HDAC6 degrader-1
Based on 1 Customer Validation
HDAC6 degrader-1 is the PROTAC degrader for HDAC6 with a DC50 of 3.8 nM in cell MM.1S.
(Pink: HDAC6 ligand (HY-16699); Blue: Cereblon ligand (HY-10984); Black: linker (HY-140213)).
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- Pureté : 99.58%
- CAS No.: 2439058-23-6
- Formule: C40H45N9O9
- Masse moléculaire:795.84
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Stockage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
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Activité biologique
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| MM1.S | GI50 |
1.21 μM
Compound: 10c; NP8
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Growth inhibition of human MM1.S cells
Growth inhibition of human MM1.S cells
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[PMID: 32871345] |
In Vitro
HDAC6 degrader-1 (Compound NP8) (0-10 μM, 72 h) inhibits the cell viability of MM.1S with GI50 of 1.21 μM[1].
HDAC6 degrader-1 is the PROTAC degrader for HDAC6 with a DC50 of 3.8 nM in cell MM.1S[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:MM.1S
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Concentration:0-10 μM
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Incubation Time:72 h
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Result:Inhibited the cell viability.
Chemical Information
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CAS No. 2439058-23-6
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Appearance Solid
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Masse moléculaire 795.84
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Formule C40H45N9O9
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Color Light yellow to yellow
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SMILES
O=C(C1=CC=C(CN(CCCCC2=CN(CCOCCOCCNC3=CC=CC(C(N4C5C(NC(CC5)=O)=O)=O)=C3C4=O)N=N2)C(NC6=CC=CC=C6)=O)C=C1)NO
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvant et solubilité
In Vitro:
DMSO : 100 mg/mL (125.65 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (3.14 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureté et documentation
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Fiche technique (270 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instruction de manipulation (2659 KB)
Références
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.2565 mL | 6.2827 mL | 12.5653 mL | 31.4133 mL |
| 5 mM | 0.2513 mL | 1.2565 mL | 2.5131 mL | 6.2827 mL | |
| 10 mM | 0.1257 mL | 0.6283 mL | 1.2565 mL | 3.1413 mL | |
| 15 mM | 0.0838 mL | 0.4188 mL | 0.8377 mL | 2.0942 mL | |
| 20 mM | 0.0628 mL | 0.3141 mL | 0.6283 mL | 1.5707 mL | |
| 25 mM | 0.0503 mL | 0.2513 mL | 0.5026 mL | 1.2565 mL | |
| 30 mM | 0.0419 mL | 0.2094 mL | 0.4188 mL | 1.0471 mL | |
| 40 mM | 0.0314 mL | 0.1571 mL | 0.3141 mL | 0.7853 mL | |
| 50 mM | 0.0251 mL | 0.1257 mL | 0.2513 mL | 0.6283 mL | |
| 60 mM | 0.0209 mL | 0.1047 mL | 0.2094 mL | 0.5236 mL | |
| 80 mM | 0.0157 mL | 0.0785 mL | 0.1571 mL | 0.3927 mL | |
| 100 mM | 0.0126 mL | 0.0628 mL | 0.1257 mL | 0.3141 mL |
HDAC6 degrader-1 Related Classifications
HY-132998 Related Classifications
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