Cat. No.: HY-W017982

CMIT/MIT (14.5% in water)

(Synonyms: CMI/MI)

Customer Review

Based on 1 Customer Validation

CMIT/MIT (14.5% in water) (CMI/MI) is a mixture of CMIT and MIT, and is also a preservative and skin sensitizer. CMIT/MIT (14.5% in water) increases the levels of phosphorylated ERK1/2, p38, JNK1/2, p53, p21 and Bax, decreases the level of Bcl-2, and activates the Nrf-2/HO-1 signaling pathway. CMIT/MIT (14.5% in water) increases LDH release and lipid peroxidation levels, impairs antioxidant defense capacity, promotes pro-inflammatory cytokine release, induces apoptosis, triggers cell cycle arrest, exhibits neurotoxicity in neuroblastoma cells, and causes dysregulation of Th2/Th17 immune responses. CMIT/MIT (14.5% in water) can be used in studies related to allergy, atopic dermatitis and lung injury.

For research use only. We do not sell to patients.

Purity: 14.36%
CAS No.: 55965-84-9
Formula: C₈H₉ClN₂O₂S₂
Molecular Weight:264.75

Storage:

Pure form -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

CMIT/MIT (14.5% in water) Chemical Structure

CAS No.: 55965-84-9

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

ERK1

ERK2

JNK1

JNK2

Bax

Bcl-2

IL-4

IL-17A

IL-6

IL-1β

All ERK Isoforms :

All JNK Isoforms :

All Bcl-2 Family Isoforms :

All Interleukin Related Isoforms :

In Vitro

CMIT/MIT (14.5% in water) can increase the levels of phosphorylated ERK1/2, p38, JNK1/2, p53, p21 and Bax, decrease the level of Bcl-2, and activate the Nrf-2/HO-1 signaling pathway^[1].
CMIT/MIT (14.5% in water) increases the mutation frequency of mouse lymphoma cells by up to 10-fold^[3].
CMIT/MIT (14.5% in water) does not induce chromosomal aberrations in Chinese hamster lung fibroblasts, although it causes cytotoxicity at concentrations ranging from 0.015 to 0.12 a.i. μg/mL^[3].
CMIT/MIT (14.5% in water) exhibits high skin permeability in rat skin^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

CMIT/MIT (0.1875 mg/kg/day; transdermal administration; 5 days per week for 3 weeks) (14.5% in water) induces atopic dermatitis (AD)-like skin inflammation and Th2-related immune dysfunction in normal BALB/c mice; it promotes Th2/Th17-associated immune dysregulation, including a significant increase in serum total IgE levels and upregulated expression of IL-4/IL-17A in the skin^[2].
CMIT/MIT (1.5-15 a.i. mg/kg bw/day; oral administration; daily dosing from gestational day 5 to 15) (14.5% in water) causes dose-dependent maternal death in pregnant rats, but shows no teratogenicity or reproductive toxicity^[3].
CMIT/MIT (1.5-13.3 a.i. mg/kg bw/day; administered orally; daily dosing on gestational days 6-18) (14.5% in water) causes severe dose-dependent maternal mortality and fetal loss in pregnant rabbits^[3].
CMIT/MIT (14.5% in water) administered orally to dogs at doses ≥3.5 a.i. mg/kg bw/day over a two-week period triggers mild systemic adverse responses such as lowered food intake as well as changes in hematological and serum biochemical indicators^[3].
CMIT/MIT (14.5% in water) causes dose-dependent gastric irritation in rats at doses of 100 and 300 a.i. ppm, but shows no carcinogenicity or systemic toxicity following 2 years of oral administration^[3].
CMIT/MIT (14.5% in water) is a potent skin sensitizer in mice, with an EC₃ range of 0.75-2.1 a.i. μg/cm² in the local lymph node assay; dermal administration at a dose of 400 a.i. ppm for 30 months causes skin hyperplasia and inflammation in mice but no carcinogenicity; dermal administration at a dose of ≥ 0.56 a.i. mg/kg bw/day for 3 weeks induces moderate skin irritation in rabbits without associated systemic toxicity^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

BALB/c (female, 5 weeks old, 16-20 g)^[2]
Dosage:

0.1875 mg/kg/day
Administration:

epicutaneous; 5 days/week; 3 weeks
Result:

Induced AD-like skin lesions with significantly increased transepidermal water loss (TEWL), total serum IgE levels, mast cell counts, and mast cell degranulation.
Significantly elevated skin mRNA expression of Th2-related cytokines/chemokines: thymic stromal lymphopoietin (TSLP), IL-6, IL-13, and CCL17.
Increased CD4⁺IL-4⁺ cell population in skin-draining lymph nodes.

