Cat. No.: HY-111925

BI-749327

Name: BI-749327
Brand: MedChemExpress
SKU: HY-111925
Price: 166.00 USD
Availability: InStock
Rating: 5 (18 reviews)

18 Cited Publications

고객리뷰

Based on 18 publication(s) in Google Scholar

BI-749327 is a potent, high selectivity and orally bioavailable TRPC6 antagonist, with IC₅₀s of 13 nM, 19 nM and 15 nM for mouse, human and guinea pig TRPC6, respectively. BI-749327 is 85-fold more selective for mouse TRPC6 than TRPC3 and 42-fold versus TRPC7.

연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.

Purity : 99.95%
CAS No.: 2361241-23-6
화학식: C₂₃H₂₁F₃N₄O₂
분자량:442.43

보관:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

BI-749327 Chemical Structure

CAS No.: 2361241-23-6

사이즈

가격

재고

수량

Amount: USD 0.00

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- 제품 설명
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (18)
용액&용해도
- In Vitro
- In Vivo
순도&문서
References
Help & FAQs
Classifications
Bioz

Biological Activity

제품 설명

IC₅₀ & Target

IC50: 13 nM (mouse TRPC6), 19 nM (human TRPC6), 15 nM (guinea pig TRPC6)^[1]

All TRP Channel Isoforms :

In Vitro

BI-749327 suppresses NFAT activation in HEK293T cells expressing wild-type or gain-of-function TRPC6 mutants and blocks associated signaling and expression of prohypertrophic genes in isolated myocytes^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. .

In Vivo

BI-749327 (30 mg/kg/day; i.g.) improves left heart function, reduces volume/mass ratio, and blunts expression of profibrotic genes and interstitial fibrosis in mice subjected to sustained pressure overload^[1].
BI-749327 dose dependently reduces renal fibrosis and associated gene expression in mice with unilateral ureteral obstruction^[1].
BI-749327 has long terminal half-life (t_1/2 8.5-13.5 hours) for mice (3-30 mg/kg; p.o.)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

C57BL/6J mice^[1]
Dosage:

30 mg/kg/day
Administration:

Oral gavage
Result:

Improved left heart function, reduced volume/mass ratio, and blunted expression of profibrotic genes and interstitial fibrosis in mice subjected to sustained pressure overload.

Animal Model:

CD-1 mice^[1]
Dosage:

3 mg/kg, 10 mg/kg, 30 mg/kg
Administration:

Oral administration
Result:

t_1/2 8.5-13.5 hours

Chemical Information

CAS No. 2361241-23-6
Appearance Solid
분자량 442.43
화학식 C₂₃H₂₁F₃N₄O₂
Color White to off-white
SMILES

NC1=NN=C(C2CCN(C(C3=CC=C(OC4=CC=C(C(F)(F)F)C=C4)C=C3)=O)CC2)C=C1
선적

Room temperature in continental US; may vary elsewhere.

보관

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (18)

All In Vivo ImagingBio/Physico-chemical AssayCell Proliferation/Viability AssayRT-PCR

Sort by:

Journal Impact Factor

Cell Rep

Inducing ferroptosis to impede metastasis by inhibiting the calcium channel TRPC6. [Abstract]

2025 Nov 25;44(11):116543. PMID: 41206865

BI-749327 purchased from MedChemExpress. Usage Cited in: Cell Rep. 2025 Nov 25;44(11):116543. [Abstract]

Luciferase-labeled LM2 cells (10⁵) were injected into the tail vein of female NSG mice and the mice were treated daily with vehicle, BI-749327, liproxstatin-1, or both drugs. Mice were imaged on day 1 and day 16.

BI-749327 purchased from MedChemExpress. Usage Cited in: Cell Rep. 2025 Nov 25;44(11):116543. [Abstract]

The level of GSH levels was quantified in p27⁺ CAL-51 cells that have been pretreated with either BI-749327 (10 μM) or DMSO for 48 h and subsequently treated with IKE (10 μM) or DMSO for 24 h.

BI-749327 purchased from MedChemExpress. Usage Cited in: Cell Rep. 2025 Nov 25;44(11):116543. [Abstract]

p27⁺ CAL-51 cells were treated for 24 h with either DMSO or increasing concentrations of IKE in the presence of either BI-749327 (10 μM) or ferrostatin-1 (2 μM), or a combination of BI-749327 and ferrostatin-1. Viability was measured using the CTG assay.
Cell Rep

The calcium channel TRPC6 promotes chemotherapy-induced persistence by regulating integrin α6 mRNA splicing. [Abstract]

2023 Oct 31;42(11):113347. PMID: 37910503

BI-749327 purchased from MedChemExpress. Usage Cited in: Cell Rep. 2023 Oct 31;42(11):113347. [Abstract]

CAL-51-resistant cells were treated with vehicle control or BI-749327 (10 μM) for 24 h, and the frequency of CSCs was determined using a limiting-dilution mammosphere assay and ELDA.

BI-749327 purchased from MedChemExpress. Usage Cited in: Cell Rep. 2023 Oct 31;42(11):113347. [Abstract]

CAL-51-resistant (CAL-51-R) cells were treated with increasing concentrations of paclitaxel in combination with either DMSO or BI-749327 (10 μM) for 24 h, and cell viability was assessed.

BI-749327 purchased from MedChemExpress. Usage Cited in: Cell Rep. 2023 Oct 31;42(11):113347. [Abstract]

The organoid models were treated with either DMSO, paclitaxel (PTX; 20 nM), BI-749437 (10 μM), or PTX+BI-749327 (BI) for 96 h, and cell viability was measured.

