製品番号: HY-N2368

Arecaidine

1 Cited Publications

カスタマーレビュー

Based on 1 publication(s) in Google Scholar

Arecaidine is a GABA transport system inhibitor. Arecaidine inhibits the proliferation of oral mucosal fibroblasts, increases the secretion of IL-6, TGF-β and TNF-α in cells, downregulates the expression of PPAR-γ and PCK1 in cells, and upregulates the expression of TGF-β1. Arecaidine inhibits the uptake of γ-aminobutyric acid and β-alanine by the central nervous system of cats. Arecaidine inhibits hPAT1-mediated L-[³H]proline uptake in cells. Arecaidine can be used in research related to neurological diseases.

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

純度: 99.63%
CAS 番号: 499-04-7
分子式: C₇H₁₁NO₂
分子量:141.17

保管条件:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Arecaidine 構造式

CAS 番号: 499-04-7

容量

価格（税別）

在庫状況

数量

Amount: USD 0.00

* アイテムを追加する前、数量をご選択ください

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

生物活性

製品説明

Interleukin Related アイソフォーム固有の製品をすべて表示 :

TGF-β Receptor アイソフォーム固有の製品をすべて表示 :

TNF Receptor アイソフォーム固有の製品をすべて表示 :

PPAR アイソフォーム固有の製品をすべて表示 :

体外実験

Arecaidine exhibits strong binding affinity for PPAR-γ, PCK1 and TGF-β1 proteins, with binding energies of -5.1 kJ/mol, -5.6 kJ/mol and -5.6 kJ/mol^[1].
Arecaidine (0-120 μg/mL; 24-48 h) inhibits the proliferation of human oral mucosal fibroblasts (hOMF) and increases the secretion of IL-6, TGF-β and TNF-α in cells^[1].
Arecaidine (100-120 μg/mL; 48 h) downregulates the mRNA and protein expression of PPAR-γ and PCK1, while upregulates the mRNA and protein expression of TGF-β1, in human oral mucosal fibroblasts (hOMF)^[1].
Arecaidine (10 min) inhibits the uptake of GABA and β-alanine, but not that of glycine, in cat spinal cord slices, with an IC₅₀ of 89 μM for GABA and 193 μM for β-alanine^[2].
Arecaidine (10 min) inhibits the uptake of GABA and β-alanine in cat cerebellar slices, with an IC₅₀ value of 76 μM for GABA and 354 μM for β-alanine^[2].
Arecaidine (10 min) inhibits the uptake of GABA and β-alanine in rat cerebral cortex slices, with an IC₅₀ value of 122 μM for GABA and 208 μM for β-alanine^[2].
Arecaidine (0.1-100 mM; 10 min) competitively inhibits hPAT1-mediated uptake of L-[³H]proline in Caco-2 cells, with a K_i value of 7.3 mM^[3].
Arecaidine (0.1-100 mM; 10 min) inhibits hPAT1-mediated L-[³H]proline uptake in transiently transfected HeLa cells, with a K_i value of 3.8 mM^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

human oral mucosal fibroblasts (hOMF)
Concentration:

0, 40, 60, 80, 100, 120 μg/mL
Incubation Time:

24 h; 48 h
Result:

Did not significantly alter hOMF cell viability after 24 h of treatment across all tested concentrations.
Significantly inhibited hOMF cell proliferation at 100 μg/mL and 120 μg/mL after 48 h of treatment.

ELISA Assay^[1]

Cell Line:

human oral mucosal fibroblasts (hOMF)
Concentration:

100, 120 μg/mL
Incubation Time:

48 h
Result:

Significantly elevated IL-6, TGF-β, TNF-α levels.

Real Time qPCR^[1]

Cell Line:

human oral mucosal fibroblasts (hOMF)
Concentration:

100, 120 μg/mL
Incubation Time:

48 h
Result:

Significantly downregulated PPAR-γ, PCK1, TGF-β1 mRNA expression.

Western Blot Analysis^[1]

Cell Line:

human oral mucosal fibroblasts (hOMF)
Concentration:

100 μg/mL
Incubation Time:

48 h
Result:

Significantly reduced PPAR-γ, PCK1, TGF-β1 protein expression.

