Isosalvianolic acid C
Isosalvianolic acid C is a naturally derived phenolic compound that acts as a signal modulator, antioxidant, and enzyme inhibitor. Isosalvianolic acid C induces the phosphorylation of PLC-γ, IP3R, PKC, and P38, triggering Ca2+ mobilization, degranulation, and chemokine release. Isosalvianolic acid C activates MRGPRX2 and its murine homolog MrgprB2, leading to pseudo-allergic reactions in vivo. Isosalvianolic acid C inhibits Cu2+-induced LDL peroxidation. Isosalvianolic acid C can be used in studies related to pseudo-allergy, diabetes, and atherosclerosis.
For research use only. We do not sell to patients.
- CAS No.: 142115-17-1
- Formula: C26H20O10
- Molecular Weight:492.43
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Biological Activity
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p38 |
PKC |
Isosalvianolic acid C (50-200 μM; 30 min) induces degranulation of the human mast cell line LAD2 in a dose-dependent manner, and stimulates histamine release from the human mast cell line LAD2 in a dose-dependent manner[1].
Isosalvianolic acid C (50-200 μM; 12 h) dose-dependently induces MCP-1 release from human mast cell line LAD2[1].
Isosalvianolic acid C (50-200 μM; acute bath application) induces dose-dependent intracellular Ca2+ mobilization in HEK293-MRGPRX2 and HEK293-MrgprB2 cells[1].
Isosalvianolic acid C binds to the MRGPRX2 receptor with a KD value of 12.77×10-5 M[1].
Isosalvianolic acid C (50-200 μM; 16 h) dose-dependently activates the PLCγ, IP3R, PKC and P38 signaling pathways in LAD2 human mast cells[1].
Isosalvianolic acid C (50-200 μM; 30 min) induces degranulation, MCP-1 release and histamine release in human LAD2 mast cells in an MRGPRX2-dependent manner[1].
Isosalvianolic acid C methyl ester, namely (8'R)-Isosalvianolic acid C methyl ester, exerts potent inhibitory activity against purified α-glucosidase with an IC50 of 0.11 μM, and also blocks AGE formation in the bovine serum albumin-glucose reaction system with an IC50 of 0.07 μM[2].
Isosalvianolic acid C (compound 6) (1 h preincubation; 2 h Cu2+ incubation) inhibits Cu2+-induced peroxidation of human low-density lipoprotein (LDL), with an IC50 value of 2.72 μM[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:LAD2 human mast cells
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Concentration:50, 100, 200 μM
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Incubation Time:16 h
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Result:Significantly increased the levels of phosphorylated PLCγ1, phosphorylated IP3R, phosphorylated PKC, and phosphorylated P38 in a dose-dependent manner, with all phosphorylated protein levels significantly higher than control levels.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:C57BL/6 (WT, adult, 25-30 g)
C57BL/6-MrgprB2 knockout (MUT, adult, 25-30 g)
C57BL/6-KitW-sh/W-sh (adult, 25-30 g)[1] -
Dosage:0.21, 0.52, 1.04 mg/kg (systemic anaphylaxis)
0.5, 1, 2.0 mg/mL (hindpaw inflammation) -
Administration:i.v. via tail vein; single dose (systemic anaphylaxis)
intraplantar; single dose (hindpaw inflammation) -
Result:Increased Evans blue leakage into ears in a dose-dependent manner in systemic anaphylaxis model treated with 0.52 mg/kg reagent.
Triggered dose-dependent hindpaw swelling with paw thickness increase rates of 15%, 16%, and 18% respectively at 0.5 mg/mL, 1.0 mg/mL, and 2.0 mg/mL doses.
Induced dose-dependent Evans blue extravasation with OD620 nm per g paw weight values of 2.5, 2.5, and 3.5 respectively at 0.5 mg/mL, 1.0 mg/mL, and 2.0 mg/mL doses.
Almost completely eliminated hindpaw inflammation in mast cell-deficient C57BL/6-KitW-sh/W-sh mice.
Resulted in almost no hindpaw inflammation in MrgprB2 knockout mice treated with 0.5 mg/mL reagent.
Chemical Information
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CAS No. 142115-17-1
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Molecular Weight 492.43
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Formula C26H20O10
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SMILES
OC(C=C1C[C@H](C(O)=O)OC(/C=C/C2=C3C(OC4=CC(O)=C(O)C=C4C=C3)=C(C=C2)O)=O)=C(C=C1)O
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
[1]. Lin Y, et al. Isosalvianolic acid C-induced pseudo-allergic reactions via the mast cell specific receptor MRGPRX2. International immunopharmacology. 2019 Jun;71:22-31. [Content Brief]
[2]. Ma HY, et al. Constituents with α-glucosidase and advanced glycation end-product formation inhibitory activities from Salvia miltiorrhiza Bge. Journal of natural medicines. 2011 Jan;65(1):37-42. [Content Brief]
[3]. Lin YL, et al. Anti-lipid-peroxidative principles from Tournefortia sarmentosa. Journal of natural products. 2002 May;65(5):745-7. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Isosalvianolic acid C Related Classifications
HY-122967 Related Classifications
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- Isosalvianolic acid C
- 142115-17-1
- PKC
- Mas-related G-protein-coupled Receptor (MRGPR)
- p38 MAPK
- Phospholipase
- LDL peroxidation
- HEK293-MRGPRX2 cells
- LAD2 human mast cells
- pseudo-allergic reactions
- α-glucosidase
- MRGPRX2
- HEK293-MrgprB2 cells
- MrgprB2
- advanced glycation end-product
- diabetes mellitus
- Inhibitor
- inhibitor
- inhibit