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Cat. No.: HY-N1710

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28-Deoxonimbolide

28-Deoxonimbolide is a Nimbin (HY-N3187) type limonoid, that can be isolated from Azadirachta indica seed extracts. 28-Deoxonimbolide shows anticancer activity. 28-Deoxonimbolide induces apoptotic cell death in HL60 cells via both the mitochondrial- and the death receptor-mediated pathways.

For research use only. We do not sell to patients.

CAS No.: 126005-94-5
Formula: C₂₇H₃₂O₆
Molecular Weight:452.54

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (280 KB)

28-Deoxonimbolide Chemical Structure

CAS No.: 126005-94-5

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Protocols (4)
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

Caspase-8

Caspase-9

Caspase-3

All Caspase Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	9.3 μM Compound: 28	Cytotoxicity against human A549 cells assessed as cell viability after 48 hrs by MTT assay Cytotoxicity against human A549 cells assessed as cell viability after 48 hrs by MTT assay	[PMID: 21381696]
AZ-521 cell line	IC₅₀	2.4 μM Compound: 28	Cytotoxicity against human AZ-521 cells assessed as cell viability after 48 hrs by MTT assay Cytotoxicity against human AZ-521 cells assessed as cell viability after 48 hrs by MTT assay	[PMID: 21381696]
BC1 cell line	ED₅₀	1.34 μg/mL Compound: 2	Cytotoxicity against human BC1 cells after 72 hrs Cytotoxicity against human BC1 cells after 72 hrs	[PMID: 2614419]
Col2	ED₅₀	1.81 μg/mL Compound: 2	Cytotoxicity against human Col2 cells after 72 hrs Cytotoxicity against human Col2 cells after 72 hrs	[PMID: 2614419]
HL-60	IC₅₀	2.7 μM Compound: 28	Cytotoxicity against human HL60 cells assessed as cell viability after 48 hrs by MTT assay Cytotoxicity against human HL60 cells assessed as cell viability after 48 hrs by MTT assay	[PMID: 21381696]
HT-1080	ED₅₀	1.04 μg/mL Compound: 2	Cytotoxicity against human HT1080 cells after 48 hrs Cytotoxicity against human HT1080 cells after 48 hrs	[PMID: 2614419]
KB	ED₅₀	1.3 μg/mL Compound: 2	Cytotoxicity against human KB cells after 72 hrs Cytotoxicity against human KB cells after 72 hrs	[PMID: 2614419]
Lu1	ED₅₀	0.84 μg/mL Compound: 2	Cytotoxicity against human Lu1 cells Cytotoxicity against human Lu1 cells	[PMID: 2614419]
P388	ED₅₀	0.66 μg/mL Compound: 2	Cytotoxicity against mouse P388 cells after 48 hrs Cytotoxicity against mouse P388 cells after 48 hrs	[PMID: 2614419]
SK-BR-3	IC₅₀	1.7 μM Compound: 28	Cytotoxicity against human SK-BR-3 cells assessed as cell viability after 48 hrs by MTT assay Cytotoxicity against human SK-BR-3 cells assessed as cell viability after 48 hrs by MTT assay	[PMID: 21381696]
SK-MEL-2	ED₅₀	2.05 μg/mL Compound: 2	Cytotoxicity against human SK-MEL-2 cells after 72 hrs Cytotoxicity against human SK-MEL-2 cells after 72 hrs	[PMID: 2614419]

In Vitro

28-Deoxonimbolide (1-100 μM, 48 h) exhibits potent cytotoxic activity against HL60 (leukemia), A549 (lung), AZ521 (stomach), SK-BR-3 (breast), CRL1579 (melanoma), and RPMI1788 (normal lymphocyte) cells^[1].
28-Deoxonimbolide (30 μM, 0-24 h) induces apoptosis in HL60 cells^[1].
28-Deoxonimbolide (30 μM, 0-24 h) increases the levels of cleaved caspases-8, -9, and -3^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.

