AG490
Based on 165 publication(s) in Google Scholar
AG490 (Tyrphostin AG490) is a tyrosine kinase inhibitor that inhibits EGFR, Stat-3 and JAK2/3.
For research use only. We do not sell to patients.
- Purity: 99.86%
- CAS No.: 133550-30-8
- Formula: C17H14N2O3
- Molecular Weight:294.30
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Biological Activity
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Chemical Information
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Publications Citing Use of MedChemExpress (MCE) AG490
More- Cell Res. 2022 Jun;32(6):513-529. [Abstract]
- Nat Commun. 2023 May 31;14(1):3159. [Abstract]
- Chem Eng J. 2024 Mar 1, 483, 149235.
- J Exp Clin Cancer Res. 2024 Jun 14;43(1):164. [Abstract]
- J Exp Clin Cancer Res. 2019 Aug 22;38(1):370. [Abstract]
- J Nanobiotechnology. 2025 Aug 29;23(1):592. [Abstract]
- J Nanobiotechnology. 2025 Jul 14;23(1):509. [Abstract]
- Sci Adv. 2024 Mar 22;10(12):eadl0368. [Abstract]
- Int J Oral Sci. 2024 Jul 1;16(1):47. [Abstract]
- Redox Biol. 2024 Feb:69:102982. [Abstract]
- J Immunother Cancer. 2024 Oct 23;12(10):e010148. [Abstract]
- Int J Biol Sci. 2026 Jan 15;22(4):1834-1851. [Abstract]
- Int J Biol Sci. 2023 Apr 2;19(7):2021-2033. [Abstract]
- Cell Death Dis. 2019 Jun 13;10(6):465. [Abstract]
- Cell Death Dis. 2019 Apr 30;10(5):353. [Abstract]
- Cell Death Dis. 2018 Aug 29;9(9):867. [Abstract]
- J Cachexia Sarcopenia Muscle. 2024 Aug;15(4):1601-1615. [Abstract]
- Int J Biol Macromol. 2022 May 15:207:559-569. [Abstract]
- Acta Pharmacol Sin. 2024 Oct;45(10):2077-2091. [Abstract]
- Acta Pharmacol Sin. 2019 Jul;40(7):879-894. [Abstract]
- Phytomedicine. 2025 Oct 15:148:157414. [Abstract]
- Phytomedicine. 2024 Aug 15:134:155966. [Abstract]
- Phytomedicine. 2020 Apr;69:153194. [Abstract]
- Sci Total Environ. 2022 Jul 10;829:154437. [Abstract]
- Mater Des. 2026 Feb 3.
- Brain Behav Immun. 2019 Aug:80:711-724. [Abstract]
- J Transl Med. 2025 Jul 10;23(1):780. [Abstract]
- Biomed Pharmacother. 2022 May;149:112836. [Abstract]
- Biomed Pharmacother. 2020 Oct;130:110534. [Abstract]
- Redox Rep. 2025 Dec;30(1):2444755. [Abstract]
- Cell Mol Gastroenterol Hepatol. 2024;17(2):219-235. [Abstract]
- Stem Cell Res Ther. 2021 Oct 18;12(1):544. [Abstract]
- Oncogene. 2017 May 25;36(21):2946-2956. [Abstract]
- Cell Chem Biol. 2022 Jul 21;29(7):1113-1125.e6. [Abstract]
- Aging Cell. 2024 Aug 15:e14305. [Abstract]
- Cell Rep. 2023 Mar 20;42(3):112275. [Abstract]
- Cell Rep. 2022 Nov 8;41(6):111591. [Abstract]
- Mol Med. 2025 Apr 7;31(1):128. [Abstract]
- Mol Med. 2024 Feb 1;30(1):18. [Abstract]
- J Agric Food Chem. 2021 Jul 21;69(28):7898-7909. [Abstract]
- J Agric Food Chem. 2018 Nov 7;66(44):11757-11766. [Abstract]
- Ecotoxicol Environ Saf. 2025 Jun 27:302:118595. [Abstract]
- Ecotoxicol Environ Saf. 2025 Apr 17:296:118202. [Abstract]
- Cell Biol Toxicol. 2024 Dec 21;41(1):7. [Abstract]
- J Mol Cell Biol. 2023 Aug 3;15(3):mjad017. [Abstract]
- Food Biosci. April 2022, 101571.
- Int J Mol Med. 2020 Jul;46(1):191-200. [Abstract]
- Chin Med. 2026 Jan 28;21(1):56. [Abstract]
- Breast Cancer Res. 2025 Oct 23;27(1):184. [Abstract]
- Chem Biol Interact. 2025 Oct 8:419:111665. [Abstract]
- J Ethnopharmacol. 2025 Jan 7:119336. [Abstract]
- J Ethnopharmacol. 2024 Aug 10:330:118196. [Abstract]
- J Ethnopharmacol. 2022 Sep 15:295:115422. [Abstract]
- J Ethnopharmacol. 2021 Apr 24:270:113825. [Abstract]
- J Ethnopharmacol. 2019 Mar 25:232:62-72. [Abstract]
- Mech Ageing Dev. 2020 Oct;191:111347. [Abstract]
- CNS Neurosci Ther. 2025 Sep;31(9):e70609. [Abstract]
- Immunology. 2025 Aug;175(4):534-543. [Abstract]
- CNS Neurosci Ther. 2024 Mar;30(3):e14428. [Abstract]
- J Thromb Haemost. 2021 Aug;19(8):2029-2043. [Abstract]
- Int J Mol Sci. 2024 Jul 15;25(14):7755. [Abstract]
- Int J Mol Sci. 2022 Apr 12;23(8):4277. [Abstract]
- Front Pharmacol. 2021 Dec 14;12:746786. [Abstract]
- Int Immunopharmacol. 2024 Oct 18;143(Pt 2):113387. [Abstract]
- Int Immunopharmacol. 2024 Oct 18;143(Pt 2):113416. [Abstract]
- Int Immunopharmacol. 2024 Sep 24;142(Pt B):113221. [Abstract]
- Int Immunopharmacol. 2024 Aug 24:141:113012. [Abstract]
- Am J Physiol Cell Physiol. 2023 Oct 1;325(4):C1119-C1130. [Abstract]
- Eur J Pharmacol. 2022 Nov 15:935:175326. [Abstract]
- Int Immunopharmacol. 2022 Dec;113(Pt A):109340. [Abstract]
- Int Immunopharmacol. 2022 May 6;109:108819. [Abstract]
- Int Immunopharmacol. 2022 Aug:109:108840. [Abstract]
- Int Immunopharmacol. 2021 Jul:96:107639. [Abstract]
- Reprod Biol Endocrinol. 2018 May 31;16(1):55. [Abstract]
- Toxicology. 2023 Nov:499:153657. [Abstract]
- Adv Nanobiomed Res. 14 July 2022.
