ARP-100

Name: ARP-100
Brand: MedChemExpress
SKU: HY-103444
Rating: 4.5 (1 reviews)

Cat. No.: HY-103444 Purity: 98.07%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

ARP-100 is a potent and selective matrix metalloproteinase MMP-2 inhibitor (IC₅₀=12 nM). ARP-100 interacts with S1＇ pocket of MMP-2 and shows anti-invasive properties in an in vitro model of invasion on matrigel. ARP-100 shows the less inhibitory activity towards MMP-1 (>50 μM), MMP-3 (4.5 μM), MMP-7 (>50 μM), and MMP-9 (0.2 μM).

For research use only. We do not sell to patients.

CAS No. : 704888-90-4

Size	Stock	Quantity

Free Sample (0.1 - 0.2 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

or Bulk Inquiry

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of ARP-100:

ARP-100 (Standard) Get quote

1 Publications Citing Use of MCE ARP-100

•Invest Ophthalmol Vis Sci. 2025 May 1;66(5):19. [Abstract]

RT-PCR

In Vivo Efficacy Study

: ARP-100 purchased from MedChemExpress. Usage Cited in: Invest Ophthalmol Vis Sci. 2025 May 1;66(5):19. [Abstract]; The fluorescence intensity of MMP-2 in the HFSFs treated with glutathione oxidized (GLU, 2.48 mg/mL, 24 h) was 2.09 times greater than that in the HFSFs treated with ARP-100 (ARP, 100 μM, 24 h).

: ARP-100 purchased from MedChemExpress. Usage Cited in: Invest Ophthalmol Vis Sci. 2025 May 1;66(5):19. [Abstract]; The MMP-2 mRNA level in the HFSFs treated with glutathione oxidized (GLU, 2.48 mg/mL, 24 h) was 2.46 times greater than that in the ARP-100 (ARP, 100 μM, 24 h)-treated HFSFs.

: ARP-100 purchased from MedChemExpress. Usage Cited in: Invest Ophthalmol Vis Sci. 2025 May 1;66(5):19. [Abstract]; IF analysis revealed a notable increase in collagen I fluorescence intensity in the HFSFs treated with ARP-100 (ARP, 100 μM, 24 h), whereas a reduction in collagen I fluorescence intensity was found in HFSFs treated with glutathione oxidized (GLU, 2.48 mg/mL, 24 h).

: ARP-100 purchased from MedChemExpress. Usage Cited in: Invest Ophthalmol Vis Sci. 2025 May 1;66(5):19. [Abstract]; WB analysis of MMP-2 and collagen I expression in HFSFs following treatment with ARP-100 (ARP, 100 μM), glutathione oxidized (GLU, 2.48 mg/mL), or PBS for 24 hours.

: ARP-100 purchased from MedChemExpress. Usage Cited in: Invest Ophthalmol Vis Sci. 2025 May 1;66(5):19. [Abstract]; Compared with guinea pigs receiving periocular injections of vehicle (5 mM, 10 μL on days 1, 4, 7, 10, and 13 over two weeks), those treated with glutathione oxidized (Glu, 20 mg/mL, 10 μL on the same schedule) exhibited a 1.76-fold increase in relative myopia, along with a 1.78-fold increase in axial length (AL).

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View All MMP Isoform Specific Products:

View All Isoforms

MMP-1 MMP-2 MMP-3 MMP-8 MMP-9 MMP-13 MMP-14 MMP-17 ADAM10 ADAM17 MMP-7 MMP-12 MMP-10 MMP ADAM8 MMP-19

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[1]

MMP-2

12 nM (IC₅₀)

MMP-9

0.2 μM (IC₅₀)

MMP-3

4.5 μM (IC₅₀)

MMP-1

>50 μM (IC₅₀)

MMP-7

>50 μM (IC₅₀)

In Vitro

ARP-100 (50 nM) shows a significant reduction in the total number of invasive elongations^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

364.42

Formula

C₁₇H₂₀N₂O₅S

CAS No.

704888-90-4

Appearance

Solid

Color

Off-white to light yellow

SMILES

O=C(NO)CN(S(=O)(C1=CC=C(C2=CC=CC=C2)C=C1)=O)OC(C)C

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (274.41 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.7441 mL	13.7204 mL	27.4409 mL
5 mM	0.5488 mL	2.7441 mL	5.4882 mL
10 mM	0.2744 mL	1.3720 mL	2.7441 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.86 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (6.86 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (6.86 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: ≥99.0%

Select Batch:

Data Sheet (276 KB) SDS (604 KB)

COA (251 KB)

Handling Instructions (2659 KB)

References

[1]. Rossello A, et al. New N-arylsulfonyl-N-alkoxyaminoacetohydroxamic acids as selective inhibitors of gelatinase A (MMP-2). Bioorg Med Chem. 2004 May 1;12(9):2441-50. [Content Brief]

[2]. Tuccinardi T, et al. Amber force field implementation, molecular modelling study, synthesis and MMP-1/MMP-2 inhibition profile of (R)- and (S)-N-hydroxy-2-(N-isopropoxybiphenyl-4-ylsulfonamido)-3-methylbutanamides. Bioorg Med Chem. 2006 Jun 15;14(12):4260-76. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.7441 mL	13.7204 mL	27.4409 mL	68.6022 mL
	5 mM	0.5488 mL	2.7441 mL	5.4882 mL	13.7204 mL
	10 mM	0.2744 mL	1.3720 mL	2.7441 mL	6.8602 mL
	15 mM	0.1829 mL	0.9147 mL	1.8294 mL	4.5735 mL
	20 mM	0.1372 mL	0.6860 mL	1.3720 mL	3.4301 mL
	25 mM	0.1098 mL	0.5488 mL	1.0976 mL	2.7441 mL
	30 mM	0.0915 mL	0.4573 mL	0.9147 mL	2.2867 mL
	40 mM	0.0686 mL	0.3430 mL	0.6860 mL	1.7151 mL
	50 mM	0.0549 mL	0.2744 mL	0.5488 mL	1.3720 mL
	60 mM	0.0457 mL	0.2287 mL	0.4573 mL	1.1434 mL
	80 mM	0.0343 mL	0.1715 mL	0.3430 mL	0.8575 mL
	100 mM	0.0274 mL	0.1372 mL	0.2744 mL	0.6860 mL

ARP-100 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

ARP-100704888-90-4ARP100ARP 100MMPMatrix metalloproteinasesmatrixmetalloproteinaseselectiveanti-invasivematrigelinvasionInhibitorinhibitorinhibit

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ARP-100

Customer Review

Other Forms of ARP-100:

ARP-100 Related Antibodies

1 Publications Citing Use of MCE ARP-100

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All MMP Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

ARP-100 Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

ARP-100 Related Classifications

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Your Recently Viewed Products:

Customer Review

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