N1,N11-Diethylnorspermine tetrahydrochloride
Based on 7 publication(s) in Google Scholar
N1, N11-Diethylnorspermine tetrahydrochloride (DENSPM tetrahydrochloride) is a potent anticancer agent. N1,N11-Diethylnorspermine tetrahydrochloride activates polyamine catabolism and downregulates mTOR protein. N1,N11-Diethylnorspermine tetrahydrochloride induces the release of cytochrome c from mitochondria, resulting in activation of caspase 3. N1,N11-Diethylnorspermine tetrahydrochloride kills glioblastoma multiforme (GBM) through induction of SSAT (spermidine/spermine N1-acetyltransferase) coupled with H2O2 production.
For research use only. We do not sell to patients.
- Purity : 99.82%
- CAS No.: 156886-85-0
- Formula: C13H36Cl4N4
- Molecular Weight:390.26
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) N1,N11-Diethylnorspermine tetrahydrochloride
More- Nature. 2026 Jul;655(8121):240-250. [Abstract]
- Immunity. 2023 Nov 14;56(11):2508-2522.e6. [Abstract]
- Nat Commun. 2025 May 27;16(1):4919. [Abstract]
- J Clin Invest. 2026 Jun 1;136(11):e201460. [Abstract]
- Proc Natl Acad Sci U S A. 2024 Mar 26;121(13):e2319429121. [Abstract]
- PLoS One. 2025 Feb 12;20(2):e0308049. [Abstract]
- Seoul National University. 2026.
Customer Validation & Images
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Cell Imaging/Staining
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RT-PCR
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ELISA
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WB
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RT-PCR
All Caspase Isoforms
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Biological Activity
Description
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Caspase 3 |
In Vivo
N1,N11-Diethylnorspermine (120-360 mg/kg, s.c., 4 days) tetrahydrochloride results in prolonged inhibition of tumor growth and long-term tumor regressions in nude athymic mice with MALME-3M melanoma xenografts[5].
N1,N11-Diethylnorspermine (12.5-50 mg/kg, i.v., 5 days) tetrahydrochloride is well tolerated and induces no physical signs in CD rats[6].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 156886-85-0
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Appearance Solid
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Molecular Weight 390.26
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Formula C13H36Cl4N4
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Color White to off-white
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SMILES
CCNCCCNCCCNCCCNCC.[H]Cl.[H]Cl.[H]Cl.[H]Cl
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Synonyms
Diethylnorspermine tetrahydrochloride; DENSPM tetrahydrochloride; BENSPM tetrahydrochloride
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (7)
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Journal Impact Factor
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Most Recent
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Nature
2026 Jul;655(8121):240-250. PMID: 42236947 -
Immunity
Polyamine metabolism controls B-to-Z DNA transition to orchestrate DNA sensor cGAS activity. [Abstract]2023 Nov 14;56(11):2508-2522.e6. PMID: 37848037
N1,N11-Diethylnorspermine tetrahydrochloride purchased from MedChemExpress. Usage Cited in: Immunity. 2023 Nov 14;56(11):2508-2522.e6. [Abstract]
N1, N11-Diethylnorspermine tetrahydrochloride (DENSpm) (10 μM; 24 h) alleviated HSV-1-infection- and ISD transfection-induced Z-DNA accumulation in PMs.
N1,N11-Diethylnorspermine tetrahydrochloride purchased from MedChemExpress. Usage Cited in: Immunity. 2023 Nov 14;56(11):2508-2522.e6. [Abstract]
N1, N11-Diethylnorspermine tetrahydrochloride (DENSpm) (1-20 μM; 24 h) promoted HSV-1 infection-induced expression of Ifnb in PMs in a dose-dependent manner.
N1,N11-Diethylnorspermine tetrahydrochloride purchased from MedChemExpress. Usage Cited in: Immunity. 2023 Nov 14;56(11):2508-2522.e6. [Abstract]
N1, N11-Diethylnorspermine tetrahydrochloride (DENSpm) (10 μM; 24 h) promoted HSV-1-infection-induced expression of IFN-βin PMs.
N1,N11-Diethylnorspermine tetrahydrochloride purchased from MedChemExpress. Usage Cited in: Immunity. 2023 Nov 14;56(11):2508-2522.e6. [Abstract]
N1, N11-Diethylnorspermine tetrahydrochloride (DENSpm) (1-20 μM; 24 h) promoted HSV-1-infection-induced phosphorylation of TBK1 and IRF3 in a dose-dependent manner in PMs.
