Art. -Nr.: HY-13523

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PAC-1

(Synonyms: Procaspase activating compound 1)

7 Cited Publications

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Based on 7 publication(s) in Google Scholar

PAC-1 is a procaspase-3 activator that induces apoptosis in cancer cells with an EC₅₀ of 2.08 μM.

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Reinheit: 99.88%
CAS. Nr.: 315183-21-2
Formel: C₂₃H₂₈N₄O₂
Molecular Weight:392.49

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

PAC-1 Chemical Structure

CAS. Nr.: 315183-21-2

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
- Clinical Trial
Chemical Information
Publications (7)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Protokoll
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

PAC-1 is a procaspase-3 activator that induces apoptosis in cancer cells with an EC₅₀ of 2.08 μM.

IC₅₀ & Target^[1]

Procaspase-3

2.08 μM (EC₅₀)

Alle Caspase Isoform-spezifische Produkte anzeigen :

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	2.51 μM Compound: PAC-1	Cytotoxicity against human A549 cells after 72 hrs by MTT assay Cytotoxicity against human A549 cells after 72 hrs by MTT assay	[PMID: 25874341]
B16-F10	IC₅₀	>100 μM Compound: 1, PAC-1	Activation of procaspase-3-mediated human B16-F10 cell death after 24 hrs by MTS/PMS assay Activation of procaspase-3-mediated human B16-F10 cell death after 24 hrs by MTS/PMS assay	[PMID: 16936720]
BT-549	IC₅₀	14.3 μM Compound: 19	Cytotoxicity against Smac mimetic-resistant human BT549 cells assessed as growth inhibition after 24 hrs by CellTiter-Glo assay Cytotoxicity against Smac mimetic-resistant human BT549 cells assessed as growth inhibition after 24 hrs by CellTiter-Glo assay	[PMID: 27578248]
Cancer cell lines	IC₅₀	0.003 μM Compound: 1, PAC-1	Cytotoxicity against human colon cancer cells after 24 hrs by MTS/PMS assay Cytotoxicity against human colon cancer cells after 24 hrs by MTS/PMS assay	[PMID: 16936720]
Colon cell	IC₅₀	5.02 μM Compound: 1, PAC-1	Cytotoxicity against human colon cells after 24 hrs by MTS/PMS assay Cytotoxicity against human colon cells after 24 hrs by MTS/PMS assay	[PMID: 16936720]
EL4	IC₅₀	6.5 μM Compound: 1, PAC-1	Cytotoxicity against mouse EL4 cells assessed as cell death after 72 hrs by sulforhodamine B assay Cytotoxicity against mouse EL4 cells assessed as cell death after 72 hrs by sulforhodamine B assay	[PMID: 25856364]
HL-60	IC₅₀	0.92 μM Compound: 1, PAC-1	Activation of procaspase-3-mediated human HL60 cell death after 72 hrs by MTS/PMS assay Activation of procaspase-3-mediated human HL60 cell death after 72 hrs by MTS/PMS assay	[PMID: 16936720]
HL-60	IC₅₀	0.92 μM Compound: 38; PAC-1	Antitumor activity against human HL-60 cells assessed as cell growth inhibition Antitumor activity against human HL-60 cells assessed as cell growth inhibition	[PMID: 36858050]
Hs-578T	IC₅₀	>100 μM Compound: 1, PAC-1	Activation of procaspase-3-mediated human Hs 578T cell death after 24 hrs by MTS/PMS assay Activation of procaspase-3-mediated human Hs 578T cell death after 24 hrs by MTS/PMS assay	[PMID: 16936720]
HT-29	IC₅₀	0.83 μM Compound: 7, PAC-1	Cytotoxicity against human HT-29 cells after 72 hrs by MTT assay Cytotoxicity against human HT-29 cells after 72 hrs by MTT assay	[PMID: 23838381]
HT-29	IC₅₀	0.83 μM Compound: 8; PAC-1	Cytotoxicity against human HT-29 cells after 72 hrs by MTT assay Cytotoxicity against human HT-29 cells after 72 hrs by MTT assay	[PMID: 26897090]
