Ro 31-8220 formic
Based on 13 publication(s) in Google Scholar
Ro 31-8220 formic is a potent PKC inhibitor, with IC50s of 5, 24, 14, 27, 24 and 23 nM for PKCα, PKCβI, PKCβII, PKCγ, PKCε and rat brain PKC, respectively. Ro 31-8220 formic also significantly inhibits MAPKAP-K1b, MSK1, S6K1 and GSK3β (IC50s, 3, 8, 15, and 38 nM, respectively). Ro 31-8220 formic can also inhibit the expression of MKP-1, induce the expression of c-Jun, and activate JNK, and these effects possess pharmacological properties independent of PKC.
For research use only. We do not sell to patients.
- Purity: 99.76%
- Formula: C26H25N5O4S
- Molecular Weight:503.57
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Storage:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) Ro 31-8220 formic
More- Nat Commun. 2018 Sep 11;9(1):3688. [Abstract]
- J Clin Invest. 2022 Feb 15;132(4):e150101. [Abstract]
- EMBO Mol Med. 2023 Jan 11;15(1):e16373. [Abstract]
- Food Res Int. 2026 Feb 11.
- Aging Cell. 2022 Mar;21(3):e13573. [Abstract]
- Aging Cell. 2020 Oct;19(10):e13217. [Abstract]
- Front Immunol. 2021 Feb 2:11:625542. [Abstract]
- Biomolecules. 2022 Mar 10;12(3):426. [Abstract]
- FASEB J. 2019 Feb;33(2):2435-2450. [Abstract]
- Mol Med Rep. 2017 Nov;16(5):5924-5930. [Abstract]
- Life Metab. 2025 Jan 29;4(2):loaf002. [Abstract]
- Neurogastroenterol Motil. 2020 Oct;32(10):1514-1528. [Abstract]
- bioRxiv. 2024 Jul 25.
Customer Validation & Images
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WB
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WB
Biological Activity
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PKC-α 5 nM (IC50) |
PKC-βII 14 nM (IC50) |
PKC-βI 24 nM (IC50) |
PKC-ε 24 nM (IC50) |
PKC-γ 27 nM (IC50) |
Rat Brain PKC 23 nM (IC50) |
Chemical Information
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Appearance Solid
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Molecular Weight 503.57
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Formula C26H25N5O4S
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Color Orange to red
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SMILES
NC(SCCCN1C=C(C2=C(C3=CN(C)C4=C3C=CC=C4)C(NC2=O)=O)C5=C1C=CC=C5)=N.OC=O
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Synonyms
Bisindolylmaleimide IX formic
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (13)
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Journal Impact Factor
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Most Recent
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Nat Commun
Exome-wide analysis identifies three low-frequency missense variants associated with pancreatic cancer risk in Chinese populations. [Abstract]2018 Sep 11;9(1):3688. PMID: 30206226
Ro 31-8220 formic purchased from MedChemExpress. Usage Cited in: Nat Commun. 2018 Sep 11;9(1):3688. [Abstract]
Levels of phosphorylated FAK and AKT are reduced by the PKN1 inhibitors. Cells are seeded in six-well plates after transfection with PKN1 inhibitors Lestaurtinib and Ro318220 or DMSO as control.
