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Cat. No.: HY-189745

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HBP-10

HBP-10 is an anticancer agent containing a Michael acceptor. HBP-10 induces intracellular ROS accumulation, triggering IRE1α-JNK/CHOP-mediated ER stress, γ-H2AX-positive DNA damage, and caspase-dependent apoptosis (apoptosis). HBP-10's above effects are attenuated by NAC (HY-B0215). HBP-10 exerts antiproliferative and cytotoxic effects on NSCLC cells and shows certain selectivity for NSCLC cells. HBP-10 can be used for research on EGFR-mutant non-small cell lung cancer.

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Formule: C₁₄H₇ClN₄O₂
Masse moléculaire:298.68

Stockage:

Please store the product under the recommended conditions in the Certificate of Analysis.

HBP-10 Chemical Structure

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Activité biologique
- Description
- Cellular Effect
- In Vitro
Chemical Information
Pureté et documentation
Références
Calculators
Help & FAQs
Classifications
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Activité biologique

Description

Voir tous les produits spécifiques à Isoform JNK :

Voir tous les produits spécifiques à Isoform Caspase :

Cellular Effect

Cell Line	Type	Value	Description	References
H1975	IC50	45.15 μM	Inhibition of viability in human H1975 cells by CCK-8 assay after incubation with HBP-10 for 24 hrs. Inhibition of viability in human H1975 cells by CCK-8 assay after incubation with HBP-10 for 24 hrs.	42697283
HCC827	IC50	69.54 μM	Inhibition of viability in human HCC827 cells by CCK-8 assay after incubation with HBP-10 for 24 hrs. Inhibition of viability in human HCC827 cells by CCK-8 assay after incubation with HBP-10 for 24 hrs.	42697283
A549	IC50	58.76 μM	Inhibition of viability in human A549 cells by CCK-8 assay after incubation with HBP-10 for 24 hrs. Inhibition of viability in human A549 cells by CCK-8 assay after incubation with HBP-10 for 24 hrs.	42697283
HEK293	IC50	>200 μM	Inhibition of viability in human HEK293 cells by CCK-8 assay after incubation with HBP-10 for 24 hrs with IC50 >200 μM. Inhibition of viability in human HEK293 cells by CCK-8 assay after incubation with HBP-10 for 24 hrs with IC50 >200 μM.	42697283

In Vitro

Anti-cancerOxidative StressEndoplasmic Reticulum StressApoptosis

Anti-cancer

HBP-10 (0-40 μM; 7 days) inhibits the long-term clonogenic survival of H1975 NSCLC cells in a dose-dependent manner^[1].
HBP-10 (24 h) inhibits cell viability, with an IC₅₀ of 45.15 μM in H1975 cells, 69.54 μM in HCC827 cells, 58.76 μM in A549 cells, and >200 μM in HEK293 cells^[1].
HBP-10 (50 μM) induces DNA damage signaling in H1975 cells, as evidenced by increased γ-H2AX^[1].
HBP-10 (50 μM) reduces viable H1975 cells and increases PI-positive and calcein-negative damaged cells^[1].

Oxidative Stress

HBP-10 (50 μM) induces ROS accumulation in H1975 cells^[1].

Endoplasmic Reticulum Stress

HBP-10 (50 μM; 0-12 h) activates UPR-related ER stress signaling in H1975 cells^[1].

Apoptosis

HBP-10 (50 μM; 24 h) induces apoptotic nuclear morphology in H1975 cells^[1].
HBP-10 (0-100 μM; 0-24 h) activates caspase-dependent apoptotic signaling in H1975 cells^[1].

Oxidative Stress

Endoplasmic Reticulum Stress

Apoptosis

HBP-10-induced ER stress, DNA damage, and apoptotic signaling in H1975 cells are inhibited by NAC (HY-B0215)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:

H1975 NSCLC cells
Concentration:

0, 10, 20 and 40 μM
Incubation Time:

7 days
Result:

Inhibited long-term colony-forming survival of H1975 NSCLC cells in a dose-dependent manner.

Western Blot Analysis^[1]

Cell Line:

H1975 cells
Concentration:

50 μM
Incubation Time:

0, 0.5, 1, 3, 6, 12 h
Result:

Exposure to this compound increased BiP/GRP78 expression, enhanced IRE1α phosphorylation and accumulation, increased JNK phosphorylation, and elevated CHOP expression after 6 h.

Apoptosis Analysis^[1]

Cell Line:

H1975 cells
Concentration:

50 μM
Incubation Time:

24 h
Result:

Induced extensive chromatin condensation and nuclear fragmentation.

Western Blot Analysis^[1]

Cell Line:

H1975 cells
Concentration:

0, 10, 50, 100 μM
Incubation Time:

0, 6, 12, 24 h
Result:

Exposure to this compound induced cleavage of pro-caspase-2 and accumulation of cleaved caspase-7, cleaved caspase-3, and cleaved PARP.
Accumulation of cleaved caspase-7, cleaved caspase-3, and cleaved PARP was particularly evident after 24 h of exposure.

Related Protocols :

Chemical Information

Masse moléculaire 298.68
Formule C₁₄H₇ClN₄O₂
SMILES

OC1=C(C(C(C=N2)=CN3C2=C(C#N)C=N3)=O)C=C(Cl)C=C1
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage

Please store the product under the recommended conditions in the Certificate of Analysis.

Pureté et documentation

Instruction de manipulation (2659 KB)

Références

[1]. Baek JY, et al. 6-(2-Hydroxybenzoyl)pyrazolo[1,5-a]pyrimidine-3‑carbonitriles as novel reactive oxygen species-generating anticancer agents inducing ER stress and DNA damage in EGFR-mutant non-small cell lung cancer. Bioorg Med Chem Lett. 2026 Sep 4;142:130775.

Calculators

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Calculateur de dilution

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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MOQ

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100 mg

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Activité biologique
- Description
- In Vitro
Chemical Information
Pureté et documentation
Références

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HBP-10

Voir tous les produits spécifiques à Isoform JNK

Voir tous les produits spécifiques à Isoform Caspase