Tomatidine
Based on 10 publication(s) in Google Scholar
Tomatidine acts as an anti-inflammatory agent by blocking NF-κB and JNK signaling. Tomatidine activates autophagy either in mammal cells or C elegans.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza : 98.85%
- No. CAS: 77-59-8
- Fòrmula: C27H45NO2
- Peso molecular:415.65
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Protocolo
- Pureza y Documentación
- Referencias
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) Tomatidine
More- Drug Des Devel Ther. 2024 Dec 27:18:6313-6329. [Abstract]
- Eur J Pharmacol. 2020 Sep 5:882:173280. [Abstract]
- Sci Rep. 2026 May 8;16(1):21171. [Abstract]
- PLoS Pathog. 2025 Jan 2;21(1):e1012800. [Abstract]
- J Neurochem. 2025 May;169(5):e70087. [Abstract]
- iScience. 2023 Jul 17;26(8):107408. [Abstract]
- FASEB J. 2019 Feb;33(2):2574-2586. [Abstract]
- Res Sq. 2025 Jul 13.
- Research Square Preprint. 2023 Apr 27.
- Aging (Albany NY). 2020 Jul 5;12(13):12799-12811. [Abstract]
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Ver todos los productos específicos de isoformas Endogenous Metabolite
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Actividad biológica
Descripciòn
IC50 & Target
[1]|
p65 |
JNK |
In Vitro
Tomatidine decreases inducible NO synthase and COX-2 expression through suppression of I-κBα phosphorylation, NF-κB nuclear translocation and JNK activation, which in turn inhibits c-jun phosphorylation and Oct-2 expression. Tomatidine, solasodine and diosgenin (40 μM) show 66%, 22% and 41% inhibition of nitrite production, respectively. The iNOS protein is barely detectable in unstimulated cells but markedly increases after LPS treatment, and Tomatidine causes dose-dependent inhibition of LPS-induced iNOS expression. p65 is the major component of NF-κB in LPS-stimulated macrophages, the effect of Tomatidine on p65 DNA-binding activity is determined. In the presence of Tomatidine at 10-40 μM, the binding activity of NF-κB is suppressed in a dose-dependent manner. Tomatidine inhibits the phosphorylation of I-κB, blocks the I-κB production, and furthermore suppresses p65 NF-κB translocation to the nucleus and modulated binding activity[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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No. CAS 77-59-8
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Appearance Solid
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Peso molecular 415.65
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Fòrmula C27H45NO2
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Color White to off-white
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SMILES
[H][C@]1(O[C@]2(NC[C@@H](C)CC2)[C@H]3C)C[C@@]4([H])[C@]5([H])CC[C@@]6([H])C[C@@H](O)CC[C@]6(C)[C@@]5([H])CC[C@]4(C)[C@]13[H]
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Structure Classification
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Initial Source
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (10)
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Journal Impact Factor
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Most Recent
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Drug Des Devel Ther
Tomatidine Alleviates Intervertebral Disc Degeneration by Activating the Nrf2/HO-1/GPX4 Signaling Pathway. [Abstract]2024 Dec 27:18:6313-6329. PMID: 39741916
Tomatidine purchased from MedChemExpress. Usage Cited in: Drug Des Devel Ther. 2024 Dec 27:18:6313-6329. [Abstract]
Cell counting Kit-8 assay results of NPCs stimulated with Tomatidine (Td) at different concentrations (0,1,2,4,8,16,32,64,128,256) μM at 24 and 48 h.
Tomatidine purchased from MedChemExpress. Usage Cited in: Drug Des Devel Ther. 2024 Dec 27:18:6313-6329. [Abstract]
The gene expression ADAMTS-4, Col-2 and MMP3 were detected by qPCR in the NPCs treated with Tomatidine (Td) (1, 2, 4 μM) for 24 h.
Tomatidine purchased from MedChemExpress. Usage Cited in: Drug Des Devel Ther. 2024 Dec 27:18:6313-6329. [Abstract]
The intracellular ROS levels in NPCs were measured by flow cytometry assay treated with Tomatidine (Td) (1, 2, 4 μM).
