PROTAC BRD9 Degrader-6
Based on 1 Customer Validation
PROTAC BRD9 Degrader-6 (Compound D1) is a racemic PROTAC degrader that recruits cereblon to induce targeted degradation of BRD9. PROTAC BRD9 Degrader-6 has an IC50 of 0.13 nM in SYO-1 cells. It can be used in research on BAF complex-related diseases, such as cancer, infections and synovial sarcoma.
(Pink: BRD9 ligand (HY-49393); Blue: Cereblon ligand (HY-W244657); Black: linker).
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza : 98.19%
- No. CAS: 2676211-62-2
- Fòrmula: C47H56N8O6
- Peso molecular:829.00
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Protocolo
- Pureza y Documentación
- Referencias
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Help & FAQs
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Actividad biológica
Descripciòn
IC50 & Target
[1]|
BRD9 |
In Vitro
PROTAC BRD9 Degrader-6 (Comp D1) potently degrades BRD9-NanoLuc in SYO-1 cells, with an IC50 of 0.13 nM[1].
PROTAC BRD9 Degrader-6 (0.01-1.1 nM; 1-24 h) induces concentration- and time-dependent degradation of BRD9 protein in SYO-1 cells, with a gradual increase in degradation level observed after 1 h, 6 h, and 24 h of incubation[1].
PROTAC BRD9 Degrader-6 (0.5-2 h) rapidly depletes BRD9 protein in SYO-1 cells, with nearly complete degradation observable within 0.5 h of treatment[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:SYO-1 synovial sarcoma cells
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Concentration:0.01 nM; 0.12 nM; 1.1 nM
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Incubation Time:1 h; 6 h; 24 h
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Result:Showed concentration-dependent reduction of BRD9 protein levels relative to DMSO control at 1 h incubation.
Further reduced BRD9 protein levels across all tested concentrations at 6 h incubation.
Showed marked concentration-dependent reduction of BRD9 protein levels at 24 h incubation.
Chemical Information
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No. CAS 2676211-62-2
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Appearance Solid
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Peso molecular 829.00
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Fòrmula C47H56N8O6
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Color White to off-white
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SMILES
O=C1C2=C(C(C3=CC(OC)=C(CN4CCC(CC4)CN5CCC6(CC5)CN(C6)C7=CC=C8C(C(N(C9C(NC(CC9)=O)=O)C8)=O)=C7)C(OC)=C3)=CN1C)C=C(N%10CCC%10)N=C2
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvente y solubilidad
In Vitro:
DMSO : 100 mg/mL (120.63 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (3.02 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocolo
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
Pureza y Documentación
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Ficha de datos (272 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 1.2063 mL | 6.0314 mL | 12.0627 mL | 30.1568 mL |
| 5 mM | 0.2413 mL | 1.2063 mL | 2.4125 mL | 6.0314 mL | |
| 10 mM | 0.1206 mL | 0.6031 mL | 1.2063 mL | 3.0157 mL | |
| 15 mM | 0.0804 mL | 0.4021 mL | 0.8042 mL | 2.0105 mL | |
| 20 mM | 0.0603 mL | 0.3016 mL | 0.6031 mL | 1.5078 mL | |
| 25 mM | 0.0483 mL | 0.2413 mL | 0.4825 mL | 1.2063 mL | |
| 30 mM | 0.0402 mL | 0.2010 mL | 0.4021 mL | 1.0052 mL | |
| 40 mM | 0.0302 mL | 0.1508 mL | 0.3016 mL | 0.7539 mL | |
| 50 mM | 0.0241 mL | 0.1206 mL | 0.2413 mL | 0.6031 mL | |
| 60 mM | 0.0201 mL | 0.1005 mL | 0.2010 mL | 0.5026 mL | |
| 80 mM | 0.0151 mL | 0.0754 mL | 0.1508 mL | 0.3770 mL | |
| 100 mM | 0.0121 mL | 0.0603 mL | 0.1206 mL | 0.3016 mL |
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