Art. -Nr.: HY-N6687

Calcimycin

(Synonyms: A-23187; Antibiotic A-23187)

26 Cited Publications

Kundenbewertung

Based on 26 publication(s) in Google Scholar

Calcimycin (A-23187) is an antibiotic and a unique divalent cation ionophore (like calcium and magnesium). Calcimycin induces Ca²⁺-dependent cell death by increasing intracellular calcium concentration. Calcimycin inhibits the growth of Gram-positive bacteria and some fungi. Calcimycin also inhibits the activity of ATPase and uncouples oxidative phosphorylation (OXPHOS) of mammalian cells. Calcimycin induces apoptosis and autophagy.

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Reinheit: 99.10%
CAS. Nr.: 52665-69-7
Formel: C₂₉H₃₇N₃O₆
Molecular Weight:523.62

Speicherung:

4°C, sealed storage, away from moisture

* In solvent : -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture)

Calcimycin Chemical Structure

CAS. Nr.: 52665-69-7

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Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- In Vitro
- In Vivo
- Clinical Trial
Chemical Information
Publications (26)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Alle Antibiotic Isoform-spezifische Produkte anzeigen :

In Vitro

Calcimycin (A-23187) mediates mycobacterial killing by inducing intracellular calcium-regulated autophagy in a P2RX7 dependent manner^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Calcimycin (A-23187) (2.5 or 7.5 nM; intrapleurally) induces protein leakage^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Mice (ICR, 25-30 g)^[5]
Dosage:

2.5 or 7.5 nM
Administration:

Intrapleurally
Result:

Two hours after 2.5 nM, or three hours after 7.5 nM, challenge the protein levels in the pleural cavity were equivalent to about a half of their corresponding peak values.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS. Nr. 52665-69-7
Appearance Solid
Molecular Weight 523.62
Formel C₂₉H₃₇N₃O₆
Color White to off-white
SMILES

O=C(C1=C2N=C(C[C@H]3O[C@]4(O[C@H]([C@H](C)C(C5=CC=CN5)=O)[C@H](C)C[C@H]4C)CC[C@H]3C)OC2=CC=C1NC)O
Synonyms

A-23187; Antibiotic A-23187
Structure Classification
- Antibiotics
- Polyether Antibiotics
Initial Source
- Microorganisms
Streptomyces chartreusensis
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

4°C, sealed storage, away from moisture

* In solvent : -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture)

Publications (26)

All WBIPIFCell Proliferation/Viability Assay

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Journal Impact Factor

Cancer Commun (Lond)

Cytosolic TGM2 promotes malignant progression in gastric cancer by suppressing the TRIM21-mediated ubiquitination/degradation of STAT1 in a GTP binding-dependent modality. [Abstract]

2023 Jan;43(1):123-149. PMID: 36353796

Calcimycin purchased from MedChemExpress. Usage Cited in: Cancer Commun (Lond). 2023 Jan;43(1):123-149. [Abstract]

Cells were treated with different concentrations of Calcimycin (A23187: 2 µmol/L, 4 µmol/L, 8 µmol/L, 10 µmol/L; 24 h), and the effect of A23187 on STAT1 expression was assessed by Western blotting.

Calcimycin purchased from MedChemExpress. Usage Cited in: Cancer Commun (Lond). 2023 Jan;43(1):123-149. [Abstract]

Cells were treated with A23187 (4 µmol/L; 24 h) to examine the effect of A21187 on the binding ability of TRIM21 and STAT1 by Co‐IP and western blotting, and the result showed that A23187 reversed the dissociation of TRIM21 and STAT1 in the context of TGM2 and increased ubiquitination of STAT1.

