C646
Based on 97 publication(s) in Google Scholar
C646 is a selective and competitive histone acetyltransferase p300 inhibitor with Ki of 400 nM, and is less potent for other acetyltransferases.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza: 99.88%
- No. CAS: 328968-36-1
- Fòrmula: C24H19N3O6
- Peso molecular:445.42
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Publications Citing Use of MedChemExpress (MCE) C646
More- Cell Res. 2023 Sep;33(9):679-698. [Abstract]
- Immunity. 2024 Feb 13;57(2):364-378.e9. [Abstract]
- Cancer Res. 2025 Jul 25. [Abstract]
- Adv Funct Mater. 2023 Dec 21.
- Nat Microbiol. 2021 Jul;6(7):932-945. [Abstract]
- Autophagy. 2026 Jun 12:1-18. [Abstract]
- Exp Mol Med. 2026 May;58(5):1536-1555. [Abstract]
- J Adv Res. 2025 Aug 5:S2090-1232(25)00587-9. [Abstract]
- J Adv Res. 2025 Apr:70:255-269. [Abstract]
- Neuron. 2025 Jul 23;113(14):2280-2296.e8. [Abstract]
- Mol Cell. 2025 Jul 17;85(14):2733-2748.e7. [Abstract]
- J Nanobiotechnology. 2026 Jan 29;24(1):156. [Abstract]
- Nucleic Acids Res. 2019 Mar 18;47(5):2455-2471. [Abstract]
- Theranostics. 2023 Jul 3;13(11):3856-3871. [Abstract]
- Theranostics. 2021 Jan 1;11(3):1473-1492. [Abstract]
- J Exp Clin Cancer Res. 2025 Oct 8;44(1):285. [Abstract]
- J Exp Clin Cancer Res. 2021 May 10;40(1):162. [Abstract]
- Adv Sci (Weinh). 2026 May 27:e21445. [Abstract]
- Adv Sci (Weinh). 2026 Apr;13(23):e18185. [Abstract]
- Cell Death Differ. 2018 Nov;25(11):1980-1995. [Abstract]
- Research (Wash D C). 2026 Jan 6:9:1052. [Abstract]
- Cancer Biol Med. 2020 Feb 15;17(1):88-100. [Abstract]
- Cell Death Dis. 2026 Mar 2;17(1):288. [Abstract]
- Pharmacol Res. 2025 Mar:213:107632. [Abstract]
- Pharmacol Res. 2024 Sep:207:107306. [Abstract]
- Cell Death Dis. 2022 Feb 17;13(2):159. [Abstract]
- Cancer Lett. 2024 Jul 1:593:216925. [Abstract]
- Small. 2024 Mar;20(9):e2305218. [Abstract]
- Int J Biol Sci. 2021 Jan 1;17(2):514-526. [Abstract]
- Phytomedicine. 2025 May:140:156567. [Abstract]
- Phytomedicine. 2023 Sep:118:154956. [Abstract]
- Cell Death Discov. 2026 Apr 21;12(1):266. [Abstract]
- Acta Pharmacol Sin. 2021 Nov;42(11):1834-1846. [Abstract]
- Cell Death Discov. 2021 May 31;7(1):128. [Abstract]
- Oncogene. 2025 Sep;44(34):3096-3112. [Abstract]
- Oncogene. 2021 Apr;40(15):2711-2724. [Abstract]
- Apoptosis. 2025 Dec;30(11-12):3087-3104. [Abstract]
- Cell Chem Biol. 2024 Jul 18;31(7):1363-1372.e8. [Abstract]
- Int J Biol Macromol. 2026 Apr:357:151454. [Abstract]
- Int J Biol Macromol. 2024 Nov;280(Pt 4):135580. [Abstract]
- Int J Biol Macromol. 2024 Oct;277(Pt 4):134325. [Abstract]
- Cell Mol Gastroenterol Hepatol. 2025 Sep 22:101640. [Abstract]
- Free Radic Biol Med. 2020 Dec:161:150-162. [Abstract]
