Referencia número: HY-N125722

Venturicidin A

Name: Venturicidin A
Brand: MedChemExpress
SKU: HY-N125722
Price: 416.00 USD
Availability: InStock
Rating: 5 (1 reviews)

(Synonyms: Aabomycin A1)

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Venturicidin A (Aabomycin A1) is a natural product derived from Streptomyces, possessing antifungal, anti-Trypanosoma brucei, anti-Leishmania donovani, and ATP synthase inhibitory activities. Venturicidin A acts as an antibiotic adjuvant, promoting the uptake of aminoglycoside antibiotics and enhancing bactericidal efficacy. Venturicidin A inhibits ATP synthesis by blocking proton translocation through the F_O subunit. In bloodstream-form Trypanosoma brucei, Venturicidin A causes collapse of the mitochondrial membrane potential; in fungi, it disrupts cell membrane integrity, induces leakage of cytoplasmic contents, elevates ROS levels, and downregulates the expression of fungal pathogenicity-related genes. Venturicidin A exhibits antifungal activity against Botrytis cinerea. Venturicidin A is used in research on bacterial infections, trypanosome/leishmania infections, and gray mold disease.

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Pureza : 96.60%
No. CAS: 33538-71-5
Fòrmula: C₄₁H₆₇NO₁₁
Peso molecular:749.97

Almacenamiento:

Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month

Venturicidin A Chemical Structure

No. CAS: 33538-71-5

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Actividad biológica
- Descripciòn
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Solvente y solubilidad
- In Vitro
Pureza y Documentación
Referencias
Help & FAQs
Classifications
Bioz

Actividad biológica

Descripciòn

IC₅₀ & Target

Aminoglycoside

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Cellular Effect

Cell Line	Type	Value	Description	References
B16	IC₅₀	12 μM Compound: 4	Cytotoxicity against mouse B16 cells assessed as inhibition of cell growth measured after 72 hrs by Celltiter-Glo assay Cytotoxicity against mouse B16 cells assessed as inhibition of cell growth measured after 72 hrs by Celltiter-Glo assay	[PMID: 33356258]
Hep 3B2	IC₅₀	15 μM Compound: 4	Cytotoxicity against human Hep3B cells assessed as inhibition of cell growth measured after 72 hrs by Celltiter-Glo assay Cytotoxicity against human Hep3B cells assessed as inhibition of cell growth measured after 72 hrs by Celltiter-Glo assay	[PMID: 33356258]
K562	IC₅₀	5.8 μM Compound: 4	Cytotoxicity against human K562 cells assessed as inhibition of cell growth measured after 72 hrs by Celltiter-Glo assay Cytotoxicity against human K562 cells assessed as inhibition of cell growth measured after 72 hrs by Celltiter-Glo assay	[PMID: 33356258]
MDA-MB-468	IC₅₀	8.3 μM Compound: 4	Cytotoxicity against human MDA-MB-468 cells assessed as inhibition of cell growth measured after 72 hrs by Celltiter-Glo assay Cytotoxicity against human MDA-MB-468 cells assessed as inhibition of cell growth measured after 72 hrs by Celltiter-Glo assay	[PMID: 33356258]
MV4-11	IC₅₀	0.09 μM Compound: 4	Cytotoxicity against human MV4-11 cells assessed as inhibition of cell growth measured after 72 hrs by Celltiter-Glo assay Cytotoxicity against human MV4-11 cells assessed as inhibition of cell growth measured after 72 hrs by Celltiter-Glo assay	[PMID: 33356258]

In Vitro

Bacterial InfectionCytokines & ChemokinesFungal InfectionOxidative StressOthersMitochondrial FunctionParasitic Infection

Bacterial Infection

Cytokines & Chemokines

Venturicidin A (8-16 μg/mL; 24 h) enhances the activity of Gentamicin (HY-A0276A) against MDR isolates of MRSA, VRE, P. aeruginosa, A. baumannii, E. coli, and K. pneumoniae, with fractional inhibitory concentration indices below 0.5, but shows no enhancement against isolates carrying the ArmA ribosomal methyltransferase^[1].

Fungal Infection

Oxidative Stress

Venturicidin A in combination with Gentamicin (16-32 μg/mL; 0-24 h) exhibits rapid bactericidal activity against aminoglycoside-resistant MRSA C1014, reducing viable cells by >3 log CFU/mL within 4 h^[1].

Others

Venturicidin A does not inhibit the bifunctional aminoglycoside-modifying enzyme AAC (6′)-Ie-APH (2′′)-Ia, but instead acts as a Type 1b adjuvant through an indirect mechanism to enhance the effects of aminoglycosides^[1].
Venturicidin A (48 h) exhibits in vitro toxicity against human embryonic kidney (HEK) cells with an IC₅₀ of 31 μg/mL^[1].

Bacterial Infection

Mitochondrial Function

Venturicidin A is an inhibitor of ATP synthesis in MRSA C1014, depleting intracellular ATP levels by approximately 10-fold; and it induces membrane depolarization in MRSA C1014 in a dose-dependent manner^[1].