Calcimycin purchased from MedChemExpress. Usage Cited in: Cancer Commun (Lond). 2023 Jan;43(1):123-149. [Abstract]

The effect of A23187 (4 µmol/L; 0-96 h) and ZM39923 (20 nmol/L) on GC cell proliferation was evaluated by CCK8 assay. The result showed that ZM39923 promoted cell proliferation while A23187 had an inhibitory role in GC cell proliferation.
Adv Sci (Weinh)

Succinate Dehydrogenase Subunit A (SDHA) Mediated Microglia Extracellular Traps Formation Participating in Cerebral Ischemic Reperfusion Injury. [Abstract]

2025 Aug 20:e11873. PMID: 40832885

Calcimycin purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Aug 20:e11873. [Abstract]

A23187 (5 µM; 1–6 h) induced apoptosis in microglia in vitro. Microglia viability was assessed using the CCK-8 assay.

Calcimycin purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Aug 20:e11873. [Abstract]

A23187 (5 µM; 4 h). Representative images of microglia stained with Iba1, citH3, and MPO triple staining, and colocalization analysis of MPO, citH3, and DNA in the three cell groups.
Mol Ther

mmu-lncRNA 121686/hsa-lncRNA 520657 induced by METTL3 drive the progression of AKI by targeting miR-328-5p/HtrA3 signaling axis. [Abstract]

2022 Dec 7;30(12):3694-3713. PMID: 35869629
Brain

Hippocampal excitation-inhibition balance underlies the 5-HT2C receptor in modulating depressive behaviours. [Abstract]

2024 Nov 4;147(11):3764-3779. PMID: 38701344
Proc Natl Acad Sci U S A

Activation of WNK1 signaling through Piezo1. [Abstract]

2025 Sep 2;122(35):e2513155122. PMID: 40880539
Int J Biol Macromol

FKBP5 inhibitor suppresses platelet activation and thrombosis by inhibiting IKBKE/PI3K/Rap1 pathway. [Abstract]

2025 May 7:144068. PMID: 40345291
Phytomedicine

Sinapine targeting PLCβ3 EF hands disrupts Gαq-PLCβ3 interaction and ameliorates cardiovascular diseases. [Abstract]

2024 Apr:126:155200. PMID: 38387273
EMBO Mol Med

Conformational change of adenine nucleotide translocase-1 mediates cisplatin resistance induced by EBV-LMP1. [Abstract]

2021 Dec 7;13(12):e14072. PMID: 34755470
ACS Appl Mater Interfaces

2026 Apr 22;18(15):21604-21621. PMID: 41972905
Chemosphere

PCB126 impairs human sperm functions by affecting post-translational modifications and mitochondrial functions. [Abstract]

2024 Jan:346:140532. PMID: 37918541
J Med Chem

Optical Control of Leukotriene A4 Hydrolase Using Photoswitchable Inhibitors. [Abstract]

2025 Oct 23;68(20):21520-21533. PMID: 41041960
Antioxidants (Basel)

Muscone and (+)-Borneol Cooperatively Strengthen CREB Induction of Claudin 5 in IL-1 β-Induced Endothelium Injury. [Abstract]

2022 Jul 26;11(8):1455. PMID: 35892657
EMBO Rep

Amino acid starvation-induced LDLR trafficking accelerates lipoprotein endocytosis and LDL clearance. [Abstract]

2022 Feb 3;23(3):e53373. PMID: 34994492
Ecotoxicol Environ Saf

HFPO homologues, the novel alternatives to PFOA, impair male reproduction in mice through inducing mitochondrial dysfunction. [Abstract]

2025 Jun 2:300:118435. PMID: 40460775
Virulence

Staphylococcus aureus β-hemolysin impairs oxygen transport without causing hemolysis. [Abstract]

2025 Dec;16(1):2490208. PMID: 40202859
Life Sci

MiR-6918-5p prevents renal tubular cell apoptosis by targeting MBD2 in ischemia/reperfusion-induced AKI. [Abstract]

2022 Nov 1:308:120921. PMID: 36057400
PLoS Pathog

LL-37 selectively targets Plasmodium-infected erythrocytes and exhibits antimalarial activity. [Abstract]

