Art. -Nr.: HY-N4323

14-Deoxyandrographolide

1 Cited Publications

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14-Deoxyandrographolide is a diterpene with calcium channel blocking activity and acts as a uterine smooth muscle relaxant. 14-Deoxyandrographolide stimulates the release of nitric oxide (NO) in endothelial cells. 14-Deoxyandrographolide gradually desensitizes liver cells to TNF-α mediated apoptosis by inducing the release of TNFRSF1A.

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Reinheit: 99.94%
CAS. Nr.: 4176-97-0
Formel: C₂₀H₃₀O₄
Molecular Weight:334.45

Speicherung:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

14-Deoxyandrographolide Chemical Structure

CAS. Nr.: 4176-97-0

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Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (1)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target

Caspase 3

Alle Calcium Channel Isoform-spezifische Produkte anzeigen :

Alle Caspase Isoform-spezifische Produkte anzeigen :

Cellular Effect

Cell Line	Type	Value	Description	References
HL-60	GI₅₀	25.46 μM Compound: 9	Antiproliferative activity against human HL60 cells by trypan blue assay Antiproliferative activity against human HL60 cells by trypan blue assay	[PMID: 18357994]
NIH3T3	IC₅₀	176.3 μM Compound: 5	Antifibrotic activity against mouse NIH/3T3 cells assessed as inhibition of cell proliferation after 72 hrs by MTT assay Antifibrotic activity against mouse NIH/3T3 cells assessed as inhibition of cell proliferation after 72 hrs by MTT assay	[PMID: 30144698]
NTUB1	IC₅₀	>100 μM Compound: 4	Cytotoxicity against human NTUB1 cells after 48 hrs by MTT assay Cytotoxicity against human NTUB1 cells after 48 hrs by MTT assay	[PMID: 25913115]
RAW264.7	IC₅₀	21.01 μM Compound: 1	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production after 24 hrs by Griess method Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production after 24 hrs by Griess method	[PMID: 22264489]

In Vitro

14-Deoxyandrographolide can reduce uterine smooth muscle contraction responses induced by 0.3 mM and 3.0 mM CaCl², with IC₅₀ values of 1.24 M and 5.94 M^[1].
14-Deoxyandrographolide (0-15 nM, 1 h) shows no cytotoxicity in primary liver cells and prevents TNF-α induced cell death^[2].
14-Deoxyandrographolide (5-15 nM, 1 h) inhibits the expression of caspase-3 in primary liver cells^[2].
14-Deoxyandrographolide (10 nM, 1 h) suppressed the aggregation of TRADD, FADD and caspase-8 in TNF-a sensitized liver cells and inhibited the formation of the DISC^[2].
14-Deoxyandrographolide (10 nM, 1 h) induces the release of TNFRSF1A from liver cells^[2].
14-Deoxyandrographolide (0-100 μM, 24-48 h) stimulates endothelial cells to produce NO in a concentration-dependent manner^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:

Primary hepatocytes
Concentration:

0, 2.5, 5, 7.5, 10, 12.5, 15 nM
Incubation Time:

1 h
Result:

Reduced cell death rate.

Western Blot Analysis^[2]

Cell Line:

Primary hepatocytes
Concentration:

5, 10, 15 nM
Incubation Time:

1 h
Result:

Inhibited the expression of caspase-3 in a concentration-dependent manner.

In Vivo

14-Deoxyandrographolide (40 mg/kg, intraperitoneal injection, single dose) makes rat liver cells resistant to apoptosis induced by TNF-α by downregulating the formation of death-inducing signaling complex. It enhances the activity of microsomal Ca-ATPase and increases calcium ion influx into the microsomal lumen through the NO/cGMP pathway, which in turn promotes the release of TNFRSF1A^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Male Sprague-Dawley rats^[2]
Dosage:

40 mg/kg; single dose
Administration:

Intraperitoneal injection (i.p.)
Result:

Reduced ALT elevation caused by TNF-α/ActD, reduced the number of hepatocyte apoptosis, and reduced the amount of TNF-α retained in the liver.

Chemical Information

CAS. Nr. 4176-97-0
Appearance Solid
Molecular Weight 334.45
Formel C₂₀H₃₀O₄
Color White to off-white
SMILES

O=C1OCC=C1CC[C@@H]2C(CC[C@]3([H])[C@](C)(CO)[C@H](O)CC[C@@]23C)=C
Structure Classification
- Terpenoids
- Diterpenoids
Initial Source
- Plants
- Acanthaceae
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (1)

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Journal Impact Factor

Eur J Pharmacol

Andrographolide analogues are agonists of the estrogen receptor alpha in vitro and in vivo. [Abstract]

2026 Feb 28:1016:178641. PMID: 41643829

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (299.00 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.9900 mL

14.9499 mL

29.8998 mL

5 mM

0.5980 mL

2.9900 mL

5.9800 mL

10 mM

0.2990 mL

1.4950 mL

2.9900 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (7.47 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (7.47 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (280 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Burgos RA, et al. Effect of 14-deoxyandrographolide on calcium-mediated rat uterine smooth muscle contractility. Phytother Res. 2003;17(9):1011-1015. [Content Brief]

[2]. Roy DN, et al. 14-Deoxyandrographolide desensitizes hepatocytes to tumour necrosis factor-alpha-induced apoptosis through calcium-dependent tumour necrosis factor receptor superfamily member 1A release via the NO/cGMP pathway. Br J Pharmacol. 2010 Aug;160(7):1823-43. [Content Brief]

[3]. C Y Zhang, et al. Effects of 14-deoxyandrographolide and 14-deoxy-11,12-didehydroandrographolide on nitric oxide production in cultured human endothelial cells. Phytother Res. 1999 Mar;13(2):157-9. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.9900 mL	14.9499 mL	29.8998 mL	74.7496 mL
	5 mM	0.5980 mL	2.9900 mL	5.9800 mL	14.9499 mL
	10 mM	0.2990 mL	1.4950 mL	2.9900 mL	7.4750 mL
	15 mM	0.1993 mL	0.9967 mL	1.9933 mL	4.9833 mL
	20 mM	0.1495 mL	0.7475 mL	1.4950 mL	3.7375 mL
	25 mM	0.1196 mL	0.5980 mL	1.1960 mL	2.9900 mL
	30 mM	0.0997 mL	0.4983 mL	0.9967 mL	2.4917 mL
	40 mM	0.0747 mL	0.3737 mL	0.7475 mL	1.8687 mL
	50 mM	0.0598 mL	0.2990 mL	0.5980 mL	1.4950 mL
	60 mM	0.0498 mL	0.2492 mL	0.4983 mL	1.2458 mL
	80 mM	0.0374 mL	0.1869 mL	0.3737 mL	0.9344 mL
	100 mM	0.0299 mL	0.1495 mL	0.2990 mL	0.7475 mL

Keywords

14-Deoxyandrographolide

1 Cited Publications

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14-Deoxyandrographolide

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Publications Citing Use of MedChemExpress (MCE) 14-Deoxyandrographolide

Alle Calcium Channel Isoform-spezifische Produkte anzeigen

Alle Caspase Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (1)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

14-Deoxyandrographolide Related Classifications

HY-N4323 Related Classifications