Art. -Nr.: HY-B1473A

Serotonin

(Synonyms: 5-Hydroxytryptamine; 5-HT)

41 Cited Publications

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Based on 41 publication(s) in Google Scholar

Serotonin is a monoamine neurotransmitter in the CNS and an endogenous 5-HT receptor agonist. Serotonin is also a catechol O-methyltransferase (COMT) inhibitor with a K_i of 44 μM.

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Reinheit: 98.96%
CAS. Nr.: 50-67-9
Formel: C₁₀H₁₂N₂O
Molecular Weight:176.22

Speicherung:

-20°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Serotonin Chemical Structure

CAS. Nr.: 50-67-9

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Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (41)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Protokoll
Reinheit & Dokumentation
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Bioz

Biologische Aktivität

Beschreibung

Serotonin is a monoamine neurotransmitter in the CNS and an endogenous 5-HT receptor agonist. Serotonin is also a catechol O-methyltransferase (COMT) inhibitor with a K_i of 44 μM.

IC₅₀ & Target

Microbial Metabolite

Human Endogenous Metabolite

Alle 5-HT Receptor Isoform-spezifische Produkte anzeigen :

Alle Endogenous Metabolite Isoform-spezifische Produkte anzeigen :

Cellular Effect

Cell Line	Type	Value	Description	References
B16	IC₅₀	>30 μM Compound: 17	Antimelanogenic activity in mouse B16 cells assessed as inhibition of intracellular melanin accumulation after 2 days Antimelanogenic activity in mouse B16 cells assessed as inhibition of intracellular melanin accumulation after 2 days	[PMID: 19524439]
B16	IC₅₀	550 μM Compound: 17	Inhibition of catecholase activity of tyrosinase in mouse B16 cells assessed as dopachrome formation Inhibition of catecholase activity of tyrosinase in mouse B16 cells assessed as dopachrome formation	[PMID: 19524439]
C6	EC₅₀	12 nM Compound: 5-HT	Agonist activity at human 5HT4 isoform E receptor expressed in rat C6 cells assessed as cAMP accumulation by radio-immunoassay relative to 5HT Agonist activity at human 5HT4 isoform E receptor expressed in rat C6 cells assessed as cAMP accumulation by radio-immunoassay relative to 5HT	[PMID: 19334715]
CHO	IC₅₀	2.55 x 10^-3 μM Compound: 5-HT	Displacement of [3H]LSD from human 5HT7 receptor expressed in CHO cells Displacement of [3H]LSD from human 5HT7 receptor expressed in CHO cells	[PMID: 12713409]
CHO	EC₅₀	3.7 nM Compound: 5-HT	Tested for functional response on CHO cells expressing cloned human 5-hydroxytryptamine 1A receptor Tested for functional response on CHO cells expressing cloned human 5-hydroxytryptamine 1A receptor	[PMID: 12954071]
CHO	EC₅₀	1.5 nM Compound: 5-HT	Functional activity against human 5-hydroxytryptamine 2B receptor expressed in CHO cells using fluorometric imaging plate reader Functional activity against human 5-hydroxytryptamine 2B receptor expressed in CHO cells using fluorometric imaging plate reader	[PMID: 15081042]
CHO	EC₅₀	1.7 nM Compound: 5-HT	Functional activity against human 5-hydroxytryptamine 2C receptor expressed in CHO cells using fluorometric imaging plate reader Functional activity against human 5-hydroxytryptamine 2C receptor expressed in CHO cells using fluorometric imaging plate reader	[PMID: 15081042]
CHO	EC₅₀	11 nM Compound: 5-HT	Functional activity against human 5-hydroxytryptamine 2A receptor expressed in CHO cells using fluorometric imaging plate reader Functional activity against human 5-hydroxytryptamine 2A receptor expressed in CHO cells using fluorometric imaging plate reader	[PMID: 15081042]
CHO	EC₅₀	1.5 nM Compound: 2	Binding to human 5HT2B receptor expressed in CHO cells Binding to human 5HT2B receptor expressed in CHO cells	[PMID: 16257207]
CHO	EC₅₀	1.7 nM Compound: 2	Binding to human 5HT2C receptor expressed in CHO cells Binding to human 5HT2C receptor expressed in CHO cells	[PMID: 16257207]
CHO	EC₅₀	11 nM Compound: 2	Binding to human 5HT2A receptor expressed in CHO cells Binding to human 5HT2A receptor expressed in CHO cells	[PMID: 16257207]
CHO	EC₅₀	1 nM Compound: 5-HT	Agonistic activity at human 5HT1A in CHO cells by the inhibition of forskolin-stimulated cAMP accumulation Agonistic activity at human 5HT1A in CHO cells by the inhibition of forskolin-stimulated cAMP accumulation	[PMID: 16392798]
CHO	EC₅₀	0.7 nM Compound: 5-HT	Agonist activity assessed by stimulation of [35S]GTP-gamma-S binding to human 5HT1A receptor expressed in CHO cells Agonist activity assessed by stimulation of [35S]GTP-gamma-S binding to human 5HT1A receptor expressed in CHO cells	[PMID: 16458504]
CHO	EC₅₀	24 nM Compound: 5-HT	Agonist activity at human cloned 5HT2C receptor expressed in CHO cells assessed as myo-[3H]inositol hydrolysis Agonist activity at human cloned 5HT2C receptor expressed in CHO cells assessed as myo-[3H]inositol hydrolysis	[PMID: 18035544]
CHO	EC₅₀	70 nM Compound: 5-HT	Agonist activity at human cloned 5HT2A receptor expressed in CHO cells assessed as myo-[3H]inositol hydrolysis Agonist activity at human cloned 5HT2A receptor expressed in CHO cells assessed as myo-[3H]inositol hydrolysis	[PMID: 18035544]