2026 Mar 17;22(3):e1014062. PMID: 41843625
Mbio

Calmodulin Binding Activates Chromobacterium CopC Effector to ADP-Riboxanate Host Apoptotic Caspases. [Abstract]

2022 Apr 21;e0069022. PMID: 35446120
J Proteome Res

2026 Feb 25;25(3):1584-1600.
Exp Cell Res

Septins regulate kinase-inhibitor induced micron-scale vacuolation. [Abstract]

2026 Aug 1;461(1):115103. PMID: 42276196
Life Metab

Energy stress-induced PKCζ S-glutathionylation is essential for LKB1 cytoplasmic translocation and AMPK activation. [Abstract]

2025 Jul 16;4(6):loaf027. PMID: 41070197
BMC Cancer

TRPM2 channels mediate ROS-induced actin remodeling and cell migration of prostate cancer cells. [Abstract]

2025 May 28;25(1):956. PMID: 40437388
Thromb J

Endoplasmic reticulum protein 29 negatively regulates platelet functions and thrombosis in mice. [Abstract]

2025 May 7;23(1):44. PMID: 40336049
Res Sq

2026 Feb 12.
Cell Biomater

2026 Jan 12.
bioRxiv

2025 May 05.

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 33.33 mg/mL (63.65 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.9098 mL

9.5489 mL

19.0978 mL

5 mM

0.3820 mL

1.9098 mL

3.8196 mL

10 mM

0.1910 mL

0.9549 mL

1.9098 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (4.77 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (4.77 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (277 KB)
SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Wu Q, et al. Characterization of the biosynthesis gene cluster for the pyrrole polyether antibiotic calcimycin(A23187) in Streptomyces chartreusis NRRL 3882. Antimicrob Agents Chemother. 2011 Mar;55(3):974-82. [Content Brief]

[2]. Elliott JI, et al. IKCa1 activity is required for cell shrinkage, phosphatidylserine translocation and death in Tlymphocyte apoptosis. EMBO Rep. 2003 Feb;4(2):189-94. [Content Brief]

[3]. Engedal N, et al. Modulation of intracellular calcium homeostasis blocks autophagosome formation. Autophagy. 2013 Oct;9(10):1475-90. [Content Brief]

[4]. Mawatwal S, et al. Calcimycin mediates mycobacterial killing by inducing intracellular calcium-regulated autophagy in a P2RX7 dependent manner. Biochim Biophys Acta Gen Subj. 2017 Dec;1861(12):3190-3200. [Content Brief]

[5]. Wang JP, et al. Effect of norathyriol, isolated from Tripterospermum lanceolatum, on A23187-induced pleurisy and analgesia in mice. Naunyn Schmiedebergs Arch Pharmacol. 1994 Jul;350(1):90-5. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.9098 mL	9.5489 mL	19.0978 mL	47.7445 mL
	5 mM	0.3820 mL	1.9098 mL	3.8196 mL	9.5489 mL
	10 mM	0.1910 mL	0.9549 mL	1.9098 mL	4.7745 mL
	15 mM	0.1273 mL	0.6366 mL	1.2732 mL	3.1830 mL
	20 mM	0.0955 mL	0.4774 mL	0.9549 mL	2.3872 mL
	25 mM	0.0764 mL	0.3820 mL	0.7639 mL	1.9098 mL
	30 mM	0.0637 mL	0.3183 mL	0.6366 mL	1.5915 mL
	40 mM	0.0477 mL	0.2387 mL	0.4774 mL	1.1936 mL
	50 mM	0.0382 mL	0.1910 mL	0.3820 mL	0.9549 mL
	60 mM	0.0318 mL	0.1591 mL	0.3183 mL	0.7957 mL

Calcimycin

26 Cited Publications

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Calcimycin

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Publications Citing Use of MedChemExpress (MCE) Calcimycin

Alle Antibiotic Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (26)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Calcimycin Related Classifications

HY-N6687 Related Classifications