Hydroxy-α-sanshool
Based on 3 publication(s) in Google Scholar
Hydroxy-α-sanshool is a transient receptor potential ankyrin 1 (TRPA1) and TRP vanilloid 1 (TRPV1) agonist with EC50s of 69 and 1.1 μM, respectively. Hydroxy-α-sanshool can be used for pain research.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza: 98.15%
- No. CAS: 83883-10-7
- Fòrmula: C16H25NO2
- Peso molecular:263.38
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Almacenamiento:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Hydroxy-α-sanshool
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Customer Validation & Images
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Bio/Physico-chemical Assay
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Bio/Physico-chemical Assay
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RT-PCR
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RT-PCR
Ver todos los productos específicos de isoformas Endogenous Metabolite
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Actividad biológica
Descripciòn
IC50 & Target
EC50: 69 µM (TRPA1), 1.1 µM (TRPV1)[1]
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| CHO | IC50 |
59 nM
Compound: 2
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Displacement of [3H]-CP55940 from human CB2 receptor expressed in CHO cells preincubated for 1 hr followed by radioligand addition measured after 1 hr by liquid scintillation counting analysis
Displacement of [3H]-CP55940 from human CB2 receptor expressed in CHO cells preincubated for 1 hr followed by radioligand addition measured after 1 hr by liquid scintillation counting analysis
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[PMID: 24175626] |
| CHO | IC50 |
7400 nM
Compound: 2
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Displacement of [3H]-Win55212-2 from human CB1 receptor expressed in CHO cells preincubated for 1 hr followed by radioligand addition measured after 1 hr by liquid scintillation counting analysis
Displacement of [3H]-Win55212-2 from human CB1 receptor expressed in CHO cells preincubated for 1 hr followed by radioligand addition measured after 1 hr by liquid scintillation counting analysis
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[PMID: 24175626] |
In Vitro
Hydroxy-α-sanshool (0-10 mM; 20 s) induces inward currents in cultured DRG neuron[2].
Hydroxy-α-sanshool (1 mM; 0-1 min) increases intracellular Ca2+ in cultured DRG neuron[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Dorsal root ganglion (DRG) neurons
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Concentration:0-10 mM
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Incubation Time:20 s
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Result:Induced small but robust inward currents for DRG neuron with an EC50 of 66.2 μM.
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Cell Line:Dorsal root ganglion (DRG) neurons
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Concentration:1 mM
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Incubation Time:0-1 min
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Result:Led to an intracellular Ca2+ increase in the majority of DRG neurons tested (98 of 100).
In Vivo
Hydroxy-α-sanshool (1 mM) is markedly aversive to WT mice, but in TRPV1 KO mice, the aversion is almost entirely eliminated (P<0.001)[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Wild-type and TRPV1-deficient mice[2]
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Dosage:4 mg per mouse
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Administration:Intradermal injection; 4 mg per mouse; once
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Result:Reduced paw-licking response in TRPV1-deficient mice (160 times, P < 0.05).
Showed paw-licking response in wild-type mice (mean 284.8 times) in 20 min.
Chemical Information
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No. CAS 83883-10-7
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Appearance Oil
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Peso molecular 263.38
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Fòrmula C16H25NO2
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Color White to yellow
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SMILES
C/C=C/C=C/C=C\CC/C=C/C(NCC(C)(O)C)=O
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Structure Classification
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Initial Source
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (3)
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Journal Impact Factor
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Most Recent
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Food Funct
Hydroxy-β-sanshool isolated from Zanthoxylum piperitum (Japanese pepper) shortens the period of the circadian clock. [Abstract]2022 Sep 22;13(18):9407-9418. PMID: 35960176
Hydroxy-α-sanshool purchased from MedChemExpress. Usage Cited in: Food Funct. 2022 Sep 22;13(18):9407-9418. [Abstract]
Hydroxy-β-sanshool (1, 3, 9 μg/mL; 24 h) upregulated Per2 promoter activity. NIH3T3 cells transfected with a Per2 promoter-reporter plasmid were stimulated with dexamethasone for 2 h, then incubated with hydroxyβ-sanshool for 24 h. Promoter activity was measured using bioluminescence.
Hydroxy-α-sanshool purchased from MedChemExpress. Usage Cited in: Food Funct. 2022 Sep 22;13(18):9407-9418. [Abstract]
Hydroxy-β-sanshool (1, 3, 9 μg/mL; 24 h) increased the mRNA expression of Bmal1, Per2, Cry1 and Per1 in NIH3T3 cells.
Hydroxy-α-sanshool purchased from MedChemExpress. Usage Cited in: Food Funct. 2022 Sep 22;13(18):9407-9418. [Abstract]
Hydroxy-β-sanshool (1, 3, 9 μg/mL; 24 h) increased the mRNA expression of Gpx1 and Sod2 in NIH3T3 cells.
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Foods
Stability of Hydroxy-α-Sanshool in Medium-Chain Triglyceride Oil and Corresponding Oil/Water Emulsions. [Abstract]2023 Sep 27;12(19):3589. PMID: 37835243 -
bioRxiv
2024 May 13:2023.08.17.553449. PMID: 37645855
Hydroxy-α-sanshool purchased from MedChemExpress. Usage Cited in: bioRxiv. 2024 May 13:2023.08.17.553449. [Abstract]
Hydroxy-α-sanshool (1300 μM) did not find obvious differences in the mean firing rates of lingual RA-LTMRs.
Solvente y solubilidad
In Vitro:
DMSO : 100 mg/mL (379.68 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (9.49 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (9.49 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (273 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Koo JY, et al. Compounds from Sichuan and Melegueta peppers activate, covalently and non-covalently, TRPA1 and TRPV1 channels. Br J Pharmacol. 2009 Aug;157(8):1398-409. [Content Brief]
[2]. Koo JY, et al. Hydroxy-alpha-sanshool activates TRPV1 and TRPA1 in sensory neurons. Eur J Neurosci. 2007 Sep;26(5):1139-47. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.7968 mL | 18.9840 mL | 37.9680 mL | 94.9199 mL |
| 5 mM | 0.7594 mL | 3.7968 mL | 7.5936 mL | 18.9840 mL | |
| 10 mM | 0.3797 mL | 1.8984 mL | 3.7968 mL | 9.4920 mL | |
| 15 mM | 0.2531 mL | 1.2656 mL | 2.5312 mL | 6.3280 mL | |
| 20 mM | 0.1898 mL | 0.9492 mL | 1.8984 mL | 4.7460 mL | |
| 25 mM | 0.1519 mL | 0.7594 mL | 1.5187 mL | 3.7968 mL | |
| 30 mM | 0.1266 mL | 0.6328 mL | 1.2656 mL | 3.1640 mL | |
| 40 mM | 0.0949 mL | 0.4746 mL | 0.9492 mL | 2.3730 mL | |
| 50 mM | 0.0759 mL | 0.3797 mL | 0.7594 mL | 1.8984 mL | |
| 60 mM | 0.0633 mL | 0.3164 mL | 0.6328 mL | 1.5820 mL | |
| 80 mM | 0.0475 mL | 0.2373 mL | 0.4746 mL | 1.1865 mL | |
| 100 mM | 0.0380 mL | 0.1898 mL | 0.3797 mL | 0.9492 mL |
Hydroxy-α-sanshool Related Classifications
HY-N6825 Related Classifications
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