- Free Radic Biol Med. 2018 Aug 20:124:454-465. [Abstract]
- J Anim Sci Biotechnol. 2025 Dec 18;16(1):174. [Abstract]
- Stem Cell Res Ther. 2025 Aug 27;16(1):462. [Abstract]
- Cell Rep. 2021 Nov 9;37(6):109988. [Abstract]
- Cell Mol Neurobiol. 2021 Aug;41(6):1257-1269. [Abstract]
- Hum Reprod. 2019 Jul 8;34(7):1186-1194. [Abstract]
- J Agric Food Chem. 2022 Jan 12;70(1):403-414. [Abstract]
- Biochem Pharmacol. 2023 Apr:210:115497. [Abstract]
- Mol Ther Nucleic Acids. 2021 Nov 3:26:1215-1227. [Abstract]
- Mol Ther Nucleic Acids. 2021 Jan 1:23:667-681. [Abstract]
- Life Sci. 2020 Oct 1:258:118243. [Abstract]
- Inflamm Res. 2023 Jul;72(7):1441-1452. [Abstract]
- Oncogenesis. 2021 Oct 27;10(10):72. [Abstract]
- Commun Biol. 2025 Nov 20;8(1):1626. [Abstract]
- Clin Epigenetics. 2021 Jun 10;13(1):127. [Abstract]
- Eur J Pharmacol. 2017 Oct 5:812:206-215. [Abstract]
- Int Immunopharmacol. 2026 Jan 15:169:116021. [Abstract]
- Int Immunopharmacol. 2025 Jan 10:144:113712. [Abstract]
- Noncoding RNA Res. 2024 Jan 10;9(2):330-340. [Abstract]
- Int J Mol Sci. 2023 Apr 11;24(8):7031. [Abstract]
- Biol Direct. 2024 Jun 21;19(1):48. [Abstract]
- Biol Direct. 2022 Nov 25;17(1):34. [Abstract]
- Immunology. 2023 Jan;168(1):49-62. [Abstract]
- Chem Biol Interact. 2022 Jan 5:351:109740. [Abstract]
- Biochim Biophys Acta Mol Basis Dis. 2026 Aug;1872(6):168281. [Abstract]
- PLoS Pathog. 2025 Jul 8;21(7):e1013331. [Abstract]
- Cancer Sci. 2025 Jun;116(6):1565-1578. [Abstract]
- Stem Cell Rev Rep. 2023 Oct;19(7):2329-2340. [Abstract]
- APL Bioeng. 2023 Feb 22;7(1):016110. [Abstract]
- Stem Cell Rev Rep. 2021 Jun;17(3):938-951. [Abstract]
- Neoplasia. 2023 Apr:38:100890. [Abstract]
- Cell Signal. 2025 Dec:136:112079. [Abstract]
- J Cell Mol Med. 2021 Aug;25(15):7524-7537. [Abstract]
- Aquaculture. 2025 Feb 15.
- J Cell Commun Signal. 2025 Jul 23;19(3):e70028. [Abstract]
- J Immunol. 2022 Aug 1;209(3):476-487. [Abstract]
- Cell Cycle. 2019 Aug;18(15):1784-1797. [Abstract]
- Toxicol Appl Pharmacol. 2026 Jun 16:514:117916. [Abstract]
- Mol Carcinog. 2023 Apr;62(4):503-516. [Abstract]
- Insect Mol Biol. 2020 Feb;29(1):104-111. [Abstract]
- Immunobiology. 2025 Apr 21;230(3):152902. [Abstract]
- Epigenomics. 2024;16(15-16):1097-1115. [Abstract]
- Am J Cancer Res. 2021 Jun 15;11(6):3021-3038. [Abstract]
- Insects. 2023 Mar 23;14(4):309. [Abstract]
- Biochem Biophys Res Commun. 2016 Dec 2;481(1-2):90-96. [Abstract]
- Arch Insect Biochem Physiol. 2021 Jul;107(3):e21823. [Abstract]
- bioRxiv. 2026 Mar 10.
- bioRxiv. 2026 Jan 23:2026.01.22.701139. [Abstract]
- bioRxiv. 2025 Dec 19.
- bioRxiv. 2025 Jul 21.
- Res Sq. 2024 Jul 15.
- Environ Toxicol. 2023 Dec;38(12):2857-2866. [Abstract]
- Research Square Preprint. 2023 Apr 11.
