7-Methylguanine
Based on 1 Customer Validation
7-Methylguanine is an orally active and competitive PARP-1 inhibitor with a Ki value of 61 μM. 7-Methylguanine is a metabolite of nucleic acids. 7-Methylguanine has anticancer activity against uterine sarcoma and colon adenocarcinoma. 7-Methylguanine is used as a probe for protein-DNA interactions.
For research use only. We do not sell to patients.
- Purity: 99.97%
- CAS No.: 578-76-7
- Formula: C6H7N5O
- Molecular Weight:165.15
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
All Endogenous Metabolite Isoforms
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Biological Activity
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Human Endogenous Metabolite |
PARP-1 61 μM (Ki) |
7-Methylguanine (0-200 μM) inhibits PARP1 enzymatic activity in a dose-dependent manner[1].
7-Methylguanine is a competitive PARP-1 inhibitor with a Ki value of 61 μM[2].
7-Methylguanine (0-450 μM) inhibits the dissociation of the PARP-1-DNA complex due to suppression of PARP-1 catalytic activity[2].
7-Methylguanine (0.02-0.2 mg/mL; 24 h) does not produce DNA damage in immortalized human kidney epithelial cells (NKE-hTERT)[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
7-Methylguanine (50 mg/kg; i.g.; 3 times per week) effectively penetrates tumor tissue and suppresses colon adenocarcinoma growth in BALB/c mice with transplanted Akatol tumor[5].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:CBA, BALB/c, C57BL/6 mice (6-week-old); toxicology study and carcinogenicity study[3]
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Dosage:50 mg/kg, 200 mg/kg, 600 mg/kg
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Administration:Intragastric administration, 3 times per week for 4 weeks (for 50 mg/kg group), 3 times per week for 1 week (for 200 mg/kg group), single dose (for 600 mg/kg group)
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Result:Exerted no adverse effects or changes in morphology (at 50 mg/kg for 3 times per week up to 4 weeks).
Caused some lymphoid infiltrates in lungs, liver and spleen, and focal lymphoid hyperplasia in intestines in CBA mice (at 600 mg/kg single dose).
Did not induce mutations or structural chromosomal abnormalities, and had no blastomogenic activity.
Significantly inhibited tumor growth in CBA mice with uterine sarcoma US-322 (at 50 mg/kg 3 times per week).
Had an antitumor effect in CBA mice with cervical squamous cell carcinoma RShM-5 (alone for 1 week).
Inhibited tumor growth in combination with Cisplatin (HY-17394) within a week after inoculation.
Chemical Information
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CAS No. 578-76-7
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Appearance Solid
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Molecular Weight 165.15
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Formula C6H7N5O
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Color White to light yellow
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SMILES
O=C1NC(N)=NC2=C1N(C)C=N2
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
1 M NaOH : ≥ 100 mg/mL (605.51 mM)
H2O : 4.72 mg/mL (28.58 mM; ultrasonic and adjust pH to 11 with 1 M NaOH)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (277 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Kurgina TA, et al. Inhibitory Effects of 7-Methylguanine and Its Metabolite 8-Hydroxy-7-Methylguanine on Human Poly(ADP-Ribose) Polymerase 1. Biochemistry (Mosc). 2022 Aug;87(8):823-831. [Content Brief]
[2]. Nilov D, et al. Molecular Mechanisms of PARP-1 Inhibitor 7-Methylguanine. Int J Mol Sci. 2020 Mar 20;21(6):2159. [Content Brief]
[3]. Kirsanov K, et al. Toxicological Properties of 7-Methylguanine, and Preliminary Data on its Anticancer Activity. Front Pharmacol. 2022 Jul 6;13:842316. [Content Brief]
[4]. Gaubatz JW, et al. Introduction, distribution, and removal of 7-methylguanine in different liver chromatin fractions of young and old mice. Mutat Res. 1997 Apr 14;375(1):25-35. [Content Brief]
[5]. Kirsanov K, et al. 7-Methylguanine Inhibits Colon Cancer Growth in Vivo. Acta Naturae. 2024 Apr-Jun;16(2):50-52. [Content Brief]
[6]. Lee S, et al. Synthesis and structure of duplex DNA containing the genotoxic nucleobase lesion N7-methylguanine. J Am Chem Soc. 2008 Sep 3;130(35):11570-1. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| H2O / 1 M NaOH | 1 mM | 6.0551 mL | 30.2755 mL | 60.5510 mL | 151.3775 mL |
| 5 mM | 1.2110 mL | 6.0551 mL | 12.1102 mL | 30.2755 mL | |
| 10 mM | 0.6055 mL | 3.0276 mL | 6.0551 mL | 15.1378 mL | |
| 15 mM | 0.4037 mL | 2.0184 mL | 4.0367 mL | 10.0918 mL | |
| 20 mM | 0.3028 mL | 1.5138 mL | 3.0276 mL | 7.5689 mL | |
| 25 mM | 0.2422 mL | 1.2110 mL | 2.4220 mL | 6.0551 mL | |
| 1 M NaOH | 30 mM | 0.2018 mL | 1.0092 mL | 2.0184 mL | 5.0459 mL |
| 40 mM | 0.1514 mL | 0.7569 mL | 1.5138 mL | 3.7844 mL | |
| 50 mM | 0.1211 mL | 0.6055 mL | 1.2110 mL | 3.0276 mL | |
| 60 mM | 0.1009 mL | 0.5046 mL | 1.0092 mL | 2.5230 mL | |
| 80 mM | 0.0757 mL | 0.3784 mL | 0.7569 mL | 1.8922 mL | |
| 100 mM | 0.0606 mL | 0.3028 mL | 0.6055 mL | 1.5138 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
7-Methylguanine Related Classifications
HY-113352 Related Classifications
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