CHO	EC₅₀	150 nM Compound: 5-HT	Agonist activity at 5HT1A receptor expressed in CHO cells by [35S]GTPgammaS binding assay Agonist activity at 5HT1A receptor expressed in CHO cells by [35S]GTPgammaS binding assay	[PMID: 19559623]
CHO	EC₅₀	25 nM Compound: 5-HT	Agonist activity at human recombinant 5HT2C receptor expressed in CHO K1 cells assessed as induction of calcium mobilization by FLIPR assay Agonist activity at human recombinant 5HT2C receptor expressed in CHO K1 cells assessed as induction of calcium mobilization by FLIPR assay	[PMID: 19646865]
CHO	EC₅₀	147 nM Compound: 5-HT	Agonist activity at 5HT1A receptor expressed in CHO cells assessed as stimulation of [35S]GTPgamma binding Agonist activity at 5HT1A receptor expressed in CHO cells assessed as stimulation of [35S]GTPgamma binding	[PMID: 20041669]
CHO	EC₅₀	1.63 nM Compound: 5-HT	Agonist activity at 5HT2C receptor expressed in CHO cells by fluorescence based calcium mobilization assay Agonist activity at 5HT2C receptor expressed in CHO cells by fluorescence based calcium mobilization assay	[PMID: 22381048]
CHO	IC₅₀	1.2 x 10^-7 M Compound: Serotonin	Displacement of [3H]LSD from human recombinant 5-HT6 receptor expressed in CHO cells Displacement of [3H]LSD from human recombinant 5-HT6 receptor expressed in CHO cells	[PMID: 26988801]
CHO	IC₅₀	2.5 x 10^-10 M Compound: Serotonin	Displacement of [3H]LSD from human recombinant 5-HT7 receptor expressed in CHO cells Displacement of [3H]LSD from human recombinant 5-HT7 receptor expressed in CHO cells	[PMID: 26988801]
CHO	IC₅₀	1.2 x 10^-7 M Compound: Serotonin	Displacement of [3H]LSD from human recombinant 5HT6 receptor expressed in CHO cells measured after 120 mins by scintillation counting method Displacement of [3H]LSD from human recombinant 5HT6 receptor expressed in CHO cells measured after 120 mins by scintillation counting method	[PMID: 27876250]
CHO	IC₅₀	1.2 x 10^-1 μM Compound: Serotonin	Displacement of [3H]LSD from human recombinant 5HT6 receptor expressed in CHO cells measured after 120 mins by scintillation counting method Displacement of [3H]LSD from human recombinant 5HT6 receptor expressed in CHO cells measured after 120 mins by scintillation counting method	[PMID: 27876250]
CHO	IC₅₀	2.5 x 10^-10 M Compound: Serotonin	Displacement of [3H]LSD from human recombinant 5HT7 receptor expressed in CHO cells measured after 120 mins by scintillation counting method Displacement of [3H]LSD from human recombinant 5HT7 receptor expressed in CHO cells measured after 120 mins by scintillation counting method	[PMID: 27876250]
CHO	EC₅₀	4.67 nM Compound: 1	The intrinsic activity was evaluated for its ability to inhibit forskolin-stimulated c-AMP formation mediated by cloned 5-hydroxytryptamine 1B receptor in CHO cell line; Full Agonist The intrinsic activity was evaluated for its ability to inhibit forskolin-stimulated c-AMP formation mediated by cloned 5-hydroxytryptamine 1B receptor in CHO cell line; Full Agonist	[PMID: 9871775]
CHO-K1	EC₅₀	15 nM Compound: Serotonin	Agonist activity at human recombinant 5HT7 receptor expressed in CHOK1 cells assessed as increase in cAMP levels by HTRF assay Agonist activity at human recombinant 5HT7 receptor expressed in CHOK1 cells assessed as increase in cAMP levels by HTRF assay	[PMID: 19326916]
CHO-K1	EC₅₀	16 nM Compound: 5-HT	Agonist activity at 5HT2A receptor (unknown origin) expressed in CHOK1 cells coexpressing Galpha15 by FLIPR assay Agonist activity at 5HT2A receptor (unknown origin) expressed in CHOK1 cells coexpressing Galpha15 by FLIPR assay	[PMID: 23675993]
CHO-K1	EC₅₀	39 nM Compound: 5-HT	Agonist activity at 5HT1A receptor (unknown origin) expressed in CHOK1 cells coexpressing Galpha15 by FLIPR assay Agonist activity at 5HT1A receptor (unknown origin) expressed in CHOK1 cells coexpressing Galpha15 by FLIPR assay	[PMID: 23675993]
CHO-K1	EC₅₀	15.33 nM Compound: 5-HT	Agonist activity at 5-HT1A receptor (unknown origin) expressed in CHOK1 cells co-expressing G-alpha 15 by Fluo-4 dye based FLIPR assay Agonist activity at 5-HT1A receptor (unknown origin) expressed in CHOK1 cells co-expressing G-alpha 15 by Fluo-4 dye based FLIPR assay	[PMID: 27487565]
CHO-K1	EC₅₀	47 nM Compound: 5-HT	Agonist activity at 5-HT2A receptor (unknown origin) expressed in CHOK1 cells co-expressing G-alpha 15 by Fluo-4 dye based FLIPR assay Agonist activity at 5-HT2A receptor (unknown origin) expressed in CHOK1 cells co-expressing G-alpha 15 by Fluo-4 dye based FLIPR assay	[PMID: 27487565]
CHO-K1	EC₅₀	18 nM Compound: Serotonin	Agonist activity at human recombinant 5HT1A receptor (unknown origin) stably expressed in CHO-K1 incubated for 30 mins by [35S]GTPgammaS binding assay Agonist activity at human recombinant 5HT1A receptor (unknown origin) stably expressed in CHO-K1 incubated for 30 mins by [35S]GTPgammaS binding assay	[PMID: 31627993]
