Cat. No.: HY-12557

γ-Glutamylvaline

(Synonyms: γ-Glu-Val)

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γ‑Glutamylvaline (γ-Glu-Val) is a calcium‑sensing receptor (CaSR) agonist. γ‑Glutamylvaline activates CaSR and facilitates its binding to β‑arrestin 2 to modulate inflammatory and metabolic homeostasis signaling. γ‑Glutamylvaline inhibits TNF‑α‑induced IL‑6/MCP‑1 and enhances adiponectin/PPARγ in adipocytes. γ‑Glutamylvaline upregulates Wnt5a, restores β‑catenin phosphorylation, and reduces serine‑phosphorylated IRS‑1 in adipocytes. γ-Glutamylvaline can be used for the research of low-grade chronic inflammation.

For research use only. We do not sell to patients.

Purity: 99.94%
CAS No.: 2746-34-1
Formula: C₁₀H₁₈N₂O₅
Molecular Weight:246.26

Storage:

Sealed storage, away from moisture.

Powder -80°C, 2 years , -20°C, 1 year

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

γ-Glutamylvaline Chemical Structure

CAS No.: 2746-34-1

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target^[1]

PPAR-γ

IL-6

Wnt5A

All Endogenous Metabolite Isoforms :

All Wnt Isoforms :

All TNF Receptor Isoforms :

All Interleukin Related Isoforms :

All PPAR Isoforms :

In Vitro

γ-Glutamylvaline (0.01-10 μM; 6 h) activates CaSR in differentiated 3T3-L1 mouse adipocytes, as measured by increased intracellular calcium fluorescence after 6 h of incubation^[1].
γ-Glutamylvaline (1-100 μM; 24 h pretreatment, 24 h TNF-α exposure) significantly suppresses TNF-α-induced IL-6 and MCP-1 production and restores adiponectin levels in differentiated 3T3-L1 mouse adipocytes^[1].
γ-Glutamylvaline (10 μM; 2 h) promotes the binding interaction between CaSR and β-arrestin 2 in differentiated 3T3-L1 mouse adipocytes^[1].
γ-Glutamylvaline (10 μM; 2 h) upregulates Wnt5a gene expression and restores TNF-α-suppressed β-catenin phosphorylation in differentiated 3T3-L1 mouse adipocytes^[1].
γ-Glutamylvaline (10 μM; 2 h pretreatment, 30 min TNF-α stimulation) counteracts TNF-α-induced downregulation of PPARγ1+2 in differentiated 3T3-L1 mouse adipocytes^[1].
γ-Glutamylvaline (10 μM; 45 min insulin exposure, 2 h treatment, 30 min TNF-α stimulation) inhibits TNF-α-induced serine phosphorylation of IRS-1 in differentiated 3T3-L1 mouse adipocytes^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

ELISA Assay^[1]

Cell Line:

differentiated 3T3-L1 mouse adipocytes (TNF-α-induced inflammation model)
Concentration:

1, 10, 100 μM; 10 μM (IL-6 suppression); 10, 100 μM (MCP-1 suppression; adiponectin restoration)
Incubation Time:

24 h (pretreatment); 24 h (TNF-α exposure)
Result:

Suppressed TNF-α-induced IL-6 production to 23.92 ng/mL at 10 μM.
Suppressed TNF-α-induced MCP-1 production to 101.17 ng/mL and 120.36 ng/mL at 10 μM and 100 μM, respectively.
Restored adiponectin production to 7.37 ng/mL and 7.40 ng/mL at 10 μM and 100 μM, respectively.

Western Blot Analysis^[1]

Cell Line:

differentiated 3T3-L1 mouse adipocytes (TNF-α-induced model)
Concentration:

10 μM
Incubation Time:

2 h (pretreatment); 30 min (TNF-α stimulation)
Result:

Counteracted TNF-α-induced downregulation of PPARγ1/PPARγ2, increasing expression to 1.249 relative to β-actin at 10 μM.
This effect was reversed by pretreatment with the CaSR antagonist NPS-2143.

Western Blot Analysis^[1]

Cell Line:

differentiated 3T3-L1 mouse adipocytes
Concentration:

10 μM
Incubation Time:

45 min (insulin exposure); 2 h (γ-glutamylvaline treatment); 30 min (TNF-α stimulation)
Result:

Inhibited TNF-α-upregulated p-Ser-IRS1 expression to 1.89 relative to total IRS-1 at 10 μM.

Chemical Information

CAS No. 2746-34-1
Appearance Solid
Molecular Weight 246.26
Formula C₁₀H₁₈N₂O₅
Color White to off-white
Synonyms

γ-Glu-Val
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture

Powder	-80°C	2 years
	-20°C	1 year

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : ≥ 100 mg/mL (406.07 mM)

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

4.0607 mL

20.3037 mL

40.6075 mL

5 mM

0.8121 mL

4.0607 mL

8.1215 mL

10 mM

0.4061 mL

2.0304 mL

4.0607 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (280 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Xing L, et al. γ-Glutamylvaline Prevents Low-Grade Chronic Inflammation via Activation of a Calcium-Sensing Receptor Pathway in 3T3-L1Mouse Adipocytes. J Agric Food Chem. 2019 Jul 31;67(30):8361-8369 [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O	1 mM	4.0607 mL	20.3037 mL	40.6075 mL	101.5187 mL
	5 mM	0.8121 mL	4.0607 mL	8.1215 mL	20.3037 mL
	10 mM	0.4061 mL	2.0304 mL	4.0607 mL	10.1519 mL
	15 mM	0.2707 mL	1.3536 mL	2.7072 mL	6.7679 mL
	20 mM	0.2030 mL	1.0152 mL	2.0304 mL	5.0759 mL
	25 mM	0.1624 mL	0.8121 mL	1.6243 mL	4.0607 mL
	30 mM	0.1354 mL	0.6768 mL	1.3536 mL	3.3840 mL
	40 mM	0.1015 mL	0.5076 mL	1.0152 mL	2.5380 mL
	50 mM	0.0812 mL	0.4061 mL	0.8121 mL	2.0304 mL
	60 mM	0.0677 mL	0.3384 mL	0.6768 mL	1.6920 mL
	80 mM	0.0508 mL	0.2538 mL	0.5076 mL	1.2690 mL
	100 mM	0.0406 mL	0.2030 mL	0.4061 mL	1.0152 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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γ-Glutamylvaline

Apoptosis Compound Library

Cell Cycle/DNA Damage Compound Library

GPCR/G Protein Compound Library

Immunology/Inflammation Compound Library

Metabolism/Protease Compound Library

Stem Cell Signaling Compound Library

Wnt/Hedgehog/Notch Compound Library

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Mass Spectrometry Human Metabolite Library

Lactylation Compound Library

All Endogenous Metabolite Isoforms

All Wnt Isoforms

All TNF Receptor Isoforms

All Interleukin Related Isoforms

All PPAR Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

γ-Glutamylvaline Related Classifications

HY-12557 Related Classifications