Cell Cytotoxicity Assay^[1]

View Protocol

Cell Line:

HL60, A549, AZ521, SK-BR-3, CRL1579 cells, RPMI1788
Concentration:

1-100 μM
Incubation Time:

48 h
Result:

Exhibited potent cytotoxic activity against HL60 (leukemia), A549 (lung), AZ521 (stomach), SK-BR-3 (breast), and CRL1579 (melanoma) cells, with IC₅₀ values of 2.7 μM, 9.3 μM, 2.4 μM, 1.7 μM, 14.2 μM, and 2.7 μM, respectively.

Apoptosis Analysis^[1]

View Protocol

Cell Line:

HL60 cells
Concentration:

30 μM
Incubation Time:

8 and 24 h
Result:

Induced early apoptosis in HL60 cells. The ratio of early apoptotic cells was increased after 8 h (10.9% vs 6.1% of negative control) and 24 h (18.7% vs 3.5% of negative control), and that of late apoptotic cells (upper right) was increased after 24 h (28.1% vs 2.9% of negative control).

Western Blot Analysis^[1]

View Protocol

Cell Line:

HL60 cells
Concentration:

30 μM
Incubation Time:

8 and 24 h
Result:

Diminished the levels of procaspases-8, -9, and -3, and then increased the levels of cleaved caspases-8, -9, and -3.

Chemical Information

CAS No. 126005-94-5
Molecular Weight 452.54
Formula C₂₇H₃₂O₆
SMILES

O=C(OC)C[C@@H]([C@]1(C)C2=C(C)[C@H](C3=COC=C3)C[C@@]2([H])O[C@]1([H])[C@@](OC4)([H])[C@@]5([H])[C@@]4(C)C=C6)[C@@]5(C)C6=O
Structure Classification
- Terpenoids
- Triterpenes
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Protocols

All Cell Biology Signal Pathway Related

Detection of Apoptosis
Apoptosis, also called programmed cell death, is generally characterized by distinct morphological characteristics.
Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
Mammalian live/dead viability and cytotoxicity staining
Live/dead viability and cytotoxicity staining assays are based on the simultaneous detection of intracellular esterase activity in metabolically active (viable) cells and membrane integrity loss in non-viable cells. In commonly used dual-staining approaches, membrane-permeant fluorogenic substrates are converted by intracellular esterases into fluorescent products in live cells, while impermeant DNA-binding dyes selectively enter cells with compromised plasma membranes and label nucleic acids in dead or dying cells, enabling discrimination between viable and non-viable populations by fluorescence microscopy or flow cytometry.
Apoptosis Solutions
Apoptosis is a regulated, generally non-lytic cell-death pathway that removes unwanted, damaged, infected, or abnormal cells through coordinated morphological changes, caspase activation, DNA fragmentation, and membrane remodeling. The intrinsic apoptosis pathway is controlled mainly by mitochondrial outer membrane permeabilization, BCL-2 family proteins, cytochrome c release, apoptosome formation, caspase-9 activation, and downstream executioner caspase-3/7 activation. The extrinsic apoptosis pathway is initiated by death receptors such as Fas, TNFR, and TRAIL receptors, which recruit adaptor proteins and activate caspase-8 before engaging executioner caspases or mitochondrial amplification through BID cleavage. Apoptosis is linked to many phenotypes, including cancer cell killing, tissue homeostasis, immune regulation, neurodegeneration, infection response, and treatment-induced cytotoxicity; unresolved questions include how apoptosis interacts with necroptosis, pyroptosis, ferroptos

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (280 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Kikuchi T, et al. Cytotoxic and apoptosis-inducing activities of limonoids from the seeds of Azadirachta indica (neem). J Nat Prod. 2011 Apr 25;74(4):866-70. [Content Brief]

Calculators

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Protocols
Purity & Documentation
References

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28-Deoxonimbolide

All Caspase Isoforms

Biological Activity

Description

IC50 & Target

Cellular Effect

In Vitro

Chemical Information

Protocols

Purity & Documentation

References

Calculators

Help & FAQs