Multi-kinase-IN-4
Multi-kinase-IN-4 (compound 5d) is multi-targeted kinase inhibitor, including VEGFR2, EGFR, HER2, and CDK2, with IC50 values of 0.33, 0.22, 0.18 and 2.09 μM, respectively. Multi-kinase-IN-4 shows broad-spectrum anti-cancer activities against HepG2, MCF-7, MDA-231, and HeLa cell lines (IC50 = 1.94–7.1 µM), but exhibits lower toxicity in the WI-38 cells (IC50 = 40.85 µM). Multi-kinase-IN-4 induces apoptosis and arrests cell cycle at S phase in HepG2 cells. Multi-kinase-IN-4 has the potential for the research of cancer.
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- Formule: C21H20ClFN2OS
- Masse moléculaire:402.91
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Activité biologique
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Chemical Information
- Protocole
- Pureté et documentation
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Activité biologique
Description
IC50 & Target
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VEGFR2 0.33 μM (IC50) |
CDK2 2.09 μM (IC50) |
HER2 0.18 mM (IC50) |
In Vitro
Multi-kinase-IN-4 (compound 5d) (48 h) has cytotoxic against the HepG2, MCF-7, MDA-231, and HeLa cell lines with IC50 values of 7.10, 2.48, 1.94, and 6.38 µM, respectively, but displays less cytotoxic activity on the WI38 cells (IC50 = 64.29 µM) [1].
Multi-kinase-IN-4 (7.1 µM, 24 h, HepG2 cells ) increases the cell population of 9.23% at the S phase and results in a drop in the cell population at the G0-G1 and G2/M phases: 4.50% and 4.73%, respectively[1].
Multi-kinase-IN-4 (7.1 µM, 24 h, HepG2 cells) induces early and late apoptosis as well as necrotic cell death by 7.51%, 3.45%, and 3.08%, respectively[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HepG2
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Concentration:7.1 µM
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Incubation Time:24h
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Result:Induced early and late apoptosis as well as necrotic cell death by 7.51%, 3.45%, and 3.08%, respectively.
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Cell Line:HepG2
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Concentration:7.1 µM
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Incubation Time:24h
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Result:Increased the cell population of 9.23% at the S phase and results in a drop in the cell population at the G0-G1 and G2/M phases: 4.50% and 4.73%, respectively
- EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
- Detection of Apoptosis
- TUNEL staining for apoptotic DNA fragmentation
- Flow cytometric DNA-content cell-cycle staining
- Annexin V plus membrane-impermeant dye apoptosis staining
- BrdU Incorporation Assay
- Apoptosis Solutions
- Protocol for Cell Cycle
Chemical Information
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Masse moléculaire 402.91
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Formule C21H20ClFN2OS
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SMILES
O=C1N(C/C=C(CC)\C)C(SCC2=CC=C(Cl)C=C2)=NC3=C1C=C(F)C=C3
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureté et documentation
Références
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Multi-kinase-IN-4 Related Classifications
HY-149407 Related Classifications
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