Bafilomycin C1
Based on 1 publication(s) in Google Scholar
Bafilomycin C1 is a macrolide antibiotic isolated from Streptomyces sp. Bafilomycin C1 is a potent, specific and reversible inhibitor of vacuolar-type H+-ATPases (V-ATPases). Bafilomycin C1 inhibits growth of gram-positive bacteria and fungi. Bafilomycin C1 induces cell apoptosis and can be used for the study of hepatocellular carcinoma (HCC).
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza : 99.9%
- No. CAS: 88979-61-7
- Fòrmula: C39H60O12
- Peso molecular:720.89
-
Almacenamiento:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Actividad biológica
-
Chemical Information
-
Solvente y solubilidad
- Protocolo
- Pureza y Documentación
- Referencias
-
Help & FAQs
-
HY-13259
MG-132Proteasome Inhibitor -
HY-13821
EpoxomicinProteasome Inhibitor -
HY-N6782
OligomycinH+-ATP-Synthase Inhibitor -
HY-108970
Bafilomycin DV-ATPase Inhibitor -
HY-N0877
BufalinNa+/K+ ATPase Inhibitor -
HY-15718A
Istaroxime hydrochlorideCalcium Channel Activator -
HY-13433
ThapsigarginER Stress Inducer -
HY-16589
Oligomycin AMitochondrial ATP Synthase Inhibitor -
HY-N6771
Cyclopiazonic acidECAs Inhibitor -
HY-114252
StrophanthidinCardiac Glycoside -
HY-Y0413
Biacetyl monoximeATPase Inhibitor
Publications Citing Use of MedChemExpress (MCE) Bafilomycin C1
MoreVer todos los productos específicos de isoformas Antibiotic
More
Actividad biológica
Descripciòn
IC50 & Target
|
Macrolide |
Cellular Effect
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| BGC-823 | IC50 |
1.83 μM
Compound: 7
|
Cytotoxicity against human BGC823 cells assessed as reduction in cell viability by MTT assay
Cytotoxicity against human BGC823 cells assessed as reduction in cell viability by MTT assay
|
[PMID: 26933756] |
| Caco-2 | IC50 |
1.89 μM
Compound: 7
|
Cytotoxicity against human Caco2 cells assessed as reduction in cell viability by MTT assay
Cytotoxicity against human Caco2 cells assessed as reduction in cell viability by MTT assay
|
[PMID: 26933756] |
| NCI-H460 | IC50 |
3.43 μM
Compound: 7
|
Cytotoxicity against human NCI-H460 cells assessed as reduction in cell viability by MTT assay
Cytotoxicity against human NCI-H460 cells assessed as reduction in cell viability by MTT assay
|
[PMID: 26933756] |
| SMMC-7721 | IC50 |
0.54 μM
Compound: 7
|
Cytotoxicity against human SMMC7721 cells assessed as reduction in cell viability by MTT assay
Cytotoxicity against human SMMC7721 cells assessed as reduction in cell viability by MTT assay
|
[PMID: 26933756] |
In Vitro
Bafilomycin C1 (0.33-10 μM; 6 days) inhibits the growth and proliferation of SMMC7721 and HepG2 cells in a timeand dose-dependent manner[2].Bafilomycin C1 (0.33-3.3 μM; 24 hours) decreases cyclin D3, cyclin E1, CDK2, CDK4, and CDK6 expression in both mRNA and protein expression in SMMC7721 cells[2].Bafilomycin C1 (3.3-10 μM; 24 hours) causes morphological alterations and increases the population of apoptotic cells by Hoechst 33258 (HY-15558) staining compared to vehicle[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:SMMC7721 cell and HepG2 cell
-
Concentration:0.33 μM, 1.1 μM, and 3.3 μM for SMMC77211.1 μM, 3.3 μM, and 10.0 μM for HepG2
-
Incubation Time:6 days
-
Result:Retarded the cell growth.
-
Cell Line:SMMC7721 cells
-
Concentration:3.3 μM
-
Incubation Time:24 hours
-
Result:Decreaed cyclin D3/E1,CDK2/4/6 protein expression and increased p21.
-
Cell Line:SMMC7721 and HepG2 cells
-
Concentration:3.3 μM; 10 μM
-
Incubation Time:24 hours
-
Result:Induced apoptosis in SMMC7721 and HepG2 cells.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Animal Model:BALB/c nude mice (weighing 18-20 g) subcutaneous injected by SMMC7721 cell suspension (5×106 cells/100 μL)[2]
-
Dosage:0.2 mg/kg
-
Administration:Subcutaneous injection; 20 days
-
Result:Suppressed tumor growth of SMMC7721 tumor xenografts.
Chemical Information
-
No. CAS 88979-61-7
-
Appearance Solid
-
Peso molecular 720.89
-
Fòrmula C39H60O12
-
Color White to off-white
-
SMILES
C[C@H]([C@@](C[C@H]([C@@H]1C)OC(/C=C/C(O)=O)=O)(O[C@@H]1C(C)C)O)[C@H](O)[C@@H]([C@](OC(/C(OC)=C/C(C)=C/[C@H]2C)=O)([H])[C@H](/C=C/C=C(C[C@@H]([C@@H]2O)C)\C)OC)C
-
Structure Classification
-
Initial Source
Streptomyces sp
-
Envío
Room temperature in continental US; may vary elsewhere.
-
Almacenamiento
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (1)
-
Journal Impact Factor
-
Most Recent
Solvente y solubilidad
In Vitro:
DMSO : 5 mg/mL (6.94 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
The following protocol is derived from the literature and is for reference only. It is recommended to first try a small sample.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
Pureza y Documentación
-
Ficha de datos (277 KB)
-
SDS (420 KB)
- English - EN (420 KB)
- Français - FR (420 KB)
- Deutsch - DE (420 KB)
- Norwegian - NO (420 KB)
- Español - ES (420 KB)
- Swedish - SV (420 KB)
- Italian - IT (420 KB)
- Korean - KR (420 KB)
- Portuguese - PT (420 KB)
-
Instrucciones de manejo (2659 KB)
Referencias
[1]. E J Bowman, et al. Bafilomycins: A Class of Inhibitors of Membrane ATPases From Microorganisms, Animal Cells, and Plant Cells. Proc Natl Acad Sci U S A. 1988 Nov;85(21):7972-6. [Content Brief]
[2]. Xiaoxiao Gao,et al.Bafilomycin C1 Induces G0/G1 Cell-Cycle Arrest and Mitochondrial-Mediated Apoptosis in Human Hepatocellular Cancer SMMC7721 Cells. J Antibiot (Tokyo). 2018 Sep;71(9):808-817. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.3872 mL | 6.9359 mL | 13.8717 mL | 34.6794 mL |
| 5 mM | 0.2774 mL | 1.3872 mL | 2.7743 mL | 6.9359 mL |
Bafilomycin C1 Related Classifications
HY-130173 Related Classifications
Powered by Bioz