BI-749327 purchased from MedChemExpress. Usage Cited in: Cell Rep. 2023 Oct 31;42(11):113347. [Abstract]

The PDX tumors that were treated with BI-749327 had significantly less SETD4 mRNA expression than control tumors.
Am J Physiol Cell Physiol

Mechanosensitive cation currents through TRPC6 and Piezo1 channels in human pulmonary arterial endothelial cells. [Abstract]

2022 Oct 1;323(4):C959-C973. PMID: 35968892
Function (Oxf)

PAR1-mediated Non-periodical Synchronized Calcium Oscillations in human Mesangial Cells. [Abstract]

2024 Sep 10;5(5):zqae030. PMID: 38984988
Biochim Biophys Acta Mol Basis Dis

TRPC6 N338S is a gain-of-function mutant identified in patient with doxorubicin-induced cardiotoxicity. [Abstract]

2022 Nov 1;1868(11):166505. PMID: 35882306
Cell Calcium

High-throughput assessment identifying major platelet Ca2+ entry pathways via tyrosine kinase-linked and G protein-coupled receptors. [Abstract]

2023 Jun:112:102738. PMID: 37060673
Sci Rep

Role of TRPC6 in kidney damage after acute ischemic kidney injury. [Abstract]

2022 Feb 22;12(1):3038. PMID: 35194063
Exp Neurol

TRPC6-TXNIP-NLRP3 signaling axis is involved in type 2 diabetes-associated cognitive dysfunction and the intervention of ginsenoside Rg1. [Abstract]

2026 Aug:402:115784. PMID: 41990909
Exp Neurol

Trpc6 knockout improves behavioral dysfunction and reduces Aβ production by inhibiting CN-NFAT1 signaling in T2DM mice. [Abstract]

2023 May:363:114350. PMID: 36791875
Biomedicines

Klotho Modulates Pro-Fibrotic Activities in Human Atrial Fibroblasts through Inhibition of Phospholipase C Signaling and Suppression of Store-Operated Calcium Entry. [Abstract]

2022 Jul 1;10(7):1574. PMID: 35884879
Am J Physiol Lung Cell Mol Physiol

TRPC6, a therapeutic target for pulmonary hypertension. [Abstract]

2021 Dec 1;321(6):L1161-L1182. PMID: 34704831
Behav Brain Funct

Chronic lipopolysaccharide exposure promotes cognitive impairments by activating TRPC6-AIM2 inflammasome signaling and the regulation of ginsenoside Rg1 in Trpc6-/- mice. [Abstract]

2025 Jul 22;21(1):23. PMID: 40696361
J Pharmacol Sci

2026 Sep;162(1):50-64. PMID: 42481130
Toxicon

Transient receptor potential canonical 6 is critical for chronic lipopolysaccharide exposure-induced pulmonary injury and fibrosis. [Abstract]

2026 Jun 15:276:109072. PMID: 41850440
Transfus Med

Improvement of platelet preservation by inhibition of TRPC6. [Abstract]

2023 Jun;33(3):232-243. PMID: 36746770
bioRxiv

Functional Evaluation of TRPC6 Missense Variants in Cancer Patients via Molecular Docking Analysis Compared with Patch Clamp Electrophysiology. [Abstract]

2026 Jan 2:2026.01.01.697223. PMID: 41509376
bioRxiv

2024 Mar 11.
Biomed Pharmacother

Interruption of TRPC6-NFATC1 signaling inhibits NADPH oxidase 4 and VSMCs phenotypic switch in intracranial aneurysm. [Abstract]

2023 May:161:114480. PMID: 37002575

용액&용해도

In Vitro:

DMSO : 31.25 mg/mL (70.63 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.2602 mL

11.3012 mL

22.6024 mL

5 mM

0.4520 mL

2.2602 mL

4.5205 mL

10 mM

0.2260 mL

1.1301 mL

2.2602 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

몰농도 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

농도 희석 계산기

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (4.70 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (4.70 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (4.70 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

순도&문서

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (275 KB)
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Lin B L, et al. In vivo selective inhibition of TRPC6 by antagonist BI 749327 ameliorates fibrosis and dysfunction in cardiac and renal disease. Proc Natl Acad Sci U S A. 2019 May 14;116(20):10156-10161. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.2602 mL	11.3012 mL	22.6024 mL	56.5061 mL
	5 mM	0.4520 mL	2.2602 mL	4.5205 mL	11.3012 mL
	10 mM	0.2260 mL	1.1301 mL	2.2602 mL	5.6506 mL
	15 mM	0.1507 mL	0.7534 mL	1.5068 mL	3.7671 mL
	20 mM	0.1130 mL	0.5651 mL	1.1301 mL	2.8253 mL
	25 mM	0.0904 mL	0.4520 mL	0.9041 mL	2.2602 mL
	30 mM	0.0753 mL	0.3767 mL	0.7534 mL	1.8835 mL
	40 mM	0.0565 mL	0.2825 mL	0.5651 mL	1.4127 mL
	50 mM	0.0452 mL	0.2260 mL	0.4520 mL	1.1301 mL
	60 mM	0.0377 mL	0.1884 mL	0.3767 mL	0.9418 mL

Bioz

Keywords

BI-749327

18 Cited Publications

100 mg 견적 받기

Subscribe to the

MedChemExpress

Newsletter

상품명:
Cat. No.:
수량:
MCE Japan Authorized Agent:

상품명:
CAS No.:
Species:
Purity:
수량:

BI-749327

Publications Citing Use of MedChemExpress (MCE) BI-749327

All TRP Channel Isoforms