Thiostrepton
Based on 24 publication(s) in Google Scholar
Thiostrepton is a thiazole antibiotic which selectively inhibits FOXM1. FOXM1 binds to YAP/TEAD complex. YAP/TEAD/FOXM1 complex binding at regulatory regions of genes governing cell cycle may impact cell proliferation.
For research use only. We do not sell to patients.
- Purity: 99.80%
- CAS No.: 1393-48-2
- Formula: C72H85N19O18S5
- Molecular Weight:1664.89
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Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Thiostrepton
More- Cell Death Dis. 2022 Jul 20;13(7):630. [Abstract]
- Phytomedicine. 2024 Jul:129:155656. [Abstract]
- Chemosphere. 2021 Jan;263:128295. [Abstract]
- J Transl Med. 2024 Sep 27;22(1):871. [Abstract]
- Stem Cell Res Ther. 2024 Sep 18;15(1):309. [Abstract]
- Oncogene. 2018 Oct;37(41):5520-5533. [Abstract]
- Environ Pollut. 2018 Aug 17;242(Pt B):1535-1545. [Abstract]
- J Autoimmun. 2025 Jun 2:154:103442. [Abstract]
- Anal Chem. 2023 Apr 11;95(14):6191. [Abstract]
- Ecotoxicol Environ Saf. 2025 Apr 15:296:118193. [Abstract]
- Ecotoxicol Environ Saf. 2021 Apr 1:212:111931. [Abstract]
- Biochem Pharmacol. 2026 May:247:117761. [Abstract]
- Life Sci. 2025 May 7:123691. [Abstract]
- Int J Mol Sci. 2024 Sep 8;25(17):9717. [Abstract]
- Mol Oncol. 2019 Feb;13(2):228-245. [Abstract]
- Transl Oncol. 2025 Feb 21:54:102327. [Abstract]
- iScience. 2025 Jan 21;28(2):111856. [Abstract]
- Cell Signal. 2024 Jul 3:111285. [Abstract]
- Exp Cell Res. 2023 Mar 15;424(2):113505. [Abstract]
- Acta Biochim Biophys Sin (Shanghai). 2024 Apr 25;56(4):621-633. [Abstract]
- Gut Liver. 2024 May 15;18(3):476-488. [Abstract]
- Gene. 2020 Oct 5;757:144947. [Abstract]
- J Asian Nat Prod Res. 2024 Aug;26(8):945-954. [Abstract]
- Research Square Preprint. 2022 Jan.
Customer Validation & Images
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Cell Proliferation/Viability Assay
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WB
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Cell Imaging/Staining
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IF
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IHC
All Antibiotic Isoforms
MoreAll YAP Isoforms
More
Biological Activity
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YAP/TAZ-TEAD |
Thiostrepton (0.01-1000 μM; 48 hours) suppresses cell viability in A2780 and HEC-1A[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:A2780 and HEC-1A cells
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Concentration:0.01, 0.1, 1, 10, 100, 1000 μM
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Incubation Time:48 hours
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Result:The IC50s are 1.10 μM in A2780 and 2.22 μM in HEC-1A, respectively.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Athymic (BALB/c nu/nu) nude mice bearing A4573 cells[3]
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Dosage:17 mg/kg
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Administration:Administered i.p.
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Result:Treatment inhibited the growth of EWS-derived tumors in vivo.
Chemical Information
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CAS No. 1393-48-2
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Appearance Solid
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Molecular Weight 1664.89
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Formula C72H85N19O18S5
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Color White to off-white
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Structure Classification
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Initial Source
bacteria of Streptomyces genus
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (24)
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Journal Impact Factor
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Most Recent
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Cell Death Dis
Thiostrepton induces ferroptosis in pancreatic cancer cells through STAT3/GPX4 signalling. [Abstract]2022 Jul 20;13(7):630. PMID: 35859150
Thiostrepton purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2022 Jul 20;13(7):630. [Abstract]
Thiostrepton (TST) (2-5 μM) significantly inhibited the clonogenesis of Panc-1, MIAPaCa-2 and BxPC-3 cells.
Thiostrepton purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2022 Jul 20;13(7):630. [Abstract]
Thiostrepton (TST) (2-5 μM; 48 h) significantly reduced factor forkhead box M1 (FOXM1) protein expression levels in Panc-1, MIAPaCa-2 and BxPC-3 cells.
