Cat. No.: HY-N1419

Vaccarin

1 Cited Publications

Customer Review

Based on 1 publication(s) in Google Scholar

Vaccarin is an orally active flavonoid glycoside with multiple biological functions. Vaccarin promotes neovascularization by activating AKT and ERK. Vaccarin activates the AMPK signaling pathway to improve insulin resistance and steatosis. Vaccarin is a MAPK, NF-κB, and NFAT inhibitor, effectively blocking RANKL-induced osteoclastogenesis.

For research use only. We do not sell to patients.

Purity: 99.05%
CAS No.: 53452-16-7
Formula: C₃₂H₃₈O₁₉
Molecular Weight:726.63

Storage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Vaccarin Chemical Structure

CAS No.: 53452-16-7

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Publications Citing Use of MedChemExpress (MCE) Vaccarin

Phytomedicine. 2025 Jun:141:156697. [Abstract]

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Cell Proliferation/Viability AssayWBIFIHCRT-PCR

Cell Proliferation/Viability Assay
WB
IF
IHC
RT-PCR

Biological Activity
- Description
- In Vitro
- In Vivo
Chemical Information
Publications (1)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

All AMPK Isoforms :

All Akt Isoforms :

All ERK Isoforms :

All p38 MAPK Isoforms :

All NF-κB Isoforms :

All JNK Isoforms :

In Vitro

Vaccarin (0.54-34.4 μM, 48 h) increases the proliferation activity of human microvascular endothelial cells-1 (HMEC-1)^[1].
Vaccarin (1.08-2.15 μM, 24-48 h) promotes HMEC-1 migration and capillary lumen formation^[1].
Vaccarin (5 μM, 24 h) activites insulin resistance in HepG2 cells by inhibiting gluconeogenesis and promoting glycogen synthesis^[2].
Vaccarin (5 μM, 24 h) activates AMPK to increase insulin sensitivity and inhibit steatosis in HepG2 cells^[2].
Vaccarin (4-64 μM, 48 h) inhibits RANKL-induced osteoclastogenesis in bone marrow macrophages (BMMs)^[3].
Vaccarin (8 μM, 4 h) inhibits osteocalcin (OC) differentiation by inhibiting MAPK and NF-κB signaling pathways^[3].
Vaccarin (4-16 μM) reduces the expression of OC marker genes by inhibiting the activation of NF-κB and NFATc1^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[2]

Cell Line:

HepG2 cells
Concentration:

5 μM
Incubation Time:

24 h
Result:

Down-regulated the protein levels of PEPCK, G6Pase, p-GS, SREBP-1 and FAS.
Up-regulated the protein levels of p-FOXO1, p-GSK3β, p-AMPK and p-ACC.

Western Blot Analysis^[3]

Cell Line:

BMM
Concentration:

8 μM
Incubation Time:

4 h
Result:

Down-regulated the protein expression levels of IκB-ɑ, p-NF-κBp65, p-p38, p-JNK, p-ERK, c-fos, and NFATc1.

In Vivo

Vaccarin (5/10 mg/kg, p.o., once per day for 2 weeks) increases epidermal growth factor (EGF)-induced angiogenesis in the mouse matrigel plug model^[1].
Vaccarin (1 mg/kg, i.p., once per day for 4 weeks) inhibits insulin resistance and glucose/lipid metabolism disorders in the type 2 diabetes (T2DM) mouse model^[2].
Vaccarin (2.5/5 mg/kg, once every 2 days for 14 days) inhibits osteolytic bone loss in the Ti particle-induced calvarial osteolysis mouse model^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Mouse matrigel plug model (Female ICR mice, aged 4–5 weeks old and weighing 17-20 g; subcutaneously injected with 500 μl of Matrigel containing heparin (113 units) and mouse EGF (120 ng) into the left hypogastric region)^[1]
Dosage:

5 and 10 mg/kg
Administration:

Oral gavage (p.o.), once per day for 2 weeks
Result:

Increased the number of endothelial cells.
Up-regulated the expression of p-Erk and p-Akt in blood vessels.

Animal Model:

T2DM mouse model (Six-week-old male C57BL/6 J mice, intraperitoneal injection of STZ (HY-13753) and HFD)^[2]
Dosage:

1 mg/kg
Administration:

Intraperitoneal injection (i.p.), once per day for 4 weeks
Result:

Increased liver glycogen levels in mice.
Down-regulated the ratio of liver weight (LW) to body weight (BW), p-IRS-1 (Ser 307), TC, TG, ALT, AST, LDL-C and NEFA levels in the liver of mice.

