TAT-EE3
Based on 1 Customer Validation
TAT-EE3 is a neuroprotective peptide which can uncouple TRPM2-NMDARs interaction. TAT-EE3 inhibits TRPM2-induced enhancement of NMDAR surface expression and current amplitude.TAT-EE3 protects neurons against ischemic injury in vitro and in vivo. TAT-EE3can be used for the study of ischemic stroke.
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- Pureza: 99.89%
- No. CAS: 2899218-49-4
- Fòrmula: C135H228N48O49S
- Peso molecular:3339.61
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Almacenamiento:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
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Actividad biológica
TAT-EE3 (10 μM, overnight) disrupts the interaction between TRPM2 and GluN2a/GluN2b in HEK293T cells and neurons, inhibits TRPM2-induced enhancement of NMDAR surface expression and current amplitude[1].
TAT-EE3 (10 μM, overnight) reduces OGD-induced intracellular Ca2+ overload, neuronal death, and mitochondrial depolarization in wild-type neurons, but has no effect on gM2KO neurons[1].
TAT-EE3 (10 μM, 30-90 min) eliminates PMA-induced NMDAR current potentiation in wild-type neurons, without additional inhibition in gM2KO neurons[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Focal cerebral ischemia models were constructed by performing right middle cerebral artery occlusion (MCAO) on 8-9 week-old male mice, with 120 minutes of occlusion followed by 24 hours of reperfusion, or 60 minutes of occlusion followed by 7 days of reperfusion[1]
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Dosage:0.33 mg/kg (100 nmol/kg)
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Administration:i.p. repeated every 12 h after MCAO for 7 days
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Result:Reduced infarct volume.
Inhibited MCAO-induced increase in NMDAR surface expression in wild-type mice, but did not exert further Protective effects in neuron-specific TRPM2 knockout (nM2KO) mice.
Improved long-term neurological deficit scores and rotarod test performance in mice.
Maintained the phosphorylation levels of ERK1/2 and CREB in the brain.
Chemical Information
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No. CAS 2899218-49-4
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Appearance Solid
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Peso molecular 3339.61
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Fòrmula C135H228N48O49S
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Color White to off-white
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Sequence
Tyr-Gly-Arg-Lys-Lys-Arg-Arg-Gln-Arg-Arg-Arg-Glu-Glu-Asp-Thr-Asp-Ser-Ser-Glu-Glu-Met-Leu-Ala-Leu-Ala-Glu-Glu
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Sequence Shortening
YGRKKRRQRRREEDTDSSEEMLALAEE
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvente y solubilidad
H2O : 5.88 mg/mL (1.76 mM; ultrasonic and adjust pH to 9 with NH3·H2O)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Pureza y Documentación
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Ficha de datos (280 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 0.2994 mL | 1.4972 mL | 2.9944 mL | 7.4859 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
TAT-EE3 Related Classifications
HY-P11117 Related Classifications
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