HT-29	IC₅₀	0.97 μM Compound: 5, PAC-1	Cytotoxicity against human HT-29 cells assessed as growth inhibition after 72 hrs by MTT assay Cytotoxicity against human HT-29 cells assessed as growth inhibition after 72 hrs by MTT assay	[PMID: 23059545]
HT-29	IC₅₀	1.02 μM Compound: PAC-1	Cytotoxicity against human HT-29 cells after 72 hrs by MTT assay Cytotoxicity against human HT-29 cells after 72 hrs by MTT assay	[PMID: 25874341]
HT-29	IC₅₀	1.36 μM Compound: PAC-1	Antiproliferative activity against human HT-29 cells after 72 hrs by MTT assay Antiproliferative activity against human HT-29 cells after 72 hrs by MTT assay	[PMID: 25171780]
Jurkat	IC₅₀	4.4 μM Compound: 1, PAC-1	Cytotoxicity against human Jurkat cells assessed as cell death after 72 hrs by sulforhodamine B assay Cytotoxicity against human Jurkat cells assessed as cell death after 72 hrs by sulforhodamine B assay	[PMID: 25856364]
KE-37	IC₅₀	241.66 nM Compound: PAC-1	Anticancer activity against human KE-37 cells assessed as reduction in cell viability Anticancer activity against human KE-37 cells assessed as reduction in cell viability	[PMID: 33774345]
MCF-10A	IC₅₀	3.2 μM Compound: 1, PAC-1	Activation of procaspase-3-mediated human MCF-10A cell death after 72 hrs by MTS/PMS assay Activation of procaspase-3-mediated human MCF-10A cell death after 72 hrs by MTS/PMS assay	[PMID: 16936720]
MCF7	IC₅₀	>40 μM Compound: PAC-1	Antiproliferative activity against human MCF7 cells after 24 hrs by CCK-8 assay Antiproliferative activity against human MCF7 cells after 24 hrs by CCK-8 assay	[PMID: 31015910]
MCF7	IC₅₀	>75 μM Compound: 1, PAC-1	Activation of procaspase-3 in human MCF-7 cells assessed as cell death after 72 hrs Activation of procaspase-3 in human MCF-7 cells assessed as cell death after 72 hrs	[PMID: 16936720]
MCF7	IC₅₀	>75 μM Compound: 38; PAC-1	Antitumor activity against human MCF7 cells assessed as cell growth inhibition Antitumor activity against human MCF7 cells assessed as cell growth inhibition	[PMID: 36858050]
MDA-MB-231	IC₅₀	3.2 μM Compound: PAC-1	Cytotoxicity against human MDA-MB-231 cells after 72 hrs by MTT assay Cytotoxicity against human MDA-MB-231 cells after 72 hrs by MTT assay	[PMID: 25874341]
MDA-MB-231	IC₅₀	4.6 μM Compound: 7, PAC-1	Cytotoxicity against human MDA-MB-231 cells after 72 hrs by MTT assay Cytotoxicity against human MDA-MB-231 cells after 72 hrs by MTT assay	[PMID: 23838381]
MDA-MB-231	IC₅₀	4.6 μM Compound: 8; PAC-1	Cytotoxicity against human MDA-MB-231 cells after 72 hrs by MTT assay Cytotoxicity against human MDA-MB-231 cells after 72 hrs by MTT assay	[PMID: 26897090]
MDA-MB-231	IC₅₀	4.7 μM Compound: 19	Cytotoxicity against Smac mimetic-sensitive human MDA-MB-231 cells assessed as growth inhibition after 24 hrs by CellTiter-Glo assay Cytotoxicity against Smac mimetic-sensitive human MDA-MB-231 cells assessed as growth inhibition after 24 hrs by CellTiter-Glo assay	[PMID: 27578248]
MDA-MB-231	IC₅₀	4.8 μM Compound: PAC-1	Antiproliferative activity against human MDA-MB-435 cells after 72 hrs by MTT assay Antiproliferative activity against human MDA-MB-435 cells after 72 hrs by MTT assay	[PMID: 25171780]
MDA-MB-231	IC₅₀	6.11 μM Compound: 5, PAC-1	Cytotoxicity against human MDA-MB-231 cells assessed as growth inhibition after 72 hrs by MTT assay Cytotoxicity against human MDA-MB-231 cells assessed as growth inhibition after 72 hrs by MTT assay	[PMID: 23059545]
MDA-MB-453	IC₅₀	2 μM Compound: 19	Cytotoxicity against Smac mimetic-resistant human MDA-MB-453 cells assessed as growth inhibition after 24 hrs by CellTiter-Glo assay Cytotoxicity against Smac mimetic-resistant human MDA-MB-453 cells assessed as growth inhibition after 24 hrs by CellTiter-Glo assay	[PMID: 27578248]