CHO-K1	EC₅₀	45.3 nM Compound: Serotonin	Partial agonist activity at recombinant human 5-HT1A expressed in CHO-K1 cells by pathhunter beta-arrestin assay Partial agonist activity at recombinant human 5-HT1A expressed in CHO-K1 cells by pathhunter beta-arrestin assay	[PMID: 32631554]
CHO-K1	EC₅₀	0.07 μM Compound: Serotonin	Agonist activity at human recombinant 5-HT7 receptor expressed in CHOK1 Hunter cell incubated for 30 to 60 mins by hit-hunter cAMP assay based chemiluminescence analysis Agonist activity at human recombinant 5-HT7 receptor expressed in CHOK1 Hunter cell incubated for 30 to 60 mins by hit-hunter cAMP assay based chemiluminescence analysis	[PMID: 32631561]
CHO-K1	EC₅₀	4.82 nM Compound: 5-HT	Inhibition of forskolin-stimulated c-AMP formation by human 5-hydroxytryptamine 1B receptor expressed in CHO-K1 cells Inhibition of forskolin-stimulated c-AMP formation by human 5-hydroxytryptamine 1B receptor expressed in CHO-K1 cells	[PMID: 8960551]
Flp-In-293	EC₅₀	0.19 nM Compound: Serotonin	Agonist activity at recombinant human unedited 5HT2C receptor isoform INI expressed in Flp-In-293 cells assessed as increase in calcium influx measured for 300 secs by Fluo-4 dye based FLIPR assay Agonist activity at recombinant human unedited 5HT2C receptor isoform INI expressed in Flp-In-293 cells assessed as increase in calcium influx measured for 300 secs by Fluo-4 dye based FLIPR assay	[PMID: 31445232]
Flp-In-293	EC₅₀	0.27 nM Compound: Serotonin	Agonist activity at recombinant human 5HT2A receptor expressed in Flp-In-293 cells assessed as increase in calcium influx measured for 300 secs by Fluo-4 dye based FLIPR assay Agonist activity at recombinant human 5HT2A receptor expressed in Flp-In-293 cells assessed as increase in calcium influx measured for 300 secs by Fluo-4 dye based FLIPR assay	[PMID: 31445232]
Flp-In-293	EC₅₀	0.86 nM Compound: Serotonin	Agonist activity at recombinant human 5HT2B receptor expressed in Flp-In-293 cells assessed as increase in calcium influx measured for 300 secs by Fluo-4 dye based FLIPR assay Agonist activity at recombinant human 5HT2B receptor expressed in Flp-In-293 cells assessed as increase in calcium influx measured for 300 secs by Fluo-4 dye based FLIPR assay	[PMID: 31445232]
HEK293	EC₅₀	0.5 μM Compound: 5-HT	Antagonist activity at human 5HT3A receptor expressed in HEK293 cells by FMP assay Antagonist activity at human 5HT3A receptor expressed in HEK293 cells by FMP assay	[PMID: 17722904]
HEK293	EC₅₀	0.1 μM Compound: serotonin	Activity at SER4 receptor expressed in HEK293 cells assessed as increase in calcium by calcium imaging assay Activity at SER4 receptor expressed in HEK293 cells assessed as increase in calcium by calcium imaging assay	[PMID: 18033297]
HEK293	EC₅₀	0.09 nM Compound: 1	Agonist activity at human 5HT2C receptor expressed in HEK293 cells assessed as calcium flux by FLIPR assay Agonist activity at human 5HT2C receptor expressed in HEK293 cells assessed as calcium flux by FLIPR assay	[PMID: 19284718]
HEK293	EC₅₀	0.5 nM Compound: 1	Agonist activity at human 5HT2B receptor expressed in HEK293 cells assessed as calcium flux by FLIPR assay Agonist activity at human 5HT2B receptor expressed in HEK293 cells assessed as calcium flux by FLIPR assay	[PMID: 19284718]
HEK293	EC₅₀	5.8 nM Compound: 1	Agonist activity at human 5HT2A receptor expressed in HEK293 cells assessed as calcium flux by FLIPR assay Agonist activity at human 5HT2A receptor expressed in HEK293 cells assessed as calcium flux by FLIPR assay	[PMID: 19284718]
HEK293	EC₅₀	141 nM Compound: 5-HT	Partial agonist activity at human 5HT3A receptor expressed in HEK293 cells assessed as decrease in 10 uM 5-chloroindole-induced increase in intracellular calcium release Partial agonist activity at human 5HT3A receptor expressed in HEK293 cells assessed as decrease in 10 uM 5-chloroindole-induced increase in intracellular calcium release	[PMID: 21146988]
HEK293	EC₅₀	178 nM Compound: 5-HT	Partial agonist activity at human 5HT3A receptor expressed in HEK293 cells assessed as effect on serotonin-induced response Partial agonist activity at human 5HT3A receptor expressed in HEK293 cells assessed as effect on serotonin-induced response	[PMID: 21146988]
HEK293	EC₅₀	0.09 nM Compound: 5-HT	Agonist activity at human 5HT2C receptor expressed in HEK293 cells assessed as calcium flux measured every sec for 3 mins by FLIPR assay Agonist activity at human 5HT2C receptor expressed in HEK293 cells assessed as calcium flux measured every sec for 3 mins by FLIPR assay	[PMID: 22778800]
HEK293	EC₅₀	0.86 nM Compound: 5-HT	Agonist activity at human 5HT2B receptor expressed in HEK293 cells assessed as calcium flux measured every sec for 3 mins by FLIPR assay Agonist activity at human 5HT2B receptor expressed in HEK293 cells assessed as calcium flux measured every sec for 3 mins by FLIPR assay	[PMID: 22778800]
HEK293	EC₅₀	7.6 nM Compound: 5-HT	Agonist activity at human 5HT2A receptor expressed in HEK293 cells assessed as calcium flux measured every sec for 3 mins by FLIPR assay Agonist activity at human 5HT2A receptor expressed in HEK293 cells assessed as calcium flux measured every sec for 3 mins by FLIPR assay	[PMID: 22778800]
