Kanamycins sulfate

Name: Kanamycins sulfate
Brand: MedChemExpress
SKU: HY-W040128
Rating: 5.0 (1 reviews)

Cat. No.: HY-W040128 Purity: 99.22%: Data Sheet
COA

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Handling Instructions
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Kanamycins sulfate is a blood-brain barrier-permeable JNK1 and Bcl-2 modulator as well as an antibiotic, with broad-spectrum antibacterial, and biofilm-inhibiting activities, and it induces autophagy. Kanamycins sulfate promotes Bcl-2 phosphorylation to upregulate autophagy levels, triggering changes such as mitochondrial swelling and endoplasmic reticulum expansion. Consequently, it causes reversible neuronal damage in the dorsal cochlear nucleus without inducing significant neuronal apoptosis. In the presence of exogenous alanine or glucose, Kanamycins sulfate effectively kills drug-resistant bacteria, restores drug sensitivity of multidrug-resistant bacteria, and alleviates urinary tract and kidney infections in mice. Kanamycins sulfate can be applied to scientific research related to Mycobacterium tuberculosis, salmonellosis, brucellosis, shigellosis, urinary tract infections, and reversible neurotoxicity.

For research use only. We do not sell to patients.

Kanamycins sulfate Chemical Structure

CAS No. : 70560-51-9

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in Water	In-stock	Estimated Time of Arrival: December 31
Solid
1 g	In-stock	Estimated Time of Arrival: December 31
5 g	In-stock	Estimated Time of Arrival: December 31
10 g	Get quote
50 g	Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Kanamycins sulfate:

Kanamycin In-stock
Kanamycin sulfate In-stock

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

JNK1

In Vitro

Kanamycins sulfate (40 μg/mL for EIB202, 30 μg/mL for ATCC15947) synergizes with exogenous alanine plus glucose to reduce survival of Edwardsiella tarda EIB202 and Edwardsiella tarda ATCC15947 in vitro biofilms by 372-fold and 162-fold, respectively^[3].
Kanamycins sulfate (0-64000 μg/mL; 6 h) synergizes with exogenous alanine plus glucose to reduce survival of multidrug-resistant Vibrio parahaemolyticus, Klebsiella pneumoniae, Pseudomonas aeruginosa, and methicillin-resistant Staphylococcus aureus (MRSA-5) cells^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Kanamycin sulfate (500 mg/kg/day; s.c.; daily; 10 days) induces reversible auditory dysfunction, transient renal impairment, and reversible DCN neuron ultrastructural damage in male Sprague-Dawley rats, with upregulated autophagy mediated via the JNK1-p-Bcl-2 pathway, and no significant induction of neuron apoptosis^[1].
Kanamycins sulfate (50 μM; 3 days) exhibits low in vivo toxicity with >90% Caenorhabditis elegans viability at 50 μM after 3 days, with no reported standalone activity against S. enteritidis in this model^[2].
Kanamycins sulfate (3000 mg/kg; i.p.; twice daily; 3 days) combined with alanine and glucose strongly suppresses urinary tract infection by multidrug-resistant Edwardsiella tarda biofilms in mice, reducing bacterial survival in catheters and kidney tissue load significantly^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	BALB/c mice (female, 4 weeks old, 20-25 g, urinary tract infection model via surgical implantation of biofilm-coated PE50 catheters)^[3]
Dosage:	3000 mg/kg
Administration:	i.p.; twice daily; 3 days
Result:	Reduced bacterial survival in catheter biofilms by >90% for both multidrug-resistant Edwardsiella tarda EIB202 and ATCC15947. Reduced kidney bacterial load by >50% for both Edwardsiella tarda strains (p < 0.01 and p < 0.05).

Molecular Weight

582.58

Formula

C₁₈H₃₈N₄O₁₅S

CAS No.

70560-51-9

Appearance

Solid

Color

White to off-white

SMILES

[Kanamycins (sulfate)]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : 62.5 mg/mL (107.28 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.7165 mL	8.5825 mL	17.1650 mL
5 mM	0.3433 mL	1.7165 mL	3.4330 mL
10 mM	0.1717 mL	0.8583 mL	1.7165 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo Dissolution Calculator

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

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Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.22%

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Data Sheet (273 KB) SDS (418 KB)

COA (232 KB) RP-HPLC (238 KB)

Handling Instructions (2659 KB)

References

[1]. Fan GR, et al. Reversible neurotoxicity of kanamycin on dorsal cochlear nucleus. Brain Res. 2013;1502:30-46.

[2]. Brezden A, et al. Dual Targeting of Intracellular Pathogenic Bacteria with a Cleavable Conjugate of Kanamycin and an Antibacterial Cell-Penetrating Peptide. J Am Chem Soc. 2016;138(34):10945-10949.

[3]. Peng B, et al. Exogenous alanine and/or glucose plus kanamycin kills antibiotic-resistant bacteria. Cell Metab. 2015;21(2):249-262.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	1.7165 mL	8.5825 mL	17.1650 mL	42.9126 mL
	5 mM	0.3433 mL	1.7165 mL	3.4330 mL	8.5825 mL
	10 mM	0.1717 mL	0.8583 mL	1.7165 mL	4.2913 mL
	15 mM	0.1144 mL	0.5722 mL	1.1443 mL	2.8608 mL
	20 mM	0.0858 mL	0.4291 mL	0.8583 mL	2.1456 mL
	25 mM	0.0687 mL	0.3433 mL	0.6866 mL	1.7165 mL
	30 mM	0.0572 mL	0.2861 mL	0.5722 mL	1.4304 mL
	40 mM	0.0429 mL	0.2146 mL	0.4291 mL	1.0728 mL
	50 mM	0.0343 mL	0.1717 mL	0.3433 mL	0.8583 mL
	60 mM	0.0286 mL	0.1430 mL	0.2861 mL	0.7152 mL
	80 mM	0.0215 mL	0.1073 mL	0.2146 mL	0.5364 mL
	100 mM	0.0172 mL	0.0858 mL	0.1717 mL	0.4291 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.