Cat. No.: HY-122942

Moracin M

4 Cited Publications

Customer Review

Based on 4 publication(s) in Google Scholar

Moracin M is a phenolic component that can be isolated from Mori Cortex, is a potent phosphodiesterase-4 (PDE4) inhibitor with IC₅₀ values of 2.9, 4.5, >40, and >100 μM for PDE4D2, PDE4B2, PDE5A1, and PDE9A2, respectively. Moracin M has anti-inflammatory activity.

For research use only. We do not sell to patients.

Purity: 98.99%
CAS No.: 56317-21-6
Formula: C₁₄H₁₀O₄
Molecular Weight:242.23

Storage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Moracin M Chemical Structure

CAS No.: 56317-21-6

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (4)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

IC₅₀ & Target^[1]

PDE4D2

2.9 μM (IC₅₀)

PDE4B2

4.5 μM (IC₅₀)

PDE5A1

>40 μM (IC₅₀)

PDE9A2

>100 μM (IC₅₀)

All Phosphodiesterase (PDE) Isoforms :

All Endogenous Metabolite Isoforms :

All NF-κB Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
BV-2	EC₅₀	15.1 μM Compound: moracin M	Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-stimulated NO production after 24 hrs by Griess assay Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-stimulated NO production after 24 hrs by Griess assay	[PMID: 26473791]
C8166	CC₅₀	57.4 μg/mL Compound: 7	Cytotoxicity against human C8166 cells assessed as inhibition of cell viability after 3 days by MTT assay Cytotoxicity against human C8166 cells assessed as inhibition of cell viability after 3 days by MTT assay	[PMID: 23368966]
C8166	EC₅₀	4.59 μg/mL Compound: 7	Antiviral activity against HIV-1 3B infected in human C8166 cells assessed as inhibition of syncytia formation after 3 days Antiviral activity against HIV-1 3B infected in human C8166 cells assessed as inhibition of syncytia formation after 3 days	[PMID: 23368966]
Hep 3B2	IC₅₀	>30 μM Compound: 13, Moracin M	Cytotoxicity against human Hep3B cells assessed as cell viability after 24 hrs by MTT assay Cytotoxicity against human Hep3B cells assessed as cell viability after 24 hrs by MTT assay	[PMID: 25461329]
Hep 3B2	IC₅₀	>30 μM Compound: 4	Inhibition of hypoxia-induced HIF1 activation in human Hep3B cells by pGL3-HRE-luciferase reporter gene assay Inhibition of hypoxia-induced HIF1 activation in human Hep3B cells by pGL3-HRE-luciferase reporter gene assay	[PMID: 19072214]
Hep 3B2	IC₅₀	>50 μM Compound: 4	Inhibition of hypoxia-induced HIF1alpha protein accumulation in human Hep3B cells treated for 30 mins measured after 12 hrs by Western blot analysis Inhibition of hypoxia-induced HIF1alpha protein accumulation in human Hep3B cells treated for 30 mins measured after 12 hrs by Western blot analysis	[PMID: 19072214]
Hep 3B2	IC₅₀	>50 μM Compound: 4	Inhibition of hypoxia-induced VEGF protein secretion in human Hep3B cells after 16 hrs by ELISA Inhibition of hypoxia-induced VEGF protein secretion in human Hep3B cells after 16 hrs by ELISA	[PMID: 19072214]
HT-29	IC₅₀	73 μM Compound: 11	Cytotoxicity against human HT-29 cells incubated for 24 hrs by MTT assay Cytotoxicity against human HT-29 cells incubated for 24 hrs by MTT assay	[PMID: 36463729]

In Vitro

Moracin M (30, 60 μM, 2h) inhibits the expression of IL-6 in A549 cells by inhibiting the activation of JNK/c-Jun. The IC₅₀ value of Moracin M for IL-6 is 8.1 μM^[1].
Moracin M inhibits the production of NO by inhibiting the expression of iNOS in alveolar macrophages (MH-S). The IC₅₀ value of Moracin M for NO is 65.7 μM^[2].
Moracin M (LPS 0.5 μg/mL, Moracin M 5-20 μM; 24h) can effectively inhibits the inflammatory response induced by lipopolysaccharide (LPS (HY-D1056)) in the intervertebral disc nucleus pulposus cell (NPCs) model, specifically by reducing the increase of inflammatory factors IL-1β, TNF-α and IL-6 ^[3].
Moracin M (LPS 0.5 μg/mL, Moracin M 5-20 μM; 24h) promots the autophagy and inhibits the production of inflammatory mediators in NPCs model by inhibiting the phosphorylation of PI3K and Akt ^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

