Cat. No.: HY-N7264

7α-Hydroxycholesterol

3 Cited Publications

Customer Review

Based on 3 publication(s) in Google Scholar

7α-Hydroxycholesterol is a cholesterol oxide and can serve as a biomarker for oxidative stress and lipid peroxidation. 7α-Hydroxycholesterol has cytotoxic and pro-inflammatory activities. 7α-Hydroxycholesterol can also inhibit sterol synthesis and reduce the activity of HMG-CoA reductase. 7α-Hydroxycholesterol can be used in the research of diseases such as diabetes and atherosclerosis.

For research use only. We do not sell to patients.

Purity: 99.99%
CAS No.: 566-26-7
Formula: C₂₇H₄₆O₂
Molecular Weight:402.65

Storage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

7α-Hydroxycholesterol Chemical Structure

CAS No.: 566-26-7

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
Chemical Information
Publications (3)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

All Toll-like Receptor (TLR) Isoforms :

All Akt Isoforms :

All Src Isoforms :

All ERK Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
C6	IC₅₀	40 μM Compound: 4, 7alpha-OHC	Growth inhibition of rat C6 cells after 24 hrs by trypan blue-based cell counting method Growth inhibition of rat C6 cells after 24 hrs by trypan blue-based cell counting method	[PMID: 24211631]
HT-29	IC₅₀	15.5 μM Compound: 5	Cytotoxicity against human HT-29 cells after 48 hrs by Alamar Blue assay Cytotoxicity against human HT-29 cells after 48 hrs by Alamar Blue assay	[PMID: 19473028]
HT-29	IC₅₀	15.5 μM Compound: 5	Cytotoxicity against human HT-29 cells assessed as cell viability after 48 hrs by alamar blue assay Cytotoxicity against human HT-29 cells assessed as cell viability after 48 hrs by alamar blue assay	[PMID: 21797280]
HT-29	IC₅₀	15.5 μM Compound: 7	Cytotoxicity against human HT-29 cells after 48 hrs by Alamar blue assay Cytotoxicity against human HT-29 cells after 48 hrs by Alamar blue assay	[PMID: 20931970]

In Vitro

7α-Hydroxycholesterol (2.5-160 μM; 24 h) has cytotoxicity against 158N and C6 cells, with IC₅₀ values of approximately 15 μM and 40 μM, respectively^[1].
7α-Hydroxycholesterol (8 μg/mL; 2-4 h) significantly reduces the sterol synthesis rate and HMG-CoA reductase activity in primary hepatocytes or L-cell cultures^[2].
7α-Hydroxycholesterol (5 µg/mL; 48 h) increases the transcriptional level of TLR6, promotes the expression of CD14, and induces monocytes to produce chemokines such as CCL2, CCL3, and CCL4 in monocytes/macrophages. The mechanism involves the phosphorylation of Akt, Src, ERK1/2, and the p65 subunit^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Real Time qPCR^[3]

Cell Line:

THP-1 cells
Concentration:

5 µg/mL
Incubation Time:

48 h
Result:

Significantly increased the mRNA levels of TLR6 and CD14.

Chemical Information

CAS No. 566-26-7
Appearance Solid
Molecular Weight 402.65
Formula C₂₇H₄₆O₂
Color White to off-white
SMILES

CC(C)CCC[C@@H](C)[C@H]1CC[C@@]2([H])[C@]3([H])[C@H](O)C=C4C[C@@H](O)CC[C@]4(C)[C@@]3([H])CC[C@]12C
Structure Classification
- Steroids
Initial Source
- Animals
fish oil, serum
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (3)

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Journal Impact Factor

ACS Nano

Immune Modulatory Oxysterols Produced from Cholesterol-Containing Lipid Nanoparticles Regulate Tumor Growth. [Abstract]

2026 Mar 13. PMID: 41825961
Cell Chem Biol

Transient hydroxycholesterol treatment restrains TCR signaling to promote long-term immunity. [Abstract]

2024 May 16;31(5):920-931.e6. PMID: 38759618
Vet Microbiol

Identification and evaluation of Nordihydroguaiaretic acid (NDGA) as an active traditional Chinese medicine compound inhibiting the 3C-like protease of feline infectious peritonitis virus. [Abstract]

2025 Sep 15:310:110730. PMID: 40976146

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (124.18 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.4835 mL

12.4177 mL

24.8355 mL

5 mM

0.4967 mL

2.4835 mL

4.9671 mL

10 mM

0.2484 mL

1.2418 mL

2.4835 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: 10 mg/mL (24.84 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 10 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (100.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: 10 mg/mL (24.84 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 10 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (100.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 5 mg/mL (12.42 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (277 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Nury T, et al. Improved synthesis and in vitro evaluation of the cytotoxic profile of oxysterols oxidized at C4 (4α- and 4β-hydroxycholesterol) and C7 (7-ketocholesterol, 7α- and 7β-hydroxycholesterol) on cells of the central nervous system. Eur J Med Chem. 2013;70:558-67. [Content Brief]

[2]. Kandutsch AA, et al. Inhibition of sterol synthesis in cultured mouse cells by 7alpha-hydroxycholesterol, 7beta-hydroxycholesterol, and 7-ketocholesterol. J Biol Chem. 1973 Dec 25;248(24):8408-17. [Content Brief]

[3]. Son Y, et al. Glucocorticoids Impair the 7α-Hydroxycholesterol-Enhanced Innate Immune Response. Immune Netw. 2023 Oct 19;23(5):e40. [Content Brief]

[4]. Ferderbar S, et al. Cholesterol oxides as biomarkers of oxidative stress in type 1 and type 2 diabetes mellitus. Diabetes Metab Res Rev. 2007 Jan;23(1):35-42. [Content Brief]

[5]. Kitano S, et al. Oxidative status of human low density lipoprotein isolated by anion-exchange high-performance liquid chromatography--assessment by total hydroxyoctadecadienoic acid, 7-hydroxycholesterol, and 8-iso-prostaglandin F(2alpha). Anal Chim Acta. 2007 Feb 28;585(1):86-93. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.4835 mL	12.4177 mL	24.8355 mL	62.0887 mL
	5 mM	0.4967 mL	2.4835 mL	4.9671 mL	12.4177 mL
	10 mM	0.2484 mL	1.2418 mL	2.4835 mL	6.2089 mL
	15 mM	0.1656 mL	0.8278 mL	1.6557 mL	4.1392 mL
	20 mM	0.1242 mL	0.6209 mL	1.2418 mL	3.1044 mL
	25 mM	0.0993 mL	0.4967 mL	0.9934 mL	2.4835 mL
	30 mM	0.0828 mL	0.4139 mL	0.8278 mL	2.0696 mL
	40 mM	0.0621 mL	0.3104 mL	0.6209 mL	1.5522 mL
	50 mM	0.0497 mL	0.2484 mL	0.4967 mL	1.2418 mL
	60 mM	0.0414 mL	0.2070 mL	0.4139 mL	1.0348 mL
	80 mM	0.0310 mL	0.1552 mL	0.3104 mL	0.7761 mL
	100 mM	0.0248 mL	0.1242 mL	0.2484 mL	0.6209 mL

Keywords

7α-Hydroxycholesterol

3 Cited Publications

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7α-Hydroxycholesterol

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Publications Citing Use of MedChemExpress (MCE) 7α-Hydroxycholesterol

All Toll-like Receptor (TLR) Isoforms

All Akt Isoforms

All Src Isoforms

All ERK Isoforms

Biological Activity

Chemical Information

Publications (3)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

7α-Hydroxycholesterol Related Classifications

HY-N7264 Related Classifications