体内実験

Topical administration of arecaidine (1 mg/mL; once daily for 16 weeks) to the buccal mucosa induces oral submucous fibrosis in rats, which is characterized by reduced mouth opening, increased collagen deposition, elevated TGF-β expression, and dysregulated PPAR-γ signaling pathway^[1].
Arecaidine (0.2 M solution; electrophoretic) selectively potentiates the inhibitory effects of GABA and β-alanine on cat spinal cord neurons, and enhances the effect of GABA on cat cerebellar Purkinje cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Sprague-Dawley (SD) (male, 6-8 weeks old, 200 g)^[1]
Dosage:

1 mg/mL
Administration:

topical application to buccal mucosa; once daily; 16 weeks
Result:

Reduced passive mouth opening to 23.97 mm, a statistically significant reduction compared to the control group.
Increased collagen volume fraction significantly higher than the control group.
Increased TGF-β protein expression statistically significantly compared to the control group.
Identified 100 significantly differentially expressed genes, including downregulation of PPAR-γ, PCK1, pdk4, plin5, Hmgcs2, UCP3, and Angptl4, and upregulation of TGF-β1 and FOS.
Demonstrated binding energies < -5.0 kJ/mol with PPAR-γ, PCK1, and TGF-β1, indicating strong binding affinity.

Animal Model:

unspecified strain (anaesthetised with pentobarbitone sodium)^[2]
Dosage:

0.2M solution
Administration:

electrophoretic; variable duration
Result:

Selectively potentiated the inhibitory effects of GABA and β-alanine on cat spinal cord neurons, and enhanced the effect of GABA on cat cerebellar Purkinje cells.

化学情報

CAS 番号 499-04-7
性状 Solid
分子量 141.17
分子式 C₇H₁₁NO₂
Color White to off-white
SMILES

O=C(C1=CCCN(C)C1)O
Structure Classification
- Alkaloids
- Pyridine Alkaloids
Initial Source
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (1)

All

Sort by:

Journal Impact Factor

Int J Mol Sci

Oxidative Stress and Endoplasmic Reticulum Stress Contributes to Arecoline and Its Secondary Metabolites-Induced Dyskinesia in Zebrafish Embryos. [Abstract]

2023 Mar 28;24(7):6327. PMID: 37047326

溶剤 & 溶解度

体外:

H₂O : 250 mg/mL (1770.91 mM; Need ultrasonic)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

7.0837 mL

35.4183 mL

70.8366 mL

5 mM

1.4167 mL

7.0837 mL

14.1673 mL

10 mM

0.7084 mL

3.5418 mL

7.0837 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

モル濃度計算機

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

濃度濃度

体積体積

分子量分子量

希釈計算機

濃度 _(開始) × 体積 _(開始) = 濃度 _(終了) × 体積 _(終了)

濃度 _(開始) 濃度 _(開始)

体積 _(開始) 体積 _(開始)

濃度 _(終了) 濃度 _(終了)

体積 _(終了) 体積 _(終了)

体内:

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS

Solubility: 100 mg/mL (708.37 mM); Clear solution; Need ultrasonic

純度とドキュメンテーション

バッチを選択:

純度: 99.98%

Assay: 99.98%

ee.: 99.98%

データシート (279 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
元素分析報告書 (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
取扱説明書 (2659 KB)

参考文献

[1]. Zeng F, et al. Inhibitory role of arecaidine on PPARγ signaling in oral mucosa: Mechanistic insights from transcriptome and experimental analysis. Toxicon. 2025;262:108403. [Content Brief]

[2]. Lodge D, et al. Effects of the Areca nut constituents arecaidine and guvacine on the action of GABA in the cat central nervous system. Brain Res. 1977;136(3):513-522. [Content Brief]