RT-PCR^[2]

Cell Line:

A549 human lung epithelial cell line
Concentration:

30, 60 μM
Incubation Time:

2h
Result:

Inhibited the activation of c-Jun N-terminal kinase (JNK) and extracellular signal-regulated kinase (ERK)

Western Blot Analysis^[2]

Cell Line:

A549 human lung epithelial cell line
Concentration:

30, 60 μM
Incubation Time:
Result:

Clearly inhibited IL-6 expression in IL-1β-treated lung epithelial cells.
The expression of iNOS was significantly inhibited.

Western Blot Analysis^[2]

Cell Line:

A549 human lung epithelial cell line
Concentration:

30, 60 μM
Incubation Time:
Result:

Clearly inhibited IL-6 expression in IL-1β-treated lung epithelial cells.
The expression of iNOS was significantly inhibited.

Western Blot Analysis^[2]

Cell Line:

lipopolysaccharide (LPS)-treated nucleus pulposus cells (NPCs)
Concentration:

LPS (HY-D1056) 0.5 μg/mL , Moracin M 5-20 μM
Incubation Time:

24h
Result:

Effectively inhibited lipopolysaccharide (LPS) -induced inflammatory response, specifically by reducing the increase of inflammatory cytokines IL-1β, TNF-α and IL-6
Inhibited the phosphorylation of PI3K and Akt, promoted the autophagy of NPCs (autophagy) and inhibited the production of inflammatory mediators

In Vivo

Moracin M (20, 60 mg/kg, Oral gavage) reduces inflammatory response in LPS (HY-D1056) -induced acute lung injury (ALI) mouse models, decreases the total number of infiltrated cells in bronchoalveolar lavage (BAL) fluid, and significantly reduces the number of neutrophils, macrophages and dendritic cells^[2].
Moracin M (20, 60 mg/kg, Oral gavage) alleviates pathological changes in lung tissue in ALI mouse models, including reduces alveolar wall thickness, increases alveolar space, and reduces inflammatory cell infiltration in lung tissue^[2].
Moracin M (20, 60 mg/kg, Oral gavage) significantly inhibits NF-κB activation in the lungs in a mouse model of ALI^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

LPS-induced acute lung injury (ALI) mouse models^[2]
Dosage:

LPS (HY-D1056) 2 mg/kg; LPS 2 mg/kg+ Moracin M 20 mg/kg, 60 mg/kg; LPS 2 mg/kg+ Dexamethasone (HY-14648) 30 mg/kg. (n=13)
Administration:

Oral gavage
Result:

Reduced the inflammatory response in ALI mouse models and reduced the total number of cells infiltrated in bronchoalveolar lavage (BAL) fluid. The number of neutrophils, macrophages and dendritic cells was significantly decreased.
Reduced the thickness of alveolar wall, increasing alveolar space, and reduced the infiltration of inflammatory cells in lung tissue.

Chemical Information

CAS No. 56317-21-6
Appearance Solid
Molecular Weight 242.23
Formula C₁₄H₁₀O₄
Color Off-white to light yellow
SMILES

OC1=CC(C2=CC3=CC=C(O)C=C3O2)=CC(O)=C1
Structure Classification
- Phenols
- Polyphenols
Initial Source
- Endogenous metabolite
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (4)

All Cell Proliferation/Viability AssayWBRT-PCR

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Journal Impact Factor

J Agric Food Chem

Comprehensive Quantification of (Poly)phenols in Lotus japonicus with and without Arbuscular Mycorrhizal Symbiosis. [Abstract]