Chemical Information

CAS No. 53452-16-7
Appearance Solid
Molecular Weight 726.63
Formula C₃₂H₃₈O₁₉
Color Light yellow to yellow
SMILES

O=C1C=C(C2=CC=C(O[C@H]3[C@@H]([C@H]([C@@H]([C@@H](CO)O3)O)O)O)C=C2)OC4=CC(O)=C([C@H]5[C@@H]([C@H]([C@@H]([C@@H](CO)O5)O)O)O[C@H]6[C@@H]([C@H]([C@H](CO6)O)O)O)C(O)=C14
Structure Classification
- Flavonoids
- Flavones
- Phenols
- Polyphenols
Initial Source
- Plants
- other families
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (1)

All Cell Proliferation/Viability AssayWBIFIHCRT-PCR

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Phytomedicine

Vaccarin ameliorates osteoarthritis by suppressing the c-Jun N-terminal kinase (JNK)-serum amyloid A2 (SAA2) pathway mediating chondrocyte senescence. [Abstract]

2025 Jun:141:156697. PMID: 40215820

Vaccarin purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Jun:141:156697. [Abstract]

CCK-8 assay showed no cytotoxicity up to 40 μM after 24 and 48 h treated with Vaccarin.

Vaccarin purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Jun:141:156697. [Abstract]

Western blot indicated Vaccarin dose-dependently restored ECM proteins including Aggrecan, Collagen II, and SOX9 suppressed by IL-1β treated with Vaccarin (5, 10, 20, 40 μM).

Vaccarin purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Jun:141:156697. [Abstract]

Immunofluorescence confirmed that Vaccarin (40 μM) restored IL-1β-reduced Collagen II and MMP13 levels.

Vaccarin purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Jun:141:156697. [Abstract]

Immunohistochemical staining Aggrecan and MMP13 treated with Vaccarin (25, 50 mg/kg, a intra-articular injection).

Vaccarin purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Jun:141:156697. [Abstract]

RT-qPCR analysis showing reduced SAA2 expression after Vaccarin (40 μM) or SP600125 treatment.

Solvent & Solubility

In Vitro:

DMSO : 125 mg/mL (172.03 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.3762 mL

6.8811 mL

13.7622 mL

5 mM

0.2752 mL

1.3762 mL

2.7524 mL

10 mM

0.1376 mL

0.6881 mL

1.3762 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (2.86 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (2.86 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (2.86 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (282 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Xie F, et al. Vaccarin promotes endothelial cell proliferation in association with neovascularization in vitro and in vivo. Mol Med Rep. 2015 Jul;12(1):1131-6. [Content Brief]

[2]. Lei Y, et al. Vaccarin ameliorates insulin resistance and steatosis by activating the AMPK signaling pathway. Eur J Pharmacol. 2019 May 15;851:13-24. [Content Brief]

[3]. Liu Y, et al. Vaccarin prevents titanium particle-induced osteolysis and inhibits RANKL-induced osteoclastogenesis by blocking NF-κB and MAPK signaling pathways. J Cell Physiol. 2019 Aug;234(8):13832-13842. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.3762 mL	6.8811 mL	13.7622 mL	34.4054 mL
	5 mM	0.2752 mL	1.3762 mL	2.7524 mL	6.8811 mL
	10 mM	0.1376 mL	0.6881 mL	1.3762 mL	3.4405 mL
	15 mM	0.0917 mL	0.4587 mL	0.9175 mL	2.2937 mL
	20 mM	0.0688 mL	0.3441 mL	0.6881 mL	1.7203 mL
	25 mM	0.0550 mL	0.2752 mL	0.5505 mL	1.3762 mL
	30 mM	0.0459 mL	0.2294 mL	0.4587 mL	1.1468 mL
	40 mM	0.0344 mL	0.1720 mL	0.3441 mL	0.8601 mL
	50 mM	0.0275 mL	0.1376 mL	0.2752 mL	0.6881 mL
	60 mM	0.0229 mL	0.1147 mL	0.2294 mL	0.5734 mL
	80 mM	0.0172 mL	0.0860 mL	0.1720 mL	0.4301 mL
	100 mM	0.0138 mL	0.0688 mL	0.1376 mL	0.3441 mL

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Vaccarin

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