Cat. No.: HY-N0167

Gynostemma Extract

(Synonyms: Ginsenoside C-Mx1; Gypenoside IX)

1 Cited Publications

Customer Review

Based on 1 publication(s) in Google Scholar

Gynostemma Extract (Gypenoside IX) is a triterpenoid saponin. Gynostemma Extract can be isolated from Panax notoginseng. Gynostemma Extract reduces NFκB nuclear translocation and transcriptional activity by inhibiting the p38 MAPK/Akt/NFκB signaling pathway. Gynostemma Extract reduces the expression of cytokines (COX-2, IL-6) and the production of NO. Gynostemma Extract has anti-inflammatory activity. Gynostemma Extract can be used in the study of liver injury and neuroinflammation.

For research use only. We do not sell to patients.

Purity: 99.92%
CAS No.: 80321-63-7
Formula: C₄₇H₈₀O₁₇
Molecular Weight:917.13

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Gynostemma Extract Chemical Structure

CAS No.: 80321-63-7

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Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
- Clinical Trial
Chemical Information
Publications (1)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target^[1]^[2]

IL-6

COX-2

All p38 MAPK Isoforms :

All Akt Isoforms :

All NF-κB Isoforms :

All COX Isoforms :

All Interleukin Related Isoforms :

All NO Synthase Isoforms :

In Vitro

Gynostemma Extract (3.125-50 μM; 24 h) shows no cytotoxicity on C6 cells^[2].
Gynostemma Extract (3.125-50 μM; 2 h) dose-dependently reduces NO production in C6 cells stimulated by LPS and TNF-α, with an IC₅₀ of around 25 μM ^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Gynostemma Extract (30 mg/kg; i.p.) reduces the production of TNF-α, IL-6, and iNOS at protein levels, alleviates astrogliosis, and impedes the elevation of GFAP in brain cortex of LPS-induced C57BL/6 mice^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Five-week-old male C57BL/6 mice with LPS-induced neuroinflammation ^[2]
Dosage:

3 mg/kg, 10 mg/kg, 30 mg/kg
Administration:

Intraperitoneal injection
Result:

Prevented the increase of mRNA expression of inflammatory genes such as iNOS, TNF-α, IL-6, and COX-2 in brain cortex.
Reduced the production of TNF-α, IL-6, and iNOS at protein levels, alleviated astrogliosis, and impeded the elevation of GFAP.
Attenuated the phosphorylation of NFκB, IκB, p38 MAPK, and Akt in brain cortex.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS No. 80321-63-7
Appearance Solid
Molecular Weight 917.13
Formula C₄₇H₈₀O₁₇
Color White to off-white
SMILES

O[C@H]1[C@H](O)[C@@H](CO)O[C@@H](O[C@@H]2C(C)(C)[C@@](CC[C@]3(C)[C@]4([H])C[C@@H](O)[C@@]5([H])[C@@]3(C)CC[C@@H]5[C@](O[C@@H]6O[C@H](CO[C@@H]7OC[C@@H](O)[C@H](O)[C@H]7O)[C@@H](O)[C@H](O)[C@H]6O)(C)CC/C=C(C)\C)([H])[C@]4(C)CC2)[C@@H]1O
Synonyms

Ginsenoside C-Mx1; Gypenoside IX
Structure Classification
- Terpenoids
- Triterpenes
Initial Source
- Endogenous metabolite
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (1)

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Toxicon

The impact of abamectin treatment on oxidative stress and the caspase-3/caspase-8/caspase-9/Bcl-2/Bax/Fas/p53 mediated apoptotic pathway: Role of baicalin and vitamin E. [Abstract]

2026 Jan:269:108664. PMID: 41260455

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (109.04 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.0904 mL

5.4518 mL

10.9036 mL

5 mM

0.2181 mL

1.0904 mL

2.1807 mL

10 mM

0.1090 mL

0.5452 mL

1.0904 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: 2.5 mg/mL (2.73 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (2.73 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (2.73 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (280 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Yoshikawa M, et al. Structures of new dammarane-type Triterpene Saponins from the flower buds of Panax notoginseng and hepatoprotective effects of principal Ginseng Saponins. J Nat Prod. 2003 Jul;66(7):922-7. [Content Brief]

[2]. Wang X, et al. Gypenoside IX Suppresses p38 MAPK/Akt/NFκB Signaling Pathway Activation and Inflammatory Responses in Astrocytes Stimulated by Proinflammatory Mediators. Inflammation. 2017 Dec;40(6):2137-2150. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.0904 mL	5.4518 mL	10.9036 mL	27.2589 mL
	5 mM	0.2181 mL	1.0904 mL	2.1807 mL	5.4518 mL
	10 mM	0.1090 mL	0.5452 mL	1.0904 mL	2.7259 mL
	15 mM	0.0727 mL	0.3635 mL	0.7269 mL	1.8173 mL
	20 mM	0.0545 mL	0.2726 mL	0.5452 mL	1.3629 mL
	25 mM	0.0436 mL	0.2181 mL	0.4361 mL	1.0904 mL
	30 mM	0.0363 mL	0.1817 mL	0.3635 mL	0.9086 mL
	40 mM	0.0273 mL	0.1363 mL	0.2726 mL	0.6815 mL
	50 mM	0.0218 mL	0.1090 mL	0.2181 mL	0.5452 mL
	60 mM	0.0182 mL	0.0909 mL	0.1817 mL	0.4543 mL
	80 mM	0.0136 mL	0.0681 mL	0.1363 mL	0.3407 mL
	100 mM	0.0109 mL	0.0545 mL	0.1090 mL	0.2726 mL

Keywords

Gynostemma Extract

1 Cited Publications

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Gynostemma Extract

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Publications Citing Use of MedChemExpress (MCE) Gynostemma Extract

All p38 MAPK Isoforms

All Akt Isoforms

All NF-κB Isoforms

All COX Isoforms