Thiostrepton purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2022 Jul 20;13(7):630. [Abstract]
Thiostrepton (TST) (2 μM; 24 h) significantly increased ROS production in Panc-1, MIA PaCa-2, and BxPC-3 cells.
Thiostrepton purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2022 Jul 20;13(7):630. [Abstract]
Thiostrepton (TST) (2-5 μM) reduced the expression of STAT3 both in nucleus and cytoplasm of Panc-1, MIA PaCa-2, and BxPC-3 cells.
Thiostrepton purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2022 Jul 20;13(7):630. [Abstract]
Thiostrepton (TST) (17 mg/kg; i.p.; three times a week) effectively decreased the levels of FOXM1, STAT3, p-STA3, GPX4, and Ki67 proteins in the subcutaneous xenograft model mice.
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Phytomedicine
Huaier enhances the tumor-killing effect and reverses gemcitabine-induced stemness by suppressing FoxM1. [Abstract]2024 Jul:129:155656. PMID: 38723529 -
Chemosphere
Chronic exposure to microcystin-LR increases the risk of prostate cancer and induces malignant transformation of human prostate epithelial cells. [Abstract]2021 Jan;263:128295. PMID: 33297237 -
J Transl Med
Vascular restenosis following paclitaxel-coated balloon therapy is attributable to NLRP3 activation and LIN9 upregulation. [Abstract]2024 Sep 27;22(1):871. PMID: 39334121 -
Stem Cell Res Ther
Senescent lung-resident mesenchymal stem cells drive pulmonary fibrogenesis through FGF-4/FOXM1 axis. [Abstract]2024 Sep 18;15(1):309. PMID: 39289765 -
Oncogene
Inhibition of maternal embryonic leucine zipper kinase with OTSSP167 displays potent anti-leukemic effects in chronic lymphocytic leukemia. [Abstract]2018 Oct;37(41):5520-5533. PMID: 29895969
Thiostrepton purchased from MedChemExpress. Usage Cited in: Oncogene. 2018 Oct;37(41):5520-5533. [Abstract]
Proliferation of CLL cells with lentivirus mediated FoxM1 knockdown or incubation of 1 μM FoxM1 inhibitor Thiostrepton treatment reduces significantly relative to the control group (p<0.05).
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Environ Pollut
Chronic exposure to microcystin-leucine-arginine promoted proliferation of prostate epithelial cells resulting in benign prostatic hyperplasia. [Abstract]2018 Aug 17;242(Pt B):1535-1545. PMID: 30145517
Thiostrepton purchased from MedChemExpress. Usage Cited in: Environ Pollut. 2018 Aug 17;242(Pt B):1535-1545. [Abstract]
RWPE-1 cells are pretreated with Thiostrepton at 5 µM for 1 h and subsequently exposed to MC-LR (100 nM) for 48 h; expressions of FOXM1, β-catenin, CyclinD1 and PSA are detected by western blotting. Expression levels of FOXM1 and β-catenin in the nuclei of RWPE-1 cells are examined by western blotting assay.
