Romidepsin (GMP) (FK 228 (GMP)) is Romidepsin (HY-15149) produced by using GMP guidelines. GMP small molecules works appropriately as an auxiliary reagent for cell therapy manufacture. Romidepsin (FK 228) is a Histone deacetylase (HDAC) inhibitor with anti-tumor activities. Romidepsin (FK 228) inhibits HDAC1, HDAC2, HDAC4, and HDAC6 with IC50s of 36 nM, 47 nM, 510 nM and 1.4 μM, respectively. Romidepsin (FK 228) is produced by Chromobacterium violaceum, induces cell G2/M phase arrest and apoptosis.
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- Fòrmula: C24H36N4O6S2
- Peso molecular:540.70
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Almacenamiento:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Actividad biológica
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Chemical Information
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Help & FAQs
Actividad biológica
Descripciòn
In Vitro
Romidepsin (GMP) (0-72 hours; 0-80 nM) inhibits proliferation of HCC cells in dose-dependent manner[2]. Romidepsin (GMP) (0-48 hours; 0-60 nM) leads to a time- and dose-dependent induction of cell cycle arrest in the G2/M phase in HCC cells[2]. Romidepsin (GMP) (0-48 hours; 0-60 nM) promotesapoptosis in HCC cells, increases c-caspase-3, c-caspase-9, and c-PARP protein expression[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HCC cells
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Concentration:0 nM; 10 nM; 20 nM; 30 nM; 40 nM; 50 nM; 60 nM; 70 nM; 80 nM
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Incubation Time:0 hours; 12 hours; 24 hours; 48 hours; 72 hours
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Result:Inhibited HCC cells proliferation.
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Cell Line:HCC cells
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Concentration:0 nM; 15 nM; 30 nM; 60 nM
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Incubation Time:12 hours; 24 hours; 48 hours
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Result:Caused a G2/M arrest.
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Cell Line:HCC cells
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Concentration:0 nM; 15 nM; 30 nM; 60 nM
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Incubation Time:12 hours; 24 hours; 48 hours
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Result:Increaesd c-caspase-3, c-caspase-9, and c-PARP expression in HCC cells.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Nude mice with Huh7 cells[2]
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Dosage:0.5 and 1 mg/kg
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Administration:Intraperitoneal injection; 0.5 and 1 mg/kg; every 3 day; 21 days
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Result:Suppressed tumor growth in mouse xenograft models.
Chemical Information
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Peso molecular 540.70
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Fòrmula C24H36N4O6S2
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SMILES
C/C=C(NC([C@@H](CSSCC/C=C/[C@@H](O1)CC2=O)NC([C@@H](C(C)C)N2)=O)=O)/C(N[C@@H](C(C)C)C1=O)=O
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Synonyms
FK 228 (GMP); FR 901228 (GMP); NSC 630176 (GMP)
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureza y Documentación
Referencias
[1]. Furumai R, et al. FK228 (depsipeptide) as a natural prodrug that inhibits class I histone deacetylases. Cancer Res. 2002 Sep 1;62(17):4916-21. [Content Brief]
[2]. Sun WJ, et al. Romidepsin induces G2/M phase arrest via Erk/cdc25C/cdc2/cyclinB pathway and apoptosis induction through JNK/c-Jun/caspase3 pathway in hepatocellular carcinoma cells. Biochem Pharmacol. 2017 Mar 1;127:90-100. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Romidepsin (GMP) Related Classifications
HY-15149G Related Classifications
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