HEK293	EC₅₀	0.0026 μM Compound: 5-HT, serotonin	Agonist activity at human 5HT2A receptor expressed in HEK293 cells assessed as induction of intracellular calcium release measured for 90 secs by fluorescence assay Agonist activity at human 5HT2A receptor expressed in HEK293 cells assessed as induction of intracellular calcium release measured for 90 secs by fluorescence assay	[PMID: 23301527]
HEK293	EC₅₀	0.044 μM Compound: 5-HT, serotonin	Agonist activity at human 5HT2C receptor expressed in HEK293 cells assessed as accumulation of IP1 incubated for 1 hr at 37 degC followed by 15 mins at RT by TR-FRET assay Agonist activity at human 5HT2C receptor expressed in HEK293 cells assessed as accumulation of IP1 incubated for 1 hr at 37 degC followed by 15 mins at RT by TR-FRET assay	[PMID: 23301527]
HEK293	EC₅₀	0.15 μM Compound: 5-HT, serotonin	Agonist activity at human 5HT2A receptor expressed in HEK293 cells assessed as accumulation of IP1 incubated for 1 hr at 37 degC followed by 15 mins at RT by TR-FRET assay Agonist activity at human 5HT2A receptor expressed in HEK293 cells assessed as accumulation of IP1 incubated for 1 hr at 37 degC followed by 15 mins at RT by TR-FRET assay	[PMID: 23301527]
HEK293	EC₅₀	0.00087 μM Compound: 5-HT, serotonin	Agonist activity at human 5HT2C receptor expressed in HEK293 cells assessed as induction of intracellular calcium release measured for 90 secs by fluorescence assay Agonist activity at human 5HT2C receptor expressed in HEK293 cells assessed as induction of intracellular calcium release measured for 90 secs by fluorescence assay	[PMID: 23301527]
HEK293	EC₅₀	0.87 nM Compound: 5-HT, serotonin	Agonist activity at human 5HT2C receptor expressed in HEK293 cells assessed as induction of intracellular calcium release measured for 90 secs by fluorescence assay Agonist activity at human 5HT2C receptor expressed in HEK293 cells assessed as induction of intracellular calcium release measured for 90 secs by fluorescence assay	[PMID: 23301527]
HEK293	EC₅₀	3.75 nM Compound: 5-HT	Agonist activity at 5-HT1A receptor (unknown origin) expressed in HEK293 cells assessed as inhibition of [35S]GTPgammaS binding by scintillation proximity assay Agonist activity at 5-HT1A receptor (unknown origin) expressed in HEK293 cells assessed as inhibition of [35S]GTPgammaS binding by scintillation proximity assay	[PMID: 23332346]
HEK293	EC₅₀	0.4 nM Compound: 5-HT	Agonist activity at human 5-HT2C receptor expressed in HEK293 cells assessed as increase in calcium mobilization by FLIPR assay Agonist activity at human 5-HT2C receptor expressed in HEK293 cells assessed as increase in calcium mobilization by FLIPR assay	[PMID: 23602445]
HEK293	EC₅₀	1.1 nM Compound: 5-HT	Agonist activity at human 5-HT2B receptor expressed in HEK293 cells assessed as increase in calcium mobilization by FLIPR assay Agonist activity at human 5-HT2B receptor expressed in HEK293 cells assessed as increase in calcium mobilization by FLIPR assay	[PMID: 23602445]
HEK293	EC₅₀	1.5 nM Compound: 5-HT	Agonist activity at human 5-HT2A receptor expressed in HEK293 cells assessed as increase in calcium mobilization by FLIPR assay Agonist activity at human 5-HT2A receptor expressed in HEK293 cells assessed as increase in calcium mobilization by FLIPR assay	[PMID: 23602445]
HEK293	EC₅₀	0.017 μM Compound: 5-HT, serotonin	Agonist activity at human 5-HT7 expressed in HEK-293 cells assessed as increase in cAMP level after 45 mins by HTRF assay Agonist activity at human 5-HT7 expressed in HEK-293 cells assessed as increase in cAMP level after 45 mins by HTRF assay	[PMID: 25073094]
HEK293	EC₅₀	0.21 nM Compound: 5-HT	Agonist activity at human 5-HT2C receptor expressed in HEK293 cells assessed as calcium flux by FLIPR assay Agonist activity at human 5-HT2C receptor expressed in HEK293 cells assessed as calcium flux by FLIPR assay	[PMID: 25633969]
HEK293	EC₅₀	0.92 nM Compound: 5-HT	Agonist activity at human 5-HT2B receptor expressed in HEK293 cells assessed as calcium flux by FLIPR assay Agonist activity at human 5-HT2B receptor expressed in HEK293 cells assessed as calcium flux by FLIPR assay	[PMID: 25633969]
HEK293	EC₅₀	1.88 nM Compound: 5-HT	Agonist activity at human 5-HT2A receptor expressed in HEK293 cells assessed as calcium flux by FLIPR assay Agonist activity at human 5-HT2A receptor expressed in HEK293 cells assessed as calcium flux by FLIPR assay	[PMID: 25633969]
HEK293	IC₅₀	1.8 x 10^-7 M Compound: Serotonin	Displacement of [3H]LSD from human recombinant 5-HT5A receptor expressed in HEK293 cells Displacement of [3H]LSD from human recombinant 5-HT5A receptor expressed in HEK293 cells	[PMID: 26988801]
HEK293	EC₅₀	5.8 nM Compound: 5-HT	Agonist activity at human 5-HT7R expressed in HEK293 cells assessed as induction of cAMP levels treated for 10 mins measured after 30 mins by D2-dye based fluorescence assay Agonist activity at human 5-HT7R expressed in HEK293 cells assessed as induction of cAMP levels treated for 10 mins measured after 30 mins by D2-dye based fluorescence assay	[PMID: 27475109]
