HBP-10
HBP-10 is an anticancer agent containing a Michael acceptor. HBP-10 induces intracellular ROS accumulation, triggering IRE1α-JNK/CHOP-mediated ER stress, γ-H2AX-positive DNA damage, and caspase-dependent apoptosis (apoptosis). HBP-10's above effects are attenuated by NAC (HY-B0215). HBP-10 exerts antiproliferative and cytotoxic effects on NSCLC cells and shows certain selectivity for NSCLC cells. HBP-10 can be used for research on EGFR-mutant non-small cell lung cancer.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 分子式: C14H7ClN4O2
- 分子量:298.68
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保管条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
製品説明
Cellular Effect
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Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| H1975 | IC50 |
45.15 μM
|
Inhibition of viability in human H1975 cells by CCK-8 assay after incubation with HBP-10 for 24 hrs.
Inhibition of viability in human H1975 cells by CCK-8 assay after incubation with HBP-10 for 24 hrs.
|
42697283 |
| HCC827 | IC50 |
69.54 μM
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Inhibition of viability in human HCC827 cells by CCK-8 assay after incubation with HBP-10 for 24 hrs.
Inhibition of viability in human HCC827 cells by CCK-8 assay after incubation with HBP-10 for 24 hrs.
|
42697283 |
| A549 | IC50 |
58.76 μM
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Inhibition of viability in human A549 cells by CCK-8 assay after incubation with HBP-10 for 24 hrs.
Inhibition of viability in human A549 cells by CCK-8 assay after incubation with HBP-10 for 24 hrs.
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42697283 |
| HEK293 | IC50 |
>200 μM
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Inhibition of viability in human HEK293 cells by CCK-8 assay after incubation with HBP-10 for 24 hrs with IC50 >200 μM.
Inhibition of viability in human HEK293 cells by CCK-8 assay after incubation with HBP-10 for 24 hrs with IC50 >200 μM.
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42697283 |
体外実験
HBP-10 (24 h) inhibits cell viability, with an IC50 of 45.15 μM in H1975 cells, 69.54 μM in HCC827 cells, 58.76 μM in A549 cells, and >200 μM in HEK293 cells[1].
HBP-10 (50 μM) induces DNA damage signaling in H1975 cells, as evidenced by increased γ-H2AX[1].
HBP-10 (50 μM) reduces viable H1975 cells and increases PI-positive and calcein-negative damaged cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:H1975 NSCLC cells
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Concentration:0, 10, 20 and 40 μM
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Incubation Time:7 days
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Result:Inhibited long-term colony-forming survival of H1975 NSCLC cells in a dose-dependent manner.
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Cell Line:H1975 cells
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Concentration:50 μM
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Incubation Time:0, 0.5, 1, 3, 6, 12 h
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Result:Exposure to this compound increased BiP/GRP78 expression, enhanced IRE1α phosphorylation and accumulation, increased JNK phosphorylation, and elevated CHOP expression after 6 h.
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Cell Line:H1975 cells
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Concentration:50 μM
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Incubation Time:24 h
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Result:Induced extensive chromatin condensation and nuclear fragmentation.
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Cell Line:H1975 cells
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Concentration:0, 10, 50, 100 μM
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Incubation Time:0, 6, 12, 24 h
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Result:Exposure to this compound induced cleavage of pro-caspase-2 and accumulation of cleaved caspase-7, cleaved caspase-3, and cleaved PARP.
Accumulation of cleaved caspase-7, cleaved caspase-3, and cleaved PARP was particularly evident after 24 h of exposure.
化学情報
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分子量 298.68
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分子式 C14H7ClN4O2
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SMILES
OC1=C(C(C(C=N2)=CN3C2=C(C#N)C=N3)=O)C=C(Cl)C=C1
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Please store the product under the recommended conditions in the Certificate of Analysis.
純度とドキュメンテーション
参考文献
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
HBP-10 関連分類
HY-189745 関連分類
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