MKN-45	IC₅₀	0.82 μM Compound: 7, PAC-1	Cytotoxicity against human MKN45 cells after 72 hrs by MTT assay Cytotoxicity against human MKN45 cells after 72 hrs by MTT assay	[PMID: 23838381]
MKN-45	IC₅₀	0.82 μM Compound: 8; PAC-1	Cytotoxicity against human MKN45 cells after 72 hrs by MTT assay Cytotoxicity against human MKN45 cells after 72 hrs by MTT assay	[PMID: 26897090]
MKN-45	IC₅₀	2.61 μM Compound: PAC-1	Antiproliferative activity against human MKN45 cells after 72 hrs by MTT assay Antiproliferative activity against human MKN45 cells after 72 hrs by MTT assay	[PMID: 25171780]
NCI-H226	IC₅₀	0.35 μM Compound: 1, PAC-1	Activation of procaspase-3-mediated human NCI-H226 cell death after 24 hrs by MTS/PMS assay Activation of procaspase-3-mediated human NCI-H226 cell death after 24 hrs by MTS/PMS assay	[PMID: 16936720]
NCI-H226	IC₅₀	0.35 μM Compound: 38; PAC-1	Antitumor activity against human NCI-H226 cells assessed as cell growth inhibition Antitumor activity against human NCI-H226 cells assessed as cell growth inhibition	[PMID: 36858050]
NCI-H226	IC₅₀	1.02 μM Compound: PAC-1	Antiproliferative activity against human NCI-H226 cells after 72 hrs by MTT assay Antiproliferative activity against human NCI-H226 cells after 72 hrs by MTT assay	[PMID: 25171780]
NCI-H446	IC₅₀	2326.69 μM Compound: PAC-1	Anticancer activity against human NCI-H446 cells assessed as reduction in cell viability Anticancer activity against human NCI-H446 cells assessed as reduction in cell viability	[PMID: 33774345]
NCI-H460	IC₅₀	1.25 μM Compound: PAC-1	Cytotoxicity against human H460 cells after 72 hrs by MTT assay Cytotoxicity against human H460 cells after 72 hrs by MTT assay	[PMID: 25874341]
NCI-H460	IC₅₀	3.57 μM Compound: 5, PAC-1	Cytotoxicity against human H460 cells assessed as growth inhibition after 72 hrs by MTT assay Cytotoxicity against human H460 cells assessed as growth inhibition after 72 hrs by MTT assay	[PMID: 23059545]
PBL	IC₅₀	123.81 μM Compound: 8; PAC-1	Cytotoxicity against human PBL cells after 72 hrs by MTT assay Cytotoxicity against human PBL cells after 72 hrs by MTT assay	[PMID: 26897090]
PC-12	EC₅₀	122 μM Compound: 2, PAC-1	Induction of apoptosis in rat PC12 cells after 24 hrs by trypan blue exclusion assay Induction of apoptosis in rat PC12 cells after 24 hrs by trypan blue exclusion assay	[PMID: 23859779]
SK-N-SH	IC₅₀	3.06 μM Compound: PAC-1	Antiproliferative activity against human SK-N-SH cells after 72 hrs by MTT assay Antiproliferative activity against human SK-N-SH cells after 72 hrs by MTT assay	[PMID: 25171780]
U-937	IC₅₀	10.2 μM Compound: 1, PAC-1	Cytotoxicity against human U937 cells assessed as cell death after 72 hrs by sulforhodamine B assay Cytotoxicity against human U937 cells assessed as cell death after 72 hrs by sulforhodamine B assay	[PMID: 25856364]
U-937	IC₅₀	4.8 μM Compound: 1, PAC-1	Cytotoxicity against human U937 cells after 72 hrs by sulforhodamine B assay Cytotoxicity against human U937 cells after 72 hrs by sulforhodamine B assay	[PMID: 19708658]
U-937	IC₅₀	6 μM Compound: PAC-1	Antiproliferative activity against human procaspase-3 over-expressing U937 cells after 24 hrs by CCK-8 assay Antiproliferative activity against human procaspase-3 over-expressing U937 cells after 24 hrs by CCK-8 assay	[PMID: 31015910]
UACC-62	IC₅₀	>100 μM Compound: 1, PAC-1	Activation of procaspase-3-mediated human UACC62 cell death after 24 hrs by MTS/PMS assay Activation of procaspase-3-mediated human UACC62 cell death after 24 hrs by MTS/PMS assay	[PMID: 16936720]