- Mol Neurobiol. 2026 Jan 23;63(1):390. [Abstract]
- Mol Neurobiol. 2025 Jun 4. [Abstract]
- Biol Proced Online. 2024 Aug 26;26(1):27. [Abstract]
- Exp Gerontol. 2021 Aug:151:111400. [Abstract]
- Front Cell Dev Biol. 2019 Nov 1;7:253. [Abstract]
- J Cell Mol Med. 2024 Oct;28(19):e18267. [Abstract]
- Poult Sci. 2024 Oct 19;103(12):104434. [Abstract]
- Biochim Biophys Acta Mol Basis Dis. 2024 Sep 5:167498. [Abstract]
- Exp Neurol. 2023 Oct:368:114474. [Abstract]
- Biochim Biophys Acta Mol Basis Dis. 2023 Apr;1869(4):166665. [Abstract]
- FEBS J. 2021 Jun;288(11):3530-3546. [Abstract]
- J Cell Mol Med. 2020 Dec;24(23):13763-13774. [Abstract]
- iScience. 2024 Feb 9;27(3):109160. [Abstract]
- J Inflamm Res. 2023 Mar 10:16:1027-1042. [Abstract]
- J Inflamm Res. 2021 Mar 5;14:719-735. [Abstract]
- Transl Oncol. 2018 Dec 26;12(3):485-492. [Abstract]
- J Cell Physiol. 2024 Feb;239(2):e31117. [Abstract]
- J Neurochem. 2017 Oct;143(1):87-99. [Abstract]
- Sci Rep. 2026 Mar 5. [Abstract]
- Sci Rep. 2026 Mar 21;16(1):14358. [Abstract]
- Biomedicines. 2022 Sep 22;10(10):2358. [Abstract]
- Aging (Albany NY). 2021 Mar 31;13(7):10517-10534. [Abstract]
- Sci Rep. 2020 Jul 9;10(1):11269. [Abstract]
- Aging (Albany NY). 2019 Oct 14;11(19):8120-8138. [Abstract]
- J Pharmacol Exp Ther. 2026 Apr;393(4):104306. [Abstract]
- J Virol. 2023 Apr 27;97(4):e0188922. [Abstract]
- Chem Res Toxicol. 2022 Mar 21;35(3):422-430. [Abstract]
- Cell Signal. 2025 May 17:111877. [Abstract]
- Cytokine. 2021 Feb:138:155377. [Abstract]
- Heliyon. 2024 Aug 6;10(15):e35665. [Abstract]
- Exp Cell Res. 2025 Sep 15;452(2):114753. [Abstract]
- J Bone Oncol. 2022 Nov 14:37:100461. [Abstract]
- Mol Med Rep. 2022 Mar;25(3):76. [Abstract]
- Mol Med Rep. 2019 Sep;20(3):2893-2901. [Abstract]
- Mol Med Rep. 2018 Jun;17(6):7595-7602. [Abstract]
- Mol Med Rep. 2017 Dec;16(6):9309-9316. [Abstract]
- Andrology. 2025 May;13(4):934-954. [Abstract]
- Andrology. 2024 May 22. [Abstract]
- J Immunol. 2019 May 15;202(10):3065-3075. [Abstract]
- World J Clin Oncol. 2025 Dec 24;16(12):112626. [Abstract]
- Hum Exp Toxicol. 2025 Jan-Dec:44:9603271251324582. [Abstract]
- J Cancer. 2024 Jul 9;15(14):4759-4776. [Abstract]
- Int J Cardiol. 2024 Jan 15:395:131426. [Abstract]
- J Pharm Pharmacol. 2023 Jan 31;75(1):87-97. [Abstract]
- Funct Integr Genomics. 2025 Sep 2;25(1):184. [Abstract]
- Cell Biol Int. 2021 Dec;45(12):2420-2428. [Abstract]
- Mol Immunol. 2024 Sep 20:175:55-62. [Abstract]
- Arch Biochem Biophys. 2022 Sep 30:727:109325. [Abstract]
- Burns. 2025 Dec;51(9):107671. [Abstract]
- Am J Cancer Res. 2023 Feb 15;13(2):589-601. [Abstract]
- Am J Cancer Res. 2020 Jan 1;10(1):224-236. [Abstract]
- BMC Pulm Med. 2025 Aug 14;25(1):389. [Abstract]
- J Cancer Res Clin Oncol. 2025 Jan 24;151(2):46. [Abstract]
- Neuroscience. 2023 Aug 21:526:314-325. [Abstract]
- Oncotargets Ther. 2021 May 28;14:3487-3501. [Abstract]
- Onco Targets Ther. 2019 Jul 5:12:5323-5334. [Abstract]
- Mol Cell Biol. 2026 Mar 19:1-18. [Abstract]
- Immun Inflamm Dis. 2025 Oct;13(10):e70287. [Abstract]
- Vet Microbiol. 2024 Aug 24:298:110235. [Abstract]
- Exp Eye Res. 2024 May 22:109939. [Abstract]
- Int J Immunopathol Pharmacol. 2024 Jan-Dec:38:3946320241249445. [Abstract]
- Cell Biochem Biophys. 2025 Jun;83(2):1785-1798. [Abstract]
- Tissue Cell. 2023 Jun:82:102082. [Abstract]
- Theriogenology. 2023 Jul 15:205:114-129. [Abstract]
- Reprod Sci. 2019 Jun;26(6):829-838. [Abstract]
- J Voice. 2025 Nov 29:S0892-1997(25)00490-4. [Abstract]
- Am J Physiol Regul Integr Comp Physiol. 2023 Jun 1;324(6):R747-R760. [Abstract]
- Insect Mol Biol. 2021 Feb;30(1):102-112. [Abstract]
- Biochem Biophys Res Commun. 2026 Apr 9:808:153453. [Abstract]
- Hum Immunol. 2024 Oct 1;85(6):111150. [Abstract]
- Biomol Biomed. 2024 Dec 11;25(1):249-258. [Abstract]
- Leuk Lymphoma. 2023 Jan;64(1):71-78. [Abstract]
- Thromb J. 2022 Apr 29;20(1):24. [Abstract]
- Folia Histochem Cytobiol. 2021;59(3):187-194. [Abstract]
- Biochem Biophys Rep. 2021 Sep 17:28:101133. [Abstract]
- Biochem Biophys Res Commun. 2020 Apr 2;524(2):516-522. [Abstract]
- Cardiovasc Diagn Ther. 2021 Apr;11(2):422-434. [Abstract]
- Biol Pharm Bull. 2024;47(9):1511-1524. [Abstract]
- Biochem Genet. 2025 Jun;63(3):2743-2759. [Abstract]
- Curr Med Sci. 2022 Oct;42(5):932-940. [Abstract]
- Braz J Med Biol Res. 2022 Jul 13;55:e12145. [Abstract]
- SSRN. 2025 Dec 26.
- Res Sq. 2024 Jun 17.
- SSRN. 2023 Nov 21.
- Research Square Print. 2023 Jan 5.
- Authorea. December 16, 2022.
- Research Square Preprint. 2021 Sep.
- Research Square Preprint. 2021 Apr.
- Research Square Preprint. 2020 Jun.
