2. Apoptosis
  3. Caspase Apoptosis
  4. PETCM

PETCM is an activator of caspase-3 and acts as an cytochrome c (cyto c)-dependent manner. PETCM promotes Apaf-1 oligomerization and induces cell apoptosis in HeLa cells.

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PETCM Chemical Structure

PETCM Chemical Structure

CAS No. : 10129-56-3

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10 mM * 1 mL in DMSO
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Customer Review

Based on 2 publication(s) in Google Scholar

PETCM Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE PETCM

WB

    PETCM purchased from MedChemExpress. Usage Cited in: Diabetes. 2022 Oct 18;db220231.  [Abstract]

    PETCM (10 μM) activates apoptosis in HG-PA–treated HRGEC transfected with L-Scr and HG-PA–treated HRGEC transfected with L-shFgf13.

    View All Caspase Isoform Specific Products:

    View All Isoforms
    Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    PETCM is an activator of caspase-3 and acts as an cytochrome c (cyto c)-dependent manner. PETCM promotes Apaf-1 oligomerization and induces cell apoptosis in HeLa cells[1][2].

    IC50 & Target[1]

    Caspase 3

     

    In Vitro

    PETCM (0.1-0.5 mM) stimulates caspase-3 activity (DEVD activity) of HeLa S-100 in a dose-dependent manner.And 0.2 mM PETCM is more efficient in activating caspase-3 than 1 mM dATP[1].
    PETCM (0.2 mM; 1 hour) drives apoptosome formation. Apaf-1 in a normal HeLa cell S-100 fraction is in an inactive monomeric form. After 1 mM dATP, most of the Apaf-1 shifted to a size of 1 million daltons. After the S-100 fraction with 0.2 mM PETCM, Apaf-1 exhibits a similar shift. And the efficiency of apoptosome formation was better with 0.2 mM PETCM[1].
    PETCM (0.2 mM; 1 hour) can antagonize the inhibitory activity of ProT reduced caspase-3 activation in in HeLa cells[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    PETCM Related Antibodies

    Western Blot Analysis[1]

    Cell Line: HeLa cells
    Concentration: 0.2 mM
    Incubation Time: 1 hour
    Result: Increased apoptosome formation.
    Molecular Weight

    240.51

    Formula

    C8H8Cl3NO
    CAS No.
    Appearance

    Solid

    Color

    Light yellow to light brown

    SMILES

    OC(C(Cl)(Cl)Cl)CC1=CC=NC=C1
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (415.78 mM; ultrasonic and heat to 40°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 4.1578 mL 20.7892 mL 41.5783 mL
    5 mM 0.8316 mL 4.1578 mL 8.3157 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (10.39 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (10.39 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

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    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
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    % 
    +
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    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.36%

    COA (248 KB) HNMR (251 KB) LCMS (266 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 4.1578 mL 20.7892 mL 41.5783 mL 103.9458 mL
    5 mM 0.8316 mL 4.1578 mL 8.3157 mL 20.7892 mL
    10 mM 0.4158 mL 2.0789 mL 4.1578 mL 10.3946 mL
    15 mM 0.2772 mL 1.3859 mL 2.7719 mL 6.9297 mL
    20 mM 0.2079 mL 1.0395 mL 2.0789 mL 5.1973 mL
    25 mM 0.1663 mL 0.8316 mL 1.6631 mL 4.1578 mL
    30 mM 0.1386 mL 0.6930 mL 1.3859 mL 3.4649 mL
    40 mM 0.1039 mL 0.5197 mL 1.0395 mL 2.5986 mL
    50 mM 0.0832 mL 0.4158 mL 0.8316 mL 2.0789 mL
    60 mM 0.0693 mL 0.3465 mL 0.6930 mL 1.7324 mL
    80 mM 0.0520 mL 0.2599 mL 0.5197 mL 1.2993 mL
    100 mM 0.0416 mL 0.2079 mL 0.4158 mL 1.0395 mL
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    PETCM Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    PETCM10129-56-3CaspaseApoptosisactivatorapaf-1helacellcancerapoptosisdevdInhibitorinhibitorinhibit

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