In Vitro

PAC-1 activates procaspase-3 with an EC₅₀ of 2.08 μM. PAC-1 exhibits an enhanced zinc chelating ability (EC₅₀= 7.08 μM). PAC-1 induces leukemia cell death with IC₅₀ of 4.03 μM, which is consistent with the values reported by other investigators. PAC-1 treatment also results in death of other malignant cells in a concentration-dependent manner with IC₅₀s ranging from 4.03 to 53.44μM. The overall mean IC₅₀ in the fifteen malignant cell lines is 0.88 mM for WF-210 and 19.40 μM for PAC-1. In contrast, the sensitivity of the normal human cells (PBL, L-02, HUVEC and MCF 10A) to WF-210 is 2.6-fold lower (mean IC₅₀=412.34 μM) than PAC-1 (mean IC₅₀=158.29 μM)^[1]. Procaspase-activating compound-1 (PAC-1) is the first direct caspase-activating compound discovered. PAC-1 treatment upregulates Ero1α in multiple cell lines, whereas silencing of Ero1α significantly inhibits calcium release from ER and cell death^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

To evaluate the in vivo effect of WF-210 on the growth of malignant tumors, we examined the ability of WF-210 to suppress tumor growth in mouse Hep3B and MDA-MB-435 xenograft models. These two cell lines express procaspase-3 at relatively high levels. Tumors induced by xenografts of the liver cancer cell Hep3B are allowed to develop and grow to a size of 100 mm³, after which WF-210 (2.5 mg/kg) or PAC-1 (5.0 mg/kg) is given daily for two weeks by intravenous (i.v.) administration. As shown in both PAC-1 and WF-210 significantly inhibits the growth of Hep3B tumor xenografts^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS. Nr. 315183-21-2
Appearance Solid
Molecular Weight 392.49
Formel C₂₃H₂₈N₄O₂
Color White to yellow
SMILES

O=C(N/N=C/C1=CC=CC(CC=C)=C1O)CN2CCN(CC3=CC=CC=C3)CC2
Synonyms

Procaspase activating compound 1
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (7)

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Cell Mol Life Sci

Suppression of Skp2 contributes to sepsis-induced acute lung injury by enhancing ferroptosis through the ubiquitination of SLC3A2. [Abstract]

2024 Jul 30;81(1):325. PMID: 39079969
Mol Cancer Ther

Discovery of New Targets to Control Metastasis in Pancreatic Cancer by Single-cell Transcriptomics Analysis of Circulating Tumor Cells. [Abstract]

