Cat. No.: HY-N2232

N-Feruloyloctopamine

(Synonyms: N-trans-Feruloyloctopamine)

Customer Review

Based on 1 Customer Validation

N-Feruloyloctopamine (N-trans-Feruloyloctopamine) is an antioxidant component that can be isolated from garlic skin. N-Feruloyloctopamine can inhibit tumor cell proliferation and invasion, and induce apoptosis. N-Feruloyloctopamine has antitumor activity and can be used in the research of tumors such as hepatocellular carcinoma.

For research use only. We do not sell to patients.

Purity: 99.91%
CAS No.: 66648-44-0
Formula: C₁₈H₁₉NO₅
Molecular Weight:329.35

Storage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

N-Feruloyloctopamine Chemical Structure

CAS No.: 66648-44-0

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
Chemical Information
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

All Akt Isoforms :

All p38 MAPK Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
HCCLM3	IC₅₀	2.27 mM Compound: FO	Antiproliferative activity against human HCCLM3 cells assessed as reduction in cell viability after 48 hrs by CCK8 assay Antiproliferative activity against human HCCLM3 cells assessed as reduction in cell viability after 48 hrs by CCK8 assay	[PMID: 28073674]
Huh-7	IC₅₀	1.99 mM Compound: FO	Antiproliferative activity against human HuH7 cells assessed as reduction in cell viability after 48 hrs by CCK8 assay Antiproliferative activity against human HuH7 cells assessed as reduction in cell viability after 48 hrs by CCK8 assay	[PMID: 28073674]

In Vitro

N-Feruloyloctopamine (0.1-50 mM; 0-72 h) can inhibit the proliferation and invasion of Huh7 (48 h IC₅₀ of 1.99 mM) and HCCLM3 (48 h IC₅₀ of 2.27 mM) cells. The mechanism involves EMT, PI3K/Akt and p38 MAPK signaling pathways^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

Huh7 and HCCLM3 cells
Concentration:

2 mM
Incubation Time:

48 h
Result:

Did not affect the phosphorylation of p65 and ERK1/2.
Significantly decreased the phosphorylation levels of Akt and p38 MAPK.
Inhibited the level of Slug and increased the level of E-cadherin.

Chemical Information

CAS No. 66648-44-0
Appearance Solid
Molecular Weight 329.35
Formula C₁₈H₁₉NO₅
Color White to light yellow
SMILES

O=C(NCC(O)C1=CC=C(O)C=C1)/C=C/C2=CC=C(O)C(OC)=C2
Synonyms

N-trans-Feruloyloctopamine
Structure Classification
- Alkaloids
- Other Alkaloids
- Phenols
- Polyphenols
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (303.63 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.0363 mL

15.1814 mL

30.3628 mL

5 mM

0.6073 mL

3.0363 mL

6.0726 mL

10 mM

0.3036 mL

1.5181 mL

3.0363 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (7.59 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (7.59 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (7.59 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (271 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Bai ZT, et al. Inhibition of invasion by N-trans-feruloyloctopamine via AKT, p38MAPK and EMT related signals in hepatocellular carcinoma cells. Bioorg Med Chem Lett. 2017 Feb 15;27(4):989-993. [Content Brief]

[2]. Ma B, et al. N-trans-Feruloyloctopamine Wakes Up BBC3, DDIT3, CDKN1A, and NOXA Signals to Accelerate HCC Cell Apoptosis. Anal Cell Pathol (Amst). 2021 May 22;2021:1560307. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.0363 mL	15.1814 mL	30.3628 mL	75.9071 mL
	5 mM	0.6073 mL	3.0363 mL	6.0726 mL	15.1814 mL
	10 mM	0.3036 mL	1.5181 mL	3.0363 mL	7.5907 mL
	15 mM	0.2024 mL	1.0121 mL	2.0242 mL	5.0605 mL
	20 mM	0.1518 mL	0.7591 mL	1.5181 mL	3.7954 mL
	25 mM	0.1215 mL	0.6073 mL	1.2145 mL	3.0363 mL
	30 mM	0.1012 mL	0.5060 mL	1.0121 mL	2.5302 mL
	40 mM	0.0759 mL	0.3795 mL	0.7591 mL	1.8977 mL
	50 mM	0.0607 mL	0.3036 mL	0.6073 mL	1.5181 mL
	60 mM	0.0506 mL	0.2530 mL	0.5060 mL	1.2651 mL
	80 mM	0.0380 mL	0.1898 mL	0.3795 mL	0.9488 mL
	100 mM	0.0304 mL	0.1518 mL	0.3036 mL	0.7591 mL

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N-​Feruloyloctopamine

Apoptosis Compound Library

Immunology/Inflammation Compound Library

Kinase Inhibitor Library

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Mass Spectrometry Human Metabolite Library

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All Akt Isoforms

All p38 MAPK Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

N-​Feruloyloctopamine Related Classifications

N-Feruloyloctopamine

N-Feruloyloctopamine Related Classifications