CMIT/MIT (14.5% in water)
Based on 1 Customer Validation
CMIT/MIT is a CMIT and MIT mixture. CMIT and MIT are powerful synthetic biocidal substances. CMIT and MIT are used as preservatives in various cosmetics and industrial products. CMIT and MIT are present in many water-soluble consumer products, such as shampoos, toothpastes. CMIT/MIT (3:1 mixture of CMIT and MIT) produces mitochondrial ROS via inhibiting mitochondrial complex I and II. CMIT/MIT (in 3:1 ratio) induces neurotoxicity through the upregulation of the MAPKs signaling pathways. CMIT/MIT can be used in the research of respiratory diseases and neuroblastoma.
For research use only. We do not sell to patients.
- Purity: 14.36%
- CAS No.: 55965-84-9
- Formula: C8H9ClN2O2S2
- Molecular Weight:264.75
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Storage:Pure form -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Anti-Infection Compound Library
HY-L002
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Immunology/Inflammation Compound Library
HY-L007
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MAPK Compound Library
HY-L010
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Metabolism/Protease Compound Library
HY-L012
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NF-κB Signaling Compound Library
HY-L014
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Stem Cell Signaling Compound Library
HY-L017
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Anti-Aging Compound Library
HY-L034
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Reprogramming Compound Library
HY-L039
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Oxygen Sensing Compound Library
HY-L045
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Antibacterial Compound Library
HY-L049
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Pyroptosis Compound Library
HY-L059
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Glutamine Metabolism Compound Library
HY-L064
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Anti-Lung Cancer Compound Library
HY-L075
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Anti-Cancer Metabolism Compound Library
HY-L083
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Anti-Parkinson's Disease Compound Library
HY-L085
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Neurodegenerative Disease-related Compound Library
HY-L086
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Angiogenesis-Related Compound Library
HY-L088
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Mitochondria-Targeted Compound Library
HY-L089
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Glucose Metabolism Compound Library
HY-L092
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Inactive Ingredient Library
HY-L096
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Osteogenesis Compound Library
HY-L131
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Cuproptosis Compound Library
HY-L133
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Cancer Stem Cells Compound Library
HY-L135
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Pain-Related Compound Library
HY-L139
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Mitochondrial Protection Compound Library
HY-L144
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Metabolic Enzyme Compound Library
HY-L146
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Bioactive Compound Library Max
HY-L181
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MCE Bioactive Compound Library
HY-L001V
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Bioactive Compound Library
HY-L001
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Protein Kinase Compound Library
HY-L196
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Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library
HY-L199
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Cell Proliferation Compound Library
HY-L201
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High-Throughput Bioactive Compound Library
HY-L205
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Environmental Pollutant Compound Library
HY-L247
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Lactylation Compound Library
HY-L249
Biological Activity
CMIT/MIT is a CMIT and MIT mixture. CMIT and MIT are powerful synthetic biocidal substances. CMIT and MIT are used as preservatives in various cosmetics and industrial products. CMIT and MIT are present in many water-soluble consumer products, such as shampoos, toothpastes. CMIT/MIT (3:1 mixture of CMIT and MIT) produces mitochondrial ROS via inhibiting mitochondrial complex I and II. CMIT/MIT (in 3:1 ratio) induces neurotoxicity through the upregulation of the MAPKs signaling pathways. CMIT/MIT can be used in the research of respiratory diseases and neuroblastoma[1][2][3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 55965-84-9
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Appearance Liquid (Density: 1.19 g/cm3)
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Molecular Weight 264.75
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Formula C8H9ClN2O2S2
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Color Colorless to light yellow
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SMILES
O=C1C=CSN1C.O=C2C=C(SN2C)Cl
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Synonyms
CMI/MI
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Pure form -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 200 mg/mL (755.43 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (18.89 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (18.89 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (274 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Portuguese - PT (396 KB)
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Handling Instructions (2659 KB)
References
[1]. Kim D, et al. CMIT/MIT produces mitochondrial ROS via inhibiting mitochondrial complex I and II. Free Radic Biol Med. 2025 Dec 23;244:505-515. [Content Brief]
[3]. Molinari F, et al. Biocide mixture (CMIT/MIT) induces neurotoxicity through the upregulation of the MAPKs signaling pathways. J Neurophysiol. 2025 Jul 1;134(1):183-192. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.7771 mL | 18.8857 mL | 37.7715 mL | 94.4287 mL |
| 5 mM | 0.7554 mL | 3.7771 mL | 7.5543 mL | 18.8857 mL | |
| 10 mM | 0.3777 mL | 1.8886 mL | 3.7771 mL | 9.4429 mL | |
| 15 mM | 0.2518 mL | 1.2590 mL | 2.5181 mL | 6.2952 mL | |
| 20 mM | 0.1889 mL | 0.9443 mL | 1.8886 mL | 4.7214 mL | |
| 25 mM | 0.1511 mL | 0.7554 mL | 1.5109 mL | 3.7771 mL | |
| 30 mM | 0.1259 mL | 0.6295 mL | 1.2590 mL | 3.1476 mL | |
| 40 mM | 0.0944 mL | 0.4721 mL | 0.9443 mL | 2.3607 mL | |
| 50 mM | 0.0755 mL | 0.3777 mL | 0.7554 mL | 1.8886 mL | |
| 60 mM | 0.0630 mL | 0.3148 mL | 0.6295 mL | 1.5738 mL | |
| 80 mM | 0.0472 mL | 0.2361 mL | 0.4721 mL | 1.1804 mL | |
| 100 mM | 0.0378 mL | 0.1889 mL | 0.3777 mL | 0.9443 mL |
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