Cat. No.: HY-B0026

Ceftiofur hydrochloride

4 Cited Publications

Customer Review

Based on 4 publication(s) in Google Scholar

Ceftiofur hydrochloride is a cell wall synthesis inhibitor that targets bacterial penicillin-binding proteins (PBPs) and has anti-inflammatory effects in endotoxemia. Ceftiofur hydrochloride exerts bactericidal effects by inhibiting the synthesis of bacterial cell wall peptidoglycan, leading to bacterial cell lysis. Ceftiofur hydrochloride also inhibits the activation of NF-κB and MAPKs, thereby reducing the secretion of pro-inflammatory cytokines such as TNF-α, IL-1β, and IL-6.

For research use only. We do not sell to patients.

Purity: 98.43%
CAS No.: 103980-44-5
Formula: C₁₉H₁₈ClN₅O₇S₃
Molecular Weight:560.02

Storage:

-20°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Ceftiofur hydrochloride Chemical Structure

CAS No.: 103980-44-5

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Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (4)
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

bacterial^[1]

All Antibiotic Isoforms :

All NF-κB Isoforms :

All p38 MAPK Isoforms :

All TNF Receptor Isoforms :

All Interleukin Related Isoforms :

In Vitro

Ceftiofur (1, 5, 10 mg/L; pretreatment for 1 h+LPS stimulation for 12 h) hydrochloride can significantly inhibit the secretion of TNF-α, IL-1β, and IL-6 in RAW 264.7 cells, but has no significant effect on the secretion of IL-10^[2].
Ceftiofur hydrochloride (1, 5, 10 mg/L; pretreatment for 1 h+LPS stimulation for 30 min) can significantly inhibit the phosphorylation of ERK, JNK, and p38 and the nuclear translocation of NF-κB p65 in RAW 264.7 cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[2]

Cell Line:

RAW 264.7 cells
Concentration:

1, 5, 10 mg/L
Incubation Time:

1 h pretreatment with Ceftiofur followed by 30 min LPS stimulation
Result:

Dramatically decreased the phosphorylation levels of ERK, JNK, and p38 in Ceftiofur-treated cells compared to the LPS-treated control cells, while the total protein levels of MAPKs showed no change.

Immunofluorescence^[2]

Cell Line:

RAW 264.7 cells
Concentration:

1, 5, 10 mg/L
Incubation Time:

1 h pretreatment with Ceftiofur followed by 30 min LPS stimulation
Result:

Nuclear translocation of NF-κB p65 induced by LPS was strongly inhibited in a dose-dependent manner in Ceftiofur-pretreated cells.

In Vivo

Ceftiofur (20 mg/kg; sc; single dose; 1 h before or 0/1 h after 30 mg/kg LPS challenge) hydrochloride significantly improves the survival rate of mice in the endotoxemia model of female C57BL/6 mice, and reduces the levels of plasma TNF-α, IL-1β, and IL-6, and increases the level of IL-10^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Female C57BL/6 mice (18-20 g, 6-8 weeks old), LPS-induced endotoxemia model^[3]
Dosage:

20 mg/kg Ceftiofur (dissolved in saline)
Administration:

Subcutaneous injection, single dose, administered 1 h before or 0, 1, 4, 12 h after intraperitoneal injection of 30 mg/kg LPS.
Result:

Pretreatment with 20 mg/kg Ceftiofur 1 h before LPS challenge significantly increased the survival rate of mice (70% survival at 144 h vs. 0% in LPS control group).
When administered 0 or 1 h after LPS injection, Ceftiofur still provided significant protection, but protection decreased when delayed to 4 h and was absent at 12 h.
Plasma levels of TNF-α, IL-1β, and IL-6 in Ceftiofur-pretreated mice were significantly lower than those in the LPS control group at specific time points (1 h for TNF-α, 3 h for IL-6, 12 h for IL-1β), while IL-10 levels were significantly higher at 1 h after LPS challenge.

