Referencia número: HY-N0751

Scutellarin

24 Cited Publications

Revisión del cliente

Based on 24 publication(s) in Google Scholar

Scutellarin, an active flavone isolated from Scutellaria baicalensis, can down-regulates the STAT3/Girdin/Akt signaling in HCC cells, and inhibits RANKL-mediated MAPK and NF-κB signaling pathway in osteoclasts. Scutellarin is active against HIV-1IIIB, HIV-1(74V) and HIV-1KM018 with EC₅₀s of 26 μM, 253 μM and 136 μM, respectively.

Para uso exclusivo en investigación. No vendemos a pacientes.

Pureza: 99.57%
No. CAS: 27740-01-8
Fòrmula: C₂₁H₁₈O₁₂
Peso molecular:462.36

Almacenamiento:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 1 year , -20°C, 6 months

Scutellarin Chemical Structure

No. CAS: 27740-01-8

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Actividad biológica
- Descripciòn
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (24)
Solvente y solubilidad
- In Vitro
- In Vivo
Protocolo
Pureza y Documentación
Referencias
Help & FAQs
Classifications
Bioz

Actividad biológica

Descripciòn

IC₅₀ & Target^[1]

STAT3

HIV-1

Ver todos los productos específicos de isoformas STAT :

Ver todos los productos específicos de isoformas Akt :

Ver todos los productos específicos de isoformas HIV :

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	>50 μM Compound: 1	Antiproliferative activity against human A549 cells after 72 hrs by MTT assay Antiproliferative activity against human A549 cells after 72 hrs by MTT assay	[PMID: 30243153]
Bel-7402	IC₅₀	>50 μM Compound: 1	Antiproliferative activity against human Bel7402 cells after 72 hrs by MTT assay Antiproliferative activity against human Bel7402 cells after 72 hrs by MTT assay	[PMID: 30243153]
CHO	IC₅₀	63.4 μM Compound: 3	Displacement of [3H]LSD from human 5HT7 receptor expressed in CHO cells Displacement of [3H]LSD from human 5HT7 receptor expressed in CHO cells	[PMID: 12713409]
HCT-116	IC₅₀	77 μM Compound: Scutellarin	Antiproliferative activity against human HCT116 cells after 72 hrs by MTT assay Antiproliferative activity against human HCT116 cells after 72 hrs by MTT assay	[PMID: 28458133]
HepG2	IC₅₀	56.09 μM Compound: Scutellarin	Antiproliferative activity against human HepG2 cells after 72 hrs by MTT assay Antiproliferative activity against human HepG2 cells after 72 hrs by MTT assay	[PMID: 28458133]
L02	IC₅₀	>100 μM Compound: Scutellarin	Antiproliferative activity against human LO2 cells after 72 hrs by MTT assay Antiproliferative activity against human LO2 cells after 72 hrs by MTT assay	[PMID: 28458133]
L02	IC₅₀	>50 μM Compound: 1	Cytotoxicity against human LO2 cells assessed as decrease in cell viability after 72 hrs by MTT assay Cytotoxicity against human LO2 cells assessed as decrease in cell viability after 72 hrs by MTT assay	[PMID: 30243153]
MCF7	IC₅₀	>100 μM Compound: Scutellarin	Antiproliferative activity against human MCF7 cells after 72 hrs by MTT assay Antiproliferative activity against human MCF7 cells after 72 hrs by MTT assay	[PMID: 28458133]
MCF7	IC₅₀	>50 μM Compound: 1	Antiproliferative activity against human MCF7 cells after 72 hrs by MTT assay Antiproliferative activity against human MCF7 cells after 72 hrs by MTT assay	[PMID: 30243153]
PC-3	IC₅₀	72.9 μM Compound: Scutellarin	Antiproliferative activity against human PC3 cells after 72 hrs by MTT assay Antiproliferative activity against human PC3 cells after 72 hrs by MTT assay	[PMID: 28458133]