- Oxid Med Cell Longev. 2021 Jun 21:2021:6673343. [Abstract]
Customer Validation & Images
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Actividad biológica
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CBP/p300 |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A549 | IC50 |
10 μM
Compound: C646
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Anticlonogenic activity against human A549 cells after 10 days
Anticlonogenic activity against human A549 cells after 10 days
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[PMID: 28686912] |
| MCF7 | IC50 |
26.09 μM
Compound: 1a; C646
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Antiproliferative activity against HER2-negative human MCF7 cells measured after 6 days of incubation by sulforhodamine B assay
Antiproliferative activity against HER2-negative human MCF7 cells measured after 6 days of incubation by sulforhodamine B assay
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[PMID: 31306905] |
| NCI-H157 | IC50 |
6 μM
Compound: C646
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Anticlonogenic activity against human NCI-H157 cells after 10 days
Anticlonogenic activity against human NCI-H157 cells after 10 days
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[PMID: 28686912] |
| NCI-H460 | IC50 |
6.3 μM
Compound: C646
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Anticlonogenic activity against human NCI-H460 cells after 10 days
Anticlonogenic activity against human NCI-H460 cells after 10 days
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[PMID: 28686912] |
| T47D | IC50 |
7.8 μM
Compound: 1a; C646
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Antiproliferative activity against HER2-negative human T47D cells measured after 6 days of incubation by sulforhodamine B assay
Antiproliferative activity against HER2-negative human T47D cells measured after 6 days of incubation by sulforhodamine B assay
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[PMID: 31306905] |
C646 is a linear competitive inhibitor of p300 versus acetyl-CoA with a Ki of 400 nM. C646 shows a noncompetitive pattern of p300 inhibition versus the H4-15 peptide substrate. C646 treatment reduces histone H3 and H4 acetylation levels and abrogates TSA-induced acetylation in cells. C646 has a more potent effect on cell growth than Lys-CoA-Tat does[1]. C646 enhances mitotic catastrophe after IR and suppresses phosphorylation of CHK1 after IRin A549 cells[2]. C646 attenuates the increased acetylation of GATA1 and the increased transcriptional activity of GATA1 induced by EDAG[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Fourteen-week-old male db/db mice and normal m/m mice[4]
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Dosage:30 nmol/g
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Administration:Intraperitoneally injected; daily; 2 weeks
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Result:The db/db mice showed greater body masses and higher levels of fasting blood glucose than the m/m mice.
Chemical Information
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No. CAS 328968-36-1
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Appearance Solid
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Peso molecular 445.42
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Fòrmula C24H19N3O6
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Color Brown to reddish brown
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SMILES
O=C(O)C1=CC=C(N2N=C(C)/C(C2=O)=C\C3=CC=C(C4=CC(C)=C(C)C=C4[N+]([O-])=O)O3)C=C1
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (97)
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Journal Impact Factor
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Most Recent
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Cell Res
α-myosin heavy chain lactylation maintains sarcomeric structure and function and alleviates the development of heart failure. [Abstract]2023 Sep;33(9):679-698. PMID: 37443257 -
Immunity