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IF
All EGFR Isoforms
More
Biological Activity
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EGFR |
Stat-3 |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A-431 | IC50 |
2 μM
Compound: 138, AG-490
|
Inhibition of EGFR in human A431 cells
Inhibition of EGFR in human A431 cells
|
[PMID: 1479375] |
| HeLa | IC50 |
>100 μM
Compound: AG-490
|
Cytotoxicity against human HeLa cells after 48 hrs by MTT assay
Cytotoxicity against human HeLa cells after 48 hrs by MTT assay
|
[PMID: 21185195] |
| HFF | IC50 |
5584 nM
Compound: AG-490
|
Inhibition of fetal calf serum-induced proliferation of human foreskin fibroblast assessed as [3H]thymidine incorporation after 4 days by scintillation counting
Inhibition of fetal calf serum-induced proliferation of human foreskin fibroblast assessed as [3H]thymidine incorporation after 4 days by scintillation counting
|
[PMID: 14593182] |
| HT-29 | IC50 |
78.523 μM
Compound: AG-490
|
Cytotoxicity against human HT-29 cells after 48 hrs by MTT assay
Cytotoxicity against human HT-29 cells after 48 hrs by MTT assay
|
[PMID: 21185195] |
| Sf9 | IC50 |
>10000 nM
Compound: AG-490
|
Inhibition of human recombinant GST-tagged JH1 domain (785 to1125) of JAK3 expressed in insect Sf9 cells by ELISA
Inhibition of human recombinant GST-tagged JH1 domain (785 to1125) of JAK3 expressed in insect Sf9 cells by ELISA
|
[PMID: 14593182] |
| T-cell | IC50 |
3750 nM
Compound: AG-490
|
Inhibition of IL2-induced proliferation of human T cells assessed as [3H]thymidine incorporation after 72 hrs by scintillation counting
Inhibition of IL2-induced proliferation of human T cells assessed as [3H]thymidine incorporation after 72 hrs by scintillation counting
|
[PMID: 14593182] |
| U-266 | IC50 |
>12.5 μM
Compound: AG-490
|
Antitumor activity against human U266 cells after 24 to 72 hrs by MTT assay
Antitumor activity against human U266 cells after 24 to 72 hrs by MTT assay
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[PMID: 22036213] |
AG490 inhibits the activation of Stat-3 by selectively blocking JAK2. AG490 is used to selectively inhibit JAK/Stat-3 activation. At a dose of 10 μM, Stat-3 phosphorylation is decreased by >95% and cell viability is maintained. AG490 at a dose of 10 μM results in >95% decrease in pStat-3 in EGF-stimulated A431 cells with no effect on Stat-3 mass[1]. AG-490 is a potent inhibitor of the JAK3/STAT, JAK3/AP-1, and JAK3/MAPK pathways and their cellular consequences. AG-490 abolishes IL-2-inducible [3H]thymidine incorporation in a dose-dependent manner, displaying an IC50 of 25 μM. AG-490 potently inhibits IL-2-mediated proliferation in T cells, results distinct from previous studies that showed this agent induced apoptosis in ALL cells while exerting apparently no effects on the growth of mitogen-stimulated normal T cells[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 133550-30-8
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Appearance Solid
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Molecular Weight 294.30
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Formula C17H14N2O3
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Color Light yellow to yellow
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SMILES
O=C(NCC1=CC=CC=C1)/C(C#N)=C/C2=CC=C(O)C(O)=C2
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Synonyms
Tyrphostin AG490; Tyrphostin B42
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (165)
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Journal Impact Factor
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Most Recent
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Cell Res
Derivation of totipotent-like stem cells with blastocyst-like structure forming potential. [Abstract]2022 Jun;32(6):513-529. PMID: 35508506 -
Nat Commun
An injectable liposome-anchored teriparatide incorporated gallic acid-grafted gelatin hydrogel for osteoarthritis treatment. [Abstract]2023 May 31;14(1):3159. PMID: 37258510 -
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J Exp Clin Cancer Res
ALKBH5 promotes non-small cell lung cancer progression and susceptibility to anti-PD-L1 therapy by modulating interactions between tumor and macrophages. [Abstract]2024 Jun 14;43(1):164. PMID: 38872221 -
J Exp Clin Cancer Res
Topoisomerase inhibitors promote cancer cell motility via ROS-mediated activation of JAK2-STAT1-CXCL1 pathway. [Abstract]2019 Aug 22;38(1):370. PMID: 31438997 -
J Nanobiotechnology
The bone marrow mesenchymal stem cells derived migrasomes induced by Titania nanotubes surface serve as chemotaxis effect for osteogenesis. [Abstract]2025 Aug 29;23(1):592. PMID: 40877924 -
J Nanobiotechnology
Engineered Panax notoginseng polysaccharide micelles inhibit macrophage polarization and delay the progression of rheumatoid arthritis via JAK2-STAT3 signaling pathway. [Abstract]2025 Jul 14;23(1):509. PMID: 40660236 -
Sci Adv
2024 Mar 22;10(12):eadl0368. PMID: 38507500 -
Int J Oral Sci
Stabilization of EREG via STT3B-mediated N-glycosylation is critical for PDL1 upregulation and immune evasion in head and neck squamous cell carcinoma. [Abstract]2024 Jul 1;16(1):47. PMID: 38945975 -
Redox Biol
IL-10 protects against OPC ferroptosis by regulating lipid reactive oxygen species levels post stroke. [Abstract]2024 Feb:69:102982. PMID: 38070317 -
J Immunother Cancer
Targeting CD93 on monocytes revitalizes antitumor immunity by enhancing the function and infiltration of CD8+ T cells. [Abstract]2024 Oct 23;12(10):e010148. PMID: 39448202 -
Int J Biol Sci
Abnormal mechanical load aggravates subchondral bone remodeling and uneven tibial plateau settlement in knee osteoarthritis via activation of osteoblast Piezo1-Ca²⁺-JAK2/STAT3 signaling. [Abstract]2026 Jan 15;22(4):1834-1851. PMID: 41694589 -
Int J Biol Sci
2023 Apr 2;19(7):2021-2033. PMID: 37151888 -
Cell Death Dis
Aquaporin 3 maintains the stemness of CD133+ hepatocellular carcinoma cells by activating STAT3. [Abstract]2019 Jun 13;10(6):465. PMID: 31197130
AG490 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2019 Jun 13;10(6):465. [Abstract]
pcDNA-AQP3 is used to upregulated AQP3 expression. We found AQP3 overexpression had no influence on STAT3 expression, but could promote STAT3 activation. AG-490 treatment could inhibit STAT3 activation and eliminate the influence of AQP3 overexpression.
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Cell Death Dis
IL-11 is essential in promoting osteolysis in breast cancer bone metastasis via RANKL-independent activation of osteoclastogenesis. [Abstract]2019 Apr 30;10(5):353. PMID: 31040267 -
Cell Death Dis
HGF-mediated crosstalk between cancer-associated fibroblasts and MET-unamplified gastric cancer cells activates coordinated tumorigenesis and metastasis. [Abstract]2018 Aug 29;9(9):867. PMID: 30158543
AG490 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2018 Aug 29;9(9):867. [Abstract]
AG490 decreases c-Met expression in MGC803 and AGS cells when they are co-cultured with CAFs.