In Vitro

Scutellarin treatment significantly reduces HepG2 cell viability in a dose-dependent manner, and inhibits migration and invasion of HCC cells in vitro. Scutellarin treatment significantly reduces STAT3 and Girders of actin filaments (Girdin) expression, STAT3 and Akt phosphorylation in HCC cells. Introduction of STAT3 overexpression restores the scutellarin-downregulated Girdin expression, Akt activation, migration and invasion of HCC cells. Furthermore, induction of Girdin overexpression completely abrogates the inhibition of scutellarin on the Akt phosphorylation, migration and invasion of HCC cells. Scutellarin can inhibit HCC cell metastasis in vivo, and migration and invasion in vitro by down-regulating the STAT3/Girdin/Akt signaling^[1]. Scutellarin selectively enhances Akt phosphorylation^[2]. Scutellarin is a putative therapeutic agent as it has been found to not only suppress microglial activation thus ameliorating neuroinflammation, but also enhance astrocytic reaction. Acutellarin amplifies the astrocytic reaction by upregulating the expression of neurotrophic factors among others thus indicating its neuroprotective role. Remarkably, the effects of scutellarin on reactive astrocytes are mediated by activated microglia supporting a functional "cross-talk" between the two glial types^[3]. Scutellarin can suppress RANKL-mediated osteoclastogenesis, the function of osteoclast bone resorption, and the expression levels of osteoclast-specific genes (tartrate-resistant acid phosphatase (TRAP), cathepsin K, c-Fos, NFATc1). Further investigation indicates that Scutellarin can inhibit RANKL-mediated MAPK and NF-κB signaling pathway, including JNK1/2, p38, ERK1/2, and IκBα phosphorylation^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Scutellarin (50 mg/kg/day) significantly mitigates the lung and intrahepatic metastasis of HCC tumors in vivo. The numbers of the lung and intrahepatic metastatic tumors in the scutellarin-treated group are significantly less than that in the controls^[1]. The rats treated with Scutellarin display a significant alleviation in neurobehavioral deficits compared to the SAH group. Scutellarin enhanced eNOS expression compared with SAH rats^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

No. CAS 27740-01-8
Appearance Solid
Peso molecular 462.36
Fòrmula C₂₁H₁₈O₁₂
Color Light yellow to green yellow
SMILES

O=C(C=C(C1=CC=C(O)C=C1)OC2=CC(O[C@@H]([C@@H]([C@@H](O)[C@@H]3O)O)O[C@@H]3C(O)=O)=C4O)C2=C4O
Structure Classification
- Flavonoids
- Flavones
- Phenols
- Polyphenols
Initial Source
- Plants
- Labiatae
Envío

Room temperature in continental US; may vary elsewhere.

Almacenamiento

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	1 year
	-20°C	6 months

Publications (24)

All Cell Proliferation/Viability AssayELISAWBHistological Imaging/Staining

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Journal Impact Factor

Pharmacol Res

Scutellarin inhibits pyroptosis via selective autophagy degradation of p30/GSDMD and suppression of ASC oligomerization. [Abstract]

2025 Jan 15:107605. PMID: 39824372

Scutellarin purchased from MedChemExpress. Usage Cited in: Pharmacol Res. 2025 Jan 15:107605. [Abstract]

THP-1 and HEK-293T were treated with Scutellarin (SCU) at gradient concentration (50 μM, 100 μM and 200 μM) for 24 h, cell viability was detected by CCK-8 assay.

Scutellarin purchased from MedChemExpress. Usage Cited in: Pharmacol Res. 2025 Jan 15:107605. [Abstract]

THP-1 cells were primed with 500 ng/mL LPS for 4 h, followed by a 1-hour treatment with varying concentrations of Scutellarin (SCU, 50 μM and 100 μM) and a subsequent 30-minute 10 μM NIG stimulation to activate the NLRP3 inflammasome. The supernatants of THP-1 cells were collected for IL-18 detection using an ELISA kit.

Scutellarin purchased from MedChemExpress. Usage Cited in: Pharmacol Res. 2025 Jan 15:107605. [Abstract]

FLAG-NLRP3, FLAG-ASC, HA-caspase-1, and FLAG-IL-1β were co-transfected into HEK-293T cells. Five hours later, the culture medium was replaced, and 100 μM Scutellarin (SCU) was added. After 24 hours, the cells underwent DSS cross-linking, and protein samples were collected to detect inflammasome components via Western blotting.

Scutellarin purchased from MedChemExpress. Usage Cited in: Pharmacol Res. 2025 Jan 15:107605. [Abstract]

Spleens were used to prepare pathological sections and perform HE staining. These sections revealed that Scutellarin (SCU, 10 mg/kg; i.p.) inhibited LPS-induced inflammatory cell infiltration.
Food Chem

Effects of sun drying combined with baking processes on the flavor quality of Chongqing Tuocha raw tea. [Abstract]

2025 Dec 30:497:146992. PMID: 41285060
Food Chem

Flavonoid-mediated metabolic underpinning quality variation in red bud-sport pear mutants. [Abstract]

2025 May 31:489:144992. PMID: 40466530
Cell Death Dis

Scutellarin ameliorates pulmonary fibrosis through inhibiting NF-κB/NLRP3-mediated epithelial-mesenchymal transition and inflammation. [Abstract]

2020 Nov 13;11(11):978. PMID: 33188176
Phytomedicine

Fangchinoline suppresses nasopharyngeal carcinoma progression by inhibiting SQLE to regulate the PI3K/AKT pathway dysregulation. [Abstract]