Small-molecule CBP/p300 histone acetyltransferase inhibition mobilizes leukocytes from the bone marrow via the endocrine stress response. [Abstract]2024 Feb 13;57(2):364-378.e9. PMID: 38301651 -
Cancer Res
Mitochondrial Calcium Uniporter Links Acetyl-CoA Metabolism and H3K27 Acetylation to Maintain Glioblastoma Stem Cells. [Abstract]2025 Jul 25. PMID: 40712058 -
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Nat Microbiol
IFI16 directly senses viral RNA and enhances RIG-I transcription and activation to restrict influenza virus infection. [Abstract]2021 Jul;6(7):932-945. PMID: 33986530 -
Autophagy
H3K18la- driven neutrophil secretory autophagy promotes pulmonary endothelial dysfunction in sepsis-induced lung injury. [Abstract]2026 Jun 12:1-18. PMID: 42283498 -
Exp Mol Med
Hepatocyte estrogen-related receptor α modulates a gluconeogenic-epigenetic crosstalk counteracting MASLD/MASH progression. [Abstract]2026 May;58(5):1536-1555. PMID: 42104013 -
J Adv Res
Elevated lactate production exacerbates PM2.5-induced pulmonary fibrosis by stabilizing TGF-β1. [Abstract]2025 Aug 5:S2090-1232(25)00587-9. PMID: 40759348 -
J Adv Res
Sodium-glucose exchanger 2 inhibitor canagliflozin promotes mitochondrial metabolism and alleviates salt-induced cardiac hypertrophy via preserving SIRT3 expression. [Abstract]2025 Apr:70:255-269. PMID: 38744404 -
Neuron
Metabolic reprogramming through histone lactylation in microglia and macrophages recruits CD8+ T lymphocytes and aggravates spinal cord injury. [Abstract]2025 Jul 23;113(14):2280-2296.e8. PMID: 40328251 -
Mol Cell
YTHDC1 lactylation regulates its phase separation to enhance target mRNA stability and promote RCC progression. [Abstract]2025 Jul 17;85(14):2733-2748.e7. PMID: 40680722 -
J Nanobiotechnology
CD8a antibody-functionalized biomimetic red blood cell membrane ectosomes delivering C646 reverse CD8⁺ T Cell exhaustion via H3K18la histone delactylation in gastric cardia adenocarcinoma. [Abstract]2026 Jan 29;24(1):156. PMID: 41612336 -
Nucleic Acids Res
Dysregulation of the cohesin subunit RAD21 by Hepatitis C virus mediates host-virus interactions. [Abstract]2019 Mar 18;47(5):2455-2471. PMID: 30698808 -
Theranostics
Hepatocyte HSPA12A inhibits macrophage chemotaxis and activation to attenuate liver ischemia/reperfusion injury via suppressing glycolysis-mediated HMGB1 lactylation and secretion of hepatocytes. [Abstract]2023 Jul 3;13(11):3856-3871. PMID: 37441587 -
Theranostics
H3K27 acetylation activated-COL6A1 promotes osteosarcoma lung metastasis by repressing STAT1 and activating pulmonary cancer-associated fibroblasts. [Abstract]2021 Jan 1;11(3):1473-1492. PMID: 33391546 -
J Exp Clin Cancer Res
SENP1 drives glycolysis and cisplatin resistance in gastric cancer via desumoylating ENO1. [Abstract]2025 Oct 8;44(1):285. PMID: 41063235 -
J Exp Clin Cancer Res
Activation of the clock gene TIMELESS by H3k27 acetylation promotes colorectal cancer tumorigenesis by binding to Myosin-9. [Abstract]2021 May 10;40(1):162. PMID: 33971927 -
Adv Sci (Weinh)
β-Elemene Rescues Radiation-Induced Enteritis by Orchestrating a Host-Microbiome Circuit That Fuels Epigenetic DNA Repair. [Abstract]2026 May 27:e21445. PMID: 42201651 -
Adv Sci (Weinh)
Identification of A p300-SP1-BRD4 Transcriptional Axis as a Key Driver of AR Hyperactivation in Polycystic Ovarian Syndrome. [Abstract]2026 Apr;13(23):e18185. PMID: 41684293 -
Cell Death Differ
LncRNA CASC9 promotes esophageal squamous cell carcinoma metastasis through upregulating LAMC2 expression by interacting with the CREB-binding protein. [Abstract]2018 Nov;25(11):1980-1995. PMID: 29511340
C646 purchased from MedChemExpress. Usage Cited in: Cell Death Differ. 2018 Nov;25(11):1980-1995. [Abstract]
Western blot analysis of LAMC2 expression after C646 treatment in KYSE150 and KYSE450. C646 treatment: 20μM, 12h.
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Research (Wash D C)
Vascular Smooth Muscle Cell-Specific BCAT2 Deficiency Attenuates Diabetic Atherosclerotic Calcification via Histone Propionylation. [Abstract]2026 Jan 6:9:1052. PMID: 41503231 -
Cancer Biol Med