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J Cachexia Sarcopenia Muscle
2024 Aug;15(4):1601-1615. PMID: 39031684 -
Int J Biol Macromol
Polyethylenimine-coated PLGA nanoparticles containing Angelica sinensis polysaccharide promote dendritic cells activation and associated molecular mechanisms. [Abstract]2022 May 15:207:559-569. PMID: 35288164 -
Acta Pharmacol Sin
ABBV-744 alleviates LPS-induced neuroinflammation via regulation of BATF2-IRF4-STAT1/3/5 axis. [Abstract]2024 Oct;45(10):2077-2091. PMID: 38862817 -
Acta Pharmacol Sin
Magnesium isoglycyrrhizinate ameliorates high fructose-induced liver fibrosis in rat by increasing miR-375-3p to suppress JAK2/STAT3 pathway and TGF-β1/Smad signaling. [Abstract]2019 Jul;40(7):879-894. PMID: 30568253 -
Phytomedicine
Baicalin ameliorates dextran sulfate solidum-induced colitis by modulating Th17-macrophage immune network via JAK2/STAT3/IL-17/NF-κB pathway. [Abstract]2025 Oct 15:148:157414. PMID: 41124707 -
Phytomedicine
Demethylzeylasteral ameliorates podocyte damage in murine lupus by inhibiting inflammation and enhancing autophagy. [Abstract]2024 Aug 15:134:155966. PMID: 39241387 -
Phytomedicine
Natural pseurotins and analogs thereof inhibit activation of B-cells and differentiation into the plasma cells. [Abstract]2020 Apr;69:153194. PMID: 32146299 -
Sci Total Environ
Maternal DBP exposure promotes synaptic formation in offspring by activating astrocytes via the AKT/NF-κB/IL-6/JAK2/STAT3 signaling pathway. [Abstract]2022 Jul 10;829:154437. PMID: 35278568 -
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Brain Behav Immun
CXCL13/CXCR5 signaling contributes to diabetes-induced tactile allodynia via activating pERK, pSTAT3, pAKT pathways and pro-inflammatory cytokines production in the spinal cord of male mice. [Abstract]2019 Aug:80:711-724. PMID: 31100371 -
J Transl Med
Siramesine induced cell death of glioblastoma through inactivating the STAT3-MGMT signaling pathway. [Abstract]2025 Jul 10;23(1):780. PMID: 40640878 -
Biomed Pharmacother
Formononetin protects against inflammation associated with cerebral ischemia-reperfusion injury in rats by targeting the JAK2/STAT3 signaling pathway. [Abstract]2022 May;149:112836. PMID: 35339827 -
Biomed Pharmacother
Fucoidan from Fucus vesiculosus attenuates doxorubicin-induced acute cardiotoxicity by regulating JAK2/STAT3-mediated apoptosis and autophagy. [Abstract]2020 Oct;130:110534. PMID: 32711244 -
Redox Rep
CEACAM5 exacerbates asthma by inducing ferroptosis and autophagy in airway epithelial cells through the JAK/STAT6-dependent pathway. [Abstract]2025 Dec;30(1):2444755. PMID: 39844719 -
Cell Mol Gastroenterol Hepatol
Oncostatin M induces IFITM1 expression to inhibit hepatitis B virus replication via JAK-STAT signaling. [Abstract]2024;17(2):219-235. PMID: 37879404 -
Stem Cell Res Ther
2021 Oct 18;12(1):544. PMID: 34663475 -
Oncogene
2017 May 25;36(21):2946-2956. PMID: 28092674
AG490 purchased from MedChemExpress. Usage Cited in: Oncogene. 2017 May 25;36(21):2946-2956. [Abstract]
(a) Immunoblotting analysis of pSTAT3, CITED4, and CCND1 in CL1-5 cells treated with AG490 (5 μM) for the indicated times. α-Tubulin is assessed as an internal control. (b) Q-PCR analysis of CITED4 in A549 cells treated with AG490 (5 μM) for the indicated times.
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Cell Chem Biol
Identifying enhancers of innate immune signaling as broad-spectrum antivirals active against emerging viruses. [Abstract]2022 Jul 21;29(7):1113-1125.e6. PMID: 35728599 -
Aging Cell
Upregulation of the Cav1.3 channel in inner hair cells by interleukin 6-dependent inflammaging contributes to age-related hearing loss. [Abstract]2024 Aug 15:e14305. PMID: 39148148 -
Cell Rep
Pharmacological boosting of cGAS activation sensitizes chemotherapy by enhancing antitumor immunity. [Abstract]2023 Mar 20;42(3):112275. PMID: 36943864 -
Cell Rep
The ApoA-I mimetic peptide 5A enhances remyelination by promoting clearance and degradation of myelin debris. [Abstract]2022 Nov 8;41(6):111591. PMID: 36351388 -
Mol Med
S-propargyl-cysteine attenuates temporomandibular joint osteoarthritis by regulating macrophage polarization via Inhibition of JAK/STAT signaling. [Abstract]2025 Apr 7;31(1):128. PMID: 40197110 -
Mol Med
Pretreatment with platelet-rich plasma protects against ischemia-reperfusion induced flap injury by deactivating the JAK/STAT pathway in mice. [Abstract]2024 Feb 1;30(1):18. PMID: 38302877 -
J Agric Food Chem
Promotion Effect of EGCG on the Raised Expression of IL-23 through the Signaling of STAT3-BATF2-c-JUN/ATF2. [Abstract]2021 Jul 21;69(28):7898-7909. PMID: 34227806 -
J Agric Food Chem
Glutamine Deficiency Promotes PCV2 Infection through Induction of Autophagy via Activation of ROS-Mediated JAK2/STAT3 Signaling Pathway. [Abstract]2018 Nov 7;66(44):11757-11766. PMID: 30343565
AG490 purchased from MedChemExpress. Usage Cited in: J Agric Food Chem. 2018 Nov 7;66(44):11757-11766. [Abstract]
The number of GFP-LC3 dots are reduced significantly after inhibiting STAT3 (AG-490) signaling.
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Ecotoxicol Environ Saf
Exposure to BDE-209 triggers necroptosis by activating the JAK2/STAT3 signaling pathway, thereby exacerbating neuroinflammation. [Abstract]2025 Jun 27:302:118595. PMID: 40580630 -
Ecotoxicol Environ Saf
The mitochondrial dysfunction regulated by JAK2/STAT3 pathway leads to the necroptosis in the renal cells under patulin exposure. [Abstract]2025 Apr 17:296:118202. PMID: 40249973 -
Cell Biol Toxicol
2024 Dec 21;41(1):7. PMID: 39707117 -
J Mol Cell Biol
2023 Aug 3;15(3):mjad017. PMID: 36921991 -
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Int J Mol Med
The JAK2/STAT3 pathway inhibitor, AG490, suppresses the abnormal behavior of keloid fibroblasts in vitro. [Abstract]2020 Jul;46(1):191-200. PMID: 32377718 -
Chin Med
Electroacupuncture ameliorates ulcerative colitis by suppressing ferroptosis via the JAK2/STAT3 signaling pathway. [Abstract]2026 Jan 28;21(1):56. PMID: 41606666 -
Breast Cancer Res
Modulating phosphodiesterase-5 activity to suppress the immunosuppressive mechanisms of myeloid-derived suppressor cells in breast cancer. [Abstract]2025 Oct 23;27(1):184. PMID: 41131545 -
Chem Biol Interact
Decabromodiphenyl ether (BDE-209) induces learning and memory impairment via JAK2/STAT3/NLRP3 axis-mediated pyroptosis and neuroinflammation. [Abstract]2025 Oct 8:419:111665. PMID: 40675405 -
J Ethnopharmacol
Chinese herbal formula Regan Saibisitan alleviates airway inflammation of chronic bronchitis via inhibiting the JAK2/STAT3 pathway. [Abstract]2025 Jan 7:119336. PMID: 39788165 -
J Ethnopharmacol
Rosmarinic acid alleviates toosendanin-induced liver injury through restoration of autophagic flux and lysosomal function by activating JAK2/STAT3/CTSC pathway. [Abstract]2024 Aug 10:330:118196. PMID: 38631488 -
J Ethnopharmacol
Th17/Treg imbalance mediates hepatic intolerance to exogenous lipopolysaccharide and exacerbates liver injury in triptolide induced excessive immune response. [Abstract]2022 Sep 15:295:115422. PMID: 35654348 -
J Ethnopharmacol
Modified Simiaowan prevents articular cartilage injury in experimental gouty arthritis by negative regulation of STAT3 pathway. [Abstract]2021 Apr 24:270:113825. PMID: 33460754 -
J Ethnopharmacol
Rheum palmatum extract exerts anti-hepatocellular carcinoma effects by inhibiting signal transducer and activator of transcription 3 signaling. [Abstract]2019 Mar 25:232:62-72. PMID: 30553869
AG490 purchased from MedChemExpress. Usage Cited in: J Ethnopharmacol. 2019 Mar 25:232:62-72. [Abstract]
After incubation with AG490 (50 μM), RP (40 μg/mL) or both for 24 h, total protein from SMMC-7721s are analyzed by Western blot.