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J Clin Invest
2022 Feb 15;132(4):e150101. PMID: 34964720 -
EMBO Mol Med
PGF2α facilitates pathological retinal angiogenesis by modulating endothelial FOS-driven ELR+ CXC chemokine expression. [Abstract]2023 Jan 11;15(1):e16373. PMID: 36511116 -
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Aging Cell
Gamma frequency light flicker regulates amyloid precursor protein trafficking for reducing β-amyloid load in Alzheimer's disease model. [Abstract]2022 Mar;21(3):e13573. PMID: 35199454 -
Aging Cell
Testicular Lmcd1 regulates phagocytosis by Sertoli cells through modulation of NFAT1/Txlna signaling pathway. [Abstract]2020 Oct;19(10):e13217. PMID: 32840323 -
Front Immunol
CBP Bromodomain Inhibition Rescues Mice From Lethal Sepsis Through Blocking HMGB1-Mediated Inflammatory Responses. [Abstract]2021 Feb 2:11:625542. PMID: 33603756 -
Biomolecules
Ketamine Improves Desensitization of µ-Opioid Receptors Induced by Repeated Treatment with Fentanyl but Not with Morphine. [Abstract]2022 Mar 10;12(3):426. PMID: 35327617 -
FASEB J
Bile acids induce visceral hypersensitivity via mucosal mast cell-to-nociceptor signaling that involves the farnesoid X receptor/nerve growth factor/transient receptor potential vanilloid 1 axis. [Abstract]2019 Feb;33(2):2435-2450. PMID: 30260705 -
Mol Med Rep
2017 Nov;16(5):5924-5930. PMID: 28849166
Ro 31-8220 formic purchased from MedChemExpress. Usage Cited in: Mol Med Rep. 2017 Nov;16(5):5924-5930. [Abstract]
The PKC inhibitor Ro 31 8220 is used to investigate the implication of PKC mediated signaling pathways.
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Life Metab
Intermittent fasting inhibits platelet activation and thrombosis through the intestinal metabolite indole-3-propionate. [Abstract]2025 Jan 29;4(2):loaf002. PMID: 40078933 -
Neurogastroenterol Motil
Acute stress induces visceral hypersensitivity via glucocorticoid receptor-mediated membrane insertion of TRPM8: Involvement of a non-receptor tyrosine kinase Pyk2. [Abstract]2020 Oct;32(10):1514-1528. PMID: 32391653 -
Solvent & Solubility
DMSO : ≥ 100 mg/mL (198.58 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (278 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Wilkinson SE, et al. Isoenzyme specificity of bisindolylmaleimides, selective inhibitors of protein kinase C. Biochem J. 1993 Sep 1;294 (Pt 2):335-7. [Content Brief]
[2]. Davies SP, et al. Specificity and mechanism of action of some commonly used protein kinase inhibitors. Biochem J. 2000 Oct 1;351(Pt 1):95-105. [Content Brief]
[3]. Tian F, et al. Inhibition of protein kinase C protects against paraoxon-mediated neuronal cell death. Neurotoxicology. 2007 Jul;28(4):843-9. Epub 2007 Apr 20. [Content Brief]
[4]. Hambleton M, et al. Pharmacological- and gene therapy-based inhibition of protein kinase Calpha/beta enhances cardiac contractility and attenuates heart failure. Circulation. 2006 Aug 8;114(6):574-82. [Content Brief]
[5]. Beltman J, et al. The selective protein kinase C inhibitor, Ro-31-8220, inhibits mitogen-activated protein kinase phosphatase-1 (MKP-1) expression, induces c-Jun expression, and activates Jun N-terminal kinase. J Biol Chem. 1996 Oct 25;271(43):27018-24. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.9858 mL | 9.9291 mL | 19.8582 mL | 49.6455 mL |
| 5 mM | 0.3972 mL | 1.9858 mL | 3.9716 mL | 9.9291 mL | |
| 10 mM | 0.1986 mL | 0.9929 mL | 1.9858 mL | 4.9646 mL | |
| 15 mM | 0.1324 mL | 0.6619 mL | 1.3239 mL | 3.3097 mL | |
| 20 mM | 0.0993 mL | 0.4965 mL | 0.9929 mL | 2.4823 mL | |
| 25 mM | 0.0794 mL | 0.3972 mL | 0.7943 mL | 1.9858 mL | |
| 30 mM | 0.0662 mL | 0.3310 mL | 0.6619 mL | 1.6549 mL | |
| 40 mM | 0.0496 mL | 0.2482 mL | 0.4965 mL | 1.2411 mL | |
| 50 mM | 0.0397 mL | 0.1986 mL | 0.3972 mL | 0.9929 mL | |
| 60 mM | 0.0331 mL | 0.1655 mL | 0.3310 mL | 0.8274 mL | |
| 80 mM | 0.0248 mL | 0.1241 mL | 0.2482 mL | 0.6206 mL | |
| 100 mM | 0.0199 mL | 0.0993 mL | 0.1986 mL | 0.4965 mL |
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