27-Hydroxycholesterol-induced EndMT acts via STAT3 signaling to promote breast cancer cell migration by altering the tumor microenvironment. [Abstract]2020 Feb 15;17(1):88-100. PMID: 32296578 -
Cell Death Dis
A Lamp2a-linked RNA secreted by ADSCs prevents ENO1-lactylation-glycolysis feedback and cell malignant behavior in triple-negative breast cancer. [Abstract]2026 Mar 2;17(1):288. PMID: 41771848 -
Pharmacol Res
Histone lysine crotonylation accelerates ACSL4-mediated ferroptosis of keratinocytes via modulating autophagy in diabetic wound healing. [Abstract]2025 Mar:213:107632. PMID: 39892437 -
Pharmacol Res
Apabetalone, a BET protein inhibitor, inhibits kidney damage in diabetes by preventing pyroptosis via modulating the P300/H3K27ac/PLK1 axis. [Abstract]2024 Sep:207:107306. PMID: 39002871 -
Cell Death Dis
Nanog mediated by FAO/ACLY signaling induces cellular dormancy in colorectal cancer cells. [Abstract]2022 Feb 17;13(2):159. PMID: 35177584 -
Cancer Lett
CPSF3 regulates alternative polyadenylation of CNIH2 to promote esophageal squamous cell carcinoma progression. [Abstract]2024 Jul 1:593:216925. PMID: 38718887 -
Small
Matrix Nonlinear Viscoelasticity Regulates Skeletal Myogenesis through MRTF Nuclear Localization and Nuclear Mechanotransduction. [Abstract]2024 Mar;20(9):e2305218. PMID: 37847903 -
Int J Biol Sci
KIF11 promotes cell proliferation via ERBB2/PI3K/AKT signaling pathway in gallbladder cancer. [Abstract]2021 Jan 1;17(2):514-526. PMID: 33613109 -
Phytomedicine
Xiaochaihutang ameliorates depression-like behaviors induced via chronic social defeat stress by regulating exon-specific Bdnf transcription through H3K18 acetylation in the hippocampus of mice. [Abstract]2025 May:140:156567. PMID: 40068295 -
Phytomedicine
2023 Sep:118:154956. PMID: 37499345 -
Cell Death Discov
BRD4 Inhibition alleviates sepsis-associated acute kidney injury via suppression of NOX4-mediated oxidative stress and inflammation. [Abstract]2026 Apr 21;12(1):266. PMID: 42014688 -
Acta Pharmacol Sin
SL010110, a lead compound, inhibits gluconeogenesis via SIRT2-p300-mediated PEPCK1 degradation and improves glucose homeostasis in diabetic mice. [Abstract]2021 Nov;42(11):1834-1846. PMID: 33574568 -
Cell Death Discov
P300/HDAC1 regulates the acetylation/deacetylation and autophagic activities of LC3/Atg8-PE ubiquitin-like system. [Abstract]2021 May 31;7(1):128. PMID: 34059632 -
Oncogene
ACSL5 regulated acetyl-CoA to promote bladder cancer cellular senescence via 53BP1 acetylation. [Abstract]2025 Sep;44(34):3096-3112. PMID: 40595416 -
Oncogene
2021 Apr;40(15):2711-2724. PMID: 33712705 -
Apoptosis
Mechanism of the enterobacterial metabolite sodium butyrate mediating ferroptosis to affect osteogenic ability of BMSCs in mice with estrogen deficiency-caused osteoporosis via the PTEN/PI3K/AKT pathway. [Abstract]2025 Dec;30(11-12):3087-3104. PMID: 41205030 -
Cell Chem Biol
2024 Jul 18;31(7):1363-1372.e8. PMID: 38917791 -
Int J Biol Macromol
Emodin exerts antitumor effects in cervical cancer cells by reprogramming phospholipid metabolism through modulation of H3K27ac and H3K27me3. [Abstract]2026 Apr:357:151454. PMID: 41856179 -
Int J Biol Macromol
2024 Nov;280(Pt 4):135580. PMID: 39322166 -
Int J Biol Macromol
2024 Oct;277(Pt 4):134325. PMID: 39089561 -
Cell Mol Gastroenterol Hepatol
EP300/YAP1-SERPINE1 Signaling Regulates Ductular Reaction and Liver Fibrosis in Biliary Atresia. [Abstract]2025 Sep 22:101640. PMID: 40992734 -
Free Radic Biol Med
miR-15a-5p inhibits metastasis and lipid metabolism by suppressing histone acetylation in lung cancer. [Abstract]2020 Dec:161:150-162. PMID: 33059020 -
Free Radic Biol Med
Sodium butyrate attenuates diabetes-induced aortic endothelial dysfunction via P300-mediated transcriptional activation of Nrf2. [Abstract]2018 Aug 20:124:454-465. PMID: 29964168
C646 purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2018 Aug 20:124:454-465. [Abstract]
To explore whether P300 is required for NaB’s activation of Nrf2 expression and function, HG-treated endothelial cells (ECs) are co-treated with NaB, in the presence or absence of C646.