HEK293	EC₅₀	5.8 x 10^-3 μM Compound: 5-HT	Agonist activity at human 5-HT7R expressed in HEK293 cells assessed as induction of cAMP levels treated for 10 mins measured after 30 mins by D2-dye based fluorescence assay Agonist activity at human 5-HT7R expressed in HEK293 cells assessed as induction of cAMP levels treated for 10 mins measured after 30 mins by D2-dye based fluorescence assay	[PMID: 27475109]
HEK293	IC₅₀	1.8 x 10^-7 M Compound: Serotonin	Displacement of [3H]LSD from human recombinant 5HT5A receptor expressed in HEK293 cells measured after 120 mins by scintillation counting method Displacement of [3H]LSD from human recombinant 5HT5A receptor expressed in HEK293 cells measured after 120 mins by scintillation counting method	[PMID: 27876250]
HEK293	IC₅₀	1.8 x 10^-1 μM Compound: Serotonin	Displacement of [3H]LSD from human recombinant 5HT5A receptor expressed in HEK293 cells measured after 120 mins by scintillation counting method Displacement of [3H]LSD from human recombinant 5HT5A receptor expressed in HEK293 cells measured after 120 mins by scintillation counting method	[PMID: 27876250]
HEK293	IC₅₀	260 nM Compound: Serotonin	Displacement of [3H]LSD from human recombinant 5-HT5a receptor in HEK293 cells after 120 mins by scintillation counting Displacement of [3H]LSD from human recombinant 5-HT5a receptor in HEK293 cells after 120 mins by scintillation counting	[PMID: 27997171]
HEK293	EC₅₀	0.17 nM Compound: 5-HT	Agonist activity at human 5-HT2C-INI receptor isoform expressed in Flp-IN HEK293 cells assessed as induction of Gq-mediated calcium flux measured every second for 5 mins by Fluo-4 dye based FLIPR assay Agonist activity at human 5-HT2C-INI receptor isoform expressed in Flp-IN HEK293 cells assessed as induction of Gq-mediated calcium flux measured every second for 5 mins by Fluo-4 dye based FLIPR assay	[PMID: 28657744]
HEK293	EC₅₀	1.46 nM Compound: 5-HT	Agonist activity at human 5-HT2B receptor expressed in Flp-IN HEK293 cells assessed as induction of calcium flux measured every second for 5 mins by Fluo-4 dye based FLIPR assay Agonist activity at human 5-HT2B receptor expressed in Flp-IN HEK293 cells assessed as induction of calcium flux measured every second for 5 mins by Fluo-4 dye based FLIPR assay	[PMID: 28657744]
HEK293	EC₅₀	2.35 nM Compound: 5-HT	Agonist activity at human 5-HT2A receptor expressed in Flp-IN HEK293 cells assessed as induction of calcium flux measured every second for 5 mins by Fluo-4 dye based FLIPR assay Agonist activity at human 5-HT2A receptor expressed in Flp-IN HEK293 cells assessed as induction of calcium flux measured every second for 5 mins by Fluo-4 dye based FLIPR assay	[PMID: 28657744]
HEK293	EC₅₀	0.41 nM Compound: 5-HT	Agonist activity at human 5HT2C receptor stably expressed in HEK293 cells co-expressing Gq assessed as induction of calcium mobilization measured at 1 sec interval for 60 secs by Fluo-4 calcium dye based FLIPR assay Agonist activity at human 5HT2C receptor stably expressed in HEK293 cells co-expressing Gq assessed as induction of calcium mobilization measured at 1 sec interval for 60 secs by Fluo-4 calcium dye based FLIPR assay	[PMID: 32282200]
HEK293-EBNA	EC₅₀	5.8 nM Compound: 5-HT	Agonist activity at human cloned 5HT2B receptor expressed in HEK293-EBNA cells assessed as myo-[3H]inositol hydrolysis Agonist activity at human cloned 5HT2B receptor expressed in HEK293-EBNA cells assessed as myo-[3H]inositol hydrolysis	[PMID: 18035544]
HRPE	IC₅₀	97 μM Compound: Serotonin	TP_TRANSPORTER: inhibition of MPP+ uptake (MPP+: 1 uM) in OCT3-expressing HRPE cells TP_TRANSPORTER: inhibition of MPP+ uptake (MPP+: 1 uM) in OCT3-expressing HRPE cells	[PMID: 9830022]
NG108-15	EC₅₀	298 nM Compound: 5-HT	Quantitative effect on the [14C]guanidinium accumulation in NG 108-15 cells. Quantitative effect on the [14C]guanidinium accumulation in NG 108-15 cells.	[PMID: 10229626]
NG108-15	EC₅₀	293 nM Compound: 5-HT	In Vitro 5-HT3 agonist activity on the [14C]guanidinium accumulation in NG 108-15 cells. In Vitro 5-HT3 agonist activity on the [14C]guanidinium accumulation in NG 108-15 cells.	[PMID: 9191957]
Oocyte	EC₅₀	12 μM Compound: Serotonin	Response at rat 5-hydroxytryptamine 3a receptor expressed in xenopus oocytes Response at rat 5-hydroxytryptamine 3a receptor expressed in xenopus oocytes	[PMID: 15050614]
U2OS	IC₅₀	1.25 nM Compound: Serotonin	Antagonist activity at recombinant human 5-HT2C expressed in human U2OS cells by pathhunter beta-arrestin assay Antagonist activity at recombinant human 5-HT2C expressed in human U2OS cells by pathhunter beta-arrestin assay	[PMID: 32631554]
U2OS	IC₅₀	27.9 nM Compound: Serotonin	Antagonist activity at recombinant human 5-HT2A expressed in human U2OS cells by pathhunter beta-arrestin assay Antagonist activity at recombinant human 5-HT2A expressed in human U2OS cells by pathhunter beta-arrestin assay	[PMID: 32631554]