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Mech Ageing Dev
AMPK alleviates oxidative stress‑induced premature senescence via inhibition of NF-κB/STAT3 axis-mediated positive feedback loop. [Abstract]2020 Oct;191:111347. PMID: 32882228 -
CNS Neurosci Ther
Network Pharmacology-Based and Experimental Validation Elucidate the Target Mechanism of Vinorine in Ameliorating Secondary Brain Injury After Intracerebral Hemorrhage. [Abstract]2025 Sep;31(9):e70609. PMID: 40994248 -
Immunology
Endogenous Hydrogen Sulphide Promotes the Ex Vivo Expansion of Haematopoietic Stem Cells by Regulating the Activation of the JAK2/STAT3 Pathway. [Abstract]2025 Aug;175(4):534-543. PMID: 40405487 -
CNS Neurosci Ther
Hypoxic-preconditioned mesenchymal stem cell-derived small extracellular vesicles promote the recovery of spinal cord injury by affecting the phenotype of astrocytes through the miR-21/JAK2/STAT3 pathway. [Abstract]2024 Mar;30(3):e14428. PMID: 37641874 -
J Thromb Haemost
Photobiomodulation therapy for thrombocytopenia by upregulating thrombopoietin expression via the ROS-dependent Src/ERK/STAT3 signaling pathway. [Abstract]2021 Aug;19(8):2029-2043. PMID: 33501731 -
Int J Mol Sci
Quercetin Attenuates KLF4-Mediated Phenotypic Switch of VSMCs to Macrophage-like Cells in Atherosclerosis: A Critical Role for the JAK2/STAT3 Pathway. [Abstract]2024 Jul 15;25(14):7755. PMID: 39062998 -
Int J Mol Sci
2022 Apr 12;23(8):4277. PMID: 35457095 -
Front Pharmacol
Yi Shen Juan Bi Pill Regulates the Bone Immune Microenvironment via the JAK2/STAT3 Signaling Pathway in Vitro. [Abstract]2021 Dec 14;12:746786. PMID: 34970139 -
Int Immunopharmacol
Tumor-associated macrophages induce epithelial-mesenchymal transition and promote lung metastasis in breast cancer by activating the IL-6/STAT3/TGM2 axis. [Abstract]2024 Oct 18;143(Pt 2):113387. PMID: 39426226 -
Int Immunopharmacol
NLRP3 regulates ferroptosis via the JAK2/STAT3 pathway in asthma inflammation: Insights from in vivo and in vitro studies. [Abstract]2024 Oct 18;143(Pt 2):113416. PMID: 39426227 -
Int Immunopharmacol
Unveiling the immunoregulatory role of interferon-induced transmembrane protein 2 through the JAK/STAT3/PDL1 pathway in gastric cancer. [Abstract]2024 Sep 24;142(Pt B):113221. PMID: 39321709 -
Int Immunopharmacol
Gastrodin against oxidative stress-inflammation crosstalk via inhibiting mtDNA/TLR9 and JAK2/STAT3 signaling to ameliorate ischemic stroke injury. [Abstract]2024 Aug 24:141:113012. PMID: 39182268 -
Am J Physiol Cell Physiol
α-ketoglutaric acid ameliorates intervertebral disc degeneration by blocking the IL-6/JAK2/STAT3 pathway. [Abstract]2023 Oct 1;325(4):C1119-C1130. PMID: 37661920 -
Eur J Pharmacol
Galangin induces the osteogenic differentiation of human amniotic mesenchymal stem cells via the JAK2/STAT3 signaling pathway. [Abstract]2022 Nov 15:935:175326. PMID: 36257381 -
Int Immunopharmacol
Blockade of JAK2 retards cartilage degeneration and IL-6-induced pain amplification in osteoarthritis. [Abstract]2022 Dec;113(Pt A):109340. PMID: 36330910 -
Int Immunopharmacol
Andrographolide attenuates Mycoplasma gallisepticum-induced inflammation and apoptosis by the JAK/PI3K/AKT signal pathway in the chicken lungs and primary alveolar type II epithelial cells. [Abstract]2022 May 6;109:108819. PMID: 35533556 -
Int Immunopharmacol
2022 Aug:109:108840. PMID: 35567856 -
Int Immunopharmacol
Piceatannol protects against sepsis-induced myocardial dysfunction via direct inhibition of JAK2. [Abstract]2021 Jul:96:107639. PMID: 34162128 -
Reprod Biol Endocrinol
Exogenous leptin affects sperm parameters and impairs blood testis barrier integrity in adult male mice. [Abstract]2018 May 31;16(1):55. PMID: 29855380
AG490 purchased from MedChemExpress. Usage Cited in: Reprod Biol Endocrinol. 2018 May 31;16(1):55. [Abstract]
Western blot analysis of claudin 5, occludin and ZO-1 in TM4 cells, cells are treated with 100 nM Leptin or pre-treated with different inhibitors following a 100 nM Leptin treatment.
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Toxicology
Recombinant Klotho alleviates vancomycin-induced acute kidney injury by upregulating anti-oxidative capacity via JAK2/STAT3/GPx3 axis. [Abstract]2023 Nov:499:153657. PMID: 37884167 -
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Mol Neurobiol
Silencing of Ifi27l2a Attenuates Inflammation After Spinal Cord Injury by Regulating Microglial Polarization via JAK2/STAT3 Signaling. [Abstract]2026 Jan 23;63(1):390. PMID: 41575677 -
Mol Neurobiol
RNF213 Knockdown and Inflammation Promote Angiogenesis and Phenotype Switching by JAK2/STAT3 Pathway in Moyamoya Disease. [Abstract]2025 Jun 4. PMID: 40467938 -
Biol Proced Online
Effects and Mechanisms of the Xianhecao-Huanglian Drug Pair on Autophagy-Mediated Intervention in Acute Inflammatory Bowel Disease via the JAK2/STAT3 Pathway. [Abstract]2024 Aug 26;26(1):27. PMID: 39187810 -
Exp Gerontol
Shenqi Yizhi Granule attenuates Aβ1-42 induced cognitive dysfunction via inhibiting JAK2/STAT3 activated astrocyte reactivity. [Abstract]2021 Aug:151:111400. PMID: 33974937 -
Front Cell Dev Biol
2019 Nov 1;7:253. PMID: 31737624
AG490 purchased from MedChemExpress. Usage Cited in: Front Cell Dev Biol. 2019 Nov 1;7:253. [Abstract]
STAT3 regulates miR-384 transcription. CD4C naïve T cells are cultured with IL-6, siRNA, or AG490, and then p-STAT3 and STAT3 levels are tested by Western blot.
