Cat. No.: HY-107245

Segetalin B

2 Cited Publications

고객리뷰

Based on 2 publication(s) in Google Scholar

Segetalin B, an orally active cyclopentapeptide found in Vaccaria segetalis, possesses estrogen-like activity. Segetalin B promotes mineralization of ovariectomized rat-derived bone marrow mesenchymal stem cells (BMSCs) in vitro and increases the level of osteocalcin, BMP-2, ALP, and SIRT1 activity. Segetalin B is promising for research of post-menopausal osteoporosis (PMOP).

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Purity: 99.56%
CAS No.: 164991-89-3
화학식: C₂₄H₃₂N₆O₅
분자량:484.55

보관:

Sealed storage, away from moisture and light.

Powder -80°C, 2 years , -20°C, 1 year

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Segetalin B Chemical Structure

CAS No.: 164991-89-3

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- 제품 설명
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (2)
용액&용해도
- In Vitro
- In Vivo
순도&문서
References
Help & FAQs
Bioz

Biological Activity

제품 설명

IC₅₀ & Target

SIRT1

RUNX2

All Estrogen Receptor/ERR Isoforms :

All Sirtuin Isoforms :

All RUNX Isoforms :

RUNX1
RUNX2

In Vitro

Segetalin B (0.1-100 μM, 24 h) shows significant cytotoxicity at 100 μM^[4].
Segetalin B (0.1-10 μM, 15 days) enhances the mineralization of ovariectomized rat-derived BMSCs and increases ALP activity and levels of Osteocalcin and BMP-2^[4].
Segetalin B (10 μM, 15 days) upregulates the protein expressions of Runx2 and osterix in BMSCs^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[4]

Cell Line:

Ovariectomized rat‐derived BMSCs
Concentration:

10 μM
Incubation Time:

15 days
Result:

Significantly upregulated the expression levels of Runx2 and Osterix within cells.

In Vivo

Segetalin B (2.5 mg/kg, s.c., daily for two weeks) increases the uterine weight in ovariectomized rats, showing the estrogen-like effects^[1].
Segetalin B (10-160 mg/kg, p.o., daily for 4 weeeks) inhibits bone loss in ovariectomized rats and upregulates expressions of Runx2, osterix, and SIRT1, and downregulates expressions of NICD and Hes1 in bone tissue of ovariectomized rats^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Ovariectomized rats^[1]
Dosage:

2.5 mg/kg
Administration:

s.c., daily for two weeks
Result:

Increased the uterine weight to 75.6±8.87 mg in ovariectomized rats.

Animal Model:

Ovariectomized rats^[4]
Dosage:

10-160 mg/kg
Administration:

p.o., daily for 4 weeeks
Result:

Significantly increased the calcium and phosphorus content in the urine of ovariectomized rats and upregulated the protein expressions of Runx2, Osterix, and SIRT1 in the corresponding region.

Chemical Information

CAS No. 164991-89-3
Appearance Solid
분자량 484.55
화학식 C₂₄H₃₂N₆O₅
Color White to off-white
SMILES

O=C(N[C@H](C(NCC(N[C@H](C(N[C@H](C(N1)=O)C)=O)C(C)C)=O)=O)C)[C@@H]1CC2=CNC3=CC=CC=C23
Sequence

Cyclo(Ala-Gly-Val-Ala-Trp)
Sequence Shortening

Cyclo(AGVAW)
Structure Classification
- Cyclopeptides
Initial Source
선적

Room temperature in continental US; may vary elsewhere.

보관

Sealed storage, away from moisture and light

Powder	-80°C	2 years
	-20°C	1 year

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Publications (2)

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Journal Impact Factor

Drug Dev Res

SIRT1/Notch1 signal axis involves in the promoting effect of Segetalin B on bone formation. [Abstract]

2022 Dec;83(8):1845-1857. PMID: 36207817
J Steroid Biochem Mol Biol

Segetalin B promotes bone formation in ovariectomized mice by activating PLD1/SIRT1 signaling to inhibit γ-secretase-mediated Notch1 overactivation. [Abstract]

2025 Mar:247:106669. PMID: 39736459

용액&용해도

In Vitro:

DMSO : 250 mg/mL (515.94 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.0638 mL

10.3189 mL

20.6377 mL

5 mM

0.4128 mL

2.0638 mL

4.1275 mL

10 mM

0.2064 mL

1.0319 mL

2.0638 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

몰농도 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

농도 희석 계산기

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (4.29 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (4.29 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

순도&문서

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (285 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. HiroshiMorita, et al. Segetalins B, C and D, three new cyclic peptides from Vaccaria segetalis. Tetrahedron Volume 51, Issue 21, 22 May 1995, Pages 6003-6014.

[2]. PascalSonnet, et al. First synthesis of segetalins B and G: two cyclopentapeptides with estrogen-like activity. Tetrahedron Letters. Volume 44, Issue 16, 14 April 2003, Pages 3293-3296

[3]. DONG H, et al. HPLC simultaneous determination of segetalin A and segetalin B in Vaccariae Semen[J]. Chinese Journal of Pharmaceutical Analysis, 2012, 32(5): 793-796.

[4]. Lan X, et al. SIRT1/Notch1 signal axis involves in the promoting effect of Segetalin B on bone formation. Drug Dev Res. 2022 Dec;83(8):1845-1857. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.0638 mL	10.3189 mL	20.6377 mL	51.5943 mL
	5 mM	0.4128 mL	2.0638 mL	4.1275 mL	10.3189 mL
	10 mM	0.2064 mL	1.0319 mL	2.0638 mL	5.1594 mL
	15 mM	0.1376 mL	0.6879 mL	1.3758 mL	3.4396 mL
	20 mM	0.1032 mL	0.5159 mL	1.0319 mL	2.5797 mL
	25 mM	0.0826 mL	0.4128 mL	0.8255 mL	2.0638 mL
	30 mM	0.0688 mL	0.3440 mL	0.6879 mL	1.7198 mL
	40 mM	0.0516 mL	0.2580 mL	0.5159 mL	1.2899 mL
	50 mM	0.0413 mL	0.2064 mL	0.4128 mL	1.0319 mL
	60 mM	0.0344 mL	0.1720 mL	0.3440 mL	0.8599 mL
	80 mM	0.0258 mL	0.1290 mL	0.2580 mL	0.6449 mL
	100 mM	0.0206 mL	0.1032 mL	0.2064 mL	0.5159 mL

Keywords

Segetalin B

2 Cited Publications

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Segetalin B

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Publications Citing Use of MedChemExpress (MCE) Segetalin B

All Estrogen Receptor/ERR Isoforms

All Sirtuin Isoforms

All RUNX Isoforms

Biological Activity

Chemical Information

Publications (2)

용액&용해도

순도&문서

References

Help & FAQs

Complete Stock Solution Preparation Table

Segetalin B Related Classifications

Cat. No.: HY-107245