2. Metabolic Enzyme/Protease
  3. MMP
  4. MMP-2/MMP-9 Substrate

MMP-2/MMP-9 Substrate  (Synonyms: Ac-Pro-Leu-Gly-[(S)-2-mercapto-4-methyl-pentanoyl]-Leu-Gly-OEt)

Cat. No.: HY-P10143 Purity: 98.48%
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Handling Instructions Technical Support

MMP-2/MMP-9 Substrate (Ac-Pro-Leu-Gly-[(S)-2-mercapto-4-methyl-pentanoyl]-Leu-Gly-OEt) is a synthetic chromogenic polypeptide substrate whose core structure mimics the cleavage sites of MMP-2 and MMP-9 (gelatinase A and B) in collagen. After being hydrolyzed by collagenase, MMP-2/MMP-9 Substrate reacts with 4,4'-dithiodipyridine or Ellman's Reagent via its thiol fragment to produce a product with ultraviolet absorption properties.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

MMP-2/MMP-9 Substrate

MMP-2/MMP-9 Substrate Chemical Structure

CAS No. : 99828-55-4

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MMP-2/MMP-9 Substrate Related Antibodies

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MMP-1 MMP-2 MMP-3 MMP-8 MMP-9 MMP-13 MMP-14 MMP-17 ADAM10 ADAM17 MMP-7 MMP-12 MMP-10 MMP ADAM8 MMP-19

  • Biological Activity

  • Purity & Documentation

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  • Customer Review

Description

MMP-2/MMP-9 Substrate (Ac-Pro-Leu-Gly-[(S)-2-mercapto-4-methyl-pentanoyl]-Leu-Gly-OEt) is a synthetic chromogenic polypeptide substrate whose core structure mimics the cleavage sites of MMP-2 and MMP-9 (gelatinase A and B) in collagen. After being hydrolyzed by collagenase, MMP-2/MMP-9 Substrate reacts with 4,4'-dithiodipyridine or Ellman's Reagent via its thiol fragment to produce a product with ultraviolet absorption properties[1][2].

IC50 & Target[1]

MMP-2

 

MMP-9

 

In Vitro

MMP-2/MMP-9 Substrate can be hydrolyzed by human skin fibroblast collagenase, human skin gelatinase, elastase, kallikrein, α-chymotrypsin, urokinase, trypsin, carboxypeptidase b, and bacterial collagenase[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

MMP-2/MMP-9 Substrate Related Antibodies
Molecular Weight

655.85

Formula

C31H53N5O8S
CAS No.
Appearance

Solid

Color

White to off-white

Sequence

Ac-Pro-Leu-{Gly-[(S)-2-mercapto-4-methyl-pentanoyl]}-Leu-{Gly-OEt}
Sequence Shortening

Ac-PL-{Gly-[(S)-2-mercapto-4-methyl-pentanoyl]}-L-{Gly-OEt}
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (152.47 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.5247 mL 7.6237 mL 15.2474 mL
5 mM 0.3049 mL 1.5247 mL 3.0495 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.5247 mL 7.6237 mL 15.2474 mL 38.1185 mL
5 mM 0.3049 mL 1.5247 mL 3.0495 mL 7.6237 mL
10 mM 0.1525 mL 0.7624 mL 1.5247 mL 3.8118 mL
15 mM 0.1016 mL 0.5082 mL 1.0165 mL 2.5412 mL
20 mM 0.0762 mL 0.3812 mL 0.7624 mL 1.9059 mL
25 mM 0.0610 mL 0.3049 mL 0.6099 mL 1.5247 mL
30 mM 0.0508 mL 0.2541 mL 0.5082 mL 1.2706 mL
40 mM 0.0381 mL 0.1906 mL 0.3812 mL 0.9530 mL
50 mM 0.0305 mL 0.1525 mL 0.3049 mL 0.7624 mL
60 mM 0.0254 mL 0.1271 mL 0.2541 mL 0.6353 mL
80 mM 0.0191 mL 0.0953 mL 0.1906 mL 0.4765 mL
100 mM 0.0152 mL 0.0762 mL 0.1525 mL 0.3812 mL
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MMP-2/MMP-9 Substrate Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

MMP-2/MMP-9 Substrate99828-55-4Ac-Pro-Leu-Gly-[(S)-2-mercapto-4-methyl-pentanoyl]-Leu-Gly-OEtMMPMatrix metalloproteinaseskallikreinhuman skin fibroblast collagenasevertebrate collagenasecarboxypeptidase bhuman skin gelatinasetrypsinelastaseα-chymotrypsinbacterial collagenaseurokinaseInhibitorinhibitorinhibit

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