N1,N11-Diethylnorspermine tetrahydrochloride purchased from MedChemExpress. Usage Cited in: Immunity. 2023 Nov 14;56(11):2508-2522.e6. [Abstract]
N1, N11-Diethylnorspermine tetrahydrochloride (DENSpm) (2 nM; i.p.; once every 2 d) enhanced HSV-1-infection-induced Ifnb mRNA expression in the brain and spleen of C57BL/6 mice.
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Nat Commun
Ferroptosis-activating metabolite acrolein antagonizes necroptosis and anti-cancer therapeutics. [Abstract]2025 May 27;16(1):4919. PMID: 40425585 -
J Clin Invest
Polyamine sequestration of 2'3'-cGAMP constrains intercellular transmission and STING engagement to subvert antitumor immunity. [Abstract]2026 Jun 1;136(11):e201460. PMID: 42222888 -
Proc Natl Acad Sci U S A
Activation of polyamine catabolism promotes glutamine metabolism and creates a targetable vulnerability in lung cancer. [Abstract]2024 Mar 26;121(13):e2319429121. PMID: 38513095 -
PLoS One
Development of a reliable, sensitive, and convenient assay for the discovery of new eIF5A hypusination inhibitors. [Abstract]2025 Feb 12;20(2):e0308049. PMID: 39937781 -
Solvent & Solubility
In Vitro:
H2O : 100 mg/mL (256.24 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 100 mg/mL (256.24 mM); Clear solution; Need ultrasonic
Purity & Documentation
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Data Sheet (270 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Schipper RG, et al. Antitumor activity of the polyamine analog N(1), N(11)-diethylnorspermine against human prostate carcinoma cells. Prostate. 2000 Sep 1;44(4):313-21. [Content Brief]
[2]. Holst CM, et al. Molecular mechanisms underlying N1, N11-diethylnorspermine-induced apoptosis in a human breast cancer cell line. Anticancer Drugs. 2008 Oct;19(9):871-83. [Content Brief]
[3]. Jiang R, et al. Activation of polyamine catabolism by N1,N11-diethylnorspermine leads to cell death in glioblastoma. Int J Oncol. 2007 Aug;31(2):431-40. [Content Brief]
[4]. Jiang R, et al. Activation of polyamine catabolism by N1, N11-diethylnorspermine alters the cellular localization of mTOR and downregulates mTOR protein level in glioblastoma cells. Cancer Biol Ther. 2007 Oct;6(10):1644-8. [Content Brief]
[5]. Bernacki RJ, et al. Preclinical antitumor efficacy of the polyamine analogue N1, N11-diethylnorspermine administered by multiple injection or continuous infusion. Clin Cancer Res. 1995 Aug;1(8):847-57. [Content Brief]
[6]. Kanter PM, et al. Preclinical toxicologic evaluation of DENSPM (N1,N11-diethylnorspermine) in rats and dogs. Anticancer Drugs. 1994 Aug;5(4):448-56. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| H2O | 1 mM | 2.5624 mL | 12.8120 mL | 25.6239 mL | 64.0599 mL |
| 5 mM | 0.5125 mL | 2.5624 mL | 5.1248 mL | 12.8120 mL | |
| 10 mM | 0.2562 mL | 1.2812 mL | 2.5624 mL | 6.4060 mL | |
| 15 mM | 0.1708 mL | 0.8541 mL | 1.7083 mL | 4.2707 mL | |
| 20 mM | 0.1281 mL | 0.6406 mL | 1.2812 mL | 3.2030 mL | |
| 25 mM | 0.1025 mL | 0.5125 mL | 1.0250 mL | 2.5624 mL | |
| 30 mM | 0.0854 mL | 0.4271 mL | 0.8541 mL | 2.1353 mL | |
| 40 mM | 0.0641 mL | 0.3203 mL | 0.6406 mL | 1.6015 mL | |
| 50 mM | 0.0512 mL | 0.2562 mL | 0.5125 mL | 1.2812 mL | |
| 60 mM | 0.0427 mL | 0.2135 mL | 0.4271 mL | 1.0677 mL | |
| 80 mM | 0.0320 mL | 0.1601 mL | 0.3203 mL | 0.8007 mL | |
| 100 mM | 0.0256 mL | 0.1281 mL | 0.2562 mL | 0.6406 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
N1,N11-Diethylnorspermine tetrahydrochloride Related Classifications
HY-13610A Related Classifications
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