In Vitro

Serotonin is a monoamine neurotransmitter in the CNS and an endogenous 5-HT receptor agonist. Serotonin also inhibits catechol O-methyltransferase (COMT), an enzyme that contributes to modultion the perception of pain, via non-competitive binding to the site bound by catechol substrates with a binding affinity comparable to the binding affinity of catechol itself (K_i= 44 μM). Results show that addition of 100 μM of Serotonin decreases the reaction velocity of COMT^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Serotonin produces robust hypersensitivity compare to saline-treated controls (p<0.001)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS. Nr. 50-67-9
Appearance Solid
Molecular Weight 176.22
Formel C₁₀H₁₂N₂O
Color Light yellow to brown
SMILES

OC1=CC2=C(NC=C2CCN)C=C1
Synonyms

5-Hydroxytryptamine; 5-HT
Structure Classification
- Alkaloids
- Indole Alkaloids
Initial Source
- Endogenous metabolite
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

-20°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Publications (41)

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Nature

Lithocholic acid phenocopies anti-ageing effects of calorie restriction. [Abstract]

2025 Jul;643(8070):192-200. PMID: 39695227
Cell Metab

2026 May 13:S1550-4131(26)00150-6. PMID: 42134325
Immunity

Spatiotemporal dynamics of CXCL10 encode contextual immune information revealed by the genetically encoded fluorescent sensor. [Abstract]