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J Anim Sci Biotechnol
Glutamine relieves feed restriction-induced ruminal epithelial function damage through histone lysine lactylation in yaks. [Abstract]2025 Dec 18;16(1):174. PMID: 41408320 -
Stem Cell Res Ther
Extracellular lactate improves neurogenesis by modulating H3K9 lactylation and SnoN expression under hypoxic conditions. [Abstract]2025 Aug 27;16(1):462. PMID: 40866997 -
Cell Rep
Interplay between protein acetylation and ubiquitination controls MCL1 protein stability. [Abstract]2021 Nov 9;37(6):109988. PMID: 34758305 -
Cell Mol Neurobiol
Valproate-Induced Epigenetic Upregulation of Hypothalamic Fto Expression Potentially Linked with Weight Gain. [Abstract]2021 Aug;41(6):1257-1269. PMID: 32500354 -
Hum Reprod
2019 Jul 8;34(7):1186-1194. PMID: 31194865 -
J Agric Food Chem
Antimicrobial Peptide BCp12 Inhibits Staphylococcus aureus Growth by Altering Lysine Malonylation Levels in the Arginine Synthesis Pathway. [Abstract]2022 Jan 12;70(1):403-414. PMID: 34942069 -
Biochem Pharmacol
BRD4 promotes hepatic stellate cells activation and hepatic fibrosis via mediating P300/H3K27ac/PLK1 axis. [Abstract]2023 Apr:210:115497. PMID: 36907496
C646 purchased from MedChemExpress. Usage Cited in: Biochem Pharmacol. 2023 Apr:210:115497. [Abstract]
C646 (20 μM) decreases the expression of PLK1 following effective inhibition of H3K27ac, and αSMA and COLI are also repressed, in TGFβ-activated LX2 cells.
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Mol Ther Nucleic Acids
2021 Nov 3:26:1215-1227. PMID: 34853721 -
Mol Ther Nucleic Acids
Comprehensive landscape of epigenetic-dysregulated lncRNAs reveals a profound role of enhancers in carcinogenesis in BC subtypes. [Abstract]2021 Jan 1:23:667-681. PMID: 33575113 -
Life Sci
Type 2 diabetes-induced overactivation of P300 contributes to skeletal muscle atrophy by inhibiting autophagic flux. [Abstract]2020 Oct 1:258:118243. PMID: 32791154 -
Inflamm Res
Loss of TRIM24 promotes IL-10 expression via CBP/p300-dependent IFNβ1 transcription during macrophage activation. [Abstract]2023 Jul;72(7):1441-1452. PMID: 37326695 -
Oncogenesis
2021 Oct 27;10(10):72. PMID: 34707087 -
Commun Biol
Deoxynivalenol induces ferroptosis via inhibiting glycolysis-H3K18la-STEAP3 axis to promote ovary damage in piglets. [Abstract]2025 Nov 20;8(1):1626. PMID: 41266595 -
Clin Epigenetics
STAT1 epigenetically regulates LCP2 and TNFAIP2 by recruiting EP300 to contribute to the pathogenesis of inflammatory bowel disease. [Abstract]2021 Jun 10;13(1):127. PMID: 34112215 -
Eur J Pharmacol
Selective p300 inhibitor C646 inhibited HPV E6-E7 genes, altered glucose metabolism and induced apoptosis in cervical cancer cells. [Abstract]2017 Oct 5:812:206-215. PMID: 28619596
C646 purchased from MedChemExpress. Usage Cited in: Eur J Pharmacol. 2017 Oct 5:812:206-215. [Abstract]
C646 inhibits the proliferation of Cervical cancer cells and induced the accumulation of p53 protein. Protein levels of p53 and p21 are augmented by C646 in a dose-dependent manner. Whole cell lysates are analyzed with western-blot analysis.
