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  2. Bacterial
    Antibiotic
  3. Thiostrepton

Thiostrepton 

Cat. No.: HY-B0990 Purity: 99.80%
Handling Instructions

Thiostrepton is a thiazole antibiotic which selectively inhibits FOXM1. FOXM1 binds to YAP/TEAD complex. YAP/TEAD/FOXM1 complex binding at regulatory regions of genes governing cell cycle may impact cell proliferation.

For research use only. We do not sell to patients.

Thiostrepton Chemical Structure

Thiostrepton Chemical Structure

CAS No. : 1393-48-2

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 66 In-stock
Estimated Time of Arrival: December 31
50 mg USD 60 In-stock
Estimated Time of Arrival: December 31
100 mg   Get quote  
200 mg   Get quote  

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Customer Review

Based on 7 publication(s) in Google Scholar

Top Publications Citing Use of Products

    Thiostrepton purchased from MCE. Usage Cited in: Oncogene. 2018 Oct;37(41):5520-5533.

    Proliferation of CLL cells with lentivirus mediated FoxM1 knockdown or incubation of 1 μM FoxM1 inhibitor Thiostrepton treatment reduces significantly relative to the control group (p<0.05).

    Thiostrepton purchased from MCE. Usage Cited in: Environ Pollut. 2018 Aug 17;242(Pt B):1535-1545.

    RWPE-1 cells are pretreated with Thiostrepton at 5 µM for 1 h and subsequently exposed to MC-LR (100 nM) for 48 h; expressions of FOXM1, β-catenin, CyclinD1 and PSA are detected by western blotting. Expression levels of FOXM1 and β-catenin in the nuclei of RWPE-1 cells are examined by western blotting assay.
    • Biological Activity

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    • Customer Review

    Description

    Thiostrepton is a thiazole antibiotic which selectively inhibits FOXM1. FOXM1 binds to YAP/TEAD complex. YAP/TEAD/FOXM1 complex binding at regulatory regions of genes governing cell cycle may impact cell proliferation[1].

    In Vitro

    Thiostrepton (0.01-1000 μM; 48 hours) suppresses cell viability in A2780 and HEC-1A[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Cell Viability Assay[2]

    Cell Line: A2780 and HEC-1A cells
    Concentration: 0.01, 0.1, 1, 10, 100, 1000 μM
    Incubation Time: 48 hours
    Result: The IC50s are 1.10 μM in A2780 and 2.22 μM in HEC-1A, respectively.
    In Vivo

    Thiostrepton (i.p.; 17 mg/kg) reduces the tumorigenicity of Ewing's sarcoma (EWS) cells. Tumor volumes in control mice have increased ~6-fold from the initiation of treatment, while their Thiostrepton-treated counterparts increase only ~1.7-fold, exhibiting a ~3.5-fold reduction, relative to controls[3].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Athymic (BALB/c nu/nu) nude mice bearing A4573 cells[3]
    Dosage: 17 mg/kg
    Administration: Administered i.p.
    Result: Treatment inhibited the growth of EWS-derived tumors in vivo.
    Molecular Weight

    1664.89

    Formula

    C₇₂H₈₅N₁₉O₁₈S₅

    CAS No.
    SMILES

    O=C([[email protected]@H](NC(C(NC([[email protected]@H](NC(C(NC(C=C1)C2([H])O)[[email protected]@H](C)CC)=O)C)=O)=C)=O)C)N[[email protected]](C3=NC(C(NC(C(N/C(C4=N[[email protected]]5([H])CS4)=C\C)=O)[[email protected]](O)C)=O)=CS3)(CCC(C6=NC(C(NC(C(NC(C(N)=O)=C)=O)=C)=O)=CS6)=N7)[[email protected]@]7([H])C8=CSC([[email protected]@]([[email protected]](OC(C9=CC([[email protected]@H](O)C)=C1C2=N9)=O)C)([H])NC(C%10=CSC([[email protected]](NC5=O)([H])[[email protected]](C)(O)[[email protected]](O)C)=N%10)=O)=N8

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -80°C 2 years
    -20°C 1 year
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 100 mg/mL (60.06 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 0.6006 mL 3.0032 mL 6.0064 mL
    5 mM 0.1201 mL 0.6006 mL 1.2013 mL
    10 mM 0.0601 mL 0.3003 mL 0.6006 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: 2.5 mg/mL (1.50 mM); Suspended solution; Need ultrasonic

    • 2.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2.5 mg/mL (1.50 mM); Clear solution

    *All of the co-solvents are provided by MCE.
    References

    Purity: 99.80%

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    Keywords:

    ThiostreptonBacterialAntibioticInhibitorinhibitorinhibit

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