Animal Model:

BALB/c (female, 5 weeks old, 16-20 g, induced via 3 intraperitoneal sensitizations with ovalbumin (OVA) plus aluminum hydroxide at 1-week intervals, followed by 2 epicutaneous OVA sensitizations at 1-week intervals)^[2]
Dosage:

0.1875 mg/kg/day
Administration:

epicutaneous; 5 days/week; 3 weeks
Result:

Aggravated AD-like skin lesions with significantly higher TEWL, clinical scores, inflammatory cell infiltration, mast cell counts, and total serum IgE levels compared to mice treated with OVA alone.
Significantly increased skin mRNA expression of IL-4 and IL-17A compared to mice treated with OVA alone.
Increased CD4⁺IL-4⁺ and CD4⁺IL-17A⁺ cell populations in skin-draining lymph nodes compared to mice treated with OVA alone.

Chemical Information

CAS No. 55965-84-9
Appearance Liquid (Density: 1.19 g/cm³)
Molecular Weight 264.75
Formula C₈H₉ClN₂O₂S₂
Color Colorless to light yellow
SMILES

O=C1C=CSN1C.O=C2C=C(SN2C)Cl
Synonyms

CMI/MI
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Pure form	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 200 mg/mL (755.43 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.7771 mL

18.8857 mL

37.7715 mL

5 mM

0.7554 mL

3.7771 mL

7.5543 mL

10 mM

0.3777 mL

1.8886 mL

3.7771 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 5 mg/mL (18.89 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 5 mg/mL (18.89 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 5 mg/mL (18.89 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (280 KB)
SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Molinari F, et al. Biocide mixture (CMIT/MIT) induces neurotoxicity through the upregulation of the MAPKs signaling pathways. Journal of neurophysiology. 2025 Jul 01;134(1):183-192. [Content Brief]

[2]. Go HN, et al. Effects of chloromethylisothiazolinone/methylisothiazolinone (CMIT/MIT) on Th2/Th17-related immune modulation in an atopic dermatitis mouse model. Scientific reports. 2020 Mar 05;10(1):4099. [Content Brief]

[3]. Kim M K, et al. Risk Assessment of 5-Chloro-2-Methylisothiazol-3(2H)-One/2-Methylisothiazol-3(2H)-One (CMIT/MIT) Used as a Preservative in Cosmetics. Toxicological Research, 2019, 35(2): 103-117.

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.7771 mL	18.8857 mL	37.7715 mL	94.4287 mL
	5 mM	0.7554 mL	3.7771 mL	7.5543 mL	18.8857 mL
	10 mM	0.3777 mL	1.8886 mL	3.7771 mL	9.4429 mL
	15 mM	0.2518 mL	1.2590 mL	2.5181 mL	6.2952 mL
	20 mM	0.1889 mL	0.9443 mL	1.8886 mL	4.7214 mL
	25 mM	0.1511 mL	0.7554 mL	1.5109 mL	3.7771 mL
	30 mM	0.1259 mL	0.6295 mL	1.2590 mL	3.1476 mL
	40 mM	0.0944 mL	0.4721 mL	0.9443 mL	2.3607 mL
	50 mM	0.0755 mL	0.3777 mL	0.7554 mL	1.8886 mL
	60 mM	0.0630 mL	0.3148 mL	0.6295 mL	1.5738 mL
	80 mM	0.0472 mL	0.2361 mL	0.4721 mL	1.1804 mL
	100 mM	0.0378 mL	0.1889 mL	0.3777 mL	0.9443 mL

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CMIT/MIT (14.5% in water)

Bioactive Compound Library Max

Bioactive Compound Library

Apoptosis Compound Library

Immunology/Inflammation Compound Library

MAPK Compound Library

NF-κB Signaling Compound Library

Stem Cell Signaling Compound Library

Anti-Aging Compound Library

Antioxidant Compound Library

Oxygen Sensing Compound Library

Ferroptosis Compound Library

Glutamine Metabolism Compound Library

Neuroprotective Compound Library

Anti-Pancreatic Cancer Compound Library

Anti-Cancer Metabolism Compound Library

Anti-Obesity Compound Library

Angiogenesis-Related Compound Library

Mitochondria-Targeted Compound Library

Transcription Factor-Targeted Library

Inactive Ingredient Library

Antidepressant Compound Library

Anti-Prostate Cancer Compound Library

Anti-Pulmonary Fibrosis Compound Library

Osteogenesis Compound Library

Pain-Related Compound Library

Cell Death Library

Multi-Target Compound Library

Radioprotector Library

Mitophagy Compound Library

MCE Bioactive Compound Library

Anti-Fibrosis Compound Library

Protein Kinase Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

Cell Proliferation Compound Library

High-Throughput Bioactive Compound Library

Anti-Rheumatic Arthritis Compound Library

Lactylation Compound Library

All ERK Isoforms

All JNK Isoforms

All Bcl-2 Family Isoforms

All Interleukin Related Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

CMIT/MIT (14.5% in water) Related Classifications

HY-W017982 Related Classifications