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Int Immunopharmacol
2026 Jan 15:169:116021. PMID: 41389664 -
Int Immunopharmacol
Unveiling the interplay between hepatocyte SATB1 and innate immunity in autoimmune hepatitis. [Abstract]2025 Jan 10:144:113712. PMID: 39626541 -
Noncoding RNA Res
Exosomal MALAT1 promotes the proliferation of esophageal squamous cell carcinoma through glyoxalase 1-dependent methylglyoxal removal. [Abstract]2024 Jan 10;9(2):330-340. PMID: 38505306 -
Int J Mol Sci
2023 Apr 11;24(8):7031. PMID: 37108194 -
Biol Direct
PNSC928, a plant-derived compound, specifically disrupts CtBP2-p300 interaction and reduces inflammation in mice with acute respiratory distress syndrome. [Abstract]2024 Jun 21;19(1):48. PMID: 38902802 -
Biol Direct
CXCL1 promotes colon cancer progression through activation of NF-κB/P300 signaling pathway. [Abstract]2022 Nov 25;17(1):34. PMID: 36434686 -
Immunology
T-cell immunoglobulin- and mucin-domain-containing molecule-4 maintains adipose tissue homeostasis by orchestrating M2 macrophage polarization via nuclear factor kappa B pathway. [Abstract]2023 Jan;168(1):49-62. PMID: 35908188 -
Chem Biol Interact
The role of regulatory T cells on the activation of astrocytes in the brain of high-fat diet mice following lead exposure. [Abstract]2022 Jan 5:351:109740. PMID: 34742682 -
Biochim Biophys Acta Mol Basis Dis
Targeted inhibition of the H3K27ac/OGT/O-GlcNAcylation axis to overcome gemcitabine-induced chemotherapy resistance in bladder cancer. [Abstract]2026 Aug;1872(6):168281. PMID: 42069092 -
PLoS Pathog
Baculovirus enhances arginine uptake and induces mitochondrial autophagy to promote viral proliferation. [Abstract]2025 Jul 8;21(7):e1013331. PMID: 40627780 -
Cancer Sci
Lnc-TPT1-AS1/CBP/ATIC Axis Mediated Purine Metabolism Activation Promotes Breast Cancer Progression. [Abstract]2025 Jun;116(6):1565-1578. PMID: 40091780 -
Stem Cell Rev Rep
Metformin Promotes Proliferation of Mouse Female Germline Stem Cells by Histone Acetylation Modification of Traf2. [Abstract]2023 Oct;19(7):2329-2340. PMID: 37354386 -
APL Bioeng
Extended preconditioning on soft matrices directs human mesenchymal stem cell fate via YAP transcriptional activity and chromatin organization. [Abstract]2023 Feb 22;7(1):016110. PMID: 36845904 -
Stem Cell Rev Rep
Ubiquitin-Specific-Processing Protease 7 Regulates Female Germline Stem Cell Self-Renewal Through DNA Methylation. [Abstract]2021 Jun;17(3):938-951. PMID: 33151468 -
Neoplasia
Pharmacological inhibition of the SKP2/p300 signaling axis restricts castration-resistant prostate cancer. [Abstract]2023 Apr:38:100890. PMID: 36871351 -
Cell Signal
LOH12CR2 activated by sodium butyrate suppresses tumorigenesis in colorectal cancer via METTL14-mediated N6-methyladenosine modification of SPC24 mRNA. [Abstract]2025 Dec:136:112079. PMID: 40848939 -
J Cell Mol Med
The regulatory effect of acetylation of HMGN2 and H3K27 on pyocyanin-induced autophagy in macrophages by affecting Ulk1 transcription. [Abstract]2021 Aug;25(15):7524-7537. PMID: 34278675 -
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J Cell Commun Signal
Y-box binding protein 1 stabilizes EP300 mRNA and promotes forkhead box C1 H3K27Ac to aggravate chondrocyte injury in osteoarthritis. [Abstract]2025 Jul 23;19(3):e70028. PMID: 40703976 -
J Immunol
Deacetylation of K481 and K484 on Penaeid Shrimp Hemocyanin Is Critical for Antibacterial Activity. [Abstract]2022 Aug 1;209(3):476-487. PMID: 35851542 -
Cell Cycle
2019 Aug;18(15):1784-1797. PMID: 31223053 -
Toxicol Appl Pharmacol
Advanced glycation end products promote diabetic retinopathy through the p65-p300/SMAD4-miR-409-5p-HIF-1α-VEGF signaling pathway. [Abstract]2026 Jun 16:514:117916. PMID: 42303051 -
Mol Carcinog
Insulin-like growth factor-2 mRNA-binding protein 3 promotes cell migration, invasion, and epithelial-mesenchymal transition of esophageal squamous cell carcinoma cells by targeting zinc finger E-box-binding homeobox 1 mRNA. [Abstract]2023 Apr;62(4):503-516. PMID: 36688673 -
Insect Mol Biol
The effect of acetylation on the protein stability of BmApoLp-III in the silkworm, Bombyx mori. [Abstract]2020 Feb;29(1):104-111. PMID: 31390480 -
Immunobiology
Ureaplasma urealyticum GrpE protein elicits glycolysis-mediated inflammatory responses through TLR2 in macrophages. [Abstract]2025 Apr 21;230(3):152902. PMID: 40273504 -
Epigenomics
LINC01232 promotes ARNTL2 transcriptional activation and inhibits ferroptosis of CRC cells through p300/H3K27ac. [Abstract]2024;16(15-16):1097-1115. PMID: 39268727 -
Am J Cancer Res
Epigenetic heterogeneity promotes acquired resistance to BET bromodomain inhibition in ovarian cancer. [Abstract]2021 Jun 15;11(6):3021-3038. PMID: 34249442 -
Insects
BmCBP Catalyzes the Acetylation of BmApoLp-II Protein and Regulates Its Stability in Silkworm, Bombyx mori. [Abstract]2023 Mar 23;14(4):309. PMID: 37103124 -
Biochem Biophys Res Commun
Dimethyl α-ketoglutarate reduces CCl4-induced liver fibrosis through inhibition of autophagy in hepatic stellate cells. [Abstract]2016 Dec 2;481(1-2):90-96. PMID: 27823933
C646 purchased from MedChemExpress. Usage Cited in: Biochem Biophys Res Commun. 2016 Dec 2;481(1-2):90-96. [Abstract]
DMKG inhibits autophagy of HSCs via acetyl-coenzyme A and EP300. Western blot analysis of LC3B, Beclin-1, a-SMA, and collagen-I expression from lysates of HSC-T6 incubated with control media, DMKG (4 mM), or Lipoic acid (5 mM) in the presence or absence of C646 (10 mM) for 12 h.
