2. Apoptosis
  3. Apoptosis Caspase Bcl-2 Family
  4. BBR-BODIPY

BBR-BODIPY is a fluorescent probe that allows screening its interaction with the targeted cells. BBR-BODIPY induces apoptosis and changes the expression of apoptosis-related proteins.

For research use only. We do not sell to patients.

BBR-BODIPY Chemical Structure

BBR-BODIPY Chemical Structure

CAS No. : 2456476-47-2

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1 mg USD 120 In-stock
5 mg USD 276 In-stock
10 mg USD 420 In-stock
25 mg USD 778 In-stock
50 mg USD 1167 In-stock
100 mg USD 1750 In-stock
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Based on 1 publication(s) in Google Scholar

BBR-BODIPY Related Antibodies

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  • Biological Activity

  • Purity & Documentation

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  • Customer Review

Description

BBR-BODIPY is a fluorescent probe that allows screening its interaction with the targeted cells. BBR-BODIPY induces apoptosis and changes the expression of apoptosis-related proteins[1].

IC50 & Target

Bax

 

Caspase-9

 

In Vitro

BBR-BODIPY (1-32 μM; 24 hours; MCF7 and MDA-MB-231 cells) has anti-proliferative activity[1].
BBR-BODIPY (2-16 μM; 5-60 mins; MCF7 cells) could be quickly taken up into MCF7 cells in 5 mins and the fluorescence intensity exhibits a time-dependent manner[1].
BBR-BODIPY (1-32 μM; 24 hours; MCF7 and MDA-MB-231 cells) accumulates in mitochondria and overlaps with Mito tracker red to display yellow fluorescence with a Pearsons co-localization coefficient of 0.72[1].
BBR-BODIPY (8 μM, 12 hours) induces apoptosis in MCF7 cells through a mitochondrial pathway[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

BBR-BODIPY Related Antibodies

Cell Cytotoxicity Assay[1]

Cell Line: MCF7 and MDA-MB-231 cells
Concentration: 1 , 2 , 4 , 8 , 16 and 32 μM
Incubation Time: 24 hours
Result: Inhibited with IC50 values of 40.81 and 41.46 μM for MCF7 and MDA-MB-231 cells, respectively.

Western Blot Analysis[1]

Cell Line: MCF7 cells
Concentration: 8 μM
Incubation Time: 12 hours
Result: The amounts of Bax and Cyto C released from mitochondria and cleaved Caspase 9 were up-regulated in MCF7 cells.
Molecular Weight

861.18

Formula

C47H48BClF2N6O5
CAS No.
Appearance

Solid

Color

Orange to red

Emission (Em)

563
Excitation (Ex)

535
SMILES

[F-][B+3]1([N]2=C(C)C=C(C)C2=C(C3=C(C)C=C(C)[N-]31)C4=CC=C(C=C4)OCCCCCCN5N=NC(COC6=C(C=CC7=CC8=[N+](C=C76)CCC9=CC%10=C(OCO%10)C=C89)OC)=C5)[F-].[Cl-]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 62.5 mg/mL (72.57 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.1612 mL 5.8060 mL 11.6120 mL
5 mM 0.2322 mL 1.1612 mL 2.3224 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

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Concentration (final)

C2

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Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

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g

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(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.1612 mL 5.8060 mL 11.6120 mL 29.0299 mL
5 mM 0.2322 mL 1.1612 mL 2.3224 mL 5.8060 mL
10 mM 0.1161 mL 0.5806 mL 1.1612 mL 2.9030 mL
15 mM 0.0774 mL 0.3871 mL 0.7741 mL 1.9353 mL
20 mM 0.0581 mL 0.2903 mL 0.5806 mL 1.4515 mL
25 mM 0.0464 mL 0.2322 mL 0.4645 mL 1.1612 mL
30 mM 0.0387 mL 0.1935 mL 0.3871 mL 0.9677 mL
40 mM 0.0290 mL 0.1451 mL 0.2903 mL 0.7257 mL
50 mM 0.0232 mL 0.1161 mL 0.2322 mL 0.5806 mL
60 mM 0.0194 mL 0.0968 mL 0.1935 mL 0.4838 mL
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BBR-BODIPY Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

BBR-BODIPY2456476-47-2ApoptosisCaspaseBcl-2 Familyfluorescent probeanticancerapoptosisfluorescently labeled natural productInhibitorinhibitorinhibit

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