[3]. Voigt V, et al. Transport of the areca nut alkaloid arecaidine by the human proton-coupled amino acid transporter 1 (hPAT1). J Pharm Pharmacol. 2013;65(4):582-590. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O	1 mM	7.0837 mL	35.4183 mL	70.8366 mL	177.0915 mL
	5 mM	1.4167 mL	7.0837 mL	14.1673 mL	35.4183 mL
	10 mM	0.7084 mL	3.5418 mL	7.0837 mL	17.7091 mL
	15 mM	0.4722 mL	2.3612 mL	4.7224 mL	11.8061 mL
	20 mM	0.3542 mL	1.7709 mL	3.5418 mL	8.8546 mL
	25 mM	0.2833 mL	1.4167 mL	2.8335 mL	7.0837 mL
	30 mM	0.2361 mL	1.1806 mL	2.3612 mL	5.9030 mL
	40 mM	0.1771 mL	0.8855 mL	1.7709 mL	4.4273 mL
	50 mM	0.1417 mL	0.7084 mL	1.4167 mL	3.5418 mL
	60 mM	0.1181 mL	0.5903 mL	1.1806 mL	2.9515 mL
	80 mM	0.0885 mL	0.4427 mL	0.8855 mL	2.2136 mL
	100 mM	0.0708 mL	0.3542 mL	0.7084 mL	1.7709 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Keywords

Arecaidine

1 Cited Publications

100 mg お問い合わせ

Subscribe to the

MedChemExpress

Newsletter

製品名:
製品番号:
数量:
日本の代理先をお選びください:

製品名:
CAS 番号:
生物種:
純度:
数量:

Arecaidine

Apoptosis Compound Library

Cell Cycle/DNA Damage Compound Library

Immunology/Inflammation Compound Library

Membrane Transporter/Ion Channel Compound Library

Metabolism/Protease Compound Library

Neuronal Signaling Compound Library

TGF-beta/Smad Compound Library

Natural Product Library

Anti-Aging Compound Library

Differentiation Inducing Compound Library

Medicine Food Homology Compound Library

Orally Active Compound Library

Neurotransmitter Receptor Compound Library

Traditional Chinese Medicine Active Compound Library

Anti-Alzheimer's Disease Compound Library

Alkaloids Library

Anti-Pancreatic Cancer Compound Library

Anti-Parkinson's Disease Compound Library

Neurodegenerative Disease-related Compound Library

Anti-Obesity Compound Library

Angiogenesis-Related Compound Library

Transcription Factor-Targeted Library

Anti-Liver Cancer Compound Library

Antidepressant Compound Library

Anti-inflammatory Traditional Chinese Medicine Active Compound Library

Plant-Sourced Natural Product Library

GABA Receptor Compound Library

Human Metabolite Library

Anti-Prostate Cancer Compound Library

Nuclear Receptor Compound Library

Osteogenesis Compound Library

Cancer Stem Cells Compound Library

Pain-Related Compound Library

Metabolic Enzyme Compound Library

Membrane Protein-targeted Compound Library

Membrane Receptor-targeted Compound Library

Autoimmune Disease Compound Library

Ion Channel Compound Library

Multi-Target Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Natural Product Library Plus

Natural Product and Natural Product-Like Compound Library

Bioactive Compound Library

Miao Ethnicity Medicine Compound Library

Tibetan Medicine Compound Library

Heat-Clearing and Detoxification Traditional Chinese Medicine Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

RO5 Drug-like Natural Product Library

High-Throughput Bioactive Compound Library

High-Throughput Natural Product Library

Ancient Chinese Classical Formulas Traditional Chinese Medicine Active Compound Library

Mass Spectrometry Human Metabolite Library

Diarrhea-Related Traditional Chinese Medicine Active Compound Library

Mongolian Medicine Compound Library

Mass Spectrometry Natural Product Library

3D Diverse Fragment Library

MedChemExpress（MCE）の使用を引用している文献 Arecaidine

Interleukin Related アイソフォーム固有の製品をすべて表示

TGF-β Receptor アイソフォーム固有の製品をすべて表示

TNF Receptor アイソフォーム固有の製品をすべて表示

PPAR アイソフォーム固有の製品をすべて表示

生物活性

化学情報

Publications (1)

溶剤 & 溶解度

純度とドキュメンテーション

参考文献

Help & FAQs

Complete Stock Solution Preparation Table

Arecaidine 関連分類

HY-N2368 関連分類