Milbemycin oxime

Name: Milbemycin oxime
Brand: MedChemExpress
SKU: HY-B0778
Rating: 4.5 (1 reviews)

Cat. No.: HY-B0778 Purity: 99.82%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Milbemycin oxime is an orally active macrolide with broad-spectrum antiparasitic activity. Milbemycin oxime is a mixture of oximes consisting of oxime derivatives corresponding to milbemycin A4 and A3. Milbemycin oxime binds to glutamate-gated chloride channels and has inhibitory potency against intestinal nematodes and lung/heart worms.

For research use only. We do not sell to patients.

Milbemycin oxime Chemical Structure

CAS No. : 129496-10-2

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent
10 mM * 1 mL in DMSO ready for reconstitution	USD 72	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 72	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 38	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 60	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 84	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 120	In-stock	Estimated Time of Arrival: December 31
200 mg	USD 180	In-stock	Estimated Time of Arrival: December 31
500 mg		Get quote
1 g		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

Macrolide

In Vivo

Milbemycin oxime (0.5 mg/kg, p.o.) and Afoxolaner (2.5 mg/kg, p.o.) prevents Angiostrongylus vasorum infection in dogs^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Angiostrongylus vasorum infected dogs^[3]
Dosage:	0.5 g, 1 g, and 2 g containing 9.375 mg + 1.875 mg, 18.75 mg + 3.75 mg, and 37.5 mg + 7.5 mg .
Administration:	p.o., on study days 0, 28, 56 and 84 with a combination of Afoxolaner plus Milbemycin oxime chewable tablets.
Result:	Reduced the geometric mean worm count and geometric mean faecal larval counts in the treated group.

Molecular Weight

1097.38

Formula

C₆₃H₈₈N₂O₁₄

CAS No.

129496-10-2

Appearance

Solid

Color

White to off-white

SMILES

CC1=C[C@@H]2[C@@]3(O)[C@H](OC/C3=C\C=C\[C@H](C)C/C(C)=C/C[C@@H](O[C@@]4(CC[C@H](C)[C@@H](C)O4)C5)C[C@@H]5OC2=O)/C1=N\O.CC6=C[C@@H]7[C@@]8(O)[C@H](OC/C8=C\C=C\[C@H](C)C/C(C)=C/C[C@@H](O[C@@]9(CC[C@H](C)[C@@H](CC)O9)C%10)C[C@@H]%10OC7=O)/C6=N\O

Structure Classification

Others

Initial Source

Microorganisms

Streptomyces hygroscopicus

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : ≥ 100 mg/mL (91.13 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	0.9113 mL	4.5563 mL	9.1126 mL
5 mM	0.1823 mL	0.9113 mL	1.8225 mL
10 mM	0.0911 mL	0.4556 mL	0.9113 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (2.28 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 2

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (1.90 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (1.90 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.82%

Select Batch:

Data Sheet (276 KB) SDS (0 KB)

COA (251 KB) LCMS (106 KB)

Handling Instructions (2659 KB)

References

[1]. Valentina M Merola, et al. Toxicology of avermectins and milbemycins (macrocylic lactones) and the role of P-glycoprotein in dogs and cats. Vet Clin North Am Small Anim Pract. 2012 Mar;42(2):313-33, vii. [Content Brief]

[2]. Dejan Cvejic, et al. Efficacy of a single dose of milbemycin oxime/praziquantel combination tablets, Milpro(®), against adult Echinococcus multilocularis in dogs and both adult and immature E. multilocularis in young cats. Parasitol Res. 2016 Mar;115(3):1195-202. [Content Brief]

[3]. Lebon W, et al. Monthly administrations of milbemycin oxime plus afoxolaner chewable tablets to prevent Angiostrongylus vasorum infection in dogs. Parasit Vectors. 2016 Sep 2;9(1):485. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	0.9113 mL	4.5563 mL	9.1126 mL	22.7815 mL
	5 mM	0.1823 mL	0.9113 mL	1.8225 mL	4.5563 mL
	10 mM	0.0911 mL	0.4556 mL	0.9113 mL	2.2782 mL
	15 mM	0.0608 mL	0.3038 mL	0.6075 mL	1.5188 mL
	20 mM	0.0456 mL	0.2278 mL	0.4556 mL	1.1391 mL
	25 mM	0.0365 mL	0.1823 mL	0.3645 mL	0.9113 mL
	30 mM	0.0304 mL	0.1519 mL	0.3038 mL	0.7594 mL
	40 mM	0.0228 mL	0.1139 mL	0.2278 mL	0.5695 mL
	50 mM	0.0182 mL	0.0911 mL	0.1823 mL	0.4556 mL
	60 mM	0.0152 mL	0.0759 mL	0.1519 mL	0.3797 mL
	80 mM	0.0114 mL	0.0570 mL	0.1139 mL	0.2848 mL