iMPZ-8
Based on 1 Customer Validation
iMPZ-8 is a β-tubulin polymerization inhibitor that inhibits β-tubulin protein expression, disrupts microtubule structure, impairs microtubule organization. iMPZ-8 inhibits proliferation and reduces cellular migration and colonization in cancer cells. iMPZ-8 induces G2/M phase arrestand induces apoptosis via the BAX-Caspase-3 intrinsic apoptotic signaling pathway. iMPZ-8 can be used for the research of cancer, suah as breast cancer, neuroblastoma and colon cancer.
연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.
- 화학식: C23H18N4O2
- 분자량:382.41
-
보관:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
용액&용해도
- Protocol
- 순도&문서
- References
-
Help & FAQs
All Caspase Isoforms
More
Biological Activity
제품 설명
IC50 & Target
[1]|
Bax |
Bcl-2 |
Caspase 3 |
In Vitro
iMPZ-8 (1-80 μM; 72 h) potently inhibits proliferation in MDA-MB-231, SH-SY5Y, and DLD-1 cancer cells with IC50 values of 7.5 μM, 9.2 μM, and 19.7 μM, respectively[1].
iMPZ-8 (3 μM; 48 h) significantly inhibits β-tubulin expression and disrupts microtubule structure in MDA-MB-231 cells, with efficacy similar to nocodazole[1].
iMPZ-8 (3 μM; 48 h) inhibits migration in MDA-MB-231 cells by approximately 50%[1].
iMPZ-8 (3 μM; 7 to 10 days) reduces colony formation in MDA-MB-231 cells by approximately 30%[1].
iMPZ-8 (3 μM; 48 h) induces G2/M phase cell cycle arrest in MDA-MB-231 cells[1].
iMPZ-8 (3 μM; 48 h) induces intrinsic apoptosis via the BAX-Caspase-3 pathway in MDA-MB-231 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:MDA-MB-231, SH-SY5Y, DLD-1
-
Concentration:1-80 μM
-
Incubation Time:72 h
-
Result:Exhibited antiproliferative activity with IC50 values of 7.5 μM in MDA-MB-231 cells, 9.2 μM in SH-SY5Y cells, and 19.7 μM in DLD-1 cells.
-
Cell Line:MDA-MB-231
-
Concentration:3 μM
-
Incubation Time:48 h
-
Result:Markedly inhibited β-tubulin protein expression, with effects comparable to Nocodazole (HY-13520) (a known β-tubulin antagonist).
-
Cell Line:MDA-MB-231
-
Concentration:3 μM
-
Incubation Time:48 h
-
Result:Induced accumulation of cells in the G2/M phase of the cell cycle, similar to Nocodazole.
-
Cell Line:MDA-MB-231
-
Concentration:3 μM
-
Incubation Time:48 h
-
Result:Induced accumulation in early and late apoptosis.
Increased BAX and Caspase 3 levels and reduced Bcl-2 levels.
- RNA extraction experimental
- Western Blot
- Detection of Apoptosis
- TUNEL staining for apoptotic DNA fragmentation
- Flow cytometric DNA-content cell-cycle staining
- Annexin V plus membrane-impermeant dye apoptosis staining
- Cell migration
- Apoptosis Solutions
- Protocol for Cell Cycle
- Breast Cancer Modeling
- Protocol For Protein Expression And Purification
- Cell Viability Determination by MTT Colorimetric Assay
Chemical Information
-
Appearance Powder
-
분자량 382.41
-
화학식 C23H18N4O2
-
SMILES
CC1=CC=C(C2=NN=C(C3=C(C4=CC=C(OC)C=C4)N=C5C=CC=CN53)O2)C=C1
-
선적
Room temperature in continental US; may vary elsewhere.
-
보관
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
용액&용해도
In Vitro:
DMSO : 3.33 mg/mL (8.71 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
순도&문서
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.6150 mL | 13.0750 mL | 26.1499 mL | 65.3749 mL |
| 5 mM | 0.5230 mL | 2.6150 mL | 5.2300 mL | 13.0750 mL |
iMPZ-8 Related Classifications
HY-181019 Related Classifications
Powered by Bioz