fe2+-stimulated lipid peroxidation in boiled rat liver microsomes, with an IC50 of 561.5 μM[2].
Kutkoside (5-10 µM; 48 h) reduces MCF-7 viability to 42.60% at 5 µM and to 28.23% at 10 µM in the MTT assay[4].
Kutkoside (5 µM; 48 h) induces PI uptake in MCF-7 cells, indicating loss of membrane integrity[4].
Kutkoside (5-10 µM; 48 hours) inhibits MCF-7 migration and invasion in the wound scratch assay[4].
Kutkoside (5 µM; 48 h) downregulates gelatinase and collagenase MMP activity in MCF-7 conditioned medium[4].
Kutkoside (5 µM; 18 h) downregulates MMP-2, MMP-9, MMP-1, and MMP-13 mRNA expression in MCF-7 cells[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:MCF-7 human breast cancer cells
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Concentration:5, 10 µM
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Incubation Time:48 h
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Result:Reduced MCF-7 viability to 42.60% at 5 µM and to 28.23% at 10 µM in the MTT assay.
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Cell Line:MCF-7 human breast cancer cells
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Concentration:5, 10 µM
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Incubation Time:48 h
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Result:Induced PI uptake in MCF-7 cells, indicating loss of membrane integrity.
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Cell Line:MCF-7 human breast cancer cells
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Concentration:5, 10 µM
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Incubation Time:48 h (Kutkoside); 24 h (post-scratch incubation)
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Result:Significantly blocked MCF-7 tumor invasion and suppressed migration across the wounded space in a dose-dependent manner.
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Cell Line:MCF-7 human breast cancer cells
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Concentration:5 µM
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Incubation Time:18 h
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Result:Suppressed relative mRNA expression of gelatinases MMP-2 and MMP-9 and collagenases MMP-1 and MMP-13 in MCF-7 cells.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Sprague Dawley (adult male albino, 120 g)[1]
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Dosage:12 mg/kg/day
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Administration:p.o. (gavage); daily; 7 days
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Result:Exerted protective effects against galactosamine-induced hepatic and serum biochemical alterations in Sprague Dawley rats.
- Ex Vivo Tissue Slice/Explant Invasion Assay
- Mitochondrial membrane-potential fluorescent assay
- Mitochondrial membrane-potential and mitochondrial mass staining
- Organotypic 3D Invasion Assay
- Cell invasion
- Lysosome and acidic-vesicle live-cell staining
- Ferroptosis Solutions
- Breast Cancer Modeling
- 3D Tumor Spheroid Invasion Assay
Chemical Information
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CAS No. 35988-27-3
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Masse moléculaire 512.46
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Formule C23H28O13
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SMILES
O=C(C1=CC(OC)=C(C=C1)O)OC[C@@]23[C@]4([H])[C@](C=CO[C@H]4O[C@@H]5O[C@@H]([C@H]([C@@H]([C@H]5O)O)O)CO)([H])[C@@H]([C@]2([H])O3)O
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Structure Classification
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Initial Source
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureté et documentation
Références
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Kutkoside Related Classifications
HY-N22071 Related Classifications
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