Bacterial Infection

Venturicidin A (VentA) (1.56-50 μg/mL; 5 days) effectively inhibits the mycelial growth of Botrytis cinerea on PDA medium, with an EC₅₀ of 1.08 μg/mL^[2].
Venturicidin A (1.08-12.5 μg/mL; 6-24 h) significantly inhibits Botrytis cinerea spore germination and germ tube elongation in a concentration-dependent manner; it also disrupts the morphology of spores and hyphae; it disrupts the integrity of the hyphal plasma membrane, leading to loss of cell viability^[2].
Venturicidin A (1.08-12.5 μg/mL; 12-24 hpi) impairs the penetration ability of Botrytis cinerea into onion epidermal cells^[2].

Oxidative Stress

Bacterial Infection

Venturicidin A (1.08-12.5 μg/mL; 2-10 h) severely disrupts the cell membrane integrity of Botrytis cinerea, leading to massive leakage of nucleic acids and proteins; it also increases MDA content in mycelia, indicating lipid peroxidation and membrane damage^[2].
Venturicidin A (1.08-12.5 μg/mL; 24 h) induces ROS accumulation in Botrytis cinerea hyphae; downregulates the expression of pathogenicity-related genes; and upregulates the expression of NADPH oxidase subunit genes^[2].

Fungal Infection

Venturicidin A (1.08-12.5 μg/mL; spray; once; 24 h before inoculation) inhibits spore germination and mycelial growth of Botrytis cinerea, disrupts fungal cell membrane integrity, induces ROS burst, and downregulates pathogenesis-related genes; it exhibits both protective and curative activity against tomato gray mold^[2].

Parasitic Infection

Venturicidin A exhibits high activity against Trypanosoma brucei brucei 2T1 BSF, Trypanosoma brucei rhodesiense BSF, and Leishmania donovani promastigotes, axenic amastigotes, and intracellular amastigotes, but shows markedly reduced activity against Trypanosoma evansi STIB806 BSF, with IC₅₀ values of 21.49 nM, 5.05 nM, 24.10 nM, 2.28 nM, 267.6 nM, and 5260 nM, respectively^[3].

Parasitic Infection

Mitochondrial Function

Venturicidin A (21-172 nM; 24 h) treatment of Trypanosoma brucei brucei 2T1 bloodstream forms leads to collapse of the mitochondrial membrane potential and induces loss of kDNA^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Real Time qPCR^[2]

Cell Line:

Botrytis cinerea hyphae
Concentration:

1.08, 6.25, 12.5 μg/mL
Incubation Time:

24 h
Result:

Downregulated the expression of pathogenicity-related genes; and upregulated the expression of NADPH oxidase subunit genes.

Immunofluorescence^[3]

Cell Line:

Trypanosoma brucei brucei 2T1 bloodstream forms
Concentration:

21, 107, 172 nM
Incubation Time:

24 h
Result:

Induced a loss of kDNA in approximately 40% of cells at 21 nM and 107 nM.
Induced a loss of kDNA in up to 50% of cells at 172 nM.
Induced abnormal cell division with an abnormal number of kDNA (1K2N) at all tested concentrations.

Related Protocols :

Chemical Information

No. CAS 33538-71-5
Appearance Solid
Peso molecular 749.97
Fòrmula C₄₁H₆₇NO₁₁
Color White to off-white
SMILES

CC1=CC[C@](O)(C2)O[C@]1([H])/C(C)=C\CCC[C@](O[C@@]3([H])C[C@H]([C@H](O)[C@@H](C)O3)OC(N)=O)([H])/C=C/[C@H](C)C[C@@H](C)[C@]([C@H](C)C[C@@H](C)[C@H](O)[C@H](C)C(CC)=O)([H])OC2=O
Synonyms

Aabomycin A1
Structure Classification
- Antibiotics
- Macrolide Antibiotics
Initial Source
- Microorganism
actinomycetes
Envío

Room temperature in continental US; may vary elsewhere.

Almacenamiento

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvente y solubilidad

In Vitro:

DMSO : 8 mg/mL (10.67 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.3334 mL

6.6669 mL

13.3339 mL

5 mM

0.2667 mL

1.3334 mL

2.6668 mL

10 mM

0.1333 mL

0.6667 mL

1.3334 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Calculadora de molaridad

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculadora de dilución

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

Pureza y Documentación

Select Batch:

Pureza: 99.98%

Assay: 99.98%

ee.: 99.98%

Ficha de datos (291 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instrucciones de manejo (2659 KB)

Referencias

[1]. Yarlagadda V, et al. Venturicidin A, A Membrane-active Natural Product Inhibitor of ATP synthase Potentiates Aminoglycoside Antibiotics. Scientific reports. 2020 May 18;10(1):8134. [Content Brief]

[2]. Hu L, et al. Antifungal activity mechanisms of venturicidin A against Botrytis cinerea contributes to the control of gray mould. Pest management science. 2025 Feb;81(2):1113-1126.

[3]. Hauser DA, et al. Venturicidin A affects the mitochondrial membrane potential and induces kDNA loss in Trypanosoma brucei. Antimicrob Agents Chemother. 2024 Jul 9;68(7):e0167123.

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.3334 mL	6.6669 mL	13.3339 mL	33.3347 mL
	5 mM	0.2667 mL	1.3334 mL	2.6668 mL	6.6669 mL
	10 mM	0.1333 mL	0.6667 mL	1.3334 mL	3.3335 mL

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Venturicidin A

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