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Methotrexate hydrate

(Synonyms: Amethopterin hydrate; CL14377 hydrate; WR19039 hydrate)
77 Cited Publications
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Based on 77 publication(s) in Google Scholar

Methotrexate hydrate is a substance that affects cell growth and division. It is commonly used to fight certain diseases, especially cancer and autoimmune diseases. It works by inhibiting the process by which cells synthesize DNA.

For research use only. We do not sell to patients.

  • Purity: 99.57%
  • CAS No.: 133073-73-1
  • Formula: C20H22N8O5.xH2O
  • Storage:

    4°C, sealed storage, away from moisture and light

    * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

  • Methotrexate hydrate Chemical Structure
    Methotrexate hydrate Chemical Structure
  • Experimental Validation Results for Methotrexate hydrate
  • Experimental Validation Results for Methotrexate hydrate
  • Experimental Validation Results for Methotrexate hydrate
  • Experimental Validation Results for Methotrexate hydrate
  • Experimental Validation Results for Methotrexate hydrate
CAS No.: 133073-73-1
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Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
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Publications Citing Use of MedChemExpress (MCE) Methotrexate hydrate

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Cell Imaging/StainingCell Proliferation/Viability AssayRT-PCRCell Migration/Invasion Assay
  • Cell Imaging/Staining
  • RT-PCR
  • Cell Proliferation/Viability Assay
  • Cell Migration/Invasion Assay
  • Cell Proliferation/Viability Assay

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100 mg

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  • Methotrexate hydrate Chemical Structure
    Methotrexate hydrate Chemical Structure
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    Methotrexate hydrate purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Feb 28;16(1):2071. [Abstract]

    Customer Validation
     OIS cells were infected with shCtrl orshACSS2 followed by selection and treated with or without MTX (40 μM) for 2 days. Cells were stained with γH2AX.

  • 1/16

    Methotrexate hydrate purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Feb 28;16(1):2071. [Abstract]

    Customer Validation
     OIS cells were infected with shCtrl orshACSS2 followed by selection and treated with or without MTX (40 μM) for 2 days. The expression of SASP genes was analysed by qRT–PCR.

  • 1/16

    Methotrexate hydrate purchased from MedChemExpress. Usage Cited in: J Adv Res. 2025 Aug:74:609-620. [Abstract]

    Customer Validation
     The cell viability of MH7A cells treated with methotrexate (1 μM) for 24 h was determined by the CCK-8 method.

  • 1/16

    Methotrexate hydrate purchased from MedChemExpress. Usage Cited in: J Adv Res. 2025 Aug:74:609-620. [Abstract]

    Customer Validation
     The cell migration of MH7A cells treated with methotrexate (1 μM) for 24 h.

  • 1/16

    Methotrexate hydrate purchased from MedChemExpress. Usage Cited in: J Clin Invest. 2023 Jul 3;133(13):e169993. [Abstract]

    Customer Validation
     A CellTiter-Glo® luminescent cell viability assay was performed on CAL51 WT and MLL4 KO cells, which were treated with 0, 0.04, or 0.4 μM Methotrexate (MTX) in the presence of H2O, 50 μM thymidine, or 50 μM inosine.

  • Methotrexate hydrate