PF1070A

PF1070A (compound 6) potently inhibits the growth of the multidrug-resistant Plasmodium falciparum Dd2 strain and the drug-sensitive Plasmodium falciparum 3D7 strain after 48-72 h of treatment, with EC₅₀ values of 0.091 μM and 0.069 μM, respectively. Furthermore, PF1070A exhibits much higher selectivity for the parasite than for human HepG2 cells, with a selectivity index of 150^[1].
PF1070A (24-48 h) exerts a reversible anti-proliferative inhibitory effect on asynchronous Plasmodium falciparum strain Dd2; parasite parasitemia rebounds following the removal of PF1070A after 24 h or 48 h of treatment^[1].

PF1070A (added at 6, 18, 30, or 42 HPI) inhibits the growth of Plasmodium falciparum Dd2 across all intraerythrocytic stages prior to late schizogony, with the strongest inhibitory effect when administered before the trophozoite stage; administration at 6 HPI results in cell cycle arrest prior to nuclear division^[1].

PF1070A (2 h) potently inhibits the catalytic activity of recombinant PfHDAC1, with an IC₅₀ of 0.0011 μM^[1].
PF1070A (4 h) induces hyperacetylation of histone H4 in synchronized cultures of Plasmodium falciparum 3D7 at the trophozoite stage^[1].
PF1070A induces perinuclear histone H4 hyperacetylation and rearrangement of the microtubule cytoskeleton in synchronized cultures of Plasmodium falciparum 3D7, which is consistent with chromatin remodeling mediated by HDAC inhibition^[1].
After incubation with mouse liver microsomes for 1 h, the remaining percentage of PF1070A (0.1 mM; 1 h for the microsomal stability assay; 200 μM; 24 h for the kinetic solubility assay) is 30.3%, and its kinetic solubility in PBS (pH 7.4) at room temperature is 51.8 μM^[1].
PF1070A (50-500 µg; 4 days) inhibits root growth of Tachinagaha soybean seedlings in a concentration-dependent manner^[1].
PF1070A (10-80 µg; 1 week) induces chlorosis in Tachinagaha soybean leaves in a concentration-dependent manner over a 1-week incubation period^[1].
PF1070A (50-500 µg; 4 days) inhibits root growth of Fukuyutaka, Enrei, and Harosoy soybean seedlings, with similar IC₅₀ values (721, 741, and 754 µg/mL, respectively) over the 4-day incubation period, despite differences in the susceptibility of these soybean cultivars to Calonectria ilicicola^[1].
PF1070A (administered for 1 month) is produced by the Calonectria ilicicola strain, and its yield ranges from 0.01 to 4.46 mg/100 mL after 1 month of cultivation in PDB at 25°C in the dark^[1].
When PF1070A (9 μM; 8-24 h) is combined with zinc ions, cadmium ions, or Dexamethasone (HY-14648), it synergistically enhances the activity of the MT-I promoter and the level of MT-I mRNA in mouse L13-17 cells^[2].
PF1070A (30 μM; 24 h) activates the mouse MT-I promoter in mouse Ltk⁻ cells via two specific regions (positions -150/-149 and -49/-43), and this mechanism differs from the MREs-mediated zinc activation mechanism^[2].
PF1070A (3-9 μM; 24 h) enhances the activity of the MT-I promoter in mouse L13-17 cells^[2].
PF1070A (90 μM) induces the production of MT mRNA and MT protein in human HeLa-S3 cells, an effect similar to that observed in mouse L13-17 cells^[2].
PF1070A (90 μM, 9 μM + 70 μM ZnCl₂; 6 h) does not modulate the intracellular zinc concentration in mouse L13-17 cells, indicating that its metallothionein (MT) induction activity is independent of zinc mobilization^[2].
PF1070A (5 nM; combined with 10 nM CdCl₂) induces exogenous BCL-6 expression, thereby suppressing endogenous BCL-6 levels in BT549 breast cancer cells^[3].