2025 May:140:156484. PMID: 40090046
J Transl Med

IFN-γ-mediated inhibition of JAK/STAT signaling via nano-scutellarin treatment is an efficient strategy for ameliorating liver fibrosis. [Abstract]

2025 Feb 17;23(1):195. PMID: 39962553
Phytother Res

Scutellarin Attenuates Lipopolysaccharide-Induced Acute Lung Injury in Mice by Inhibiting M1 Macrophage Polarization via the GBP2/JAK2/STAT3 Signaling Pathway. [Abstract]

2025 Sep 18. PMID: 40968089
Phytother Res

Scutellarin ameliorates hepatic lipid accumulation by enhancing autophagy and suppressing IRE1α/XBP1 pathway. [Abstract]

2022 Jan;36(1):433-447. PMID: 34859513
Ind Crops Prod

2025 Dec 11;239:122458.
Inflammation

Scutellarin Alleviates Ovalbumin-Induced Airway Remodeling in Mice and TGF-β-Induced Pro-fibrotic Phenotype in Human Bronchial Epithelial Cells via MAPK and Smad2/3 Signaling Pathways. [Abstract]

2024 Jun;47(3):853-873. PMID: 38168709
Bioengineered

Preparation and characterization of scutellarin loaded on ultradeformable nano-liposomes scutellarin EDTMP (S-UNL-E) and in vitro study of its osteogenesis. [Abstract]

2022 Jan;13(1):1013-1024. PMID: 34974800
J Mater Sci

2026 Apr 27;61(22):15999-16015.
Med Oncol

Scutellarin triggers ferroptosis in ovarian cancer cells via inhibiting AKT/mTOR and JAK2/STAT3 pathways. [Abstract]

2025 Nov 25;43(1):24. PMID: 41288840
Toxicol Appl Pharmacol

Scutellarin attenuates keloid fibroblast progression by targeting EGFR/PI3K/AKT signaling: An integrated network pharmacology and in vitro experimental study. [Abstract]

2025 Oct 9:505:117564. PMID: 41067505
Front Biosci (Landmark Ed)

Scutellarin Attenuates Pro-Inflammatory Foam Cell Formation and Facilitates M2 Polarization in Microglia during Copper Homeostasis Imbalance via the MAPK Signaling Pathway. [Abstract]

2025 Mar 6;30(3):36255. PMID: 40152387
Cell Biol Int

Scutellarin-mediated autophagy activates exosome release of rat nucleus pulposus cells by positively regulating Rab8a via the PI3K/PTEN/Akt pathway. [Abstract]

2022 Oct;46(10):1588-1603. PMID: 35762224
Vet Microbiol

The Chinese medicine monomer Schisandrin C inhibits PRRSV infection by regulating the OGT-PI3K/AKT/mTOR signaling pathway. [Abstract]

2026 May:316:110992. PMID: 41865607
PLoS One

Scutellarin combined with lidocaine exerts antineoplastic effect in human glioma associated with repression of epidermal growth factor receptor signaling. [Abstract]

2025 Jan 31;20(1):e0318031. PMID: 39888904
Tissue Cell

Scutellarin reduces apoptosis in R28 cells induced by continuous hydrostatic pressure combined with oxygen-glucose deprivation through the Keap1/Nrf2/NF-κB pathway. [Abstract]

2025 Nov 17:99:103234. PMID: 41252784
J Nat Med

Analysis of compound-compound interactions between berberine and baicalin derivatives. [Abstract]

2025 Nov 23. PMID: 41276774
Tissue Cell

Scutellarin alleviates tensile stress-induced proliferation and migration of venous smooth muscle cells via mediating the p38 MAPK pathway. [Abstract]

2024 Apr:87:102300. PMID: 38211409
Exp Ther Med

Scutellarin ameliorates ischemia/reperfusion injury‑induced cardiomyocyte apoptosis and cardiac dysfunction via inhibition of the cGAS‑STING pathway. [Abstract]

2023 Feb 17;25(4):155. PMID: 36911381
Thorac Cancer

Scutellarin suppresses proliferation and promotes apoptosis in A549 lung adenocarcinoma cells via AKT/mTOR/4EBP1 and STAT3 pathways. [Abstract]

2019 Mar;10(3):492-500. PMID: 30666790

Scutellarin purchased from MedChemExpress. Usage Cited in: Thorac Cancer. 2019 Mar;10(3):492-500. [Abstract]

A549 cells are treated with Scutellarin (SCU) (0, 200, 400, 600 μM) for 24 hours. Western blotting analyses are used to detect the expression of proteins in the STAT3 pathways, cyclin D1, and cyclin E.
Nephrology (Carlton)

Scutellarin protects the kidney from ischemia/reperfusion injury by targeting Nrf2. [Abstract]

2022 Aug;27(8):690-700. PMID: 35638402

Solvente y solubilidad

In Vitro:

DMSO : 100 mg/mL (216.28 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.1628 mL

10.8141 mL

21.6282 mL

5 mM

0.4326 mL

2.1628 mL

4.3256 mL

10 mM

0.2163 mL

1.0814 mL

2.1628 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Calculadora de molaridad

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculadora de dilución

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 0.5% CMC-Na/saline water

Solubility: 10 mg/mL (21.63 mM); Suspended solution; Need ultrasonic

Protocol 2

Add each solvent one by one: 15% Cremophor EL 85% Saline

Solubility: 5 mg/mL (10.81 mM); Suspended solution; Need ultrasonic

Protocolo

Cell Assay^[1]

HepG2 cells (1×10⁵/well) are cultured in 96-well plates and treated in triplicate with scutellarin at concentrations of 5, 10, 20, 30, and 100 μM or vehicle alone for 24 h. The cellular viability is tested by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and is expressed as a percentage of proliferation versus controls.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration^[1]

To establish an orthotopic liver xenograft model, individual mice are anesthetized with isoflurane and a small incision is made in their abdomen. Individual mice are injected with 2×10⁶ SK-Hep1 cells in 30 μL Matrigel into their left lobe of the liver. Twenty-four hours after orthotopic liver implantation, the mice are randomized and injected intraperitoneally with scutellarin (50 mg/kg/day) or vehicle (0.9% NaCl, normal saline) daily for 35 consecutive days (n=10 per group). Subsequently, the mice are sacrificed, and their lungs and livers are excised, fixed in 10% buffered formalin and paraffin-embedded for hematoxylin and eosin staining.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Pureza y Documentación

Select Batch:

Pureza: 99.98%

Assay: 99.98%

ee.: 99.98%

Ficha de datos (282 KB)
SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instrucciones de manejo (2659 KB)

Referencias

[1]. Ke Y, et al. Scutellarin suppresses migration and invasion of human hepatocellular carcinoma by inhibiting the STAT3/Girdin/Akt activity. Biochem Biophys Res Commun. 2016 Dec 18. pii: S0006-291X(16)32174-X [Content Brief]

[2]. Yang LL, et al. Differential regulation of baicalin and scutellarin on AMPK and Akt in promoting adipose cell glucose disposal. Biochim Biophys Acta. 2016 Nov 27;1863(2):598-606. [Content Brief]

[3]. Wu CY, et al. Scutellarin attenuates microglia-mediated neuroinflammation and promotes astrogliosis in cerebral ischemia - a therapeutic consideration. Curr Med Chem. 2016 Nov 18. [Epub ahead of print] [Content Brief]

[4]. Li Q, et al. Scutellarin attenuates vasospasm through the Erk5-KLF2-eNOS pathway after subarachnoid hemorrhage in rats. J Clin Neurosci. 2016 Dec;34:264-270 [Content Brief]

[5]. Zhao S, et al. Scutellarin inhibits RANKL-mediated osteoclastogenesis and titanium particle-induced osteolysis via suppression of NF-κB and MAPK signaling pathway. Int Immunopharmacol. 2016 Nov;40:458-465 [Content Brief]

[6]. Zhang GH, et al. The anti-HIV-1 effect of scutellarin. Biochem Biophys Res Commun. 2005 Sep 2;334(3):812-6. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.1628 mL	10.8141 mL	21.6282 mL	54.0704 mL
	5 mM	0.4326 mL	2.1628 mL	4.3256 mL	10.8141 mL
	10 mM	0.2163 mL	1.0814 mL	2.1628 mL	5.4070 mL
	15 mM	0.1442 mL	0.7209 mL	1.4419 mL	3.6047 mL
	20 mM	0.1081 mL	0.5407 mL	1.0814 mL	2.7035 mL
	25 mM	0.0865 mL	0.4326 mL	0.8651 mL	2.1628 mL
	30 mM	0.0721 mL	0.3605 mL	0.7209 mL	1.8023 mL
	40 mM	0.0541 mL	0.2704 mL	0.5407 mL	1.3518 mL
	50 mM	0.0433 mL	0.2163 mL	0.4326 mL	1.0814 mL
	60 mM	0.0360 mL	0.1802 mL	0.3605 mL	0.9012 mL
	80 mM	0.0270 mL	0.1352 mL	0.2704 mL	0.6759 mL
	100 mM	0.0216 mL	0.1081 mL	0.2163 mL	0.5407 mL

Keywords

Scutellarin

24 Cited Publications

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Scutellarin

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Publications Citing Use of MedChemExpress (MCE) Scutellarin

Ver todos los productos específicos de isoformas STAT

Ver todos los productos específicos de isoformas Akt

Ver todos los productos específicos de isoformas HIV

Actividad biológica

Chemical Information

Publications (24)

Solvente y solubilidad

Protocolo