Gypenoside L
Based on 4 publication(s) in Google Scholar
Gypenoside L is a saponin that can be found in Gynostemma pentaphyllum. Gypenoside L increases the SA-β-galactosidase activity, promotes the production of senescence-associated secretory cytokines. Gypenoside L also can activate p38 and ERK MAPK pathways and NF-κB pathway to induce senescence. Gypenoside L exhibits anti-tumor and anti-inflammatory activities.
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研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度 : 99.89%
- CAS 番号: 94987-09-4
- 分子式: C42H72O14
- 分子量:801.01
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保管条件:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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溶剤 & 溶解度
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- 純度とドキュメンテーション
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Help & FAQs
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MedChemExpress（MCE）の使用を引用している文献 Gypenoside L
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Cell Proliferation/Viability Assay
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Cell Imaging/Staining
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RT-PCR
生物活性
製品説明
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A549 | IC50 |
34.94 μg/mL
Compound: 3
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Cytotoxicity against human A549 cells after 48 hrs by MTT assay
Cytotoxicity against human A549 cells after 48 hrs by MTT assay
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[PMID: 24321343] |
| A549 | IC50 |
78.6 μg/mL
Compound: Gypenoside L
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Cytotoxicity against human A549 cells assessed as decrease in viable cells after 48 hrs by CCK-8 assay
Cytotoxicity against human A549 cells assessed as decrease in viable cells after 48 hrs by CCK-8 assay
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[PMID: 26922140] |
体外実験
Gypenoside L (20-80 μg/mL; 24 h) increases the mRNA expression levels of SASP, such as IL-1α, IL-6, TIMP-1, CXCL-1 and CXCL-2 in HepG2 and ECA-109 cells[1].
Gypenoside L (20-80 μg/mL; 24 h) causes cell cycle arrest at S phase[1].
GPL (3.125-100 μg/mL) significantly inhibits LPS-induced NO accumulation in RAW264.7 cells[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
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CAS 番号 94987-09-4
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性状 Solid
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分子量 801.01
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分子式 C42H72O14
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Color White to off-white
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SMILES
C[C@]12[C@]3([C@@]([C@@H](C[C@]1([H])[C@@]4([C@@](C(C)([C@H]([C@@H](C4)O)O[C@H]5[C@@H]([C@H]([C@@H]([C@H](O5)CO)O)O)O[C@@H]6O[C@@H]([C@H]([C@@H]([C@H]6O)O)O)CO)C)([H])CC2)C)O)([H])[C@@](CC3)([H])[C@@](C)(O)CC/C=C(C)\C)C
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Structure Classification
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Initial Source
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (4)
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Journal Impact Factor
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Most Recent
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Antioxid Redox Signal
TXNIP exacerbates the senescence and ageing-related dysfunction of β-cells by inducing cell cycle arrest through p38-p16/p21-CDK-Rb pathway. [Abstract]2023 Mar;38(7-9):480-495. PMID: 36070438 -
Int J Mol Sci
Inhibitory Effects of Heat-Processed Gynostemma pentaphyllum Extract (Actiponin®) and Its Components on Cartilage Breakdown in Osteoarthritis. [Abstract]2025 Feb 18;26(4):1728. PMID: 40004191 -
Bioorg Chem
Gypenoside L inhibits oral squamous cell carcinoma progression by targeting the AKT signaling pathway. [Abstract]2026 Jan:168:109303. PMID: 41343873
Gypenoside L purchased from MedChemExpress. Usage Cited in: Bioorg Chem. 2026 Jan:168:109303. [Abstract]
Cell viability of HN6, CAL27, HOK, and HGF cells detected by CCK-8 after 48 and 72 h treated with different concentration Gypenoside L (0.01-1000 μM).
Gypenoside L purchased from MedChemExpress. Usage Cited in: Bioorg Chem. 2026 Jan:168:109303. [Abstract]
Colony formation for 14 days after plating cell incubated with Gypenoside L (0, 20, 40 μM).
Gypenoside L purchased from MedChemExpress. Usage Cited in: Bioorg Chem. 2026 Jan:168:109303. [Abstract]
Flow cytometry detected apoptosis after Gypenoside L (0, 20, 40 μM). Incubation.
Gypenoside L purchased from MedChemExpress. Usage Cited in: Bioorg Chem. 2026 Jan:168:109303. [Abstract]
Cleaved-casepase-3 and total casepase-3 protein expression was examined by Western-bloting after treated with Gypenoside L (0, 20, 40 μM).
Gypenoside L purchased from MedChemExpress. Usage Cited in: Bioorg Chem. 2026 Jan:168:109303. [Abstract]
RNA expression level of CCNE1 was detected by RT-PCR treated with Gypenoside L (0, 20, 40 μM).
Gypenoside L purchased from MedChemExpress. Usage Cited in: Bioorg Chem. 2026 Jan:168:109303. [Abstract]
Macroscopic of tumor size and bargraph of tumor volume during the experiment treated with Gypenoside L (10, 20 mg/kg, i.p.).
Gypenoside L purchased from MedChemExpress. Usage Cited in: Bioorg Chem. 2026 Jan:168:109303. [Abstract]
Ki-67 staining, H/E staining were examined in paraffin-embedded tumor tissues treated with Gypenoside L (10, 20 mg/kg, i.p.).
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Cytokine
Exosomes from adipose-derived mesenchymal stem cells improve liver fibrosis by regulating the miR-20a-5p/TGFBR2 axis to affect the p38 MAPK/NF-κB pathway. [Abstract]2023 Dec:172:156386. PMID: 37852157
溶剤 & 溶解度
体外:
DMSO : 100 mg/mL (124.84 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
体内:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (3.12 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (3.12 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション
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取扱説明書 (2659 KB)
参考文献
[1]. Ma J, et, al. Gypenoside L Inhibits Proliferation of Liver and Esophageal Cancer Cells by Inducing Senescence. Molecules. 2019 Mar 18;24(6):1054. [Content Brief]
[2]. Shen CY, et, al. Comparison of the Effects and Inhibitory Pathways of the Constituents from Gynostemma pentaphyllum against LPS-Induced Inflammatory Response. J Agric Food Chem. 2018 Oct 31;66(43):11337-11346. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.2484 mL | 6.2421 mL | 12.4842 mL | 31.2106 mL |
| 5 mM | 0.2497 mL | 1.2484 mL | 2.4968 mL | 6.2421 mL | |
| 10 mM | 0.1248 mL | 0.6242 mL | 1.2484 mL | 3.1211 mL | |
| 15 mM | 0.0832 mL | 0.4161 mL | 0.8323 mL | 2.0807 mL | |
| 20 mM | 0.0624 mL | 0.3121 mL | 0.6242 mL | 1.5605 mL | |
| 25 mM | 0.0499 mL | 0.2497 mL | 0.4994 mL | 1.2484 mL | |
| 30 mM | 0.0416 mL | 0.2081 mL | 0.4161 mL | 1.0404 mL | |
| 40 mM | 0.0312 mL | 0.1561 mL | 0.3121 mL | 0.7803 mL | |
| 50 mM | 0.0250 mL | 0.1248 mL | 0.2497 mL | 0.6242 mL | |
| 60 mM | 0.0208 mL | 0.1040 mL | 0.2081 mL | 0.5202 mL | |
| 80 mM | 0.0156 mL | 0.0780 mL | 0.1561 mL | 0.3901 mL | |
| 100 mM | 0.0125 mL | 0.0624 mL | 0.1248 mL | 0.3121 mL |
Gypenoside L 関連分類
HY-N8211 関連分類
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