Anticancer agent-367
Anticancer agent-367 is a 3-hydroxy-3′,4′,5′-trimethoxyflavone derivative with selective cytotoxicity against osteosarcoma cells (IC50 = 14 μM). Anticancer agent-367 reduces AKT phosphorylation levels in 143B osteosarcoma cells and downregulates the expression of epithelial-mesenchymal transition-related genes such as ZEB1, MMP2, and MMP9. The combination of Anticancer agent-367 with Doxorubicin (HY-15142A) produces a synergistic antiproliferative effect on 143B cells. Anticancer agent-367 can be used in osteosarcoma-related research.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 分子式: C23H25NO6
- 分子量:411.45
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保管条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
製品説明
IC50 & Target
[1]|
Akt |
MMP-9 |
MMP-2 |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
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| 143B | IC50 |
14 μM
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Cytotoxicity against human 143B osteosarcoma cells assessed as viability reduction after 48 hrs by WST-8/CCK-8 assay.
Cytotoxicity against human 143B osteosarcoma cells assessed as viability reduction after 48 hrs by WST-8/CCK-8 assay.
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42664600 |
| 143B | IC50 |
2.0 μM
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Antiproliferative activity against human 143B osteosarcoma cells assessed as growth inhibition after 48 hrs by SRB assay.
Antiproliferative activity against human 143B osteosarcoma cells assessed as growth inhibition after 48 hrs by SRB assay.
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42664600 |
体外実験
Anticancer agent-367 (compound 6A) (0-160 μmol/L) inhibits 143B osteosarcoma cell viability and growth, with a WST-8 IC50 of 14 μmol/L and an SRB IC50 of 2.0 μmol/L; it exhibits low toxicity toward MRC-5 lung fibroblasts[1].
Anticancer agent-367 (0-56 μmol/L) produced a synergistic effect with doxorubicin in 143B osteosarcoma cells, with CI = 0.9272 and DRI >1, supporting a reduction in the doxorubicin dose[1].
Anticancer agent-367 (7-14 μmol/L; 48 h) reduces p-AKT in 143B osteosarcoma cells at 14 μmol/L alone or in combination with doxorubicin[1].
Anticancer agent-367 (7-14 μmol/L; 48 h) downregulates the EMT-related genes ZEB1, MMP2, and MMP9 in 143B osteosarcoma cells, and further downregulates MMP9[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:143B human osteosarcoma cells, MRC-5 human fetal lung fibroblasts
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Concentration:0, 10, 20, 40, 80, 160 μmol/L
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Incubation Time:48 h
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Result:Produced dose-dependent inhibition of 143B cell viability and growth.
Showed low toxicity toward MRC-5 cells.
At 80 μmol/L, resulted in 5% inhibition of cell viability.
Displayed lower cytotoxicity than 6b at higher concentrations (>80 μmol/L).
Below 40 μmol/L, did not reduce MRC-5 cell viability.
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Cell Line:143B human osteosarcoma cells
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Concentration:7 and 14 μmol/L
0.075 and 0.15 μmol/L Doxorubicin -
Incubation Time:48 h
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Result:Reduced p‑AKT levels showed no significant change when compound 6a was applied as a single agent at 7 μmol/L.
Reduced p‑AKT levels significantly when 7 μmol/L compound 6a was combined with Doxorubicin.
Reduced p‑AKT expression markedly at 14 μmol/L regardless of monotherapy or Doxorubicin combination, which indicated the inhibition of AKT signaling.
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Cell Line:143B human osteosarcoma cells
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Concentration:7 and 14 μmol/L
0.075 and 0.15 μmol/L Doxorubicin -
Incubation Time:48 h
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Result:Downregulated ZEB1, MMP2, and MMP9 in a concentration‑dependent manner when used as a single agent.
Exhibited similar down‑regulation trends for MMP2 and MMP9 expression under combination with doxorubicin, yet produced no significant influence on ZEB1 levels.
Exerted additional suppressive effect on MMP9 expression.
化学情報
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分子量 411.45
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分子式 C23H25NO6
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SMILES
O=C1C2=CC=C(N3CCCCC3)C=C2OC(C4=CC(OC)=C(OC)C(OC)=C4)=C1O
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Please store the product under the recommended conditions in the Certificate of Analysis.
純度とドキュメンテーション
参考文献
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
Anticancer agent-367 関連分類
HY-189737 関連分類
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