ML 2-14
Based on 1 publication(s) in Google Scholar
ML 2-14 is a PROTAC targeting BRD4 with a C4 alkyl linker. ML 2-14 consists of the E3 ligase ligand EN219 (HY-115715) (bule part), the target protein ligand JQ-1 (HY-13030) (red part), and the PROTAC linker (balck part). ML 2-14 can effectively degrade BRD4 in 231MFP breast cancer cells, and this effect can be reversed by the proteasome inhibitor Bortezomib (HY-10227) and the E1 activase inhibitor TAK-243 (HY-100487). ML 2-14 can be used for breast cancer-related research.
(Pink: BRD4(BD1BD2) ligand (HY-13030); Blue: E1/E2/E3 Enzyme ligand (HY-W002041); Black: linker (HY-W002041)).
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 99.43%
- 分子式: C40H38BrCl2N7O3S
- 分子量:847.65
-
保管条件:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
生物活性
-
化学情報
-
溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
-
Help & FAQs
MedChemExpress（MCE）の使用を引用している文献 ML 2-14
MorePROTACs アイソフォーム固有の製品をすべて表示
More
生物活性
|
BRD4 |
ML 2-14 (1 nM-1 μM; 8 h) significantly degraded BRD4 in 231MFP breast cancer cells, with DC50 values of 36 and 14 nMfor the long and short isoforms of BRD4, respectively[1].
The degradation of BRD4 by ML 2-14 (100 nM; 8 h) in 231MFP cells could be blocked by Bortezomib (4 μM; 30 min prior) and the E1 activating enzyme inhibitor TAK-243 (10 μM; 8 h prior)[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
-
性状 Solid
-
分子量 847.65
-
分子式 C40H38BrCl2N7O3S
-
Color White to off-white
-
SMILES
ClC1=CC=C(C=C1)C2=N[C@@H](CC(NCCCCOC3=CC=C(C4CC(C5=CC=C(Br)C=C5)=NN4C(CCl)=O)C=C3)=O)C6=NN=C(C)N6C7=C2C(C)=C(C)S7
-
輸送条件
Room temperature in continental US; may vary elsewhere.
-
保管条件
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (1)
-
Journal Impact Factor
-
Most Recent
-
Anal Chem
Exposome-Scale Investigation of Cl-/Br-Containing Chemicals Using High-Resolution Mass Spectrometry, Multistage Machine Learning, and Cloud Computing. [Abstract]2025 Jun 3;97(21):11099-11109. PMID: 40401576
溶剤 & 溶解度
DMSO : 100 mg/mL (117.97 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (2.95 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション
-
データシート (272 KB)
-
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
-
取扱説明書 (2659 KB)
参考文献
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.1797 mL | 5.8987 mL | 11.7973 mL | 29.4933 mL |
| 5 mM | 0.2359 mL | 1.1797 mL | 2.3595 mL | 5.8987 mL | |
| 10 mM | 0.1180 mL | 0.5899 mL | 1.1797 mL | 2.9493 mL | |
| 15 mM | 0.0786 mL | 0.3932 mL | 0.7865 mL | 1.9662 mL | |
| 20 mM | 0.0590 mL | 0.2949 mL | 0.5899 mL | 1.4747 mL | |
| 25 mM | 0.0472 mL | 0.2359 mL | 0.4719 mL | 1.1797 mL | |
| 30 mM | 0.0393 mL | 0.1966 mL | 0.3932 mL | 0.9831 mL | |
| 40 mM | 0.0295 mL | 0.1475 mL | 0.2949 mL | 0.7373 mL | |
| 50 mM | 0.0236 mL | 0.1180 mL | 0.2359 mL | 0.5899 mL | |
| 60 mM | 0.0197 mL | 0.0983 mL | 0.1966 mL | 0.4916 mL | |
| 80 mM | 0.0147 mL | 0.0737 mL | 0.1475 mL | 0.3687 mL | |
| 100 mM | 0.0118 mL | 0.0590 mL | 0.1180 mL | 0.2949 mL |
ML 2-14 関連分類
HY-132991 関連分類
Powered by Bioz