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Arch Insect Biochem Physiol
The acetylation modification regulates the stability of Bm30K-15 protein and its mechanism in silkworm, Bombyx mori. [Abstract]2021 Jul;107(3):e21823. PMID: 34075635 -
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bioRxiv
2026 Jan 23:2026.01.22.701139. PMID: 41648188 -
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Environ Toxicol
H3K27 acetylation activated-CD109 evokes 5-fluorouracil resistance in gastric cancer via the JNK/MAPK signaling pathway. [Abstract]2023 Dec;38(12):2857-2866. PMID: 37661780 -
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Oxid Med Cell Longev
Resveratrol Alleviates 27-Hydroxycholesterol-Induced Senescence in Nerve Cells and Affects Zebrafish Locomotor Behavior via Activation of SIRT1-Mediated STAT3 Signaling. [Abstract]2021 Jun 21:2021:6673343. PMID: 34239694
Solvente y solubilidad
DMSO : 13.89 mg/mL (31.18 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 1.67 mg/mL (3.75 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 1.67 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 1.67 mg/mL (3.75 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 1.67 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (276 KB)
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SDS (597 KB)
- English - EN (597 KB)
- Français - FR (597 KB)
- Deutsch - DE (597 KB)
- Norwegian - NO (597 KB)
- Español - ES (597 KB)
- Swedish - SV (597 KB)
- Italian - IT (597 KB)
- Korean - KR (597 KB)
- Portuguese - PT (597 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Bowers EM, et al. Virtual ligand screening of the p300/CBP histone acetyltransferase: identification of a selective small molecule inhibitor. Chem Biol. 2010 May 28;17(5):471-82. [Content Brief]
[2]. Oike T, et al. C646, a selective small molecule inhibitor of histone acetyltransferase p300, radiosensitizes lung cancer cells by enhancing mitotic catastrophe. Radiother Oncol. 2014 May;111(2):222-7. [Content Brief]
[3]. Zheng WW, et al. EDAG positively regulates erythroid differentiation and modifies GATA1 acetylation through recruiting p300. Stem Cells. 2014 Aug;32(8):2278-89. [Content Brief]
[4]. Zhen Fan, et al. Type 2 diabetes-induced overactivation of P300 contributes to skeletal muscle atrophy by inhibiting autophagic flux. Life Sci. 2020 Aug 10;258:118243. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.2451 mL | 11.2254 mL | 22.4507 mL | 56.1268 mL |
| 5 mM | 0.4490 mL | 2.2451 mL | 4.4901 mL | 11.2254 mL | |
| 10 mM | 0.2245 mL | 1.1225 mL | 2.2451 mL | 5.6127 mL | |
| 15 mM | 0.1497 mL | 0.7484 mL | 1.4967 mL | 3.7418 mL | |
| 20 mM | 0.1123 mL | 0.5613 mL | 1.1225 mL | 2.8063 mL | |
| 25 mM | 0.0898 mL | 0.4490 mL | 0.8980 mL | 2.2451 mL | |
| 30 mM | 0.0748 mL | 0.3742 mL | 0.7484 mL | 1.8709 mL |
C646 Related Classifications
HY-13823 Related Classifications
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