Tubulysin A
Based on 1 publication(s) in Google Scholar
Tubulysin A (TubA) is an anticancer and antiangiogenic agent with anti-microtubule, anti-mitosis and anti-proliferative activity against a variety of cancer cells with IC50 values in the pmol range. It can induce apoptosis of cancer cells and has no effect on normal cells. Tubulysins are a group of potent cytotoxins consisting of nine members (A-I). Tubulysin A can synthesize ADC as ADC Cytotoxin.
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- Purity: 99.56%
- CAS No.: 205304-86-5
- 화학식: C43H65N5O10S
- 분자량:844.07
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보관:
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
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Biological Activity
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Chemical Information
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용액&용해도
- 순도&문서
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Tubulysin A
MoreAll Antibiotic Isoforms
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Biological Activity
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Traditional Cytotoxic Agents |
The IC50 values of Tubulysin A in the NCI-H1299 (lung), HT-29 (colon) and A2780 (ovary) cell lines are 3, 1 and 2 nmol/L, respectively[4].
The IC50 values of Tubulysin A against L929 (mouse fibroblast) and KB-V1 (human cervical cancer multidrug resistant cell line) cells were 0.07 and 1.4 ng/ml, respectively[1].
Tubulysin A (1 nM, 10 nM; 24h) has an antiangiogenic effect in HUVEC cells with IC50 values of 2.07-2.97 nM[1].
Tubulysin A (5h) can inhibit cell migration in HUVEC cells with IC50 value of 2.26 nM[1].
Tubulysin A (72h) can inhibit cell growth in HUVEC cells with GI50 value of 0.34 nM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HL-60 cells
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Concentration:0-100 nM
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Incubation Time:24/48h
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Result:Had a strong pro-apoptotic effect on HL-60 tumor cell line, but had no significant effect on HUVEC cell line.
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Cell Line:
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Concentration:
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Incubation Time:
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Result:
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Mouse xenotransplantation model[1]
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Dosage:0.04 and 0.06 mg/kg
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Administration:Intraperitoneal (i.p.)
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Result:Inhibited the growth of tumor cells.
Chemical Information
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CAS No. 205304-86-5
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Appearance Solid
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분자량 844.07
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화학식 C43H65N5O10S
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Color White to light yellow
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SMILES
O=C([C@@]([C@@H](C)CC)([H])NC([C@@H](CCCC1)N1C)=O)N(COC(CC(C)C)=O)[C@@H](C(C)C)C[C@H](C2=NC(C(N[C@H](C[C@H](C)C(O)=O)CC3=CC=C(O)C=C3)=O)=CS2)OC(C)=O
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Synonyms
TubA
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선적
Room temperature in continental US; may vary elsewhere.
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보관
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Publications (1)
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Journal Impact Factor
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Most Recent
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Folia Histochem Cytobiol
HDAC6 inhibition alleviates acute pulmonary embolism: a possible future therapeutic option. [Abstract]2023;61(1):56-67. PMID: 36880683
용액&용해도
DMSO : 100 mg/mL (118.47 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (2.96 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (2.96 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
순도&문서
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Data Sheet (279 KB)
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SDS (481 KB)
- English - EN (481 KB)
- Français - FR (481 KB)
- Deutsch - DE (481 KB)
- Norwegian - NO (481 KB)
- Español - ES (481 KB)
- Swedish - SV (481 KB)
- Italian - IT (481 KB)
- Korean - KR (481 KB)
- Portuguese - PT (481 KB)
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Handling Instructions (2659 KB)
References
[1]. Kaur G, et al. Biological evaluation of tubulysin A: a potential anticancer and antiangiogenic natural product. Biochem J. 2006 Jun 1;396(2):235-42. [Content Brief]
[2]. Sasse F, et al. Tubulysins, new cytostatic peptides from myxobacteria acting on microtubuli. Production, isolation, physico-chemical and biological properties. J Antibiot (Tokyo). 2000 Sep;53(9):879-85. [Content Brief]
[3]. Khalil MW, et al. Mechanism of action of tubulysin, an antimitotic peptide from myxobacteria. Chembiochem. 2006 Apr;7(4):678-83. [Content Brief]
[4]. Schluep T, et al. Polymeric tubulysin-peptide nanoparticles with potent antitumor activity. Clin Cancer Res. 2009 Jan 1;15(1):181-9. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 1.1847 mL | 5.9237 mL | 11.8474 mL | 29.6184 mL |
| 5 mM | 0.2369 mL | 1.1847 mL | 2.3695 mL | 5.9237 mL | |
| 10 mM | 0.1185 mL | 0.5924 mL | 1.1847 mL | 2.9618 mL | |
| 15 mM | 0.0790 mL | 0.3949 mL | 0.7898 mL | 1.9746 mL | |
| 20 mM | 0.0592 mL | 0.2962 mL | 0.5924 mL | 1.4809 mL | |
| 25 mM | 0.0474 mL | 0.2369 mL | 0.4739 mL | 1.1847 mL | |
| 30 mM | 0.0395 mL | 0.1975 mL | 0.3949 mL | 0.9873 mL | |
| 40 mM | 0.0296 mL | 0.1481 mL | 0.2962 mL | 0.7405 mL | |
| 50 mM | 0.0237 mL | 0.1185 mL | 0.2369 mL | 0.5924 mL | |
| 60 mM | 0.0197 mL | 0.0987 mL | 0.1975 mL | 0.4936 mL | |
| 80 mM | 0.0148 mL | 0.0740 mL | 0.1481 mL | 0.3702 mL | |
| 100 mM | 0.0118 mL | 0.0592 mL | 0.1185 mL | 0.2962 mL |
Tubulysin A Related Classifications
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