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J Autoimmun
The phenotypic and functional characteristics of intrahepatic CD69+CD103+ tissue-resident MAIT cells in primary biliary cholangitis. [Abstract]2025 Jun 2:154:103442. PMID: 40460780 -
Anal Chem
Label-Free Isolation of Low-Adhesion Cells with Stem Properties for Cancer Stem Cell-Specific Drug Evaluation. [Abstract]2023 Apr 11;95(14):6191. PMID: 36122350 -
Ecotoxicol Environ Saf
Adolescent cetylpyridinium chloride exposure impairs homologous recombination repair and induces granulosa cell apoptosis and follicular atresia via FOXM1/CREBBP complex suppression. [Abstract]2025 Apr 15:296:118193. PMID: 40239548 -
Ecotoxicol Environ Saf
The mechanisms of mitochondrial dysfunction and glucose intake decrease induced by Microcystin-LR in ovarian granulosa cells. [Abstract]2021 Apr 1:212:111931. PMID: 33508714 -
Biochem Pharmacol
Momordin Ic suppresses breast cancer growth by targeting ACTL8‑dependent glutamine metabolism and PI3K/AKT/mTOR-MYC. [Abstract]2026 May:247:117761. PMID: 41621692 -
Life Sci
FoxM1 promotes the proliferation of cervical adenocarcinoma cells through transcriptional activation of FAM83D. [Abstract]2025 May 7:123691. PMID: 40345484 -
Int J Mol Sci
2024 Sep 8;25(17):9717. PMID: 39273665 -
Mol Oncol
Hypoxia-driven paracrine osteopontin/integrin αvβ3 signaling promotes pancreatic cancer cell epithelial-mesenchymal transition and cancer stem cell-like properties by modulating forkhead box protein M1. [Abstract]2019 Feb;13(2):228-245. PMID: 30367545 -
Transl Oncol
Thiostrepton suppresses intrahepatic cholangiocarcinoma progression via FOXM1-mediated tumor-associated macrophages reprogramming. [Abstract]2025 Feb 21:54:102327. PMID: 39986191 -
iScience
The matricellular protein Fibulin-5 regulates β-cell proliferation in an autocrine/paracrine manner. [Abstract]2025 Jan 21;28(2):111856. PMID: 39995864 -
Cell Signal
Thiostrepton induces oxidative stress, mitochondrial dysfunction and ferroptosis in HaCaT cells. [Abstract]2024 Jul 3:111285. PMID: 38969192 -
Exp Cell Res
Inhibition of YTHDF1 prevents hypoxia-induced pulmonary artery smooth muscle cell proliferation by regulating Foxm1 translation in an m6A-dependent manner. [Abstract]2023 Mar 15;424(2):113505. PMID: 36736607 -
Acta Biochim Biophys Sin (Shanghai)
Lian-Mei-Yin formula alleviates diet-induced hepatic steatosis by suppressing Yap1/FOXM1 pathway-dependent lipid synthesis. [Abstract]2024 Apr 25;56(4):621-633. PMID: 38516704 -
Gut Liver
Activin and Hepatocyte Growth Factor Promotes Colorectal Cancer Stemness and Metastasis through FOXM1/SOX2/CXCR4 Signaling. [Abstract]2024 May 15;18(3):476-488. PMID: 37458065 -
Gene
2020 Oct 5;757:144947. PMID: 32659254 -
J Asian Nat Prod Res
Thiostrepton suppresses triple-negative breast cancer through downregulating c-FLIP/SMAD2/3 signaling pathway. [Abstract]2024 Aug;26(8):945-954. PMID: 38634704 -
Solvent & Solubility
DMSO : 125 mg/mL (75.08 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 5 mg/mL (3.00 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 5 mg/mL (3.00 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (288 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[1]. Ajaybabu V Pobbati, et al. A combat with the YAP/TAZ-TEAD oncoproteins for cancer therapy. Theranostics. 2020 Feb 18;10(8):3622-3635. [Content Brief]
[2]. Xuan Zhang, et al. Targeting of mutant p53-induced FoxM1 with Thiostrepton induces cytotoxicity and enhances carboplatin sensitivity in cancer cells. Oncotarget. 2014 Nov 30;5(22):11365-80. [Content Brief]
[3]. Aniruddha Sengupta, et al. The dual inhibitory effect of Thiostrepton on FoxM1 and EWS/FLI1 provides a novel therapeutic option for Ewing's sarcoma. Int J Oncol. 2013 Sep;43(3):803-12. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 0.6006 mL | 3.0032 mL | 6.0064 mL | 15.0160 mL |
| 5 mM | 0.1201 mL | 0.6006 mL | 1.2013 mL | 3.0032 mL | |
| 10 mM | 0.0601 mL | 0.3003 mL | 0.6006 mL | 1.5016 mL | |
| 15 mM | 0.0400 mL | 0.2002 mL | 0.4004 mL | 1.0011 mL | |
| 20 mM | 0.0300 mL | 0.1502 mL | 0.3003 mL | 0.7508 mL | |
| 25 mM | 0.0240 mL | 0.1201 mL | 0.2403 mL | 0.6006 mL | |
| 30 mM | 0.0200 mL | 0.1001 mL | 0.2002 mL | 0.5005 mL | |
| 40 mM | 0.0150 mL | 0.0751 mL | 0.1502 mL | 0.3754 mL | |
| 50 mM | 0.0120 mL | 0.0601 mL | 0.1201 mL | 0.3003 mL | |
| 60 mM | 0.0100 mL | 0.0501 mL | 0.1001 mL | 0.2503 mL |
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