2020 Aug;19(8):1751-1760. PMID: 32499301
Cancers (Basel)

High-Dosage NMN Promotes Ferroptosis to Suppress Lung Adenocarcinoma Growth through the NAM-Mediated SIRT1-AMPK-ACC Pathway. [Abstract]

2023 Apr 23;15(9):2427. PMID: 37173894
Poult Sci

Protective effects of hypericin against infectious bronchitis virus induced apoptosis and reactive oxygen species in chicken embryo kidney cells. [Abstract]

2019 Dec 1;98(12):6367-6377. PMID: 31399732
Vet Microbiol

Lipid metabolism is a novel and practical source of potential targets for antiviral discovery against porcine parvovirus. [Abstract]

2021 Oct:261:109177. PMID: 34391196
SSRN

2025 Oct 9.
Oncotarget

RXRα ligand Z-10 induces PML-RARα cleavage and APL cell apoptosis through disrupting PML-RARα/RXRα complex in a cAMP-independent manner. [Abstract]

2017 Feb 14;8(7):12311-12322. PMID: 28129653

PAC-1 purchased from MedChemExpress. Usage Cited in: Oncotarget. 2017 Feb 14;8(7):12311-12322. [Abstract]

NB4 cells are treated with 5 μM Z-10 or 50 μM PAC-1 for the indicated time, and the expression of PML-RARα and cleaved caspase3 is analyzed by western blot.

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 50 mg/mL (127.39 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.5478 mL

12.7392 mL

25.4784 mL

5 mM

0.5096 mL

2.5478 mL

5.0957 mL

10 mM

0.2548 mL

1.2739 mL

2.5478 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (6.37 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: 2.5 mg/mL (6.37 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (6.37 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Protokoll

Kinase Assay^[1]

Various concentrations of WF-210 or PAC-1 are added to procaspase-3 in buffer containing 50 mM HEPES, 0.1% CHAPS, 10% glycerol, 100 mM NaCl, 0.1 mM EDTA, 10 mM DTT pH 7.4,and incubated for 12 h at 37°C. The final volume is 10 mL and the final concentration of procaspase-3 is 1 mM. Then 40 mL of the substrate Ac-DEVD-pNA (final concentration 0.4 mM) in buffer containing 50 mM HEPES pH 7.4, 100 mM NaCl, 10 mM DTT, 0.1 mM EDTA disodium salt, 0.10% CHAPS, 10% glycerol is added and the absorbance of the plate is read at 405 nm for a total of 1 h. The slope of the linear portion for each well is determined as the enzyme activity^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Assay^[1]

Cell viability is measured using the MTT method or the Cell Titer-Glo luminescent assay. For the MTT assay, the cells (1×10⁵ cells/mL) are seeded into 96- well culture plates. After overnight incubation, cells are treated with various concentrations of agents (PAC-1, WF-210 or other agents) for 24 or 72 h. Then 10 mL MTT solution (2.5 mg/mL in PBS) is added to each well, and the plates are incubated for an additional 4 h at 37°C. After centrifugation (2500 rpm, 10min), the medium containing MTT is aspirated, and100mL DMSO is added. The optical density of each well is measured at 570 nm with a Biotek Synergy HT Reader. The Cell Titer-Glo kit is used to determine the relative levels of intracellular ATP as a biomarker for live cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration^[1]