2025 Jun 4;73(22):14044-14057. PMID: 40418640
Environ Microbiol

Unravelling Wood Extractive Resistance in Phanerochaete chrysosporium Through Random Mutagenesis. [Abstract]

2025 Dec;27(12):e70205. PMID: 41342371
Arch Dermatol Res

Moracin M promotes hair regeneration through activation of the WNT/β-catenin pathway and angiogenesis. [Abstract]

2025 Jan 24;317(1):304. PMID: 39853610

Moracin M purchased from MedChemExpress. Usage Cited in: Arch Dermatol Res. 2025 Jan 24;317(1):304. [Abstract]

The cell viability of human Dermal Papilla Cells (hDPCs) was assessed following treatment with 100 ng/mL of IFN-γ, distinguishing between the anagen and catagen phases. Moracin M at 25 and 50 µM significantly increased cell proliferation.

Moracin M purchased from MedChemExpress. Usage Cited in: Arch Dermatol Res. 2025 Jan 24;317(1):304. [Abstract]

Western blots were performed on Wnt3a, GSK3β, and β-catenin in hDPCs after treatment with Moracin M (12.5, 25, 50 μM) with IFN-γ.

Moracin M purchased from MedChemExpress. Usage Cited in: Arch Dermatol Res. 2025 Jan 24;317(1):304. [Abstract]

The mRNA expression of VEGF gene in hDPCs treated with Moracin M (12.5, 25, 50 μM) with IFN-γ.
Res Sq

2024 Nov 19.

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (412.83 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

4.1283 mL

20.6415 mL

41.2831 mL

5 mM

0.8257 mL

4.1283 mL

8.2566 mL

10 mM

0.4128 mL

2.0642 mL

4.1283 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass

Concentration

Volume

Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 1.67 mg/mL (6.89 mM); Clear solution

This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 1.67 mg/mL (6.89 mM); Clear solution

This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 1.67 mg/mL (6.89 mM); Clear solution

This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage
mg/kg
Animal Weight (Per Animal)
g
Dosing Volume (Per Animal)
μL
Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (281 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Chen SK, et al. Moracin M from Morus alba L. is a natural phosphodiesterase-4 inhibitor. Bioorg Med Chem Lett. 2012 May 1;22(9):3261-4. [Content Brief]

[2]. Lee JH, et al. Moracin M inhibits airway inflammation by interrupting the JNK/c-Jun and NF-κB pathways in vitro and in vivo. Eur J Pharmacol. 2016;783:64-72. [Content Brief]

[3]. Guo F, et al. Moracin M inhibits lipopolysaccharide-induced inflammatory responses in nucleus pulposus cells via regulating PI3K/Akt/mTOR phosphorylation. Int Immunopharmacol. 2018 May;58:80-86. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	4.1283 mL	20.6415 mL	41.2831 mL	103.2077 mL
	5 mM	0.8257 mL	4.1283 mL	8.2566 mL	20.6415 mL
	10 mM	0.4128 mL	2.0642 mL	4.1283 mL	10.3208 mL
	15 mM	0.2752 mL	1.3761 mL	2.7522 mL	6.8805 mL
	20 mM	0.2064 mL	1.0321 mL	2.0642 mL	5.1604 mL
	25 mM	0.1651 mL	0.8257 mL	1.6513 mL	4.1283 mL
	30 mM	0.1376 mL	0.6881 mL	1.3761 mL	3.4403 mL
	40 mM	0.1032 mL	0.5160 mL	1.0321 mL	2.5802 mL
	50 mM	0.0826 mL	0.4128 mL	0.8257 mL	2.0642 mL
	60 mM	0.0688 mL	0.3440 mL	0.6881 mL	1.7201 mL
	80 mM	0.0516 mL	0.2580 mL	0.5160 mL	1.2901 mL
	100 mM	0.0413 mL	0.2064 mL	0.4128 mL	1.0321 mL

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Moracin M

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Publications Citing Use of MedChemExpress (MCE) Moracin M

All Phosphodiesterase (PDE) Isoforms

All Endogenous Metabolite Isoforms

All NF-κB Isoforms

Biological Activity

Chemical Information

Publications (4)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Moracin M Related Classifications

Cat. No.: HY-122942