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J Cell Mol Med
LTF ameliorates cartilage endplate degeneration by suppressing calcification, senescence and matrix degradation through the JAK2/STAT3 pathway. [Abstract]2024 Oct;28(19):e18267. PMID: 39392081 -
Poult Sci
Arbutin alleviates Mycoplasma gallinarum-induced damage caused by pulmonary fibrosis via the JAK2/STAT3 pathway. [Abstract]2024 Oct 19;103(12):104434. PMID: 39467406 -
Biochim Biophys Acta Mol Basis Dis
S100A4 mediates the accumulation and functions of myeloid-derived suppressor cells via GP130/JAK2/STAT3 signaling in acute myeloid leukemia. [Abstract]2024 Sep 5:167498. PMID: 39243827 -
Exp Neurol
Targeting the JAK2-STAT3 pathway to inhibit cGAS-STING activation improves neuronal senescence after ischemic stroke. [Abstract]2023 Oct:368:114474. PMID: 37419174 -
Biochim Biophys Acta Mol Basis Dis
FGFR2 upregulates PAI-1 via JAK2/STAT3 signaling to induce M2 polarization of macrophages in colorectal cancer. [Abstract]2023 Apr;1869(4):166665. PMID: 36781088 -
FEBS J
Alveolar epithelial cell-derived Sonic hedgehog promotes pulmonary fibrosis through OPN-dependent alternative macrophage activation. [Abstract]2021 Jun;288(11):3530-3546. PMID: 33314622 -
J Cell Mol Med
Phosphorylated STAT3 suppresses microRNA-19b/1281 to aggravate lung injury in mice with type 2 diabetes mellitus-associated pulmonary tuberculosis. [Abstract]2020 Dec;24(23):13763-13774. PMID: 33089914 -
iScience
PBRM1 presents a potential ctDNA marker to monitor response to neoadjuvant chemotherapy in cervical cancer. [Abstract]2024 Feb 9;27(3):109160. PMID: 38414861 -
J Inflamm Res
Pretreatment with Eupatilin Attenuates Inflammation and Coagulation in Sepsis by Suppressing JAK2/STAT3 Signaling Pathway. [Abstract]2023 Mar 10:16:1027-1042. PMID: 36926276 -
J Inflamm Res
KCa3.1 Inhibition of Macrophages Suppresses Inflammatory Response Leading to Endothelial Damage in a Cell Model of Kawasaki Disease. [Abstract]2021 Mar 5;14:719-735. PMID: 33727847 -
Transl Oncol
Overexpression of Indoleamine 2,3-Dioxygenase 1 Promotes Epithelial-Mesenchymal Transition by Activation of the IL-6/STAT3/PD-L1 Pathway in Bladder Cancer. [Abstract]2018 Dec 26;12(3):485-492. PMID: 30594037
AG490 purchased from MedChemExpress. Usage Cited in: Transl Oncol. 2018 Dec 26;12(3):485-492. [Abstract]
AG490 blocks the IL6/STAT3/PD-L1 signaling pathway and is more pronounced after knockdown of IDO1. T24 and UMUC3 cells after IDO1 knockdown are treated with STAT3 specific inhibitor AG490. The protein levels of STAT3, P-STAT3, and PD-L1 are measured by Western blot analysis.
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J Cell Physiol
Leptin excites basolateral amygdala principal neurons and reduces food intake by LepRb-JAK2-PI3K-dependent depression of GIRK channels. [Abstract]2024 Feb;239(2):e31117. PMID: 37683049 -
J Neurochem
Salvianolic acids enhance cerebral angiogenesis and neurological recovery by activating JAK2/STAT3 signaling pathway after ischemic stroke in mice. [Abstract]2017 Oct;143(1):87-99. PMID: 28771727 -
Sci Rep
2026 Mar 5. PMID: 41786829 -
Sci Rep
Targeting STAT3 pathway attenuates macrophages inflammation and cardiovascular injury in a model of Kawasaki disease. [Abstract]2026 Mar 21;16(1):14358. PMID: 41865139 -
Biomedicines
Alpha7 Nicotinic Acetylcholine Receptor Antagonists Prevent Meningitic Escherichia coli-Induced Blood-Brain Barrier Disruptions by Targeting the CISH/JAK2/STAT5b Axis. [Abstract]2022 Sep 22;10(10):2358. PMID: 36289622 -
Aging (Albany NY)
Bone marrow stem cells secretome accelerates simulated birth trauma-induced stress urinary incontinence recovery in rats. [Abstract]2021 Mar 31;13(7):10517-10534. PMID: 33793419 -
Sci Rep
Tensile force-induced PDGF-BB/PDGFRβ signals in periodontal ligament fibroblasts activate JAK2/STAT3 for orthodontic tooth movement. [Abstract]2020 Jul 9;10(1):11269. PMID: 32647179 -
Aging (Albany NY)
The interaction between STAT3 and nAChRα1 interferes with nicotine-induced atherosclerosis via Akt/mTOR signaling cascade. [Abstract]2019 Oct 14;11(19):8120-8138. PMID: 31612866 -
J Pharmacol Exp Ther
Targeting cholangiocyte sphingosine-1-phosphate (S1P) receptor 1 signaling alleviates cholestatic liver injury: Mechanistic insight into S1P/phosphorylated signal transducer and activator of transcription 3 axis. [Abstract]2026 Apr;393(4):104306. PMID: 41818851 -
J Virol
African Swine Fever Virus Envelope Glycoprotein CD2v Interacts with Host CSF2RA to Regulate the JAK2-STAT3 Pathway and Inhibit Apoptosis to Facilitate Virus Replication. [Abstract]2023 Apr 27;97(4):e0188922. PMID: 37022174 -
Chem Res Toxicol
P38 MAPK, NF-κB, and JAK-STAT3 Signaling Pathways Involved in Capecitabine-Induced Hand-Foot Syndrome via Interleukin 6 or Interleukin 8 Abnormal Expression. [Abstract]2022 Mar 21;35(3):422-430. PMID: 35147423 -
Cell Signal
2025 May 17:111877. PMID: 40389045 -
Cytokine
ITIH4, as an inflammation biomarker, mainly increases in bacterial bloodstream infection. [Abstract]2021 Feb:138:155377. PMID: 33348064 -
Heliyon
Leptin protects chondrocytes by inhibiting autophagy via phosphoinositide 3 kinase/protein kinase B/mammalian target of rapamycin signaling pathway. [Abstract]2024 Aug 6;10(15):e35665. PMID: 39170379 -
Exp Cell Res
TFAP4/DLGAP5 promotes tumor progression and macrophage M2 polarization in prostate cancer by activating the JAK2/STAT3 signaling. [Abstract]2025 Sep 15;452(2):114753. PMID: 40962169 -
J Bone Oncol
TIAM2 promotes proliferation and invasion of osteosarcoma cells by activating the JAK2/STAT3 signaling pathway. [Abstract]2022 Nov 14:37:100461. PMID: 36419799 -
Mol Med Rep
Role of TGM2 in T‑cell lymphoblastic lymphoma via regulation of IL‑6/JAK/STAT3 signalling. [Abstract]2022 Mar;25(3):76. PMID: 35014680 -
Mol Med Rep
Formononetin ameliorates high glucose‑induced endothelial dysfunction by inhibiting the JAK/STAT signaling pathway. [Abstract]2019 Sep;20(3):2893-2901. PMID: 31524234 -
Mol Med Rep
Crocin prevents platelet‑derived growth factor BB‑induced vascular smooth muscle cells proliferation and phenotypic switch. [Abstract]2018 Jun;17(6):7595-7602. PMID: 29620234
AG490 purchased from MedChemExpress. Usage Cited in: Mol Med Rep. 2018 Jun;17(6):7595-7602. [Abstract]
Representative images show the expression levels of α SMA, calponin and osteopontin (OPN) protein.
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Mol Med Rep
Promoting effects of IL‑23 on myocardial ischemia and reperfusion are associated with increased expression of IL‑17A and upregulation of the JAK2‑STAT3 signaling pathway. [Abstract]2017 Dec;16(6):9309-9316. PMID: 29039526
AG490 purchased from MedChemExpress. Usage Cited in: Mol Med Rep. 2017 Dec;16(6):9309-9316. [Abstract]
Ad IL 23 intensifies the high expression levels of IL 17A, TNF α and IL 6 induced by myocardial I/R, which is inhibited by AG490. Levels of IL 17A in myocardial tissues from infarct and risk regions (n=6). The addition of AG490 significantly inhibits the effect of Ad IL 23.
