1. Apoptosis
  2. Apoptosis
    Caspase
    Bcl-2 Family
  3. BBR-BODIPY

BBR-BODIPY 

Cat. No.: HY-147889
Handling Instructions

BBR-BODIPY is a fluorescent probe that allows screening its interaction with the targeted cells. BBR-BODIPY induces apoptosis and changes the expression of apoptosis-related proteins.

For research use only. We do not sell to patients.

BBR-BODIPY Chemical Structure

BBR-BODIPY Chemical Structure

CAS No. : 2456476-47-2

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Description

BBR-BODIPY is a fluorescent probe that allows screening its interaction with the targeted cells. BBR-BODIPY induces apoptosis and changes the expression of apoptosis-related proteins[1].

IC50 & Target

Bax

 

Caspase-9

 

In Vitro

BBR-BODIPY (1-32 μM; 24 hours; MCF7 and MDA-MB-231 cells) has anti-proliferative activity[1].
BBR-BODIPY (2-16 μM; 5-60 mins; MCF7 cells) could be quickly taken up into MCF7 cells in 5 mins and the fluorescence intensity exhibits a time-dependent manner[1].
BBR-BODIPY (1-32 μM; 24 hours; MCF7 and MDA-MB-231 cells) accumulates in mitochondria and overlaps with Mito tracker red to display yellow fluorescence with a Pearsons co-localization coefficient of 0.72[1].
BBR-BODIPY (8 μM, 12 hours) induces apoptosis in MCF7 cells through a mitochondrial pathway[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay[1]

Cell Line: MCF7 and MDA-MB-231 cells
Concentration: 1 , 2 , 4 , 8 , 16 and 32 μM
Incubation Time: 24 hours
Result: Inhibited with IC50 values of 40.81 and 41.46 μM for MCF7 and MDA-MB-231 cells, respectively.

Western Blot Analysis[1]

Cell Line: MCF7 cells
Concentration: 8 μM
Incubation Time: 12 hours
Result: The amounts of Bax and Cyto C released from mitochondria and cleaved Caspase 9 were up-regulated in MCF7 cells.
Molecular Weight

861.18

Formula

C47H48BClF2N6O5

CAS No.
SMILES

[F-][B+3]1([N]2=C(C)C=C(C)C2=C(C3=C(C)C=C(C)[N-]31)C4=CC=C(C=C4)OCCCCCCN5N=NC(COC6=C(C=CC7=CC8=[N+](C=C76)CCC9=CC%10=C(OCO%10)C=C89)OC)=C5)[F-].[Cl-]

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Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

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BBR-BODIPY
Cat. No.:
HY-147889
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