Segetalin B
Based on 2 publication(s) in Google Scholar
Segetalin B, an orally active cyclopentapeptide found in Vaccaria segetalis, possesses estrogen-like activity. Segetalin B promotes mineralization of ovariectomized rat-derived bone marrow mesenchymal stem cells (BMSCs) in vitro and increases the level of osteocalcin, BMP-2, ALP, and SIRT1 activity. Segetalin B is promising for research of post-menopausal osteoporosis (PMOP).
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- Pureté: 99.56%
- CAS No.: 164991-89-3
- Formule: C24H32N6O5
- Masse moléculaire:484.55
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Stockage:
Sealed storage, away from moisture and light.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) Segetalin B
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Activité biologique
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SIRT1 |
RUNX2 |
Segetalin B (0.1-100 μM, 24 h) shows significant cytotoxicity at 100 μM[4].
Segetalin B (0.1-10 μM, 15 days) enhances the mineralization of ovariectomized rat-derived BMSCs and increases ALP activity and levels of Osteocalcin and BMP-2[4].
Segetalin B (10 μM, 15 days) upregulates the protein expressions of Runx2 and osterix in BMSCs[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Ovariectomized rat‐derived BMSCs
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Concentration:10 μM
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Incubation Time:15 days
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Result:Significantly upregulated the expression levels of Runx2 and Osterix within cells.
Segetalin B (10-160 mg/kg, p.o., daily for 4 weeeks) inhibits bone loss in ovariectomized rats and upregulates expressions of Runx2, osterix, and SIRT1, and downregulates expressions of NICD and Hes1 in bone tissue of ovariectomized rats[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Ovariectomized rats[1]
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Dosage:2.5 mg/kg
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Administration:s.c., daily for two weeks
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Result:Increased the uterine weight to 75.6±8.87 mg in ovariectomized rats.
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Animal Model:Ovariectomized rats[4]
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Dosage:10-160 mg/kg
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Administration:p.o., daily for 4 weeeks
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Result:Significantly increased the calcium and phosphorus content in the urine of ovariectomized rats and upregulated the protein expressions of Runx2, Osterix, and SIRT1 in the corresponding region.
Chemical Information
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CAS No. 164991-89-3
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Appearance Solid
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Masse moléculaire 484.55
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Formule C24H32N6O5
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Color White to off-white
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SMILES
O=C(N[C@H](C(NCC(N[C@H](C(N[C@H](C(N1)=O)C)=O)C(C)C)=O)=O)C)[C@@H]1CC2=CNC3=CC=CC=C23
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Sequence
Cyclo(Ala-Gly-Val-Ala-Trp)
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Sequence Shortening
Cyclo(AGVAW)
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Structure Classification
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Initial Source
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Sealed storage, away from moisture and light
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (2)
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Journal Impact Factor
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Most Recent
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Drug Dev Res
SIRT1/Notch1 signal axis involves in the promoting effect of Segetalin B on bone formation. [Abstract]2022 Dec;83(8):1845-1857. PMID: 36207817 -
J Steroid Biochem Mol Biol
Segetalin B promotes bone formation in ovariectomized mice by activating PLD1/SIRT1 signaling to inhibit γ-secretase-mediated Notch1 overactivation. [Abstract]2025 Mar:247:106669. PMID: 39736459
Solvant et solubilité
DMSO : 250 mg/mL (515.94 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (4.29 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (4.29 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureté et documentation
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Fiche technique (285 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
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Instruction de manipulation (2659 KB)
Références
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.0638 mL | 10.3189 mL | 20.6377 mL | 51.5943 mL |
| 5 mM | 0.4128 mL | 2.0638 mL | 4.1275 mL | 10.3189 mL | |
| 10 mM | 0.2064 mL | 1.0319 mL | 2.0638 mL | 5.1594 mL | |
| 15 mM | 0.1376 mL | 0.6879 mL | 1.3758 mL | 3.4396 mL | |
| 20 mM | 0.1032 mL | 0.5159 mL | 1.0319 mL | 2.5797 mL | |
| 25 mM | 0.0826 mL | 0.4128 mL | 0.8255 mL | 2.0638 mL | |
| 30 mM | 0.0688 mL | 0.3440 mL | 0.6879 mL | 1.7198 mL | |
| 40 mM | 0.0516 mL | 0.2580 mL | 0.5159 mL | 1.2899 mL | |
| 50 mM | 0.0413 mL | 0.2064 mL | 0.4128 mL | 1.0319 mL | |
| 60 mM | 0.0344 mL | 0.1720 mL | 0.3440 mL | 0.8599 mL | |
| 80 mM | 0.0258 mL | 0.1290 mL | 0.2580 mL | 0.6449 mL | |
| 100 mM | 0.0206 mL | 0.1032 mL | 0.2064 mL | 0.5159 mL |
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