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Andrology
2025 May;13(4):934-954. PMID: 39253755 -
Andrology
Cellular mechanism underlying leptin-induced anion secretion of rat epididymal epithelial cells. [Abstract]2024 May 22. PMID: 38778669 -
J Immunol
FGFR2 Promotes Expression of PD-L1 in Colorectal Cancer via the JAK/STAT3 Signaling Pathway. [Abstract]2019 May 15;202(10):3065-3075. PMID: 30979816 -
World J Clin Oncol
Yin Yang 1 activates JAK-STAT3-mediated epithelial-mesenchymal transition in Helicobacter pylori-induced gastric cancer progression. [Abstract]2025 Dec 24;16(12):112626. PMID: 41480166 -
Hum Exp Toxicol
Rhynchophylline promotes microglia phenotypic transformation and repair of cerebral ischaemic injury through the JAK2/STAT3 pathway. [Abstract]2025 Jan-Dec:44:9603271251324582. PMID: 40014666 -
J Cancer
TLE4 downregulation identified by WGCNA and machine learning algorithm promotes papillary thyroid carcinoma progression via activating JAK/STAT pathway. [Abstract]2024 Jul 9;15(14):4759-4776. PMID: 39006072 -
Int J Cardiol
Loganin protects against myocardial ischemia-reperfusion injury by modulating oxidative stress and cellular apoptosis via activation of JAK2/STAT3 signaling. [Abstract]2024 Jan 15:395:131426. PMID: 37813285 -
J Pharm Pharmacol
Antiovarian cancer mechanism of esculetin: inducing G0/G1 arrest and apoptosis via JAK2/STAT3 signalling pathway. [Abstract]2023 Jan 31;75(1):87-97. PMID: 36332079 -
Funct Integr Genomics
CLDN4 promotes ferroptosis and inflammation involving JAK2/STAT3 pathway in acute pancreatitis. [Abstract]2025 Sep 2;25(1):184. PMID: 40892111 -
Cell Biol Int
The effect and mechanism of erianin on the reversal of oxaliplatin resistance in human colon cancer cells. [Abstract]2021 Dec;45(12):2420-2428. PMID: 34351659 -
Mol Immunol
IL-17A activates JAK/STAT signaling to affect drug metabolizing enzymes and transporters in HepaRG cells. [Abstract]2024 Sep 20:175:55-62. PMID: 39305848 -
Arch Biochem Biophys
Oxalate induces the ossification of RTECs by activating the JAK2/STAT3 signaling pathway and participates in the formation of kidney stones. [Abstract]2022 Sep 30:727:109325. PMID: 35749806 -
Burns
Baicalin promotes the survival of flaps by downregulating the JAK2-STAT3 signaling pathway. [Abstract]2025 Dec;51(9):107671. PMID: 40939303 -
Am J Cancer Res
Oral squamous cell carcinoma cell-derived GM-CSF regulates PD-L1 expression in tumor-associated macrophages through the JAK2/STAT3 signaling pathway. [Abstract]2023 Feb 15;13(2):589-601. PMID: 36895967 -
Am J Cancer Res
2020 Jan 1;10(1):224-236. PMID: 32064163 -
BMC Pulm Med
Transcriptomics reveals the underlying mechanism of Prostaglandin E1 in improving severe pneumonia. [Abstract]2025 Aug 14;25(1):389. PMID: 40804382 -
J Cancer Res Clin Oncol
2025 Jan 24;151(2):46. PMID: 39856473 -
Neuroscience
miR-146a-5p Modulates Adult Hippocampal Neurogenesis Deficits Through Klf4/p-Stat3 Signaling in APP/PS1 Mice. [Abstract]2023 Aug 21:526:314-325. PMID: 37321367 -
Oncotargets Ther
Acetone Extract of Cornus officinalis Leaves Exerts Anti-Melanoma Effects via Inhibiting STAT3 Signaling. [Abstract]2021 May 28;14:3487-3501. PMID: 34093025 -
Onco Targets Ther
TGFβ1 is essential for MSCs-CAFs differentiation and promotes HCT116 cells migration and invasion via JAK/STAT3 signaling. [Abstract]2019 Jul 5:12:5323-5334. PMID: 31308702 -
Mol Cell Biol
Paeonol Ameliorates Gastric Mucosal Enterosis and Dysplasia Associated with Chronic Atrophic Gastritis by Modulating the JAK2/STAT3 Pathway. [Abstract]2026 Mar 19:1-18. PMID: 41852098 -
Immun Inflamm Dis
Activated Peripheral CD8 + T Lymphocytes Inhibit the Proliferation of Hippocampal Neural Stem Cells via the IFN-γ/JAK/STAT Signaling Pathway. [Abstract]2025 Oct;13(10):e70287. PMID: 41146571 -
Vet Microbiol
Andrographolide inhibits porcine epidemic diarrhea virus by inhibiting the JAK2-STAT3 pathway and promoting apoptosis. [Abstract]2024 Aug 24:298:110235. PMID: 39213728 -
Exp Eye Res
Silibinin attenuates TGF-β2-induced fibrogenic changes in human trabecular meshwork cells by targeting JAK2/STAT3 and PI3K/AKT signaling pathways. [Abstract]2024 May 22:109939. PMID: 38789021 -
Int J Immunopathol Pharmacol
Low-dose metformin suppresses hepatocellular carcinoma metastasis via the AMPK/JNK/IL-8 pathway. [Abstract]2024 Jan-Dec:38:3946320241249445. PMID: 38679570 -
Cell Biochem Biophys
Sirt4 Overexpression Modulates the JAK2/STAT3 and PI3K/AKT/mTOR Axes to Alleviate Sepsis-Induced Acute Lung Injury. [Abstract]2025 Jun;83(2):1785-1798. PMID: 39400781 -
Tissue Cell
OTX1 silencing suppresses ovarian cancer progression through inhibiting the JAK/STAT signaling. [Abstract]2023 Jun:82:102082. PMID: 37027968 -
Theriogenology
MEL regulates miR-21 and let-7b through the STAT3 cascade in the follicular granulosa cells of Tibetan sheep. [Abstract]2023 Jul 15:205:114-129. PMID: 37120893 -
Reprod Sci
2019 Jun;26(6):829-838. PMID: 30526368
AG490 purchased from MedChemExpress. Usage Cited in: Reprod Sci. 2019 Jun;26(6):829-838. [Abstract]
The protein expression levels of p-JAK2, JAK2, p-STAT3, and STAT3 are detected by Western blot with the treatment of ,MSA, AG490 or MSA+AG490.