2025 Sep 9;58(9):2320-2335.e9. PMID: 40818452
Cell Res

Light sensing enhances thermotolerance and competitive fitness via serotonergic signaling in an eyeless organism. [Abstract]

2026 Apr;36(4):286-299. PMID: 41764323
Cell Stem Cell

Vagal pathway activation links chronic stress to decline in intestinal stem cell function. [Abstract]

2025 Mar 20:S1934-5909(25)00084-0. PMID: 40120585
Nat Commun

Gut microbiota modulates aversive learning and memory of honeybees (Apis mellifera). [Abstract]

2025 Dec 18;16(1):11196. PMID: 41413037
J Exp Clin Cancer Res

Mechanisms of neural infiltration-mediated tumor metabolic reprogramming impacting immunotherapy efficacy in non-small cell lung cancer. [Abstract]

2024 Oct 10;43(1):284. PMID: 39385213
Small

Acupuncture Mechanics, Stimulation, and Cellular Serotonin Detection by a Magneto-Responsive Nanomesh Sensor. [Abstract]

2026 Mar;22(14):e11914. PMID: 41537659
Cell Rep Med

5-HT reuptake blockade induces pyroptosis in BRAFV600E-mutated melanomas via remodeling histone serotonylation. [Abstract]

2026 Jan 20;7(1):102537. PMID: 41494533
J Immunother Cancer

GCH1 induces immunosuppression through metabolic reprogramming and IDO1 upregulation in triple-negative breast cancer. [Abstract]

2021 Jul;9(7):e002383. PMID: 34281987

Serotonin purchased from MedChemExpress. Usage Cited in: J Immunother Cancer. 2021 Jul;9(7):e002383. [Abstract]

Relative IDO1 transcription levels detected by quantitative reverse transcription PCR (qRT-PCR) analysis in BT549 cells treated with dimethyl sulfoxide (DMSO), tetrahydrobiopterin (BH4, 10 µM), 5-HTP (500 µM), serotonin (10 µM), NAS (100 µM), and melatonin (1 µM) for 24 hours.

Serotonin purchased from MedChemExpress. Usage Cited in: J Immunother Cancer. 2021 Jul;9(7):e002383. [Abstract]

Relative IDO1 levels detected by western blot in BT549 cells treated with dimethyl sulfoxide (DMSO), tetrahydrobiopterin (BH4, 10 µM), 5-HTP (500 µM), serotonin (10 µM), NAS (100 µM), and melatonin (1 µM) for 24 hours.
Acta Biomater

Sequential dual-drug delivery biomimetic hydrogel for temporal orchestration of acute neuroprotection and chronic regeneration in spinal cord injury. [Abstract]

2026 Mar:213:214-232. PMID: 41654155
Int J Biol Macromol

2026 Jun:369:152765. PMID: 42214532
Food Res Int

Polysaccharide from Aloe vera gel improves intestinal stem cells dysfunction to alleviate intestinal barrier damage via 5-HT. [Abstract]

2025 Aug:214:116675. PMID: 40467243
Curr Res Food Sci

Tryptophan metabolic pathway plays a key role in the stress-induced emotional eating. [Abstract]

2024 Apr 26:8:100754. PMID: 38736909

Serotonin purchased from MedChemExpress. Usage Cited in: Curr Res Food Sci. 2024 Apr 26:8:100754. [Abstract]

Dose-response and time course of cell viability after in vitro administration of 5-HT(Serotonin);
J Pineal Res

2026 Jul;78(4):e70154. PMID: 42262169
Neurosci Bull

Targeting 5-HT to Alleviate Dose-Limiting Neurotoxicity in Nab-Paclitaxel-Based Chemotherapy. [Abstract]

2025 Jul;41(7):1229-1245. PMID: 40369268
Mar Drugs

2026 Jun;24(6):189. PMID: 42346775
Eur J Pharmacol

2026 Jul 10:1029:178996. PMID: 42167669
iScience

5-HT promotes bronchopulmonary dysplasia via TGM2-mediated serotonylation. [Abstract]

2025 Nov 19;28(12):114120. PMID: 41438086

Serotonin purchased from MedChemExpress. Usage Cited in: iScience. 2025 Nov 19;28(12):114120. [Abstract]

Scratch tests were used to observe the migration ability of MLE-12 cells treated with 5-HT (Serotonin, 10 μM) for 24 h.
iScience

Deficiency of Rab26 causes behavioral defects in mice through impaired trafficking of serotonin (5-HT) transporter. [Abstract]

2025 Jun 18;28(7):112931. PMID: 40687824

Serotonin purchased from MedChemExpress. Usage Cited in: iScience. 2025 Jun 18;28(7):112931. [Abstract]

SH-SY5Y cells were treated with 10 μM Serotonin (5-HT), then the intracellular 5-HT was detected through ELISA assay for 0, 60, 120 min.
J Food Meas Charact

2026 Jan 30.
J Dent Sci

Serotonin promotes lip sensory recovery after inferior alveolar nerve transection via histone serotonylation. [Abstract]