Mice^[1]
To determine the in vivo anti-tumor activity of WF-210, viable human gallbladder cancer GBC-SD cells (5×10⁶/100 mL PBS per mouse), human breast cancer MDA-MB-435 cells (1×10⁷/100 mL PBS per mouse), human liver cancer Hep3B cells (5×10⁶/100 mL PBS per mouse) and human breast cancer MCF-7 cells (1×10⁷/100 mL PBS per mouse) are subcutaneously (s.c.) injected into the right flank of 7- to 8-week old male SCID mice or Balb/c nude mice. Cell numbers are confirmed by trypan blue staining prior to injection. Specially, MCF-7 xenograft mice are also administered with the hormone 17-beta-estradiol (3 mg/kg) on alternate days. When the average s.c. tumor volume reached 100 mm³, mice are randomly divided into various treatment and control groups (eight mice per group). Tumor size is measured once every two days with a caliper (calculated volume=shortest diameter²×longest diameter/2). Body weight, diet consumption and tumor size are recorded once every two days. After two or four weeks, mice are sacrificed and tumors are excised and stored at -80°C until further analysis.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (277 KB)
SDS (597 KB)
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- Français - FR (597 KB)
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COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Wang F, et al. A novel small-molecule activator of procaspase-3 induces apoptosis in cancer cells and reduces tumor growth in human breast, liver and gallbladder cancer xenografts. Mol Oncol. 2014 Dec;8(8):1640-52. [Content Brief]

[2]. Seervi M, et al. ERO1α-dependent endoplasmic reticulum-mitochondrial calcium flux contributes to ER stress and mitochondrial permeabilization by procaspase-activating compound-1 (PAC-1). Cell Death Dis. 2013 Dec 19;4:e968. [Content Brief]

[3]. Putt KS, et al. Small-molecule activation of procaspase-3 to caspase-3 as a personalized anticancer strategy. Nat Chem Biol. 2006 Oct;2(10):543-50. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.5478 mL	12.7392 mL	25.4784 mL	63.6959 mL
	5 mM	0.5096 mL	2.5478 mL	5.0957 mL	12.7392 mL
	10 mM	0.2548 mL	1.2739 mL	2.5478 mL	6.3696 mL
	15 mM	0.1699 mL	0.8493 mL	1.6986 mL	4.2464 mL
	20 mM	0.1274 mL	0.6370 mL	1.2739 mL	3.1848 mL
	25 mM	0.1019 mL	0.5096 mL	1.0191 mL	2.5478 mL
	30 mM	0.0849 mL	0.4246 mL	0.8493 mL	2.1232 mL
	40 mM	0.0637 mL	0.3185 mL	0.6370 mL	1.5924 mL
	50 mM	0.0510 mL	0.2548 mL	0.5096 mL	1.2739 mL
	60 mM	0.0425 mL	0.2123 mL	0.4246 mL	1.0616 mL
	80 mM	0.0318 mL	0.1592 mL	0.3185 mL	0.7962 mL
	100 mM	0.0255 mL	0.1274 mL	0.2548 mL	0.6370 mL

Keywords

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PAC-1

Apoptosis Compound Library

Anti-Cancer Compound Library

Clinical Compound Library

Autophagy Compound Library

Drug Repurposing Compound Library

Pyroptosis Compound Library

Anti-Pancreatic Cancer Compound Library

Anti-Blood Cancer Compound Library

Targeted Diversity Library

Cysteine Targeted Covalent Library

Highly Selective Activators Library

Cell Death Library

Anti-Hematopathy Compound Library

Bioactive Compound Library Max

Drug Repurposing Compound Library Plus

Clinical Compound Library Plus

Bioactive Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) PAC-1

Alle Caspase Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (7)

Lösungsmittel & Löslichkeit

Protokoll

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

PAC-1

Apoptosis Compound Library

Anti-Cancer Compound Library

Clinical Compound Library

Autophagy Compound Library

Drug Repurposing Compound Library

Pyroptosis Compound Library

Anti-Pancreatic Cancer Compound Library

Anti-Blood Cancer Compound Library

Targeted Diversity Library

Cysteine Targeted Covalent Library

Highly Selective Activators Library

Cell Death Library

Anti-Hematopathy Compound Library

Bioactive Compound Library Max

Drug Repurposing Compound Library Plus

Clinical Compound Library Plus

Bioactive Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) PAC-1

Alle Caspase Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (7)

Lösungsmittel & Löslichkeit

Protokoll

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

PAC-1 Related Classifications

HY-13523 Related Classifications