Tomatidine purchased from MedChemExpress. Usage Cited in: Drug Des Devel Ther. 2024 Dec 27:18:6313-6329. [Abstract]
The protein expression of TFR was evaluated by cell immunofluorescence treated with Tomatidine (Td) (1, 2, 4 μM).
Tomatidine purchased from MedChemExpress. Usage Cited in: Drug Des Devel Ther. 2024 Dec 27:18:6313-6329. [Abstract]
The levels of GSH and MDA in NPCs were determined by ELISA treated with Tomatidine (Td) (1, 2, 4 μM).
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Eur J Pharmacol
2020 Sep 5:882:173280. PMID: 32580039 -
Sci Rep
Tomatidine modulatesfibrosis-related markers by promoting autophagy in hepatic stellate cells via the ERK/MAPK-mTOR-ULK1 signaling pathway. [Abstract]2026 May 8;16(1):21171. PMID: 42103827 -
PLoS Pathog
2025 Jan 2;21(1):e1012800. PMID: 39746094 -
J Neurochem
Tomatidine Ameliorates Diabetes-Induced Cognitive Impairment and Tau Hyperphosphorylation Through the AMPK-TFEB Pathway. [Abstract]2025 May;169(5):e70087. PMID: 40387453 -
iScience
Tomatidine targets ATF4-dependent signaling and induces ferroptosis to limit pancreatic cancer progression. [Abstract]2023 Jul 17;26(8):107408. PMID: 37554459 -
FASEB J
Tomatidine suppresses osteoclastogenesis and mitigates estrogen deficiency-induced bone mass loss by modulating TRAF6-mediated signaling. [Abstract]2019 Feb;33(2):2574-2586. PMID: 30285579
Tomatidine purchased from MedChemExpress. Usage Cited in: FASEB J. 2019 Feb;33(2):2574-2586. [Abstract]
Tomatidine attenuates osteoclastogenesis by inhibiting the MAPK, NF-kB, and PI3K/Akt signaling pathways in vitro.
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Aging (Albany NY)
Tomatidine suppresses inflammation in primary articular chondrocytes and attenuates cartilage degradation in osteoarthritic rats. [Abstract]2020 Jul 5;12(13):12799-12811. PMID: 32628132
Solvente y solubilidad
In Vitro:
DMSO : 2 mg/mL (4.81 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
0.1 M HCl : < 1 mg/mL (insoluble)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 0.29 mg/mL (0.70 mM); Clear solution
This protocol yields a clear solution of ≥ 0.29 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (2.9 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 0.29 mg/mL (0.70 mM); Clear solution
This protocol yields a clear solution of ≥ 0.29 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (2.9 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 50% PEG300 50% Saline
Solubility: 5 mg/mL (12.03 mM); Suspended solution; Need ultrasonic
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocolo
Cell Assay[1]
RAW 264.7 cells, derived from murine macrophages, are cultured in DMEM supplemented with 10% endotoxin-free, heat-inactivated fetal calf serum, Penicillin (100 units/mL), and Streptomycin (100 μg/mL) in a 5% CO2 atmosphere at 37°C in a humidified incubator. For all assay, cell is plated at 2×105 cells/cm2 in culture dishes or plates. Treatment with vehicle (0.1% DMSO or 0.1% ethanol), test compounds and/or LPS is carried out under serum-free conditions[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Pureza y Documentación
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Ficha de datos (278 KB)
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SDS (565 KB)
- English - EN (565 KB)
- Français - FR (565 KB)
- Deutsch - DE (565 KB)
- Norwegian - NO (565 KB)
- Español - ES (565 KB)
- Swedish - SV (565 KB)
- Italian - IT (565 KB)
- Korean - KR (565 KB)
- Portuguese - PT (565 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Chiu FL, et al. Tomatidine inhibits iNOS and COX-2 through suppression of NF-kappaB and JNK pathways in LPS-stimulated mouse macrophages. FEBS Lett. 2008 Jul 9;582(16):2407-12. [Content Brief]
[2]. Anil Ahsan, et al. Tomatidine Protects Against Ischemic Neuronal Injury by Improving Lysosomal Function. Eur J Pharmacol. 2020 Jun 21;173280. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.4059 mL | 12.0294 mL | 24.0587 mL | 60.1468 mL |
Tomatidine Related Classifications
HY-N2149 Related Classifications
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