2025 Oct;20(4):2363-2374. PMID: 41040553
Fitoterapia

The stem-derived bioactive fraction of Syringa oblata Lindl. Ameliorates insomnia via cAMP signaling-mediated regulation of neurotransmission, oxidative stress, and inflammation: A multi-omics study. [Abstract]

2026 Apr:190:107182. PMID: 41861857
Behav Brain Res

Serotonin mitigates depression in a rotenone-induced mouse Parkinson's disease model by inhibiting hippocampal neuronal pyroptosis and neuroinflammation. [Abstract]

2025 May 7:115620. PMID: 40345554
Biochem Biophys Res Commun

Glucose transports in the ileum: mechanism, regulation and physiological role of ileal glucose absorption. [Abstract]

2025 Sep 19:785:152676. PMID: 41005286
Indian J Ophthalmol

Ranitidine protects Müller cells against ferroptosis in diabetic retinopathy by regulating the AKT1/GSK3β pathway. [Abstract]

2026 Feb 1;74(2):250-258. PMID: 41581041
SSRN

2026 Jun 16.
bioRxiv

2026 May 29.
SSRN

2026 Apr 13.
SSRN

2026 Mar 6.
bioRxiv

2026 Mar 30.
bioRxiv

Quinone reductase 2 reads H3 serotonylation to support neuronal maturation. [Abstract]

2026 Mar 19:2026.03.17.712426. PMID: 41890023
bioRxiv

2025 Oct 14.
bioRxiv

2025 Sep 28.
SSRN

2025 Apr 24.
bioRxiv

2024 November 03.
bioRxiv

2024 November 03.
Res Sq

2024 Jun 11.
Research Square Preprint

2023 Dec 28.
bioRxiv

2023 Oct 13.
Authorea

September 19, 2022.

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (567.47 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

5.6747 mL

28.3736 mL

56.7472 mL

5 mM

1.1349 mL

5.6747 mL

11.3494 mL

10 mM

0.5675 mL

2.8374 mL

5.6747 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (14.19 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (14.19 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Protokoll

Cell Assay^[2]

For macrophage culture, peritoneal cavity cells are collected from WT and IL-13^-/- mice with or without dextran sodium sulfate (DSS) treatment and cultured. Cells are plated at a concentration of 3.0×10⁶ cells per millilitre, and treated with either lipopolysaccharides (LPS); (100 ng/mL) or Serotonin (10^-10 M) for 24 hours. The culture supernatant are collected and stored in -80°C until determination of cytokine levels using protein array system^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration^[2]

Dextran sodium sulfate (DSS) colitis is induced by orally administering 5% DSS in drinking water for 5 days. In a separate experiment, IL-13^-/- mice are injected subcutaneously with 100 mg/kg of Serotonin (5-HTP) twice daily for 8 days beginning 3 days prior to induction of DSS colitis; whereas, the control IL-13^-/- mice receive saline as vehicle. Animals are anaesthetized prior to euthanization via cervical dislocation at the conclusion of each experiment or if they reach a predetermined end point (ie, loss of ≥20% body weight and/or significant deterioration of body condition)^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (275 KB)
SDS (420 KB)
- English - EN (420 KB)
- Français - FR (420 KB)
- Deutsch - DE (420 KB)
- Norwegian - NO (420 KB)
- Español - ES (420 KB)
- Swedish - SV (420 KB)
- Italian - IT (420 KB)
- Korean - KR (420 KB)
- Portuguese - PT (420 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Tsao D, et al. Serotonin-induced hypersensitivity via inhibition of catechol O-methyltransferase activity. Mol Pain. 2012 Apr 13;8:25. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	5.6747 mL	28.3736 mL	56.7472 mL	141.8681 mL
	5 mM	1.1349 mL	5.6747 mL	11.3494 mL	28.3736 mL
	10 mM	0.5675 mL	2.8374 mL	5.6747 mL	14.1868 mL
	15 mM	0.3783 mL	1.8916 mL	3.7831 mL	9.4579 mL
	20 mM	0.2837 mL	1.4187 mL	2.8374 mL	7.0934 mL
	25 mM	0.2270 mL	1.1349 mL	2.2699 mL	5.6747 mL
	30 mM	0.1892 mL	0.9458 mL	1.8916 mL	4.7289 mL
	40 mM	0.1419 mL	0.7093 mL	1.4187 mL	3.5467 mL
	50 mM	0.1135 mL	0.5675 mL	1.1349 mL	2.8374 mL
	60 mM	0.0946 mL	0.4729 mL	0.9458 mL	2.3645 mL
	80 mM	0.0709 mL	0.3547 mL	0.7093 mL	1.7734 mL
	100 mM	0.0567 mL	0.2837 mL	0.5675 mL	1.4187 mL

Keywords

Serotonin

41 Cited Publications

100 mg Angebot einholen

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Serotonin

Publications Citing Use of MedChemExpress (MCE) Serotonin

Alle 5-HT Receptor Isoform-spezifische Produkte anzeigen

Alle Endogenous Metabolite Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (41)

Lösungsmittel & Löslichkeit

Protokoll

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Serotonin Related Classifications

HY-B1473A Related Classifications