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J Voice
Deoxycholic Acid Drives ATP4A-Dependent Growth and Migration of Vocal Fold Leukoplakia Epithelial Cells via TGR5/JAK2/STAT3 Signaling. [Abstract]2025 Nov 29:S0892-1997(25)00490-4. PMID: 41320587 -
Am J Physiol Regul Integr Comp Physiol
Remote limb ischemic postconditioning attenuates myocardial dysfunction induced by testicular torsion/detorsion in rats. [Abstract]2023 Jun 1;324(6):R747-R760. PMID: 37036302 -
Insect Mol Biol
Target antifungal peptides of immune signalling pathways in silkworm, Bombyx mori, against Beauveria bassiana. [Abstract]2021 Feb;30(1):102-112. PMID: 33150694 -
Biochem Biophys Res Commun
Macrophage erythropoietin receptor signaling drives macrophage-myofibroblast transition via JAK2/STAT3 and TGF-β1/Smad3 pathways to exacerbate pulmonary fibrosis in mice. [Abstract]2026 Apr 9:808:153453. PMID: 41719615 -
Hum Immunol
Mechanism of LMNB1 activating GPR84 through JAK-STAT pathway to mediate M2 macrophage polarization in lung cancer. [Abstract]2024 Oct 1;85(6):111150. PMID: 39357468 -
Biomol Biomed
Botulinum toxin type A inhibits the formation of hypertrophic scar through the JAK2/STAT3 pathway. [Abstract]2024 Dec 11;25(1):249-258. PMID: 39132968 -
Leuk Lymphoma
IFN-γ enhances CLL cell resistance to ABT-199 by regulating MCL-1 and BCL-2 expression via the JAK-STAT3 signaling pathway. [Abstract]2023 Jan;64(1):71-78. PMID: 36222521 -
Thromb J
Complement C5a induces the generation of neutrophil extracellular traps by inhibiting mitochondrial STAT3 to promote the development of arterial thrombosis. [Abstract]2022 Apr 29;20(1):24. PMID: 35488279 -
Folia Histochem Cytobiol
SDF-1/CXCR4 axis promotes osteogenic differentiation of BMSCs through the JAK2/STAT3 pathway. [Abstract]2021;59(3):187-194. PMID: 34580847 -
Biochem Biophys Rep
Blockade of JAK2 signaling produces immunomodulatory effect to preserve pancreatic homeostasis in severe acute pancreatitis. [Abstract]2021 Sep 17:28:101133. PMID: 34584986 -
Biochem Biophys Res Commun
RAI3 knockdown enhances osteogenic differentiation of bone marrow mesenchymal stem cells via STAT3 signaling pathway. [Abstract]2020 Apr 2;524(2):516-522. PMID: 32014253 -
Cardiovasc Diagn Ther
The interplay between HIF-1α and long noncoding GAS5 regulates the JAK1/STAT3 signalling pathway in hypoxia-induced injury in myocardial cells. [Abstract]2021 Apr;11(2):422-434. PMID: 33968620 -
Biol Pharm Bull
Siweixizangmaoru Decoction Ameliorated Type II Collagen-Induced Arthritis in Rats via Regulating JAK2-STAT3 and NF-κB Signaling Pathway. [Abstract]2024;47(9):1511-1524. PMID: 39284735 -
Biochem Genet
Myricetin Restores Autophagy to Attenuate Lumbar Intervertebral Disk Degeneration Via Negative Regulation of the JAK2/STAT3 Pathway. [Abstract]2025 Jun;63(3):2743-2759. PMID: 38842745 -
Curr Med Sci
2022 Oct;42(5):932-940. PMID: 36245025 -
Braz J Med Biol Res
Dexmedetomidine pretreatment alleviates cerebral ischemia/reperfusion injury by inhibiting neuroinflammation through the JAK2/STAT3 pathway. [Abstract]2022 Jul 13;55:e12145. PMID: 35858000 -
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Solvent & Solubility
DMSO : ≥ 50 mg/mL (169.89 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (7.07 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.08 mg/mL (7.07 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocol
A colorimetric cell proliferation assay is performed using the CellTiter 96 kit. Briefly, A431 cells are plated in 96-well plates (2000 cells/well) and cultured in DMEM/HAM's F-12 supplemented with 10% FCS for 24 h. Cells are incubated in serum-free media for 24 h. EGF (10 ng/mL) is added to all wells. Tyrphostin AG1478 (0.25 mM) and AG490 (10 mM) are added alone or in combination and the culture is incubated for the appropriate time. Medium is aspirated and CellTiter 96 Aqueous One Solution Reagent (20 μL) is added to each well. The plates are incubated at 37°C for up to 1 h and absorbance recorded at 490 nm using a 96-well plate reader. Data are derived from at least three independent experiments (in triplicate) for the both single agents and combination studies. IC50 values for Tyrphostin AG1478 (EGFR inhibitor) and AG490 (JAK/STAT inhibitor) are determined. The growth inhibitory effects of the combination are quantified using the Calucsyn software program[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Mice[3]
Female NOD/LtJ, NOD.Scid, and BALB/c mice are used. One vial of compound containing 5 mg of AG490 is injected into5 mice (1 mg/mouse) via the i.p route. The control groups are receive the same volume of the vehicle under the same regimens and conditions.
Rats[4]
A total of 28 Male Sprague-Dawley rats (250-300 g) are used. The experiments are performed in rats 48 h after ʎ-carrageenan injection. A total of 4 groups (n=6) of rats are randomly included in the dose-response study. Group 1 is the vehicle control, which receive 100 µL i.pl. injection of 3.5% DMSO in saline. Groups 2-4 are injected with 3 different doses of AG490 (1, 5 or 10 µg). To study the effects of naloxone on AG490-induced antinociception, an additional group of rats (group 5; n=4) is observed. Group 5 is co-administered with AG490 (10 µg) and Naloxone (10 µg). The drugs are administered i.pl. in a volume of 100 µl. As reported earlier, the in vivo pharmacological effects of AG490 are observed 4 h after treatment. Thus, the behavioral tests are performed before (baseline assessment) and 4 h after treatment. First, the rats are subjected to the thermal hyperalgesia test; 10 min later, the paw pressure test is performed on the same set of rats. All the experiments are performed between 8:00 a.m. and 2:00 p.m. to reduce the confounding influence of diurnal variations, and all the procedures are performed in a blinded fashion.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (279 KB)
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SDS (623 KB)
- English - EN (623 KB)
- Français - FR (623 KB)
- Deutsch - DE (623 KB)
- Norwegian - NO (623 KB)
- Español - ES (623 KB)
- Swedish - SV (623 KB)
- Italian - IT (623 KB)
- Portuguese - PT (623 KB)
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Handling Instructions (2659 KB)
References
[1]. Dowlati A, et al. Combined inhibition of epidermal growth factor receptor and JAK/STAT pathways results in greater growth inhibition in vitro than single agent therapy. Mol Cancer Ther. 2004 Apr;3(4):459-63 [Content Brief]
[2]. Wang LH, et al. JAK3, STAT, and MAPK signaling pathways as novel molecular targets for the tyrphostin AG-490 regulation ofIL-2-mediated T cell response. J Immunol. 1999 Apr 1;162(7):3897-904. [Content Brief]
[3]. Davoodi-Semiromi A, et al. The tyrphostin agent AG490 prevents and reverses type 1 diabetes in NOD mice. PLoS One. 2012;7(5):e36079. [Content Brief]
[4]. Cheppudira BP, et al. Anti-hyperalgesic effects of AG490, a Janus kinase inhibitor, in a rat model of inflammatory pain. Biomed Rep. 2015 Sep;3(5):703-706. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.3979 mL | 16.9895 mL | 33.9789 mL | 84.9473 mL |
| 5 mM | 0.6796 mL | 3.3979 mL | 6.7958 mL | 16.9895 mL | |
| 10 mM | 0.3398 mL | 1.6989 mL | 3.3979 mL | 8.4947 mL | |
| 15 mM | 0.2265 mL | 1.1326 mL | 2.2653 mL | 5.6632 mL | |
| 20 mM | 0.1699 mL | 0.8495 mL | 1.6989 mL | 4.2474 mL | |
| 25 mM | 0.1359 mL | 0.6796 mL | 1.3592 mL | 3.3979 mL | |
| 30 mM | 0.1133 mL | 0.5663 mL | 1.1326 mL | 2.8316 mL | |
| 40 mM | 0.0849 mL | 0.4247 mL | 0.8495 mL | 2.1237 mL | |
| 50 mM | 0.0680 mL | 0.3398 mL | 0.6796 mL | 1.6989 mL | |
| 60 mM | 0.0566 mL | 0.2832 mL | 0.5663 mL | 1.4158 mL | |
| 80 mM | 0.0425 mL | 0.2124 mL | 0.4247 mL | 1.0618 mL | |
| 100 mM | 0.0340 mL | 0.1699 mL | 0.3398 mL | 0.8495 mL |
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