Chemical Information

CAS No. 103980-44-5
Appearance Solid
Molecular Weight 560.02
Formula C₁₉H₁₈ClN₅O₇S₃
Color White to light yellow
SMILES

[H]Cl.O=C(C(N12)=C(CSC(C3=CC=CO3)=O)CS[C@]2([H])[C@H](NC(/C(C4=CSC(N)=N4)=N\OC)=O)C1=O)O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Publications (4)

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Journal Impact Factor

Nat Commun

Selective inhibition of cancer cell self-renewal through a Quisinostat-histone H1.0 axis. [Abstract]

2020 Apr 14;11(1):1792. PMID: 32286289
Mol Syst Biol

Benchmarking AlphaFold-enabled molecular docking predictions for antibiotic discovery. [Abstract]

2022 Sep;18(9):e11081. PMID: 36065847
Microorganisms

Isolation, Antimicrobial Susceptibility, and Genotypes of Three Pasteurellaeae Species Prevalent on Pig Farms in China Between 2021 and 2023. [Abstract]

2025 Apr 18;13(4):938. PMID: 40284774
Vet Microbiol

Discovery of the tigecycline resistance gene cluster tmexCD3-toprJ1 in Pasteurella multocida strains isolated from pigs in China. [Abstract]

2024 May:292:110046. PMID: 38471428

Solvent & Solubility

In Vitro:

DMSO : 175 mg/mL (312.49 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.7857 mL

8.9283 mL

17.8565 mL

5 mM

0.3571 mL

1.7857 mL

3.5713 mL

10 mM

0.1786 mL

0.8928 mL

1.7857 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (282 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Salmon SA, et al. In vitro activity of Ceftiofur and its primary metabolite, desfuroylCeftiofur, against organisms of veterinary importance. J Vet Diagn Invest. 1996 Jul;8(3):332-6. [Content Brief]

[2]. Ci X, et al. Ceftiofur impairs pro-inflammatory cytokine secretion through the inhibition of the activation of NF-kappaB and MAPK. Biochem Biophys Res Commun. 2008 Jul 18;372(1):73-7. [Content Brief]

[3]. Ci X, et al. Ceftiofur regulates LPS-induced production of cytokines and improves LPS-induced survival rate in mice. Inflammation. 2008 Dec;31(6):422-7. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.7857 mL	8.9283 mL	17.8565 mL	44.6413 mL
	5 mM	0.3571 mL	1.7857 mL	3.5713 mL	8.9283 mL
	10 mM	0.1786 mL	0.8928 mL	1.7857 mL	4.4641 mL
	15 mM	0.1190 mL	0.5952 mL	1.1904 mL	2.9761 mL
	20 mM	0.0893 mL	0.4464 mL	0.8928 mL	2.2321 mL
	25 mM	0.0714 mL	0.3571 mL	0.7143 mL	1.7857 mL
	30 mM	0.0595 mL	0.2976 mL	0.5952 mL	1.4880 mL
	40 mM	0.0446 mL	0.2232 mL	0.4464 mL	1.1160 mL
	50 mM	0.0357 mL	0.1786 mL	0.3571 mL	0.8928 mL
	60 mM	0.0298 mL	0.1488 mL	0.2976 mL	0.7440 mL
	80 mM	0.0223 mL	0.1116 mL	0.2232 mL	0.5580 mL
	100 mM	0.0179 mL	0.0893 mL	0.1786 mL	0.4464 mL

Keywords

Ceftiofur hydrochloride

4 Cited Publications

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Ceftiofur hydrochloride

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Publications Citing Use of MedChemExpress (MCE) Ceftiofur hydrochloride

All Antibiotic Isoforms

All NF-κB Isoforms

All p38 MAPK Isoforms

All TNF Receptor Isoforms

All Interleukin Related Isoforms

Biological Activity

Chemical Information

Publications (4)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Ceftiofur hydrochloride Related Classifications

HY-B0026 Related Classifications